BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0571.0004.p (373 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KHI2.1 B3KHI2_9BACI Putative uncharacterized protein 383 e-104 B1DBM1.1 B1DBM1_9BACL YkvI 329 3e-88 Q2B5H3.1 Q2B5H3_9BACI YkvI 231 1e-58 B7GLA9.1 B7GLA9_ANOFW Uncharacterized membrane protein 223 3e-56 O31674.1 YKVI_BACSU Uncharacterized membrane protein ykvI 221 1e-55 >B3KHI2.1 B3KHI2_9BACI Putative uncharacterized protein Length = 348 Score = 383 bits (983), Expect = e-104, Method: Compositional matrix adjust. Identities = 193/335 (57%), Positives = 239/335 (71%) Query: 1 MKSHVRTLQIAFTYIGTIVGAGFATGQEILRFFTRYGHWAVLTILFSTVLFIWLGTKMMI 60 M++ +R LQIAFTYIGT+VGAGFATG+EIL+FFT+YGHWA LTI+ +T LFIWLGTK+M Sbjct: 1 MRNFMRVLQIAFTYIGTVVGAGFATGREILQFFTQYGHWATLTIVLTTGLFIWLGTKIMT 60 Query: 61 IARRVQAESYEDFNRHLFGERAGRSISLFTMIILVGVNSIMLAGAGAIFQEHLGMPYQAG 120 I+RR+ A+S+ED N+ LFGE+AG ISLF +IL+GVNSIMLAGAG++F EHL + YQ G Sbjct: 61 ISRRIGAKSFEDLNKFLFGEKAGVWISLFMFVILLGVNSIMLAGAGSVFVEHLNLSYQTG 120 Query: 121 XXXXXXXXXXXXKRGISGILQINSLVVPLMLTLSLIIIFNTLGVPGAERFLFLPTDHSFF 180 ++GI I+ +NSLVVP+MLTLS+ +I TL PGA RFL L TDHS Sbjct: 121 LFVTMIGTYFILRKGIQSIMTMNSLVVPMMLTLSVAVISQTLQSPGATRFLTLTTDHSLP 180 Query: 181 GAWMSPLLYTAFNLGMAQAVLVPLARHTNDEQAXXXXXXXXXXXXXXXXXAAHFAMSSQM 240 W++PLLYTAFNL MA VLVPL T + AAHFAMS+ M Sbjct: 181 SVWLAPLLYTAFNLVMAMPVLVPLGSQTESARTVTWGGIIGGTGVGFMLMAAHFAMSAHM 240 Query: 241 PGILQYEIPMGTIAIRLGPAVQSIYLLLIFMEIFSTFVADIYGVSIQLQQRXXXXXXXXX 300 PGI QYEIPMG+IA +LG VQ IYL+LIF+EIFSTFVA+IYG+++Q+QQR Sbjct: 241 PGISQYEIPMGSIANQLGWFVQIIYLILIFLEIFSTFVANIYGITLQVQQRTSLSPRVIT 300 Query: 301 XXXXXICYVFSQFGFSSLLSIFYPIFGAVSLVWVI 335 CY FSQFGFSSLLS+ YP+FG + L+WV+ Sbjct: 301 LGIMVFCYFFSQFGFSSLLSLLYPMFGGLCLLWVL 335 >B1DBM1.1 B1DBM1_9BACL YkvI Length = 345 Score = 329 bits (843), Expect = 3e-88, Method: Compositional matrix adjust. Identities = 166/334 (49%), Positives = 214/334 (64%) Query: 1 MKSHVRTLQIAFTYIGTIVGAGFATGQEILRFFTRYGHWAVLTILFSTVLFIWLGTKMMI 60 +K + LQIA TYIGTIVGAGFATGQEIL+FFTR+G+W LTI +T LFIWLG KMM+ Sbjct: 2 VKRTGKVLQIASTYIGTIVGAGFATGQEILQFFTRFGYWGTLTIAVATALFIWLGGKMML 61 Query: 61 IARRVQAESYEDFNRHLFGERAGRSISLFTMIILVGVNSIMLAGAGAIFQEHLGMPYQAG 120 IA + A+SYED N LFG++ G+ IS F +++L+GV ++MLAGAG IF E+ + YQ G Sbjct: 62 IASEIGAKSYEDLNIALFGDKYGKWISHFMLVVLLGVTAVMLAGAGTIFYENWNVSYQTG 121 Query: 121 XXXXXXXXXXXXKRGISGILQINSLVVPLMLTLSLIIIFNTLGVPGAERFLFLPTDHSFF 180 ++G+ IL +N++VVP+ML + +I+ +T P A+RF+ + TDHS + Sbjct: 122 LLVTIAACYFLLRKGMKAILTVNAIVVPIMLLFTALIMLDTFQTPEADRFITIATDHSVW 181 Query: 181 