BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0568.0007.p
         (623 letters)

Database: pfamseq 
           9,421,015 sequences; 3,060,696,203 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

B3K6T0.1 B3K6T0_9BACI Penicillin-binding protein, 1A family           473   e-131
B1DG24.1 B1DG24_9BACL Penicillin-binding protein, 1A family           471   e-130
Q5WHT3.1 Q5WHT3_BACSK Penicillin-binding protein                      405   e-111
A7Z322.1 A7Z322_BACA2 PbpF                                            400   e-109
C0ZI00.1 C0ZI00_BREBN Penicillin binding protein                      397   e-108

>B3K6T0.1 B3K6T0_9BACI Penicillin-binding protein, 1A family
          Length = 893

 Score =  473 bits (1217), Expect = e-131,   Method: Compositional matrix adjust.
 Identities = 250/578 (43%), Positives = 364/578 (62%), Gaps = 9/578 (1%)

Query: 41  GSTVLDRYPEKQKLPQSSVILASDGTVLRRIPLPESGYRVTARLEEMPELLVETFLAVED 100
           G  +L++  +K     +S +   +GT++  + L +S      + E++PE L++ F+A ED
Sbjct: 64  GEKLLEQNQDKLTAYGTSKVYDRNGTLMGELSLQKSD---PVKYEDIPEKLIQAFIATED 120

Query: 101 QRFYSHQGLDYPGILRAALHNTVQLSASEGGSSITQQLARNLYLNRDKNMXXXXXXXXXX 160
           +RF  H G+D   I RAA+ + +  S  EGGS+ITQQLA+N++L RDK            
Sbjct: 121 KRFMEHNGVDMWSIGRAAVKDIMARSMVEGGSTITQQLAKNIFLTRDKTFFRKATEMSIA 180

Query: 161 XXXXXXXPKQEILQLYLNQIYMGHGQYGVKSAAEYYFGITQLKELEIWQIAALAAIPKGP 220
                   KQEI+++YLN+I  G   YG+K+A+E YFG + LKEL++W++A LAA+PKGP
Sbjct: 181 LALERQHSKQEIIEMYLNRINFGGPYYGIKAASERYFGKSDLKELDLWEMATLAAMPKGP 240

Query: 221 SVYNPEAEGERPKGRRDLVLRMMQQRGLITHAEMAAAMSKEFQPVERARAQRIGDSYMDA 280
           S YNP    +  K RR +VL +M+Q+G IT  E A A   ++      R Q+   +++D 
Sbjct: 241 SRYNPLKNPDLSKERRAVVLTLMEQQGYITAEEAAEAKKVDYAYTPPERQQKY-TAFIDY 299

Query: 281 ALKEASRLTGQPLEELRTGGYTIVTGMNPGAQQALEETFGNPEFFPPDGTTQQVEAAMAI 340
            ++EA    G   +++  GGY I T M+  AQ A+EE F NP  F       QVE +M I
Sbjct: 300 VMEEAEEKWGLTEDDVNIGGYQIYTTMDVNAQTAMEEEFNNPANFEESPDDVQVEGSMVI 359

Query: 341 INHNTGDVVALTGGRHPSIGGINRAIIDARQPGSAFKPIIDYGPALESGRFTPESILPDR 400
           IN  TG +VA+ GGR  + GG +RA    RQPGSA KPI+ Y PALESG+FT +S L ++
Sbjct: 360 INQETGGIVAMVGGREYTRGGFSRATDSRRQPGSALKPIVSYAPALESGKFTKDSRLSNK 419

Query: 401 KERYG-DYSPDNLNGIYRGQVNMALALQQSINAPAVWLLKQVGIANARSFAAKLGVTLPP 459
           K+ +G +Y P+NL+G Y   + M  A+Q+S N PAVWLL ++G+     FA  LG+ +  
Sbjct: 420 KQCFGSNYCPNNLHG-YSETIGMPEAIQRSENIPAVWLLNEIGVKTGFDFAQSLGIKMSE 478

