BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0567.0031.p (304 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q97TR8.1 Q97TR8_CLOAB Permease MDR-related 441 e-122 A6LXC0.1 A6LXC0_CLOB8 Major facilitator superfamily MFS_1 438 e-121 A6LYL4.1 A6LYL4_CLOB8 Major facilitator superfamily MFS_1 437 e-121 B9E432.1 B9E432_CLOK1 Putative uncharacterized protein 424 e-117 A5N061.1 A5N061_CLOK5 Predicted permease 423 e-117 >Q97TR8.1 Q97TR8_CLOAB Permease MDR-related Length = 399 Score = 441 bits (1133), Expect = e-122, Method: Compositional matrix adjust. Identities = 211/302 (69%), Positives = 256/302 (84%) Query: 1 MGFVHNVYILIGLRLLQGTITGYGTACTTLIATQTDKEHAGYALGTLSTASIAGSLLGPS 60 MGFV NVY+LI LRLLQG ITGY TACTTLIATQTDKEHAG+ALGTLSTA IAGSLLGP+ Sbjct: 93 MGFVSNVYVLIVLRLLQGVITGYSTACTTLIATQTDKEHAGWALGTLSTAGIAGSLLGPT 152 Query: 61 LGGFIAENMGLEDXXXXXXXXXXXXXXXXXXXVQESFVREDKQALRFKEVWSSVPEKNLT 120 +GGF+ E G ++ V+E FVRE+K+ K+VW+SVPEK+LT Sbjct: 153 IGGFVEEAFGTQNVFFITGALLIIVFIATMLFVKEDFVRENKKVDSMKKVWNSVPEKSLT 212 Query: 121 ITLFVSFFVLTVALYTVEPVITVYVNHLSRSGSHIALIAGLTFSASGLANIIAAPRLGRL 180 IT+ V+FF+LT+ALY++EP++T YV+ LSR+ SH+A IAGL FSASGLANI+AAPRLG++ Sbjct: 213 ITILVTFFILTLALYSIEPIMTEYVSKLSRNTSHVAFIAGLVFSASGLANIVAAPRLGKI 272 Query: 181 SDRIGAHRVMLAALITAGLIFIPQAFVQNPWQLMGLRFVLGLAAGGLIPSVNILVKKITP 240 SD+IG H++ML ALI AG++FIPQAFV+NPWQLM RF+LGLA+GGL PSVNILVKKITP Sbjct: 273 SDKIGPHKIMLGALIVAGIVFIPQAFVKNPWQLMFFRFLLGLASGGLTPSVNILVKKITP 332 Query: 241 SSITGRVFGLNMSAGYLGIFGGSVLGGQIAAWFGIRYVFFITAALLLINAVWVYFKVYKK 300 ++ITGR+FGL+MSAGYLG+FGGSVLGGQ++A+ GIRYVFFIT++LLLINAVWVYFKVYKK Sbjct: 333 TAITGRIFGLSMSAGYLGVFGGSVLGGQVSAYLGIRYVFFITSSLLLINAVWVYFKVYKK 392 Query: 301 LN 302 LN Sbjct: 393 LN 394 Score = 70.1 bits (170), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 52/174 (29%), Positives = 86/174 (49%), Gaps = 3/174 (1%) Query: 116 EKNLTITLFVSFFVLTVALYTVEPVITVYVNHLS-RSGSHIALIAGLTFSASGLANIIAA 174 +KNL + F F+ ++ + + PV+ +Y+ HL S S + +G+ F + + + I + Sbjct: 5 KKNLIVCWF-GMFITSIGMSQIAPVLPLYIRHLGVNSSSLVEEFSGIAFGITFIVSAIFS 63 Query: 175 PRLGRLSDRIGAHRVMLAALITAGLIFIPQAFVQNPWQLMGLRFVLGLAAGGLIPSVNIL 234 P G+ +D+ G ++L A G+I FV N + L+ LR + G+ G + L Sbjct: 64 PIWGQAADKFGRKPMLLRASFGMGIIIFSMGFVSNVYVLIVLRLLQGVIT-GYSTACTTL 122 Query: 235 