BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0567.0012.p (335 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K471.1 B3K471_9BACI Alcohol dehydrogenase zinc-binding domain ... 334 6e-90 B7R6C0.1 B7R6C0_9THEO Oxidoreductase, zinc-binding dehydrogenase... 270 2e-70 Q8PSM7.1 Q8PSM7_METMA Oxidoreductase EC=1.1.1.- 205 6e-51 B5YJC6.1 B5YJC6_THEYD Oxidoreductase 200 1e-49 Q8PXN6.1 Q8PXN6_METMA Oxidoreductase EC=1.1.1.- 189 2e-46 >B3K471.1 B3K471_9BACI Alcohol dehydrogenase zinc-binding domain protein Length = 341 Score = 334 bits (857), Expect = 6e-90, Method: Compositional matrix adjust. Identities = 182/335 (54%), Positives = 225/335 (67%), Gaps = 5/335 (1%) Query: 1 MRIVAAKEGKVAILQAAIP-ELNKRHVQVRTEYSGISPGTELSSIKRSGLSPVSLGYSAV 59 M+ VA G V + P EL HV++RTEYS +SPGTE+ I+++ V +GYSAV Sbjct: 1 MKAVATIHGNVIVDHIERPAELPAGHVRIRTEYSAVSPGTEMIFIRKASDQKVIMGYSAV 60 Query: 60 GIVEQTGSEVRDIHPGDRVACYGVPYVRHAEVISVPTNLVTKVPHHVNPEEAAFTGLGAI 119 GIVE G V+ + G RVACYG PYVRHA+ ++VP+NL VP V+P+EAAF GLGAI Sbjct: 61 GIVEDLGEGVKGLAVGQRVACYGAPYVRHAQWLTVPSNLTAIVPDGVDPQEAAFAGLGAI 120 Query: 120 AIHALRTADVRFXXXXXXXXXXXXXXXXAQIASAAACHTAAYDLSEDRVQLLQGQMGITS 179 AIHALR AD+RF AQIA AAAC A +DL+ +RVQ ++ I Sbjct: 121 AIHALRVADLRFGESAVVVGLGILGQIIAQIAHAAACPVAGFDLNPERVQGMRQH--IAY 178 Query: 180 GFSSEEEVEHFVMNETGGAGFDSILLCAGGPGEVLINKSLEWLRDLGKIVIVGDLSMEFS 239 + + E++E V TGG G DS++LCA GPGE LIN S+ W+RD GKIVIVGDLS +S Sbjct: 179 AYDNREQLEEQVGRMTGGHGADSVILCASGPGEELINASMGWIRDKGKIVIVGDLSTTYS 238 Query: 240 RDLMFRKEAQILISRAGGPGRYDMRYEEDNQDYPIGFVRWTEGRNMDEYVRLLAEKRITV 299 R LMF KEAQ+LISRAGGPGRYD YE +N DYPIGFVRWTEGRN+ EY+RLLAE RI++ Sbjct: 239 RGLMFGKEAQVLISRAGGPGRYDASYELNNTDYPIGFVRWTEGRNIAEYIRLLAEGRISI 298 Query: 300 SPAITHRFALEEAPAAYGIYQSGPAQGALATLIKY 334 P I+H ++EA AY Y GP L T+ KY Sbjct: 299 KPLISHVVPVDEAGKAYERY--GPGSTTLGTIFKY 331 >B7R6C0.1 B7R6C0_9THEO Oxidoreductase, zinc-binding dehydrogenase family Length = 344 Score = 270 bits (689), Expect = 2e-70, Method: Compositional matrix adjust. Identities = 145/339 (42%), Positives = 200/339 (58%), Gaps = 8/339 (2%) Query: 1 MRIVAAKEGKVAILQAAIPELNKRHVQVRTEYSGISPGTELSSIKRSGLSPVSLGYSAVG 60 MR + EGKV + + P+L +R + VRT YS IS GTEL+ I +P LGYSAVG Sbjct: 6 MRAILTCEGKVYLDEVEEPQLGERTLLVRTLYSAISTGTELNLISSKTKNPFRLGYSAVG 65 Query: 61 IVEQTGSEVRDIHPGDRVACYGVPYVRHAEVISVPTNLVTKVPHHVNPEEAAFTGLGAIA 120 +VE+ G+ V+ G+ VACYG PYV H E +SVP L KVP V+ +EAAF GLGAIA Sbjct: 66 VVEEIGNGVKGFEIGNLVACYGAPYVFHGEKLSVPITLCAKVPQGVSLKEAAFVGLGAIA 125 Query: 121 IHALRTADVRFXXXXXXXXXXXXXXXXAQIASAAACHTAAYDLSEDRVQL-----LQGQM 175 IH +R A+++F QI++ A S + ++ Q Sbjct: 126 