BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0569.0001.p (327 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C2D062.1 C2D062_LACBR Amidohydrolase 2 282 3e-74 C1R506.1 C1R506_9ACTO Predicted TIM-barrel fold metal-dependent ... 273 1e-71 C2FLY3.1 C2FLY3_LACPL Amidohydrolase 2 265 4e-69 Q88TJ2.1 Q88TJ2_LACPL Metal-dependent hydrolase (Putative) 263 1e-68 C2FAS4.1 C2FAS4_LACPA Amidohydrolase 2 240 1e-61 >C2D062.1 C2D062_LACBR Amidohydrolase 2 Length = 311 Score = 282 bits (722), Expect = 3e-74, Method: Compositional matrix adjust. Identities = 145/314 (46%), Positives = 193/314 (61%), Gaps = 12/314 (3%) Query: 12 KNDLHSHYLPSAYREALLKHAGPNPDGFPTPVWHADLHLEAMEQLGIAVSLLSLSSPHIH 71 K DLH+HYLP Y AL H +PDG+PTP W L M+Q GI S+LSLSSPHI+ Sbjct: 5 KIDLHTHYLPKGYVAALKTHIAGDPDGWPTPEWQVGTTLNFMKQNGINYSVLSLSSPHIN 64 Query: 72 WGDREAARELARRVNEDGAELVRKYPGKFGLLASLPVPEISDSIVEIKYAMDVLHADGFT 131 + D+ LA N+ G +L KYP + G LA+LP P ++S+ + A D HA G T Sbjct: 65 FNDKAETIRLAEESNKLGNDLTLKYPKQLGYLATLPFPYETESVTAVNKAFDQYHALGVT 124 Query: 132 LPTHALGVYLGNPCLHDIFAELNTRKAIVALHPNRPALVPEHVLERLPAPMMEFFFDTTR 191 +PT++ G Y G+P L++++ L+ R AIVA+HPN P+ +P++V LP P++ FF DTT Sbjct: 125 VPTNSRGTYFGSPTLNNVYQTLDDRHAIVAMHPNEPSALPKNVDTGLPTPLLGFFTDTTM 184 Query: 192 TVMNLIFTGTFSRFPDITFIIPHAGAFLPVLADRIAPALHMMPSIFGEHTEDA-GVDIYT 250 TVMNL+ F RFP+I IIPHAGAF+ +L DR+A E+ E DIY Sbjct: 185 TVMNLLKYRFFDRFPNIKLIIPHAGAFMAILTDRVA-----------EYVESQYDTDIYK 233 Query: 251 SLKGLYYDIAGACLPRQLDNLLQLIGTDHLLYGSDYPYTPADACKSLADGLEGTNLLTDE 310 +K +Y+D AG+ LPRQL LL L DH+LYGSD PYTP D L L TN LTD Sbjct: 234 VMKHVYFDTAGSVLPRQLPALLTLADEDHVLYGSDIPYTPLDKSAKLQQKLSNTNALTDL 293 Query: 311 ARCQIYGENALRLF 324 + +I+ +NA +L Sbjct: 294 QKQKIFHDNAAQLL 307 >C1R506.1 C1R506_9ACTO Predicted TIM-barrel fold metal-dependent hydrolase, COG2159 Length = 389 Score = 273 bits (699), Expect = 1e-71, Method: Compositional matrix adjust. Identities = 147/315 (46%), Positives = 197/315 (62%), Gaps = 11/315 (3%) Query: 14 DLHSHYLPSAYREALLKHAGPNPDGFPT-PVWHADLHLEAMEQLGIAVSLLSLSSPHIHW 72 D+H+HYL YR+AL+ PDG P+ P W AD L M+ GIAV++LS+SSP + Sbjct: 79 DVHAHYLTPTYRQALINAGITQPDGMPSIPQWSADSALATMDTTGIAVAMLSVSSPGFDF 138 Query: 73 GDREAARELARRVNEDGAELVRKYPGKFGLLASLPVPEISDSIVEIKYAMDVLHADGFTL 132 G+ +L R+VNE+GA +V+ +P +FGL+ASLP+P+I+ ++ E+ YA DVL ADG L Sbjct: 139 GEAGKVSDLVRQVNEEGAAIVKAHPTRFGLMASLPLPDINAAVAEVNYAFDVLKADGIAL 198 Query: 133 PTHALGVYLGNPCLHDIFAELNTRKAIVALHPNRPALVPEHVLERLPAPMMEFFFDTTRT 192 T+ G YLG+P + AEL+ R A+V LHP PA L P PM+EF FDTTRT Sbjct: 199 ETNYGGTYLGDPSFSPVLAELHKRNAVVHLHPTSPACWEATSLGA-PRPMIEFLFDTTRT 257 Query: 193 VMNLIFTGTFSRFPDITFIIPHAGAFLPVLADRIAPALHMMPSIFGEHTEDAGVDIYTSL 252 + LI G ++P I FI+PH GA LPVLADRI S F + T+ + VD+ +L Sbjct: 258 ITQLILGGVLLKYPGIRFIVPHTGAALPVLADRI--------SAF-DLTQPSPVDVIGAL 308 Query: 253 KGLYYDIAGACLPRQLDNLLQLIGTDHLLYGSDYPYTPADACKSLADGLEGTNLLTDEAR 312 K L+YD+AG LPR L LL L+G + LLYGSD+P+T K LA L GT +LT + + Sbjct: 309 KRLHYDVAGFALPRALPALLNLVGPETLLYGSDFPFTEDPIVKLLAAQLAGTTVLTPQQK 368 Query: 313 CQIYGENALRLFPGL 327 + NA LFP L Sbjct: 369 QAMLNGNAAGLFPRL 383 >C2FLY3.1 C2FLY3_LACPL Amidohydrolase 2 Length = 317 Score = 265 bits (677), Expect = 4e-69, Method: Compositional matrix adjust. Identities = 140/314 (44%), Positives = 201/314 (64%), Gaps = 10/314 (3%) Query: 11 NKNDLHSHYLPSAYREALLKHAGPNPDGFPTPVWHADLHLEAMEQLGIAVSLLSLSSPHI 70 +K D H+HYLP++Y EAL +H +PDG+PTP W L L M I+ S+LSLSSPH+ Sbjct: 12 SKIDFHTHYLPTSYVEALKRHVPGDPDGWPTPEWTPQLTLNFMRDNDISYSILSLSSPHV 71 Query: 71 HWGDREAARELARRVNEDGAELVRKYPGKFGLLASLPVPEISDSIVEIKYAMDVLHADGF 130 ++GD+ L N+DG L ++YP + G LASLP+P D++ ++ A+D A G Sbjct: 72 NFGDKAETIRLVEAANDDGKSLAQQYPDQLGYLASLPIPYELDAVKTVQQALDQDGALGV 131 Query: 131 TLPTHALGVYLGNPCLHDIFAELNTRKAIVALHPNRPALVPEHVLERLPAPMMEFFFDTT 190 T+PT++ G+Y G+P L ++ EL+ R+AIVALHPN PA++P++V LP P++ FF DTT Sbjct: 132 TVPTNSRGLYFGSPVLERVYQELDARQAIVALHPNEPAILPKNVDIDLPVPLLGFFMDTT 191 Query: 191 RTVMNLIFTGTFSRFPDITFIIPHAGAFLPVLADRIAPALHMMPSIFGEHTEDAGVDIYT 250 T +N++ F ++P+I IIPHAGAFL ++ DRIA + VD+Y Sbjct: 192 MTFINMLKYHFFEKYPNIKVIIPHAGAFLGIVDDRIAQYAQKV----------YQVDVYD 241 Query: 251 SLKGLYYDIAGACLPRQLDNLLQLIGTDHLLYGSDYPYTPADACKSLADGLEGTNLLTDE 310 ++ +Y+D+AGA LPRQL L+ L +HLLYGSD PYTP D + L L T+LLT+E Sbjct: 242 VMRHVYFDVAGAVLPRQLPTLMSLAQPEHLLYGSDIPYTPLDGSRQLGHALTTTDLLTNE 301 Query: 311 ARCQIYGENALRLF 324 + I+ +NA RL Sbjct: 302 QKQAIFYDNAHRLL 315 >Q88TJ2.1 Q88TJ2_LACPL Metal-dependent hydrolase (Putative) Length = 307 Score = 263 bits (673), Expect = 1e-68, Method: Compositional matrix adjust. Identities = 140/314 (44%), Positives = 200/314 (63%), Gaps = 10/314 (3%) Query: 11 NKNDLHSHYLPSAYREALLKHAGPNPDGFPTPVWHADLHLEAMEQLGIAVSLLSLSSPHI 70 +K D H+HYLP++Y EAL +H +PDG+PTP W L L M I+ S+LSLSSPH+ Sbjct: 2 SKIDFHTHYLPTSYVEALKRHVPGDPDGWPTPEWTPQLTLNFMRDNDISYSILSLSSPHV 61 Query: 71 HWGDREAARELARRVNEDGAELVRKYPGKFGLLASLPVPEISDSIVEIKYAMDVLHADGF 130 ++GD+ L N+DG L ++YP + G LASLP+P D++ ++ A+D A G Sbjct: 62 