BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0566.0029.p (339 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A6LYK8.1 A6LYK8_CLOB8 Short-chain dehydrogenase/reductase SDR 447 e-124 C2UXV3.1 C2UXV3_BACCE Short-chain dehydrogenase/reductase SDR 403 e-110 B5ZVC2.1 B5ZVC2_RHILW Short-chain dehydrogenase/reductase SDR 401 e-110 B0IZ86.1 B0IZ86_RHILT Short-chain dehydrogenase/reductase SDR 397 e-109 Q1MJH8.1 Q1MJH8_RHIL3 Putative short-chain dehydrogenase/oxidore... 397 e-109 >A6LYK8.1 A6LYK8_CLOB8 Short-chain dehydrogenase/reductase SDR Length = 327 Score = 447 bits (1151), Expect = e-124, Method: Compositional matrix adjust. Identities = 213/330 (64%), Positives = 257/330 (77%), Gaps = 6/330 (1%) Query: 10 TVQAPLASGFDARTTAAEVIGGLDLTGKVAIVTGGYSGIGLETSRVLAEAGATVIVPART 69 T QAP+ S ++ TTA +VI G++L GK+AIVTGGYSGIG+ET++VLAEAGATVI+PAR Sbjct: 4 TTQAPINSKYNFFTTAKDVIDGINLKGKIAIVTGGYSGIGMETAKVLAEAGATVIIPARD 63 Query: 70 PAKAKAAVSGIPRLELETLDLMDPASIDSFARRFIASGRPLHFLINSAGIMAAPLARDAR 129 KAK A+ IP +E+E LDLMDP SIDSFA++FI S R LH LINSAGIMA PL RD R Sbjct: 64 IEKAKGAMDNIPNIEIEHLDLMDPMSIDSFAQKFINSQRSLHILINSAGIMAPPLIRDKR 123 Query: 130 GYESQFATNHLGHFQLTARLWPALRQADQARVISVSSRAHRLGGVDLSDPNFERREYDKW 189 GYESQFATNHLGHFQLTARLWPAL+ A ARVISVSSRA RLGGV+ DPNF++ EYD W Sbjct: 124 GYESQFATNHLGHFQLTARLWPALKNAKSARVISVSSRAQRLGGVNFDDPNFQKTEYDSW 183 Query: 190 KSYAQSKSANVLFAVALDEKAKVYGVRAFAVHPGLIPSSGIGRFLKTEEVGVKPVKVQPA 249 K+YAQSKSAN LFAV LD K +GVRAF+VHPGLIP++ +GRF + V+ +K Sbjct: 184 KAYAQSKSANSLFAVELDRLGKTHGVRAFSVHPGLIPTTNLGRFSVNGKTTVQELKTNTR 243 Query: 250 QEDEKGKPASNTNSDFMKNTEQGAATSVWCAVSEQLAGLGGVYCEDADIAEAVPADSVQG 309 ++D +NT S+ K EQGAATSVWCA + L G+GGVYCED +IAEAVP DS++ Sbjct: 244 KDD------TNTKSNEFKTIEQGAATSVWCATNSILDGMGGVYCEDCNIAEAVPYDSLKD 297 Query: 310 SGVWPWAIDREAAERLWVLSEQLTGVKFTI 339 +GV PWAID+ A++LW+LSE+LT VKF I Sbjct: 298 NGVRPWAIDKNLAKKLWILSEELTNVKFII 327 >C2UXV3.1 C2UXV3_BACCE Short-chain dehydrogenase/reductase SDR Length = 333 Score = 403 bits (1035), Expect = e-110, Method: Compositional matrix adjust. Identities = 202/328 (61%), Positives = 239/328 (72%), Gaps = 7/328 (2%) Query: 10 TVQAPLASGFDARTTAAEVIGGLDLTGKVAIVTGGYSGIGLETSRVLAEAGATVIVPART 69 T+Q P+ SGF ARTTA E +GG DL+GK+AIVTGGYSG+GLE +RVLAEAGATVIVPAR