GAWMSPLLYTAFNLGMAQAVLVPLARHTNDEQAXXXXXXXXXXXXXXXXXAAHFAMSSQM 240 AW SP LYTAFNL MAQAVLVPL D + A H A+S M Sbjct: 182 AAWASPFLYTAFNLSMAQAVLVPLGSEVQDRKTIVLGSWIGGIGIGIMLLAGHIALSVHM 241 Query: 241 PGILQYEIPMGTIAIRLGPAVQSIYLLLIFMEIFSTFVADIYGVSIQLQQRXXXXXXXXX 300 PGI Q+ IPMG IA +LG VQ IY+ LIF EIF+T +ADIYG+++QLQ+R Sbjct: 242 PGIQQFAIPMGGIAKQLGHIVQYIYIFLIFAEIFTTLIADIYGLTLQLQERLKWSRPLIT 301 Query: 301 XXXXXICYVFSQFGFSSLLSIFYPIFGAVSLVWV 334 CY SQ GF LLS YPIFG +SL W+ Sbjct: 302 LGILAFCYFASQIGFGPLLSTLYPIFGLISLGWL 335 >Q2B5H3.1 Q2B5H3_9BACI YkvI Length = 347 Score = 231 bits (588), Expect = 1e-58, Method: Compositional matrix adjust. Identities = 126/336 (37%), Positives = 197/336 (58%), Gaps = 2/336 (0%) Query: 1 MKSHVRTLQIAFTYIGTIVGAGFATGQEILRFFTRYGHWAVLTILFSTVLFIWLGTKMMI 60 MK+ LQIA Y+GT+VGAGFATG+EI+ FFTR+G ++ IL S +FI LGTK+M Sbjct: 1 MKNWTGALQIAAVYVGTVVGAGFATGKEIVEFFTRFGFIGLIGILMSGYIFIMLGTKLMR 60 Query: 61 IARRVQAESYEDFNRHLFGERAGRSISLFTMIILVGVNSIMLAGAGAIFQEHLGMPYQAG 120 I+ R+ A+SY++ N +LFG G+ I++ T+I+L+GV ++ML+GAGA+FQE LG+ +G Sbjct: 61 ISARIHAKSYQELNDYLFGRYFGKVINVLTLIMLLGVCAVMLSGAGAVFQEQLGLSKLSG 120 Query: 121 XXXXXXXXXXXXKRGISGILQINSLVVPLMLTLSLIIIFNTLGVPG-AERFLFLPTDHSF 179 GI G+ +N VVP+M+ S I++F ++ +PG E+ LF+P Sbjct: 121 VLATIILSVVVMILGIKGLFAVNLFVVPMMIAFSFILMFISIKLPGFKEQMLFIPFAEDG 180 Query: 180 FGAWMSPLLYTAFNLGMAQAVLVPLARHTNDEQAXXXXXXXXXXXXXXXXXAAHFAMSSQ 239 + A +P YTA NL +AQAVLVP+A D++ ++H + Sbjct: 181 WKAVAAPFSYTALNLALAQAVLVPVAAEIKDDRTIRMGGYIGGAALTIILLSSHLTL-VM 239 Query: 240 MPGILQYEIPMGTIAIRLGPAVQSIYLLLIFMEIFSTFVADIYGVSIQLQQRXXXXXXXX 299 +P + YEIPM I L +++L+I+ EIF++ + +I+G+ Q++Q Sbjct: 240 LPNLEVYEIPMAVIMKNLASGFYGLFVLVIYGEIFTSIIGNIFGLERQIRQYISIPAIWS 299 Query: 300 XXXXXXICYVFSQFGFSSLLSIFYPIFGAVSLVWVI 335 I + S +S+LLS YPIFG VS+++++ Sbjct: 300 VGGIYLITCLISMIEYSTLLSYLYPIFGYVSILFIV 335 >B7GLA9.1 B7GLA9_ANOFW Uncharacterized membrane protein Length = 331 Score = 223 bits (567), Expect = 3e-56, Method: Compositional matrix adjust. Identities = 129/330 (39%), Positives = 192/330 (58%), Gaps = 13/330 (3%) Query: 6 RTLQIAFTYIGTIVGAGFATGQEILRFFTRYGHWAVLTILFSTVLFIWLGTKMMIIARRV 65 R +QIAF Y+GT+VGAGFATG+EI++FFT+YG V+TI S LFI+ GT++M++A R+ Sbjct: 4 RAVQIAFVYVGTVVGAGFATGKEIVQFFTQYGSIGVVTIAMSGCLFIFFGTRLMVMANRL 63 Query: 66 QAESYEDFNRHLFGERAGRSISLFTMIILVGVNSIMLAGAGAIFQEHLGMPYQAGXXXXX 125 QA SYE FN +LFG + ++LF ++L V S+ML+GAGA+F+E L MP G Sbjct: 64 QARSYEQFNAYLFG-KMSVVVNLFMFVVLFSVTSVMLSGAGAVFEEQLHMPAYVGLLFTM 122 Query: 126 XXXXXXXKRGISGILQINSLVVPLMLTLSL-IIIFNTLGVPGAERFLFLPTDHSFFGAWM 184 G++GIL +N +VVPLM+ SL I + N + + P +FF Sbjct: 123 LLAFVTMLFGLNGILSVNMVVVPLMIIFSLSIALINESDIVWTK-----PPLAAFF---- 173 Query: 185 SPLLYTAFNLGMAQAVLVPLARHTNDEQAXXXXXXXXXXXXXXXXXAAHFAMSSQMPGIL 244 SP Y AFNL MAQ VLVPLA DE+A ++HFA+ S +P + Sbjct: 174 SPFAYVAFNLAMAQVVLVPLATEVRDERAIRLGGWLGGILLMFILFSSHFAL-SLLPNLP 232 Query: 245 QYEIPMGTIAIRLGPAVQSIYLLLIFMEIFSTFVADIYGVSIQLQQRXXXXXXXXXXXXX 304 Y IPM + R + +Y+++I+ EIF++F+A ++G+ +L Sbjct: 233 SYSIPMAEVVKRSLTSFYGLYVVMIYGEIFTSFIAGVFGLHRKLALFSPTPTFVLVMLLF 292 Query: 305 XICYVFSQFGFSSLLSIFYPIFGAVSLVWV 334 + Y FS G+ +LL++ YP+FG VS++++ Sbjct: 293 AM-YAFSLVGYHALLTVLYPLFGYVSIIFI 321 >O31674.1 YKVI_BACSU Uncharacterized membrane protein ykvI Length = 347 Score = 221 bits (562), Expect = 1e-55, Method: Compositional matrix adjust. Identities = 122/333 (36%), Positives = 188/333 (56%), Gaps = 16/333 (4%) Query: 9 QIAFTYIGTIVGAGFATGQEILRFFTRYGHWAVLTILFSTVLFIWLGTKMMIIARRVQAE 68 Q+AF Y+GT+VGAGFATG+EI+ FF ++G + IL S +F LG K+MII++R+ A+ Sbjct: 10 QLAFVYVGTVVGAGFATGREIVEFFLKFGWFGFFGILVSGGMFTLLGAKLMIISKRINAK 69 Query: 69 SYEDFNRHLFGERAGRSISLFTMIILVGVNSIMLAGAGAIFQEHLGMPYQAGXXXXXXXX 128 SY++ N LFG AGR I++F + +L+GV S+ML+GAGA+F+E LGM Q G Sbjct: 70 SYQEMNIFLFGATAGRIINVFMLFVLLGVTSVMLSGAGALFEEQLGMSAQIGMLITIGLS 129 Query: 129 XXXXKRGISGILQINSLVVPLMLTLSLIIIFNTLGVPGAERFLFLPTDHSFFGAW----- 183 RG+ GI +N VVPL++ S+I++ A+ F+F + ++ W Sbjct: 130 LIVMTRGVKGIFGVNVFVVPLLIIFSMIVV--------ADSFIFSESRNAAQWVWPHRWD 181 Query: 184 --MSPLLYTAFNLGMAQAVLVPLARHTNDEQAXXXXXXXXXXXXXXXXXAAHFAMSSQMP 241 +S + Y A NL +AQAVLVPLA + E+ A+ ++S+ +P Sbjct: 182 WLLSAVSYGALNLSLAQAVLVPLANEMSSEKVIKKGALIGGTMLTIVLSASFLSLST-LP 240 Query: 242 GILQYEIPMGTIAIRLGPAVQSIYLLLIFMEIFSTFVADIYGVSIQLQQRXXXXXXXXXX 301 + Y+IPM + +V IYLL+IF E+F++ + ++YG+ Q+Q Sbjct: 241 DVFLYDIPMAQVVYLFARSVHLIYLLIIFGEVFTSVIGNLYGLEKQVQSFLPVKSKYIFA 300 Query: 302 XXXXICYVFSQFGFSSLLSIFYPIFGAVSLVWV 334 Y+ SQ G+ L+S YP+FG VSL ++ Sbjct: 301 AIMITAYITSQIGYGRLISTIYPLFGYVSLAFI 333 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.331 0.143 0.429 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,645,667,238 Number of extensions: 54769792 Number of successful extensions: 203105 Number of sequences better than 1.0e-05: 295 Number of HSP's gapped: 202497 Number of HSP's successfully gapped: 295 Length of query: 373 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 236 Effective length of database: 1,770,017,148 Effective search space: 417724046928 Effective search space used: 417724046928 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 132 (55.5 bits)