BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0571.0017.p (446 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K655.1 B3K655_9BACI Glycosyl hydrolase family 32 domain protein 288 6e-76 C5EMA4.1 C5EMA4_9FIRM Glycoside hydrolase 249 4e-64 A1R3C5.1 A1R3C5_ARTAT Putative glycosyl hydrolases family 32 pro... 248 1e-63 C4GDD6.1 C4GDD6_9FIRM Putative uncharacterized protein 239 5e-61 C2KX06.1 C2KX06_9FIRM Possible beta-fructofuranosidase 224 1e-56 >B3K655.1 B3K655_9BACI Glycosyl hydrolase family 32 domain protein Length = 474 Score = 288 bits (738), Expect = 6e-76, Method: Compositional matrix adjust. Identities = 177/474 (37%), Positives = 253/474 (53%), Gaps = 50/474 (10%) Query: 1 MPFYHEGVYHLYYLH----------KYCIYEVATKDFVSYGEPRLAVVCGSPEEQDWHIG 50 +P+Y +G + L+YLH + Y V TKDFV+Y E + G G Sbjct: 17 IPYYEDGEFKLFYLHGWRDNYREGLDHGWYLVGTKDFVNYREEGPCKIEG---------G 67 Query: 51 TGSVVERDGVFHFYYTGFNENNRDTEGKHEQVLMRAISDDLIQWRKDPAFFVPPYTERYG 110 TG +++ DG++H +Y F +Q++ AIS DL W P Y Sbjct: 68 TGHILKVDGIYHMFYCIFPPG--------KQLVCHAISRDLKTWEPIPEDTFAADDRIYE 119 Query: 111 GLHWRDPQVFWNDQAGEYWMAVTTTEKDGPMNRSGCTHIMRSADLEQWKHGEMIYAPRSY 170 WRDP VFWN++ G+YWM ++ K GP NR GCT ++ S DL+ W++ E +YAP + Sbjct: 120 LSDWRDPFVFWNEEEGQYWMLLSAMAK-GPTNRKGCTALLSSKDLKAWEYREPLYAPNLH 178 Query: 171 A-THECHDIFQMGGWWYMVFSNYTRWWETRYRMAKSPEGPWFIPPYDDMFDGRGFYAAKT 229 HEC D+F++G WWY+++S+YT + T YRM++S GPW I P +D FDGR FYAAK+ Sbjct: 179 VGAHECPDLFRIGDWWYLIYSSYTGRFATFYRMSRSLNGPW-ITPKEDTFDGRAFYAAKS 237 Query: 230 VSNGTNRYLVGWQAI-----------RSNMDDMSPYQWGGSTLVHELVQHPDGTLGVQLI 278 V++G RYL GW +S D + WGG+ +VHE++Q PDGTLGV++ Sbjct: 238 VADGQKRYLFGWNPTKNDDLFGWNPPKSQGKDYDTWDWGGNLVVHEIIQRPDGTLGVKVP 297 Query: 279 PAIQDSFRQELPQAPAAHLGVWDSDTAAITGKEPYGF-GWTELGRMAGDCLIEASVQWSS 337 + +F++ +P G W +++ + PYGF G T + C I ASV +S Sbjct: 298 DTVDAAFKELMPVRFNRVEGAWLDSDCSLSCESPYGFAGCTTREDLPDQCKISASVSFSG 357 Query: 338 DTQACGIMLHTSGAGLEKWCQVRLEPQRNRLIFDR--SNKFDGHHLF---VE-ERYLPLD 391 TQ G ML T L+ + EPQRNRL F +G F VE ER + L Sbjct: 358 QTQGLGFMLRTEDT-LDAAYYITFEPQRNRLTFRGYIMQSEEGGKTFPYEVELERPIELI 416 Query: 392 GSSGADIKIVASGNIIIIYVN-NTALATRAYNYEPGSFGLFVEHGEAAFSNVKI 444 +IK+ G+I IYV+ N A++ R Y+ + G GLFV G A FS+V+I Sbjct: 417 PERPYEIKVFIDGSICEIYVDENIAMSARMYDIQHGKLGLFVSQGSAQFSDVRI 470 >C5EMA4.1 C5EMA4_9FIRM Glycoside hydrolase Length = 481 Score = 249 bits (636), Expect = 4e-64, Method: Compositional matrix adjust. Identities = 156/469 (33%), Positives = 238/469 (50%), Gaps = 30/469 (6%) Query: 1 