BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0569.0005.p (275 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C3RVP9.1 C3RVP9_9CYAN SxtU 146 2e-33 B3EYH3.1 B3EYH3_9NOST Short-chain alcohol dehydrogenase 145 4e-33 C0UA94.1 C0UA94_9ACTO Short-chain alcohol dehydrogenase 144 1e-32 A2U0T4.1 A2U0T4_9FLAO Short chain dehydrogenase 143 2e-32 B9LVR4.1 B9LVR4_HALLT Short-chain dehydrogenase/reductase SDR 142 3e-32 >C3RVP9.1 C3RVP9_9CYAN SxtU Length = 249 Score = 146 bits (369), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 95/263 (36%), Positives = 144/263 (54%), Gaps = 27/263 (10%) Query: 2 LNHKTAVITGASSGIGRAIATRLAAQGVHVFITGRSREQLEEVKSQIELAGGQATCSAFD 61 L+ K A+ITGASSGIG A A LAA+G V I R ++L+ + +IE +GGQA D Sbjct: 5 LDGKVAIITGASSGIGEATAFALAAEGAKVAIAARRADRLDGLAKRIEASGGQALPIVTD 64 Query: 62 LRDTGRLQDFIRQTRTQTGHLDIMVNNAGLHIHGNIVDTKVEDWREMIDVNIVALLAGSQ 121 + D ++ +++T+ + GH+DI+VNNAG+ + G I DWR DVN++ +L Sbjct: 65 ITDEAQVNHLVQKTKVELGHVDILVNNAGIGVFGTIDTGNPADWRRAFDVNVLGVLYAIH 124 Query: 122 AAVQAMRLNEGGAPGHIINISS--GNAYEPGRDFYSATKHMVNAIGQALTRELAQEPIQI 179 A + + + GHI+NISS G + G YSATK VNA+ +AL +E+ ++ I++ Sbjct: 125 AVLPLFKAQKS---GHIVNISSVDGRIAQAGAVVYSATKSGVNALSEALRQEVYKDNIRV 181 Query: 180 TTILPGAVXXXXGRNLEPEAVVQLARKLGMEVEYKQGDHLPEELIARISGAMGQTFLSGD 239 T I PG V +L + + + L EE + I T L + Sbjct: 182 TIIEPGLVDTPF-IDLISDPITK---------------QLSEEELKTI------TPLQSE 219 Query: 240 DIARAVVYALQQPQNVVVRELYL 262 DIARA++YA+ QP+ V V E+ + Sbjct: 220 DIARAIIYAVTQPEYVNVNEILI 242 >B3EYH3.1 B3EYH3_9NOST Short-chain alcohol dehydrogenase Length = 249 Score = 145 bits (366), Expect = 4e-33, Method: Compositional matrix adjust. Identities = 94/263 (35%), Positives = 141/263 (53%), Gaps = 27/263 (10%) Query: 2 LNHKTAVITGASSGIGRAIATRLAAQGVHVFITGRSREQLEEVKSQIELAGGQATCSAFD 61 L+ K A+ITGASSGIG A A LAA+G V I R E L + +IE +GGQA D Sbjct: 5 LDGKVAIITGASSGIGEATAFALAAEGAKVAIAARRAELLHALAKRIEASGGQALPIVTD 64 Query: 62 LRDTGRLQDFIRQTRTQTGHLDIMVNNAGLHIHGNIVDTKVEDWREMIDVNIVALLAGSQ 121 + D ++ +++T+ + GH+DI+VNNAG+ + G I DWR DVN++ +L Sbjct: 65 ITDESQVNHLVQKTKVELGHVDILVNNAGIGVFGAIDTGNPADWRRAFDVNVLGVLYAIH 124 Query: 122 AAVQAMRLNEGGAPGHIINISS--GNAYEPGRDFYSATKHMVNAIGQALTRELAQEPIQI 179 A + ++ + GHI+NISS G + G YSA K VNA+ +AL +E++ + I++ Sbjct: 125 AVLPLLKAQKS---GHIVNISSVDGRIAQSGAVVYSAAKSGVNALSEALRQEVSLDNIRV 181 Query: 180 TTILPGAVXXXXGRNLEPEAVVQLARKLGMEVEYKQGDHLPEELIARISGAMGQTFLSGD 239 T I PG V + QL++ E ++ I T L + Sbjct: 182 TIIEPGLVDTPFNDLISDPITKQLSK----------------EQLSTI------TPLQSE 219 Query: 240 DIARAVVYALQQPQNVVVRELYL 262 DIARA++YA+ QP +V V E+ + Sbjct: 220 DIARAIIYAVTQPDHVNVNEILI 242 >C0UA94.1 C0UA94_9ACTO Short-chain alcohol dehydrogenase Length = 252 Score = 144 bits (362), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 89/263 (33%), Positives = 140/263 (53%), Gaps = 27/263 (10%) Query: 2 LNHKTAVITGASSGIGRAIATRLAAQGVHVFITGRSREQLEEVKSQIELAGGQATCSAFD 61 L+ K A++TGASSGIG A A L G V I R R++L+++ +++ AGG+ D Sbjct: 8 LSGKVALVTGASSGIGEATAVALVEAGAAVAIGARRRDRLDDLAARLSDAGGKVVPLDLD 67 Query: 62 LRDTGRLQDFIRQTRTQTGHLDIMVNNAGLHIHGNIVDTKVEDWREMIDVNIVALLAGSQ 121 + D +D + +TR + LD++VNNAG+ + G IV EDWR M+D N++ ++ + Sbjct: 68 VTDEASCRDAVARTRAKLCGLDVLVNNAGVMLLGTIVGADPEDWRRMLDTNVLGVMYPTH 127 Query: 122 