VKKITPSSITGRVFGLNMSAGYLGIFGGSVLGGQIAAWFGIRYVFFITAALLLI 288 + T G G +AG G G +GG + FG + VFFIT ALL+I Sbjct: 123 IATQTDKEHAGWALGTLSTAGIAGSLLGPTIGGFVEEAFGTQNVFFITGALLII 176 >A6LXC0.1 A6LXC0_CLOB8 Major facilitator superfamily MFS_1 Length = 397 Score = 438 bits (1126), Expect = e-121, Method: Compositional matrix adjust. Identities = 211/302 (69%), Positives = 252/302 (83%) Query: 1 MGFVHNVYILIGLRLLQGTITGYGTACTTLIATQTDKEHAGYALGTLSTASIAGSLLGPS 60 MGF NVY+LIGLRLLQG ITGYGTACT LIATQTDKE+AGYALGTLSTA+IAGSL+GP+ Sbjct: 93 MGFAPNVYVLIGLRLLQGVITGYGTACTALIATQTDKENAGYALGTLSTANIAGSLIGPT 152 Query: 61 LGGFIAENMGLEDXXXXXXXXXXXXXXXXXXXVQESFVREDKQALRFKEVWSSVPEKNLT 120 +GGFI E GL+ V+ESF REDK L KEVW ++PEKNLT Sbjct: 153 IGGFIDEIFGLQSVFFITAALLLISFITTVLFVKESFTREDKDVLSIKEVWKAIPEKNLT 212 Query: 121 ITLFVSFFVLTVALYTVEPVITVYVNHLSRSGSHIALIAGLTFSASGLANIIAAPRLGRL 180 IT+FV+FF+L VALY+VEP++TVYV LS + SHI L+AGLTFSASGLANIIAAPRLG+L Sbjct: 213 ITMFVTFFILAVALYSVEPIVTVYVKQLSSNSSHIVLLAGLTFSASGLANIIAAPRLGKL 272 Query: 181 SDRIGAHRVMLAALITAGLIFIPQAFVQNPWQLMGLRFVLGLAAGGLIPSVNILVKKITP 240 SD++GAH+++LA +I AG+IFIPQAFV++ WQLMGLRF+LG+A+ GL PSVNI++KKITP Sbjct: 273 SDKVGAHKIILACIIGAGIIFIPQAFVKDTWQLMGLRFLLGIASAGLNPSVNIVLKKITP 332 Query: 241 SSITGRVFGLNMSAGYLGIFGGSVLGGQIAAWFGIRYVFFITAALLLINAVWVYFKVYKK 300 +S+TGRVFG NMSAGYLG FGG+VLGGQ+A G++YVFFIT+ALL INAVWVYFKVYKK Sbjct: 333 ASLTGRVFGFNMSAGYLGAFGGAVLGGQVAGNLGMKYVFFITSALLFINAVWVYFKVYKK 392 Query: 301 LN 302 L+ Sbjct: 393 LS 394 Score = 68.9 bits (167), Expect = 6e-10, Method: Compositional matrix adjust. Identities = 55/182 (30%), Positives = 88/182 (48%), Gaps = 7/182 (3%) Query: 109 EVWSSVPEKNLTITLFVSFFVLTVALYTVEPVITVYVNHLS-RSGSHIALIAGLTFSASG 167 E+W KNL + F FV + + + PV+ +Y+ HL + + I I+G+ F + Sbjct: 2 EIW----RKNLIVCWF-GIFVAAIGMSQIAPVLPIYIQHLGVQDTASITKISGIAFGITY 56 Query: 168 LANIIAAPRLGRLSDRIGAHRVMLAALITAGLIFIPQAFVQNPWQLMGLRFVLGLAAGGL 227 + + I +P G +D+ G ++L A + + F N + L+GLR + G+ G Sbjct: 57 VISAIFSPIWGSAADKYGRKPMILRASLGMAITIGCMGFAPNVYVLIGLRLLQGVIT-GY 115 Query: 228 IPSVNILVKKITPSSITGRVFGLNMSAGYLGIFGGSVLGGQIAAWFGIRYVFFITAALLL 287 + L+ T G G +A G G +GG I FG++ VFFITAALLL Sbjct: 116 GTACTALIATQTDKENAGYALGTLSTANIAGSLIGPTIGGFIDEIFGLQSVFFITAALLL 175 Query: 288 IN 289 I+ Sbjct: 176 IS 177 >A6LYL4.1 A6LYL4_CLOB8 Major