Query: 460 EDNNLSIALGGLHTGVSPLKMAQAYSVFASGGTFHEAHTVRSIADAEGKTLYKYTSAQ-R 518
            D NLS+ALGG+ TG +  +MAQA+S FA+GG F EA  ++ I D +GK +++   ++ +
Sbjct: 479 GDANLSLALGGMDTGTNTFEMAQAFSAFANGGEFKEAFAIKQIKDNKGKVVHENKGSKGK 538

Query: 519 QVISQRTAASMTVMLRNVVNNGTGAKARMNVPVAGKTGTTQAGLPGVTGKANRDLWFVGY 578
           +V+ + TA+ MT M+R VV +GTG  AR++ PVAGKTGTTQ+G  GV+  +NRD+WFVGY
Sbjct: 539 RVMGETTASQMTDMMRRVVEDGTGKNARISRPVAGKTGTTQSGYKGVS--SNRDVWFVGY 596

Query: 579 TPDLTAAVWMGFDRTDKDNSMTAGSGKAAGLFSAVMNK 616
           TP+LTAAVWMG+D  D+ + +   S  +A L+  VM K
Sbjct: 597 TPELTAAVWMGYDNPDRQHLLKRSSSISASLWGKVMEK 634


>B1DG24.1 B1DG24_9BACL Penicillin-binding protein, 1A family
          Length = 860

 Score =  471 bits (1211), Expect = e-130,   Method: Compositional matrix adjust.
 Identities = 256/588 (43%), Positives = 364/588 (61%), Gaps = 13/588 (2%)

Query: 36  YVHQYGSTVLDRYPEKQKLPQSSVILASDGTVLRRIPLPESGYRVTARLEEMPELLVETF 95
           ++   G  +L    +K +L ++S+I  +DG  + ++    S  R  A  +EMP+LL++ F
Sbjct: 50  FITLNGERILTENADKLELGEASIIYDADGKEVTKL-YDASANREIAEYDEMPKLLLDAF 108

Query: 96  LAVEDQRFYSHQGLDYPGILRAALHNTVQLSASEGGSSITQQLARNLYLNRDKNMXXXXX 155
           +A EDQRFY H G+D+  I RA + + +  S  EG S+ITQQLA+N++L  DK       
Sbjct: 109 VATEDQRFYEHSGIDFFSIGRAVVKDVIARSKVEGASTITQQLAKNIFLTADKTFFRKAT 168

Query: 156 XXXXXXXXXXXXPKQEILQLYLNQIYMGHGQYGVKSAAEYYFGITQLKELEIWQIAALAA 215
                        KQEIL +YLN+I  G G YG+K AA+YYF  T LK+L++WQIA LA 
Sbjct: 169 EASIAVALENKYSKQEILTMYLNRITFGKGIYGIKGAADYYFD-TDLKDLKLWQIATLAG 227

Query: 216 IPKGPSVYNPEAEGERPKGRRDLVLRMMQQRGLITHAEMAAAMSKEFQPVERARAQRIGD 275
           +PK P+ YNP  + E    RR +VL++M  + LIT  EM  A +  ++P      Q+  D
Sbjct: 228 MPKAPNRYNPINDPESSMTRRAVVLKLMYDQKLITQQEMEEAKAVVYEPPANID-QKKSD 286

Query: 276 ---SYMDAALKEASR-LTGQPLEELRTGGYTIVTGMNPGAQQALEETFGNPEFFPPDGTT 331
              +Y+D  ++EA + + G   +ELR GGY I T +N  AQQA+E+ F +   F      
Sbjct: 287 PYKAYVDYVIEEAVKVMPGMTEDELRIGGYRIYTSLNTTAQQAVEKEFADDSNFEKSPDD 346

Query: 332 QQVEAAMAIINHNTGDVVALTGGRHPSIGGINRAIIDARQPGSAFKPIIDYGPALESGRF 391
           Q+ + AM I++H  G + A++GGR     G+NR  +  RQPGS+FKPI+ YGPALESG +
Sbjct: 347 QKAQGAMIIMDHRDGTIQAMSGGRDYVRKGLNRVEV-KRQPGSSFKPIVSYGPALESGDY 405