IHGIRKANLQFGESVVILGLGLLGQLSYQISAHANYRVIGVSYSNEHAEIAKKFNYQNGH 185 Query: 176 GITSGFSSEEEVEHFVMNETGGAGFDSILLCAGGPGEVLINKSLEWLRDLGKIVIVGDLS 235 I +E+ + ++ T G G D++++ A P LI+ +L+W+R GKIVIVG++ Sbjct: 186 VINLQEENEDSLLKYIDEITEGNGVDAVVITAHSPDTKLIDNALKWVRVGGKIVIVGNVR 245 Query: 236 MEFSRDLMFRKEAQILISRAGGPGRYDMRYEEDNQDYPIGFVRWTEGRNMDEYVRLLAEK 295 MEF R+ F+KEA++LISRA GPGRYD+ YE++ DYP+ VRWTEGRNM EY+RLL EK Sbjct: 246 MEFDREAFFQKEAEVLISRAAGPGRYDLNYEKNAVDYPVSLVRWTEGRNMKEYIRLLKEK 305 Query: 296 RITVSPAITHRFALEEAPAAYGIYQSGPAQGALATLIKY 334 +I +SP I + E+AP Y + P L T+ KY Sbjct: 306 KIVISPFIKE-YPFEKAPEVYSSLKKEP--NILGTIFKY 341 >Q8PSM7.1 Q8PSM7_METMA Oxidoreductase EC=1.1.1.- Length = 720 Score = 205 bits (521), Expect = 6e-51, Method: Compositional matrix adjust. Identities = 126/376 (33%), Positives = 190/376 (50%), Gaps = 53/376 (14%) Query: 3 IVAAKEGKVAILQAAIPELNKRHVQVRTEYSGISPGTE---------------------- 40 ++ K+G + + + IP + V VR YS IS GTE Sbjct: 5 VLNLKDGDLTVEEVPIPTIKGSGVLVRNHYSVISAGTESGVVDLADKSLIGKARARPDLA 64 Query: 41 ---LSSIKRSGLSPVS--------------LGYSAVGIVEQTGSEVRDIHPGDRVACYGV 83 ++ K+ G P+S LGYS+ G V +++DI PGDRVAC G Sbjct: 65 MKVINKAKQDG--PISAFQQAMGRLEKREPLGYSSAGTVISVSEDIKDIKPGDRVACAGA 122 Query: 84 PYVRHAEVISVPTNLVTKVPHHVNPEEAAFTGLGAIAIHALRTADVRFXXXXXXXXXXXX 143 Y HA+V+ VP NL KVP +V+ + A FT +G+IA+ +R ADV+ Sbjct: 123 GYANHADVVFVPRNLCAKVPENVDFKNACFTTVGSIAMQGVRNADVKIGENIVVIGLGLI 182 Query: 144 XXXXAQIASAAACHTAAYDLSEDRVQLLQGQMGITSGFSS-----EEEVEHFVMNETGGA 198 QI A+ C DL E +V L + ++G +S EE ++ F + G Sbjct: 183 GLITLQILKASGCRVFGVDLDESKVNLAR-ELGADLSYSRNSPNLEERIKQF----SRGI 237 Query: 199 GFDSILLCAGGPGEVLINKSLEWLRDLGKIVIVGDLSMEFSRDLMFRKEAQILISRAGGP 258 G DS ++ A ++ + + R+ GK+VIVG + M+ R+ + KE ++ ISR+ GP Sbjct: 238 GADSTIITAATRSNDPVDFAGKITRERGKVVIVGLVGMDIPREEYYNKELEMRISRSYGP 297 Query: 259 GRYDMRYEEDNQDYPIGFVRWTEGRNMDEYVRLLAEKRITVSPAITHRFALEEAPAAYGI 318 GRYD YEE QDYP +VRWTE RNM ++ L++ K+I++ P ITH + +E+AP AY I Sbjct: 298 GRYDRNYEEYGQDYPAPYVRWTENRNMQAFLDLISMKKISMEPIITHEYEIEQAPEAYNI 357 Query: 319 YQSGPAQGALATLIKY 334 + + L ++KY Sbjct: 358 IKE--RKPYLGLVLKY 371 >B5YJC6.1 B5YJC6_THEYD Oxidoreductase Length = 703 Score = 200 bits (509), Expect = 1e-49, Method: Compositional matrix adjust. Identities = 102/285 (35%), Positives = 162/285 (56%), Gaps = 1/285 (0%) Query: 50 SPVSLGYSAVGIVEQTGSEVRDIHPGDRVACYGVPYVRHAEVISVPTNLVTKVPHHVNPE 109 +PV LGYS+ GIV + G + PGDRVAC G Y HAE ++VP NL ++P +V+ E Sbjct: 70 NPVPLGYSSAGIVVEVGRNIHKFAPGDRVACIGAGYASHAEYVAVPENLCCRIPENVSFE 129 Query: 110 EAAFTGLGAIAIHALRTADVRFXXXXXXXXXXXXXXXXAQIASAAACHTAAYDLSEDRVQ 169 EA+F LG IA+H +R ++ F QI A +D+ E +V+ Sbjct: 130 EASFGMLGIIAMHGIRCGNLTFGEKVAVIGLGLLGLLTIQILKAYGFMVMGFDIDESKVE 189 Query: 170 LLQGQMGITSGFSSEEEVEHFVMNETGGAGFDSILLCAGGPGEVLINKSLEWLRDLGKIV 229 + + ++G+ + S E E+ V T G G D++++ A + I+ ++E R G+IV Sbjct: 190 MAR-KLGVDAVSSDAEVFENLVDKYTNGFGADAVIITAATKSDAPIHLAVEIARFRGRIV 248 Query: 230 IVGDLSMEFSRDLMFRKEAQILISRAGGPGRYDMRYEEDNQDYPIGFVRWTEGRNMDEYV 289 +VG + R+ ++ KE ++++S+A GPG D YE DYP+G+VRWTE RN++E++ Sbjct: 249 VVGVADIHPDRNELWHKELELIVSKAAGPGSLDPIYENKGIDYPLGYVRWTENRNLEEFL 308 Query: 290 RLLAEKRITVSPAITHRFALEEAPAAYGIYQSGPAQGALATLIKY 334 RL++EK++ + ITHRF +EEA Y S + ++KY Sbjct: 309 RLVSEKKVNLEQLITHRFQIEEAVQVYENMMSNRGGPYVGVVLKY 353 >Q8PXN6.1 Q8PXN6_METMA Oxidoreductase EC=1.1.1.- Length = 724 Score = 189 bits (481), Expect = 2e-46, Method: Compositional matrix adjust. Identities = 121/358 (33%), Positives = 178/358 (49%), Gaps = 43/358 (12%) Query: 3 IVAAKEGKVAILQAAIPELNKRHVQVRTEYSGISPGTE---------------------- 40 ++ K+G + + IP L V VR YS IS GTE Sbjct: 11 VLNLKDGDLTVEDVPIPTLKGSGVLVRNYYSVISAGTESGLVDLADKSLIGKARARPDLA 70 Query: 41 ---LSSIKRSGLSPVS--------------LGYSAVGIVEQTGSEVRDIHPGDRVACYGV 83 ++ K+ G P+S LGYS+ G V ++ I GDRVAC G Sbjct: 71 MKVINKAKQDG--PISAFQQAMGRLEKREPLGYSSAGTVISVSEDIASIKVGDRVACAGA 128 Query: 84 PYVRHAEVISVPTNLVTKVPHHVNPEEAAFTGLGAIAIHALRTADVRFXXXXXXXXXXXX 143 Y HA+V VP NL KVP +V+ + A FT +G+IA+ +R ADVR Sbjct: 129 GYANHADVAFVPKNLCVKVPDNVDLKNACFTTVGSIAMQGVRNADVRVGENVVVVGLGLI 188 Query: 144 XXXXAQIASAAACHTAAYDLSEDRVQLLQGQMGITSGFS-SEEEVEHFVMNETGGAGFDS 202 QI A+ C DL E +V+L + ++G S + +E V + G G D Sbjct: 189 GLITVQILKASGCRVFGIDLDESKVKLAK-ELGADFALSRNTPNIEESVRQFSRGIGADV 247 Query: 203 ILLCAGGPGEVLINKSLEWLRDLGKIVIVGDLSMEFSRDLMFRKEAQILISRAGGPGRYD 262 ++ A ++ + + R+ GK+VIVG + M+ R+ + KE ++ +SR+ GPGRYD Sbjct: 248 TIIAAATRSNDPVDFAGKITRERGKVVIVGLVGMDIPREEYYNKEIELRVSRSYGPGRYD 307 Query: 263 MRYEEDNQDYPIGFVRWTEGRNMDEYVRLLAEKRITVSPAITHRFALEEAPAAYGIYQ 320 YEE +DYP +VRWTE RNM ++ LL+ K+I++ P I+H FA+E+AP AY I + Sbjct: 308 RAYEEFGRDYPAPYVRWTENRNMQAFLDLLSMKKISMDPIISHEFAIEQAPDAYDIIE 365 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.135 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,931,858,187 Number of extensions: 78067452 Number of successful extensions: 188271 Number of sequences better than 1.0e-05: 217 Number of HSP's gapped: 188584 Number of HSP's successfully gapped: 217 Length of query: 335 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 199 Effective length of database: 1,779,438,163 Effective search space: 354108194437 Effective search space used: 354108194437 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)