facilitator superfamily MFS_1 Length = 418 Score = 437 bits (1123), Expect = e-121, Method: Compositional matrix adjust. Identities = 212/302 (70%), Positives = 250/302 (82%) Query: 1 MGFVHNVYILIGLRLLQGTITGYGTACTTLIATQTDKEHAGYALGTLSTASIAGSLLGPS 60 MGF NVY+LI LRLLQG ITGY TACTTLIATQTDKEHAGYALGT+STA+IAGSLLGP+ Sbjct: 93 MGFAPNVYVLIALRLLQGAITGYSTACTTLIATQTDKEHAGYALGTISTANIAGSLLGPT 152 Query: 61 LGGFIAENMGLEDXXXXXXXXXXXXXXXXXXXVQESFVREDKQALRFKEVWSSVPEKNLT 120 +GGF+ E GL++ V+ESF RED + L FKEVW++VPEK LT Sbjct: 153 IGGFVGETFGLQNVFFITGGLMLIAFITTVLFVKESFSREDNKVLSFKEVWNTVPEKGLT 212 Query: 121 ITLFVSFFVLTVALYTVEPVITVYVNHLSRSGSHIALIAGLTFSASGLANIIAAPRLGRL 180 ITLF++FF+L+VA+Y+VEP++TVYV LS+ SHIALIAG+TFSASG ANIIAAPRLGR+ Sbjct: 213 ITLFITFFILSVAMYSVEPIVTVYVTQLSKEASHIALIAGITFSASGFANIIAAPRLGRI 272 Query: 181 SDRIGAHRVMLAALITAGLIFIPQAFVQNPWQLMGLRFVLGLAAGGLIPSVNILVKKITP 240 SD++GAH+VML LI AG+IFIPQAFV N W+LMGLRF+LGL GGL PSVNILVKKITP Sbjct: 273 SDKVGAHKVMLICLIIAGIIFIPQAFVTNTWELMGLRFLLGLTVGGLNPSVNILVKKITP 332 Query: 241 SSITGRVFGLNMSAGYLGIFGGSVLGGQIAAWFGIRYVFFITAALLLINAVWVYFKVYKK 300 S++TGRVFG +M+AGYLG+F GSVLGGQIA + GI+ VFF+T+ALLLINAV VYFKVY K Sbjct: 333 SALTGRVFGFSMAAGYLGVFAGSVLGGQIAGYLGIKCVFFVTSALLLINAVLVYFKVYNK 392 Query: 301 LN 302 LN Sbjct: 393 LN 394 Score = 65.9 bits (159), Expect = 4e-09, Method: Compositional matrix adjust. Identities = 49/181 (27%), Positives = 87/181 (48%), Gaps = 7/181 (3%) Query: 109 EVWSSVPEKNLTITLFVSFFVLTVALYTVEPVITVYVNHLS-RSGSHIALIAGLTFSASG 167 E+W ++NL + F FV + + + PV+ +Y+ HL + + I ++G+ F ++ Sbjct: 2 EMW----KRNLKVCWF-GMFVTGIGMSQIAPVLPLYIQHLGIQDAASIERLSGIAFGSTF 56 Query: 168 LANIIAAPRLGRLSDRIGAHRVMLAALITAGLIFIPQAFVQNPWQLMGLRFVLGLAAGGL 227 + + I +P G +D+ G ++L A + ++ F N + L+ LR + G A G Sbjct: 57 IISAIFSPIWGHAADKFGRKPMLLRASLGMAIVISCMGFAPNVYVLIALRLLQG-AITGY 115 Query: 228 IPSVNILVKKITPSSITGRVFGLNMSAGYLGIFGGSVLGGQIAAWFGIRYVFFITAALLL 287 + L+ T G G +A G G +GG + FG++ VFFIT L+L Sbjct: 116 STACTTLIATQTDKEHAGYALGTISTANIAGSLLGPTIGGFVGETFGLQNVFFITGGLML 175 Query: 288 I 288 I Sbjct: 176 I 176 >B9E432.1 B9E432_CLOK1 Putative uncharacterized protein Length = 407 Score = 424 bits (1089), Expect = e-117, Method: Compositional matrix adjust. Identities = 202/302 (66%), Positives = 248/302 (82%) Query: 1 