Query: 392 TPESIL-PDRKERYGDYSPDNLNGIYRGQVNMALALQQSINAPAVWLLKQVGIANARSFA 450
            P ++L  D ++ +GDY P + +G     V M  A+++S N  AVW L QVGI N  +FA
Sbjct: 406 YPWTVLNQDSQKCFGDYCPRDPHGA--APVTMQQAIKESRNLAAVWTLNQVGIGNGIAFA 463

Query: 451 AKLGVTLPPEDNNLSIALGGLHTGVSPLKMAQAYSVFASGGTFHEAHTVRSIADAEGKTL 510
            KLG+ L PED NL+IALGGL  GV+P++MA+AYSVFA+GG   + HT+  I  A  K +
Sbjct: 464 KKLGIELAPEDRNLAIALGGLTQGVTPMQMAEAYSVFANGGKSVDPHTITKIETAANKKV 523

Query: 511 YKYTS-AQRQVISQRTAASMTVMLRNVVNN-GTGAKARMNVPVAGKTGTTQAGLPGVTGK 568
           Y Y + A +Q++   TA  MT M++ V+ + GTG  AR++ PVAGKTGT+Q G+P     
Sbjct: 524 YTYHAPAAKQLMKPETAWYMTQMMQTVLESGGTGTGARIDRPVAGKTGTSQHGIPNFNSS 583

Query: 569 ANRDLWFVGYTPDLTAAVWMGFDRTDKDNSMTAGSGKAAGLFSAVMNK 616
           ANRD WFVGYTP+ TAAVWMG+D+TDK + +   S +AA +F  VM +
Sbjct: 584 ANRDAWFVGYTPEWTAAVWMGYDKTDKQHVIKKSSSQAAAMFGKVMKQ 631


>Q5WHT3.1 Q5WHT3_BACSK Penicillin-binding protein
          Length = 715

 Score =  405 bits (1040), Expect = e-111,   Method: Compositional matrix adjust.
 Identities = 242/577 (41%), Positives = 328/577 (56%), Gaps = 21/577 (3%)

Query: 50  EKQKLPQSSVILASDGTVLRRIPLPESGYRVTARLEEMPELLVETFLAVEDQRFYSHQGL 109
           EK  +  SSVI+ +DG V+  + +     R    +E++PEL+ + F+A+ED RFY HQG+
Sbjct: 37  EKLMMNNSSVIVDTDGNVITNLYMEN---REVIPIEQVPELVQQAFVAMEDSRFYEHQGI 93

Query: 110 DYPGILRAALHNTVQLSASEGGSSITQQLARNLYLNRDKNMXXXXXXXXXXXXXXXXXPK 169
           D+  I RA   + +  +  EGGS++TQQLA+NL+L+ +K                    K
Sbjct: 94  DFKAIGRALYRDILAGAKVEGGSTLTQQLAKNLFLSNEKAWLRKTNEVLIAMNLERRYSK 153

Query: 170 QEILQLYLNQIYMGHGQYGVKSAAEYYFGITQLKELEIWQIAALAAIPKGPSVYNPEAEG 229
            EIL+ YLNQ+Y GHG YG+++AA  YF      EL I++ A+L A+   PS ++P  E 
Sbjct: 154 SEILEKYLNQVYFGHGAYGIEAAAHLYFN-KPASELTIYEGASLVAVVNAPSAFSPIDEP 212

Query: 230 ERPKGRRDLVLRMMQQRGLITHAEMAAAMSKEFQPVERARAQRIGD----SYMDAALKEA 285
           ER K RRDLVL++M Q+G ++  E   A S   QP+E  R ++  D    +Y+D  + EA
Sbjct: 213 ERNKERRDLVLKVMAQQGYLSTEEAEEAQS---QPLETDRQEQTVDESLYTYIDMVMDEA 269