MPFYHEGVYHLYYLHKYCI-----------YEVATKDFVSYGEPRLAVVCGSPEEQDWHI 49 +P+Y +G Y+ YYLH + + TKD + A+ G + + +I Sbjct: 17 IPYYEDGTYYAYYLHDPRVNPGEFAEETTWHLATTKDCRKFQYQGEAITRGGDDRPNKNI 76 Query: 50 GTGSVVER-DGVFHFYYTGFNENNRDTEGKHEQVLMRAISDDLIQWRKDPAFFVPPYTER 108 TGSV+++ + ++ +YT FN + + GK Q +MRA S DLI W D F Sbjct: 77 YTGSVIKKGENEYYAFYTAFNADIQ-IHGKSVQSVMRAFSTDLIHWETDEDFLFVADDSM 135 Query: 109 YGGLHWRDPQVFWNDQAGEYWMAVTTTEKDGPMNRSGCTHIMRSADLEQWKHGEMIYAPR 168 Y WRDP VF N++ G YWM + +K G +R GCT + +S DL W + E YAP Sbjct: 136 YESFDWRDPYVFKNEEEGNYWMLLAARKKGGGAHRGGCTALCKSTDLVNWTYEEPFYAPE 195 Query: 169 SYATHECHDIFQMGGWWYMVFSNYTRWWETRYRMAKSPEGPWFIPPYDDMFDGRGFYAAK 228 Y T EC ++FQMGGWWY+VFS ++ + T YRMA++ +GPW IP DD+FD R YA K Sbjct: 196 MYVTMECPEVFQMGGWWYLVFSTFSDRFTTHYRMARTLDGPWGIPD-DDVFDTRADYAIK 254 Query: 229 TVSNGTNRYLVGWQAIRSNMDDMSPYQWGGSTLVHELVQHPDGTLGVQLIPAIQDSFRQE 288 T +G R+ GW A ++ +D P++WGG+ + HE+VQ PDGTL V+ A++D + Q Sbjct: 255 TAFDGQKRFAFGWIASKAGNNDYGPWEWGGTMVAHEIVQEPDGTLSVKPTEAMKDFYNQV 314 Query: 289 LPQAPAA--HLGVWDSDTAAITGKEPYGFGWTELGRMAGDCLIEASVQWSSDTQACGIML 346 LP H + I E G + + + + S++T G+ L Sbjct: 315 LPVNDLCYYHCTAREEAGKTIIQSETLGAVLFPVPEQGFELDLTITPGQSAET---GVAL 371 Query: 347 HTSGAGLEKWCQVRLEPQRNRLIFD---RSN------KFDGHHLFVEERYLPLDGSSGAD 397 HT G+E +R++ R +D RS + G E L + Sbjct: 372 HTD-TGMENGYFLRMDLSRRTAAWDMWPRSGQGVYQWQIKGDIPCQIETSRKLPAADTYH 430 Query: 398 IKIVASGNIIIIYVNN-TALATRAYNYEPGSFGLFVEHGEAAFSNVKIS 445 I+I ++ ++YVN+ A++TR Y+++ G G++ G AA S +I Sbjct: 431 IRIFREDDLCVLYVNDRMAMSTRMYDHKGGLAGIYTVGGTAAVSGCQIK 479 >A1R3C5.1 A1R3C5_ARTAT Putative glycosyl hydrolases family 32 protein Length = 478 Score = 248 bits (632), Expect = 1e-63, Method: Compositional matrix adjust. Identities = 160/472 (33%), Positives = 237/472 (50%), Gaps = 41/472 (8%) Query: 1 MPFYHEGVYHLYYLHK--------YCIYEVATKDFVSYGEPRLAVVCGSPEEQDWHIGTG 52 +PF +G + L+YLH+ V TKD + + +++ GS + D++ TG Sbjct: 18 IPFEKDGEFWLFYLHEDRSDPKPGTSWNLVTTKDLTQFEDHGVSLSHGSESDLDFNAYTG 77 Query: 53 SVV-ERDGVFHFYYTGFNENNRDTEGKHEQVLMRAISDD-LIQWRKDPAF-FVPPYTERY 109 SV+ + GV H +YTG N N +G Q++M A S D + W+K P F P + Y Sbjct: 78 SVLADAAGVHHLFYTGQNPRNVGPDGMPRQLVMHATSTDGMRTWQKHPELTFGAP--DGY 135 Query: 110 GGLHWRDPQVFWNDQAGEYWMAVTTTEKDGPMNRSGCTHIMRSADLEQWKHGEMIYAPRS 169 WRDP VFW+D ++ M + +GP R G S DL W+H E + PR Sbjct: 136 ESGDWRDPFVFWDDSKNQWRMLLAARHSEGPERRRGVIAQCVSTDLMTWQHTEPFWDPRR 195 Query: 170 YATHECHDIFQMGGWWYMVFSNYTRWWETRYRMAKSPEGPWFIPPYDDMFDGRGFYAAKT 229 Y THEC D+F G WWYMV+S ++ + TRYRMAKSP+GPW +P D + DGR FYA+KT Sbjct: 196 