Sbjct: 9 TIQKPIYSGFGARTTAKEALGGKDLSGKIAIVTGGYSGLGLEITRVLAEAGATVIVPARR 68 Query: 70 PAKAKAAVSGIPRLELETLDLMDPASIDSFARRFIASGRPLHFLINSAGIMAAPLARDAR 129 +A+ A+S I R+ELE LDLMDP SID+FA RF+ + RPLH L+N AGIMA PL RDAR Sbjct: 69 IEQAQEAISNIARVELEKLDLMDPNSIDAFANRFLNTDRPLHILVNGAGIMANPLKRDAR 128 Query: 130 GYESQFATNHLGHFQLTARLWPALRQADQARVISVSSRAHRLGGVDLSDPNFERREYDKW 189 GYESQFATNHLGHFQLTARLWPAL QA+ ARV+S SSR HR+ G+D +DPNF R YDKW Sbjct: 129 GYESQFATNHLGHFQLTARLWPALVQANGARVVSASSRGHRICGIDFNDPNFTNRHYDKW 188 Query: 190 KSYAQSKSANVLFAVALDEKAKVYGVRAFAVHPGLIPSSGIGRFLKTEEVGVKPVKVQPA 249 ++Y QSK+AN LFA LD++ K + VRAF+ HPG I +S + R L EE+ Sbjct: 189 QAYGQSKTANALFAAELDKRGKEFHVRAFSAHPGTIVTS-LSRNLTDEEM------RSMG 241 Query: 250 QEDEKGKPASNTNSDFMKNTEQGAATSVWCAVSEQLAGLGGVYCEDADIAEAVPADSVQG 309 DE G A + K+ +GAAT VWCAV+ QL GGVYCE+ DIAEAV AD+ Sbjct: 242 ALDEHGNRALVKYDEECKSIPEGAATIVWCAVNNQLDNKGGVYCENVDIAEAVSADNPDP 301 Query: 310 SGVWPWAIDREAAERLWVLSEQLTGVKF 337 SGV PWA + E A LW LSE LTGV F Sbjct: 302 SGVRPWACNTEYAAMLWELSETLTGVNF 329 >B5ZVC2.1 B5ZVC2_RHILW Short-chain dehydrogenase/reductase SDR Length = 324 Score = 401 bits (1031), Expect = e-110, Method: Compositional matrix adjust. Identities = 205/330 (62%), Positives = 239/330 (72%), Gaps = 17/330 (5%) Query: 10 TVQAPLASGFDARTTAAEVIGGLDLTGKVAIVTGGYSGIGLETSRVLAEAGATVIVPART 69 T QAP+ SGF A +TAAEVI G DL+GKVAIVTGGYSG+G ET+RVLAEAGA VIVPAR Sbjct: 3 TPQAPIVSGFGAASTAAEVIAGHDLSGKVAIVTGGYSGLGFETARVLAEAGAKVIVPARN 62 Query: 70 PAKAKAAVSGIPRLELETLDLMDPASIDSFARRFIASGRPLHFLINSAGIMAAPLARDAR 129 KAKAAV IP L LE LDLMDP SID FA RF+ SG PLH LIN+A +MA PL RDAR Sbjct: 63 LGKAKAAVENIPGLALEMLDLMDPGSIDDFADRFLESGAPLHLLINNAAVMANPLTRDAR 122 Query: 130 GYESQFATNHLGHFQLTARLWPALRQADQARVISVSSRAHRLGGVDLSDPNFERREYDKW 189 GYESQF+TNHLGHFQL ARLWPAL +A+ ARV++VSSR H GVD DPNFE REY + Sbjct: 123 GYESQFSTNHLGHFQLAARLWPALVKAEGARVVAVSSRGHVFSGVDFDDPNFENREYAPY 182 Query: 190 KSYAQSKSANVLFAVALDEKAKVYGVRAFAVHPGLIPSSGI-----GRFLKTEEVGVKPV 244 +Y QSK+AN LFAV+LD YG+RAF++HPG I ++ + G FLK Sbjct: 183 LAYGQSKTANALFAVSLDAVGAKYGIRAFSLHPGGIVTTNLVRHQSGEFLKAS------- 235 Query: 245 KVQPAQEDEKGKPASNTNSDFMKNTEQGAATSVWCAVSEQLAGLGGVYCEDADIAEAVPA 304 DE G+P + ++ K EQGA+T+VWCAVSE+L GLGGVYCE+ +IA AVP Sbjct: 236 ----GYVDEHGQPVIDPENN-KKTVEQGASTTVWCAVSEKLDGLGGVYCENCNIATAVPG 