Query: 286 SRLTGQPLEELRTGGYTIVTGMNPGAQQALEETFGNPEFFPPDGTTQQVEAAMAIINHNT 345
           S       EEL TGGY IV  MN   Q+   E   N  FFP +    Q  +AM  ++  T
Sbjct: 270 SERFNISHEELLTGGYRIVVPMNLELQKKTYEALQNDRFFPEENKDAQ--SAMMFMDSET 327

Query: 346 GDVVALTGGRHPSIGGINRAIIDARQPGSAFKPIIDYGPALESGRFTPESILPDRKERYG 405
           G ++A+ GGR     G+NR  +  R P S FKP+  + PALESG + P S+L D   +Y 
Sbjct: 328 GGILAVQGGRDYVRRGLNRVNV-RRAPASTFKPLAVFAPALESGIYQPYSLLRDELLQYD 386

Query: 406 D-YSPDNLNGIYRGQVNMALALQQSINAPAVWLLKQVGIANARSFAAKLGVTLPPEDNNL 464
           D Y+P N+NG Y G++ M  AL  S NAPAVWLL ++GI  + S   K G  LP  D  L
Sbjct: 387 DGYTPKNVNGEYSGEMTMYEALTTSSNAPAVWLLNEIGIQESVSTLKKFGFHLP--DRQL 444

Query: 465 SIALGGLHTGVSPLKMAQAYSVFASGGTFHEAHTVRSIADAEGKTLYKYTSAQRQVISQR 524
           S+ALGGL  GV+P +M +AY  FA  G   E H +R I D+  K +      + QVIS +
Sbjct: 445 SLALGGLREGVTPYEMVRAYRAFAHEGQIIEPHVIRDIYDSNDKRIGGAKPVETQVISAQ 504

Query: 525 TAASMTVMLRNVVNNGTGAKARMNVP-VAGKTGTTQAGLPGVTGKANRDLWFVGYTPDLT 583
           TA SMT ML NV+  GTGA        VAGKTGT   G   V G AN D+WFVGYTP + 
Sbjct: 505 TAWSMTRMLENVMAAGTGASGPATTSAVAGKTGTQ--GYEAVAG-ANSDVWFVGYTPKVV 561

Query: 584 AAVWMGFDRTDKDNSMTAGSGKAAGLFSAVMNKVYAS 620
            AVWMG+D+T++      GSG    LF  ++N   AS
Sbjct: 562 GAVWMGYDQTNESQYFAGGSGYPTRLFKEMVNSFPAS 598


>A7Z322.1 A7Z322_BACA2 PbpF
          Length = 713

 Score =  400 bits (1027), Expect = e-109,   Method: Compositional matrix adjust.
 Identities = 218/539 (40%), Positives = 312/539 (57%), Gaps = 10/539 (1%)

Query: 79  RVTARLEEMPELLVETFLAVEDQRFYSHQGLDYPGILRAALHNTVQLSASEGGSSITQQL 138
           R    ++++PE + + F+AVED+RFY H G+D   + RA   + +     EGGS+ITQQL
Sbjct: 66  REPVSIQQVPEDVRDAFIAVEDKRFYEHHGIDVKSVGRAVFRDVLAGGKVEGGSTITQQL 125

Query: 139 ARNLYLNRDKNMXXXXXXXXXXXXXXXXXPKQEILQLYLNQIYMGHGQYGVKSAAEYYFG 198
           A+N++L  DK                    K ++L++YLNQ+Y GHG YG+++A+ YYF 
Sbjct: 126 AKNIFLTHDKTFLRKTKEVIIAINLERDYSKDKLLEMYLNQLYFGHGVYGIQAASHYYFN 185

Query: 199 ITQLKELEIWQIAALAAIPKGPSVYNPEAEGERPKGRRDLVLRMMQQRGLITHAEMAAAM 258
             ++K+L + + A LAAIPK PS+Y+P    ++ K RRD +L MM  +G I+  E   A 
Sbjct: 186 -KEVKDLTVAEGAVLAAIPKAPSLYSPIQHPDKNKERRDTILAMMNDQGYISAKEAVRAQ 244