YITHECPDVFSWGDWWYMVYSEFSESFTTRYRMAKSPDGPWTVPALDSI-DGRAFYASKT 254 Query: 230 VSNGTNRYLVGWQAIRSNMDDMSPYQWGGSTLVHELVQHPDGTLGV----QLIPAIQDSF 285 R+ GW A + D +QW G+ V E Q+PDGTLG +L+ + D Sbjct: 255 AERDGRRFFFGWIASKEGNADQGAWQWAGTMSVLEARQNPDGTLGFSFADELVESFWDDV 314 Query: 286 RQELPQAPAAHLGVWDSDTAAITGKEPYGFGWTELGRMAGDCLIEASVQWSSDTQACGIM 345 L + A L V D TA ++ +E + +A + + DT CG++ Sbjct: 315 SVPLTGSLPARLHVPDGYTALVSDEE-----------LPQQFYAKAVLDIAPDTTECGLL 363 Query: 346 LHTSGAGLEKWCQVRLEPQRNRLIFDRSNK---------FDGHHLFVEERYLPLDGSSGA 396 L +S G + +RLEP+R RL+FDR + G F E P D + G Sbjct: 364 LRSSHDGDHSYV-LRLEPKRGRLVFDRWPRTITGDAQWHVSGDVPFDVELERPCDLTPGE 422 Query: 397 -DIKIVASGNIIIIYVN-NTALATRAYNYEPGSFGLFVEHGEAAFSNVKISE 446 ++++ G+I + V+ AL+TR Y+ G GLF G A + +++ + Sbjct: 423 HTLEVIVDGDICVAVVDRQVALSTRIYDLTAGRIGLFAGEGSATVTELEVRQ 474 >C4GDD6.1 C4GDD6_9FIRM Putative uncharacterized protein Length = 548 Score = 239 bits (609), Expect = 5e-61, Method: Compositional matrix adjust. Identities = 161/487 (33%), Positives = 236/487 (48%), Gaps = 47/487 (9%) Query: 1 MPFYHEGVYHLYYLH-------------KYCIYEVATKDFVSYGEPRLAVVCGSPEEQDW 47 +PF H+G ++++YLH + + T+D V + + + + G E+ D+ Sbjct: 58 IPFAHDGKFYIFYLHDERKGHSEDEYGYRTSWNLLITEDGVHFEDKGIVLPVGGYEDADY 117 Query: 48 HIGTGSVVE-RDGVFHFYYTGFNENN--RDTEGKHEQVLMRAISDDLIQWRKDPAFFVPP 104 TGSV+ +DG FH +YT N N +G+ Q + AIS+DLI W KDP+ Sbjct: 118 ACYTGSVIAGKDGRFHLFYTAQNNYNPIYHRDGRPLQFVAHAISEDLIHWEKDPSAIFGA 177 Query: 105 YTERYGGLHWRDPQVFWNDQAGEYWMAVTTTEKDGPMNRSGCTHIMRSADLEQWKHGEMI 164 Y WRDP VF+N + Y M + K GC + S DL W+ E Sbjct: 178 DESIYEPFDWRDPFVFYNGEEDCYNMLLAARLKGAGDKNGGCVGLCHSKDLIHWQAAEPF 237 Query: 165 YAPRSYATHECHDIFQMGGWWYMVFSNYTRWWETRYRMAKSPEGPWFIPPYDDMFDGRGF 224 Y P SY THEC D+F+MGG WY+V+S ++ + T YR+A S GPW P +D FDGR F Sbjct: 238 YHPESYMTHECPDLFRMGGKWYLVYSTFSEKFVTHYRVADSLGGPW-TAPVEDTFDGRAF 296 Query: 225 YAAKTVSNGTNRYLVGWQAIRSNMDDMSPYQWGGSTLVHELVQHPDGTLGVQLIPAIQDS 284 YAAKT R+ W R D Y+WGG+ L+H+L Q DG L V A++++ Sbjct: 297 YAAKTAETKGRRWAFAWVPTRRGGSDFGQYEWGGTLLMHQLKQEADGRLSVSPPRAVREA 356 Query: 285 FRQELPQA-PAAHLGVWDSDTAAITGKEPYGFGWTELGRMAG-----------DCLIEAS 332 F L P G +++ G+ G EL + G DC+IEA Sbjct: 357 FEARLILCGPEPEEGGKETED---KGEYRRGGSKLELRNIQGKKNLIFDGMEEDCMIEAD 413 Query: 333 VQWSSDTQACGIMLHTSGAGLEKWCQVRLEPQRNRLIFDRSNK---------FDGHHLFV 383 + + T+A G+ + +EK RLEP NRL+ D + DG F+ Sbjct: 414 LIYEEGTRAFGLAVRQD-EKMEKGYYFRLEPFYNRLVADMWPRRIAGVNQWYIDGDKAFL 472 Query: 384 EERYLPLDGSSGADIK----IVASGNIIIIYVNNT-ALATRAYNYEPGSFGLFVEHGEAA 438 E PLD S + + ++A G+I+ +YVN++ AL RAY +G FV+ G Sbjct: 473 VELERPLDFSKLQNRRLHLCLIAEGSILCLYVNDSLALTMRAYESGRSHWGFFVDDGGIQ 532 Query: 439 FSNVKIS 445 NV+++ Sbjct: 533 IENVRLT 539 >C2KX06.1 C2KX06_9FIRM Possible beta-fructofuranosidase Length = 482 Score = 224 bits (572), Expect = 1e-56, Method: Compositional matrix adjust. Identities = 149/476 (31%), Positives = 225/476 (47%), Gaps = 42/476 (8%) Query: 1 MPFYHEGVYHLYYLH-------------KYCIYEVATKDFVSYGEPRLAVVCGSPEEQDW 47 +PF H+G ++++YLH + + T+D V+ + ++ + G ++ D+ Sbjct: 17 IPFAHDGKFYIFYLHDERKGNTEDEYGYRTSWNLLITEDGVNIKDCKVVLPVGGYDDADY 76 Query: 48 HIGTGSVVE-RDGVFHFYYTGFNENNRD--TEGKHEQVLMRAISDDLIQWRKDPAFFVPP 104 TGSV+E D FH +YT N N G+ Q + AIS+DLI W K P Sbjct: 77 ACYTGSVIEGNDSKFHMFYTAQNNYNPKYHRNGRPLQYVAHAISEDLIHWEKLPELTFGA 136 Query: 105 YTERYGGLHWRDPQVFWNDQAGEYWMAVTTTEKDGPMNRSGCTHIMRSADLEQWKHGEMI 164 Y WRDP VF+N++ + M + + GC + RS DL WK + Sbjct: 137 DESIYEPFDWRDPFVFYNEEEECFDMLLAARLQGASEKNGGCVGLCRSYDLIHWKAMQPF 196 Query: 165 YAPRSYATHECHDIFQMGGWWYMVFSNYTRWWETRYRMAKSPEGPWFIPPYDDMFDGRGF 224 Y P+SY THEC D+F+MG WY++FS ++ + T YRMA GPW P +D FD R F Sbjct: 197 YNPQSYMTHECPDLFKMGDKWYLIFSTFSEKFVTHYRMADKISGPW-TAPIEDTFDARAF 255 Query: 225 YAAKTVSNGTNRYLVGWQAIRSNMDDMSPYQWGGSTLVHELVQHPDGTLGVQLIPAIQDS 284 YAAKT R W + D Y+WGG+ + HE+ Q DG L V+ + + Sbjct: 256 YAAKTAQAKDVRMAFAWVPTKRGDSDFGQYEWGGNLIAHEIKQDKDGKLKVKPSNGLINI 315 Query: 285 FRQELPQAPAAHLGVWDSDTAAITGKEPYGFGWTELGRMAGDCLIEASVQWSSDTQACGI 344 F +E+ + D + G++ Y + M CLIEA++++ ++A GI Sbjct: 316 FEKEITAN-----NIEDVEIKNNEGEKVY-----VIEGMKETCLIEATIEFYEGSRAFGI 365 Query: 345 MLHTSGAGLEKWCQVRLEPQRNRLIFDRSNK---------FDGHHLFVEERYLPLD---- 391 L +R EP NR++ D + DG F+ E P D Sbjct: 366 GLRQD-KDFANGYYLRFEPFYNRIVADMWPRRISGVNQWYVDGDKPFMVELERPFDYKAL 424 Query: 392 GSSGADIKIVASGNIIIIYVNNT-ALATRAYNYEPGSFGLFVEHGEAAFSNVKISE 446 + +IK+ G+II +YVN+T AL RAY+ E +G FV+ G N+ + E Sbjct: 425 EENKVNIKVFVEGSIICLYVNDTVALTMRAYSTERKYWGFFVKDGSIRVCNIHMYE 480 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.137 0.457 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,113,096,714 Number of extensions: 143170403 Number of successful extensions: 251604 Number of sequences better than 1.0e-05: 112 Number of HSP's gapped: 251541 Number of HSP's successfully gapped: 112 Length of query: 446 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 307 Effective length of database: 1,751,175,118 Effective search space: 537610761226 Effective search space used: 537610761226 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)