MGFVHNVYILIGLRLLQGTITGYGTACTTLIATQTDKEHAGYALGTLSTASIAGSLLGPS 60 MGF NVY LI LR+LQG ITGY TAC TLIATQTD+EHAG+ALGTLST+SIAGSLLGP Sbjct: 104 MGFAQNVYELIILRVLQGIITGYSTACITLIATQTDREHAGWALGTLSTSSIAGSLLGPM 163 Query: 61 LGGFIAENMGLEDXXXXXXXXXXXXXXXXXXXVQESFVREDKQALRFKEVWSSVPEKNLT 120 +GG+I EN L++ V+ESF REDK+ KE+WS +P K+LT Sbjct: 164 IGGYIEENFSLQNVFFITGVLMLITFTTTALFVRESFTREDKKVSSIKEIWSIIPNKSLT 223 Query: 121 ITLFVSFFVLTVALYTVEPVITVYVNHLSRSGSHIALIAGLTFSASGLANIIAAPRLGRL 180 I +FV+ F+LT+ALY++EP+ITVY+ LSR+ +H+AL+AG+ FS SGLANIIAAPRLG+L Sbjct: 224 IIMFVTSFILTLALYSIEPIITVYIAQLSRNSNHVALLAGMAFSVSGLANIIAAPRLGKL 283 Query: 181 SDRIGAHRVMLAALITAGLIFIPQAFVQNPWQLMGLRFVLGLAAGGLIPSVNILVKKITP 240 SD+IGA +V+L AL+ AG+IFIPQAFV+NPWQLMGLRF+LGLA GGL PSVN+LVK+ITP Sbjct: 284 SDKIGAQKVILVALVAAGIIFIPQAFVKNPWQLMGLRFLLGLAMGGLNPSVNVLVKRITP 343 Query: 241 SSITGRVFGLNMSAGYLGIFGGSVLGGQIAAWFGIRYVFFITAALLLINAVWVYFKVYKK 300 S+TGRVFG N+SA YLGIFGGS+LGGQ+AA+ GIRY+FFIT+ LLL+NAVWVYFKVYK+ Sbjct: 344 DSLTGRVFGFNISAQYLGIFGGSILGGQVAAYLGIRYIFFITSTLLLLNAVWVYFKVYKR 403 Query: 301 LN 302 L+ Sbjct: 404 LS 405 Score = 65.5 bits (158), Expect = 6e-09, Method: Compositional matrix adjust. Identities = 50/195 (25%), Positives = 95/195 (48%), Gaps = 10/195 (5%) Query: 96 SFVREDKQALRFKEVWSSVPEKNLTITLFVSFFVLTVALYTVEPVITVYVNHLSRSGSH- 154 S+ +E + ++ VW ++NL + F FV +V + + PV+ +Y+ HL S + Sbjct: 3 SYSKEKRYIMK---VW----KRNLIVCWF-GMFVTSVGMSQIAPVLPLYIKHLGISNTAL 54 Query: 155 IALIAGLTFSASGLANIIAAPRLGRLSDRIGAHRVMLAALITAGLIFIPQAFVQNPWQLM 214 I +G+ F + + + + +P G +D+ G ++L A + ++ F QN ++L+ Sbjct: 55 IEQFSGIAFGITFIISAVFSPVWGHAADKFGRKPMLLRASLGMAIVIFSMGFAQNVYELI 114 Query: 215 GLRFVLGLAAGGLIPSVNILVKKITPSSITGRVFGLNMSAGYLGIFGGSVLGGQIAAWFG 274 LR + G+ G + ++ + T G G ++ G G ++GG I F Sbjct: 115 ILRVLQGIITGYSTACITLIATQ-TDREHAGWALGTLSTSSIAGSLLGPMIGGYIEENFS 173 Query: 275 IRYVFFITAALLLIN 289 ++ VFFIT L+LI Sbjct: 174 LQNVFFITGVLMLIT 188 >A5N061.1 A5N061_CLOK5 Predicted permease Length = 396 Score = 423 bits (1088), Expect = e-117, Method: Compositional matrix adjust. Identities = 202/302 (66%), Positives = 248/302 (82%) Query: 1 MGFVHNVYILIGLRLLQGTITGYGTACTTLIATQTDKEHAGYALGTLSTASIAGSLLGPS 60 MGF NVY LI LR+LQG ITGY TAC TLIATQTD+EHAG+ALGTLST+SIAGSLLGP Sbjct: 93 MGFAQNVYELIILRVLQGIITGYSTACITLIATQTDREHAGWALGTLSTSSIAGSLLGPM 