Query: 259 SKEFQ-PVERARAQRIGDSYMDAALKEASRLTGQPLEELRTGGYTIVTGMNPGAQQALEE 317
            +     V++       DSY+D  +KEA        E+L  GGYTI T ++   Q+    
Sbjct: 245 GRTLGLNVKKTSETPWFDSYIDLVIKEAEDRYSMSGEQLLQGGYTIRTSLDSKLQKTAYT 304

Query: 318 TFGNPEFFPPDGTTQQVEAAMAIINHNTGDVVALTGGRHPSIGGINRAIIDARQPGSAFK 377
                 +FP  GT Q  E +   I++ TG V A  GGR     G NR +   RQPGS FK
Sbjct: 305 LMKESGYFP--GTDQAAEGSAVFIDNKTGGVRAAVGGRDYVTKGYNR-VTAKRQPGSTFK 361

Query: 378 PIIDYGPALESGRFTPESILPDRKERYGDYSPDNLNGIYRGQVNMALALQQSINAPAVWL 437
           PI  YGPA++  +F P S+L D+   Y  Y P N +G Y+G+V M  A+  SINAPAVW 
Sbjct: 362 PIAVYGPAMQEDKFKPYSLLQDKLTSYDGYEPKNYDGQYKGEVTMQDAITYSINAPAVWT 421

Query: 438 LKQVGIANARSFAAKLGVTLPPEDNNLSIALGGLHTGVSPLKMAQAYSVFASGGTFHEAH 497
           LKQ+G+   +S+  K G+ +   DN L+IALGGL  GV+PL++A AY  FA+ G + +  
Sbjct: 422 LKQIGVDTGKSYLEKNGMNI--SDNGLAIALGGLSEGVTPLQLAGAYHTFAANGMYTKPF 479

Query: 498 TVRSIADAEGKTLYKYTSAQRQVISQRTAASMTVMLRNVVNNGTGAKARMNVPVAGKTGT 557
            + +I D +G+TL    + + +V S +TA +MT ML+ VV  GT ++      +AGKTGT
Sbjct: 480 FIENITDEDGETLSGPKNGKTRVFSGQTAWNMTRMLQQVVKEGTASQGSYQGDLAGKTGT 539

Query: 558 TQAGLPGVTGKANRDLWFVGYTPDLTAAVWMGFDRTDKDNSMTAGSGKAAGLFSAVMNK 616
           T    PG+ G A +D WF GYTP LT AVWMG+D+TDK + +  GS     LF A++ K
Sbjct: 540 TT--FPGIPG-ATKDAWFAGYTPGLTGAVWMGYDKTDKTHYLKGGSQYPVRLFKAIVTK 595


>C0ZI00.1 C0ZI00_BREBN Penicillin binding protein
          Length = 838

 Score =  397 bits (1020), Expect = e-108,   Method: Compositional matrix adjust.
 Identities = 221/566 (39%), Positives = 334/566 (59%), Gaps = 12/566 (2%)

Query: 56  QSSVILASDGTVLRRIPLPESGYRVTARLEEMPELLVETFLAVEDQRFYSHQGLDYPGIL 115
           + +VI   +G  +  I L     RV   + EMP+ L++ FLAVED+RFY H+G+D   I 
Sbjct: 59  EPTVIYDKNGKEI--ISLIRQKNRVYKPINEMPKTLIDAFLAVEDKRFYEHKGVDMVRIG 116

Query: 116 RAALHNTVQLSASEGGSSITQQLARNLYLNRDKNMXXXXXXXXXXXXXXXXXPKQEILQL 175
            A +++  + S +EGGS+ITQQLARN++L  D+                    K +IL++
Sbjct: 117 GAIVNDIKKGSLAEGGSTITQQLARNVFLTLDQTFWRKTKEMSIAIGLERKYSKDQILEM 176