AAVQAMRLNEGGAPGHIINISS--GNAYEPGRDFYSATKHMVNAIGQALTRELAQEPIQI 179 AA+ M L +G G I+NISS G G Y+A+K VNA ++L +E+ ++I Sbjct: 128 AAIDGM-LEQGS--GDIVNISSTAGRTARAGAGVYNASKWAVNAFSESLRQEVTARGVRI 184 Query: 180 TTILPGAVXXXXGRNLEPEAVVQLARKLGMEVEYKQGDHLPEELIARISGAMGQTFLSGD 239 + + PGAV E E + PE A ++ + G L + Sbjct: 185 SLVEPGAV----------------------ETELRSHITQPEAKAAALAHSEGMRSLRPE 222 Query: 240 DIARAVVYALQQPQNVVVRELYL 262 D+ARA+VY + QP +V V E+ + Sbjct: 223 DVARAIVYVVTQPPHVAVNEVLV 245 >A2U0T4.1 A2U0T4_9FLAO Short chain dehydrogenase Length = 257 Score = 143 bits (360), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 92/264 (34%), Positives = 135/264 (51%), Gaps = 29/264 (10%) Query: 2 LNHKTAVITGASSGIGRAIATRLAAQGVHVFITGRSREQLEEVKSQIELAGGQATCSAFD 61 L K A+ITGASSGIG A A +LA +G V +T RS ++L+E+ +IE G A D Sbjct: 14 LKDKVAIITGASSGIGEATALKLAKEGAKVVLTARSEDKLKELAQKIENENGTALVVTAD 73 Query: 62 LRDTGRLQDFIRQTRTQTGHLDIMVNNAGLHIHGNIVDTKVEDWREMIDVNIVALLAGSQ 121 + + ++ + + +++ G ++I+VNNAGL I K E+W M+DVNI +L G Q Sbjct: 74 VTKSDEFKNVVEKAKSEFGTVNILVNNAGLMPLSYIEKFKTEEWNTMVDVNIKGVLNGVQ 133 Query: 122 AAVQAMRLNEGGAPGHIINISSGNAYE--PGRDFYSATKHMVNAIGQALTREL-AQEPIQ 178 A + + N+G GHIINISS AY+ PG Y ATK V + L +E+ A+ I Sbjct: 134 AVLPTLIDNKG---GHIINISSTAAYKYFPGGAVYCATKAAVQMFSEGLRKEISAKHGIN 190 Query: 179 ITTILPGAVXXXXGRNLEPEAVVQLARKLGMEVEYKQGDHLPEELIARISGAMGQTFLSG 238 +T+I PGAV + E + EEL T L Sbjct: 191 VTSIEPGAVDTALFETITDE-------------------DIKEEL----KDMKKMTTLQA 227 Query: 239 DDIARAVVYALQQPQNVVVRELYL 262 +DIA A+ YA+ QP + +++ Sbjct: 228 EDIANAIFYAVNQPSRANINNIHI 251 >B9LVR4.1 B9LVR4_HALLT Short-chain dehydrogenase/reductase SDR Length = 259 Score = 142 bits (358), Expect = 3e-32, Method: Compositional matrix adjust. Identities = 96/269 (35%), Positives = 144/269 (53%), Gaps = 31/269 (11%) Query: 2 LNHKTAVITGASSGIGRAIATRLAAQGVHVFITGRSREQLEEVKSQIELAGGQATCSAFD 61 L+ + A++TGASSGIGRA+AT LAA G V + R E+LE++ +IE GG A D Sbjct: 16 LDGEVALVTGASSGIGRAVATTLAADGAAVAVAARREERLEDLVDEIETDGGTALAVPTD 75 Query: 62 LRDTGRLQDFIRQTRTQTGHLDIMVNNAGLHIHGNIVDTKVEDWREMIDVNIVALLAGSQ 121 + DT + + I TR + G LDI++NNAG+ + ++ + +D ++M+DVN+ L+A ++ Sbjct: 76 VTDTDAVHEMIATTRAELGGLDILINNAGVMLLAPVIRAEHDDLQQMLDVNLKGLMAATR 135 Query: 122 AAVQAMRLNEGGAPGHIINISS--GNAYEPGRDFYSATKHMVNAIGQALTRELAQEPIQI 179 A+ + L++ GHI+NISS G YSATK VNA ++L +E+A +++ Sbjct: 136 EALPGL-LDQNS--GHIVNISSVAGQTANETSGGYSATKFGVNAFSESLRKEIADSDVRV 192 Query: 180 TTILPGAVXXXXGRNLEPEAVVQLARKLGMEVEYKQGDHLPEELIARISGAMGQTF--LS 237 T + PGA VE + GDH+P+E + L Sbjct: 193 TVVSPGA------------------------VETELGDHIPDEQTKERMADLTDDLIPLH 228 Query: 238 GDDIARAVVYALQQPQNVVVRELYLTAPN 266 DDIA + YAL QP V V EL + N Sbjct: 229 PDDIANGIAYALTQPPRVSVNELVIRPTN 257 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.134 0.376 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,531,027,793 Number of extensions: 58921785 Number of successful extensions: 258621 Number of sequences better than 1.0e-05: 28093 Number of HSP's gapped: 228147 Number of HSP's successfully gapped: 28140 Length of query: 275 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 141 Effective length of database: 1,798,280,193 Effective search space: 253557507213 Effective search space used: 253557507213 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)