152 Query: 61 LGGFIAENMGLEDXXXXXXXXXXXXXXXXXXXVQESFVREDKQALRFKEVWSSVPEKNLT 120 +GG+I EN L++ V+ESF REDK+ KE+WS +P K+LT Sbjct: 153 IGGYIEENFSLQNVFFITGVLMLITFTTTALFVRESFTREDKKVSSIKEIWSIIPNKSLT 212 Query: 121 ITLFVSFFVLTVALYTVEPVITVYVNHLSRSGSHIALIAGLTFSASGLANIIAAPRLGRL 180 I +FV+ F+LT+ALY++EP+ITVY+ LSR+ +H+AL+AG+ FS SGLANIIAAPRLG+L Sbjct: 213 IIMFVTSFILTLALYSIEPIITVYIAQLSRNSNHVALLAGMAFSVSGLANIIAAPRLGKL 272 Query: 181 SDRIGAHRVMLAALITAGLIFIPQAFVQNPWQLMGLRFVLGLAAGGLIPSVNILVKKITP 240 SD+IGA +V+L AL+ AG+IFIPQAFV+NPWQLMGLRF+LGLA GGL PSVN+LVK+ITP Sbjct: 273 SDKIGAQKVILVALVAAGIIFIPQAFVKNPWQLMGLRFLLGLAMGGLNPSVNVLVKRITP 332 Query: 241 SSITGRVFGLNMSAGYLGIFGGSVLGGQIAAWFGIRYVFFITAALLLINAVWVYFKVYKK 300 S+TGRVFG N+SA YLGIFGGS+LGGQ+AA+ GIRY+FFIT+ LLL+NAVWVYFKVYK+ Sbjct: 333 DSLTGRVFGFNISAQYLGIFGGSILGGQVAAYLGIRYIFFITSTLLLLNAVWVYFKVYKR 392 Query: 301 LN 302 L+ Sbjct: 393 LS 394 Score = 63.9 bits (154), Expect = 2e-08, Method: Compositional matrix adjust. Identities = 48/182 (26%), Positives = 89/182 (48%), Gaps = 7/182 (3%) Query: 109 EVWSSVPEKNLTITLFVSFFVLTVALYTVEPVITVYVNHLSRSGSH-IALIAGLTFSASG 167 +VW ++NL + F FV +V + + PV+ +Y+ HL S + I +G+ F + Sbjct: 2 KVW----KRNLIVCWF-GMFVTSVGMSQIAPVLPLYIKHLGISNTALIEQFSGIAFGITF 56 Query: 168 LANIIAAPRLGRLSDRIGAHRVMLAALITAGLIFIPQAFVQNPWQLMGLRFVLGLAAGGL 227 + + + +P G +D+ G ++L A + ++ F QN ++L+ LR + G+ G Sbjct: 57 IISAVFSPVWGHAADKFGRKPMLLRASLGMAIVIFSMGFAQNVYELIILRVLQGIITGYS 116 Query: 228 IPSVNILVKKITPSSITGRVFGLNMSAGYLGIFGGSVLGGQIAAWFGIRYVFFITAALLL 287 + ++ + T G G ++ G G ++GG I F ++ VFFIT L+L Sbjct: 117 TACITLIATQ-TDREHAGWALGTLSTSSIAGSLLGPMIGGYIEENFSLQNVFFITGVLML 175 Query: 288 IN 289 I Sbjct: 176 IT 177 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.325 0.141 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,609,990,039 Number of extensions: 62135811 Number of successful extensions: 342260 Number of sequences better than 1.0e-05: 1664 Number of HSP's gapped: 345735 Number of HSP's successfully gapped: 2287 Length of query: 304 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 169 Effective length of database: 1,788,859,178 Effective search space: 302317201082 Effective search space used: 302317201082 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 131 (55.1 bits)