Query: 176 YLNQIYMGHGQYGVKSAAEYYFGITQLKELEIWQIAALAAIPKGPSVYNPEAEGERPKGR 235
           YLN++Y+G G++GV+ AA+YYFG + +  + + + A LAAIPK PS Y+P    E+ K R
Sbjct: 177 YLNRVYLGEGEFGVEDAAQYYFGKS-VSNVTVAEAAMLAAIPKAPSTYSPFNNPEKAKLR 235

Query: 236 RDLVLRMMQQRGLITHAEMAAAMSKEF--QPVERA--RAQRIGDSYMDAALKEASRLTGQ 291
           RD V+R+M ++G+IT+ +  AA ++    +P E A    ++   ++ D  LKE+    G 
Sbjct: 236 RDTVIRLMSEQGIITNEQKDAAQAEALPTKPHEVAGGSLKKGYRAFFDNMLKESEAEFGV 295

Query: 292 PLEELRTGGYTIVTGMNPGAQQALEETFGNPEFFPPDGTTQQVEAAMAIINHNTGDVVAL 351
             EEL  GG+ I T  +P  Q A+ E + N + FP DG  + VE++M +I+  TG +  +
Sbjct: 296 TEEELYRGGWDIYTTFDPKVQDAMVEEYANAKNFPKDGAKRGVESSMIVIDAKTGGIAGM 355

Query: 352 TGGRHPSIGGINRAIIDARQPGSAFKPIIDYGPALES--GRFTPESILPDRKERYGDYSP 409
            GGR+ +  G N A    RQPGS+FKPI  + PA+     ++   S L ++++ +  Y P
Sbjct: 356 IGGRNYAPKGFNYATDMKRQPGSSFKPIAVFAPAVNKDINKWNANSQLSNKRQSFNGYEP 415

Query: 410 DNLNGIYRGQVNMALALQQSINAPAVWLLKQVGIANARSFAAKLGVTLPPEDNNLSIALG 469
            N N  Y   V+M+ A+  S N P VWLL ++G++ + ++  K+G+ L P D NL+IALG
Sbjct: 416 RNYNNRYTETVSMSRAMIDSANIPPVWLLNEIGVSESLAYLKKVGIELTPNDRNLAIALG 475

Query: 470 GLHTGVSPLKMAQAYSVFASGGTFHEAHTVRSIADAEGKTLYKYTSAQRQVISQRTAASM 529
           GL+ G+SP +MAQAY+ F + G   EAH++  + + +   + +Y   Q + +    A  +
Sbjct: 476 GLNQGISPKQMAQAYTPFVNAGVVSEAHSISKVENKDEGVVREYQPKQSEALKPEAAWEI 535

Query: 530 TVMLRNVVNNGTGAKARMN-VPVAGKTGTTQAGLPGVTGKANRDLWFVGYTPDLTAAVWM 588
             ML   V  GTG  AR++   VAGKTGTTQ+ + G + KAN+D WFVGYTP+   AVWM
Sbjct: 536 HTMLEKAVQEGTGKAARISGRHVAGKTGTTQS-IAGDS-KANKDAWFVGYTPEYVGAVWM 593

Query: 589 GFDRTDKDNSMTAGSGKAAGLFSAVM 614
           GFD  DK + M  GS   A +FS VM
Sbjct: 594 GFDPEDKQHLMHQGSSMTAQMFSKVM 619


  Database: pfamseq
    Posted date:  Aug 5, 2010  3:22 PM
  Number of letters in database: 3,060,696,203
  Number of sequences in database:  9,421,015
  
Lambda     K      H
   0.316    0.133    0.381 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 9421015
Number of Hits to DB: 3,604,649,394
Number of extensions: 152093235
Number of successful extensions: 381071
Number of sequences better than 1.0e-05: 5310
Number of HSP's gapped: 357092
Number of HSP's successfully gapped: 6649
Length of query: 623
Length of database: 3,060,696,203
Length adjustment: 142
Effective length of query: 481
Effective length of database: 1,722,912,073
Effective search space: 828720707113
Effective search space used: 828720707113
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 134 (56.2 bits)