NFGDKAETIRLVEAANDDGKSLAQQYPDQLGYLASLPIPYELDAVKTVQQALDQDGALGV 121 Query: 131 TLPTHALGVYLGNPCLHDIFAELNTRKAIVALHPNRPALVPEHVLERLPAPMMEFFFDTT 190 T+PT++ G+Y G+P L ++ EL+ R+AIVALHPN PA++P++V LP P++ FF DTT Sbjct: 122 TVPTNSRGLYFGSPVLERVYQELDARQAIVALHPNEPAILPKNVDIDLPVPLLGFFMDTT 181 Query: 191 RTVMNLIFTGTFSRFPDITFIIPHAGAFLPVLADRIAPALHMMPSIFGEHTEDAGVDIYT 250 T +N++ F ++P+I IIPHAGAFL ++ DRIA + VD+Y Sbjct: 182 MTFINMLKYHFFEKYPNIKVIIPHAGAFLGIVDDRIAQYAQKV----------YQVDVYD 231 Query: 251 SLKGLYYDIAGACLPRQLDNLLQLIGTDHLLYGSDYPYTPADACKSLADGLEGTNLLTDE 310 + +Y+D+AGA LPRQL L+ L +HLLYGSD PYTP D + L L T+LLT+E Sbjct: 232 VMHHVYFDVAGAVLPRQLPTLMSLAQPEHLLYGSDIPYTPLDGSRQLGHALATTDLLTNE 291 Query: 311 ARCQIYGENALRLF 324 + I+ +NA RL Sbjct: 292 QKQAIFYDNAHRLL 305 >C2FAS4.1 C2FAS4_LACPA Amidohydrolase 2 Length = 351 Score = 240 bits (613), Expect = 1e-61, Method: Compositional matrix adjust. Identities = 124/277 (44%), Positives = 171/277 (61%), Gaps = 11/277 (3%) Query: 14 DLHSHYLPSAYREALLKHAGPNPDGFPTPVWHADLHLEAMEQLGIAVSLLSLSSPHIHWG 73 D H+H+LP Y +AL KH +PDG+PTP W L L M++ I S+LSLSSPH ++G Sbjct: 6 DFHTHFLPRLYAQALKKHIPGDPDGWPTPGWDEHLTLTFMKKNHIGYSILSLSSPHFNFG 65 Query: 74 DREAARELARRVNEDGAELVRKYPGKFGLLASLPVPEISDSIVEIKYAMDVLHADGFTLP 133 D+E +AR N+ G + + +P + G ASLP+P +S+ EI A+ A GFT+P Sbjct: 66 DKEETIAIARDANDTGERVTQDHPDQLGYFASLPLPYAQESVSEIDVAL-AHSALGFTVP 124 Query: 134 THALGVYLGNPCLHDIFAELNTRKAIVALHPNRPALVPEHVLERLPAPMMEFFFDTTRTV 193 T++ G+Y G P ++A+LN KAIV +HPNRPA P +V +P P+M FF DTT T Sbjct: 125 TNSRGLYFGTPIFDPVYAKLNQEKAIVLMHPNRPAQGPMNVNIDMPIPLMGFFIDTTMTF 184 Query: 194 MNLIFTGTFSRFPDITFIIPHAGAFLPVLADRIAPALHMMPSIFGEHTEDAGVDIYTSLK 253 MNL+ F R+PDI I+PHAGAFL +LADR A + + D++ +L+ Sbjct: 185 MNLLRYHFFDRYPDIKLIVPHAGAFLSILADRDAAFV----------KQQYNSDMFAALR 234 Query: 254 GLYYDIAGACLPRQLDNLLQLIGTDHLLYGSDYPYTP 290 +Y+D+AG PRQL LL L HL++GSD PYTP Sbjct: 235 HVYFDVAGTVFPRQLPMLLTLADEQHLVFGSDIPYTP 271 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.140 0.432 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,135,871,844 Number of extensions: 96337680 Number of successful extensions: 196553 Number of sequences better than 1.0e-05: 310 Number of HSP's gapped: 195997 Number of HSP's successfully gapped: 310 Length of query: 327 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 191 Effective length of database: 1,779,438,163 Effective search space: 339872689133 Effective search space used: 339872689133 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)