290 Query: 305 DSVQGSGVWPWAIDREAAERLWVLSEQLTG 334 DS + GV PWA D E AERLW LSE+LTG Sbjct: 291 DSTEMLGVRPWATDPELAERLWQLSERLTG 320 >B0IZ86.1 B0IZ86_RHILT Short-chain dehydrogenase/reductase SDR Length = 324 Score = 397 bits (1021), Expect = e-109, Method: Compositional matrix adjust. Identities = 205/330 (62%), Positives = 240/330 (72%), Gaps = 17/330 (5%) Query: 10 TVQAPLASGFDARTTAAEVIGGLDLTGKVAIVTGGYSGIGLETSRVLAEAGATVIVPART 69 T QAP+ SGF A +TA +VI G DL+GKVAIVTGGYSG+GLET+RVLAEAGA V+VPAR Sbjct: 3 TPQAPIGSGFGAASTATDVIAGHDLSGKVAIVTGGYSGLGLETARVLAEAGARVVVPARN 62 Query: 70 PAKAKAAVSGIPRLELETLDLMDPASIDSFARRFIASGRPLHFLINSAGIMAAPLARDAR 129 KAKAAV IP L LE L+LMDP SID FA RF+ S PLH LIN+A +MA PL RDAR Sbjct: 63 LEKAKAAVESIPGLALEKLNLMDPGSIDDFADRFLESVEPLHLLINNAAVMANPLTRDAR 122 Query: 130 GYESQFATNHLGHFQLTARLWPALRQADQARVISVSSRAHRLGGVDLSDPNFERREYDKW 189 GYESQF+TNHLGHFQLTARLWPAL +A+ ARV++VSSR H GVD DPNFE REY + Sbjct: 123 GYESQFSTNHLGHFQLTARLWPALVKAEGARVVAVSSRGHVFSGVDFDDPNFENREYAPY 182 Query: 190 KSYAQSKSANVLFAVALDEKAKVYGVRAFAVHPGLIPSSGIGR-----FLKTEEVGVKPV 244 +Y QSK+AN LFA++LD +GVRAF++HPG I ++ + R FLKT Sbjct: 183 LAYGQSKTANALFAISLDALGAKHGVRAFSLHPGGIVTTDLVRHQSSDFLKTS------- 235 Query: 245 KVQPAQEDEKGKPASNTNSDFMKNTEQGAATSVWCAVSEQLAGLGGVYCEDADIAEAVPA 304 DE GKP + ++ K EQGAAT+VWCAVSE+L GLGGVYCE+ DIA AVP Sbjct: 236 ----GYVDEDGKPVIDPQNN-KKTIEQGAATTVWCAVSEKLEGLGGVYCENCDIATAVPG 290 Query: 305 DSVQGSGVWPWAIDREAAERLWVLSEQLTG 334 DS + GV PWA D E AERLW LSE+LTG Sbjct: 291 DSTEMLGVRPWATDPELAERLWQLSERLTG 320 >Q1MJH8.1 Q1MJH8_RHIL3 Putative short-chain dehydrogenase/oxidoreductase Length = 324 Score = 397 bits (1020), Expect = e-109, Method: Compositional matrix adjust. Identities = 202/325 (62%), Positives = 239/325 (73%), Gaps = 7/325 (2%) Query: 10 TVQAPLASGFDARTTAAEVIGGLDLTGKVAIVTGGYSGIGLETSRVLAEAGATVIVPART 69 T QAP+ SGF A +TAA+VI G DL GKVAIVTGGYSG+G ET+RVLAEAGA V+VPAR Sbjct: 3 TPQAPIGSGFGAASTAADVIAGHDLLGKVAIVTGGYSGLGRETARVLAEAGARVVVPARN 62 Query: 70 PAKAKAAVSGIPRLELETLDLMDPASIDSFARRFIASGRPLHFLINSAGIMAAPLARDAR 129 KAKAAV IP L +E LDLMDP SID FA RF+ SG PLH LIN+A +MA PL RDAR Sbjct: 63 LGKAKAAVESIPGLAVEKLDLMDPGSIDDFADRFLESGEPLHLLINNAAVMANPLTRDAR 122 Query: 130 GYESQFATNHLGHFQLTARLWPALRQADQARVISVSSRAHRLGGVDLSDPNFERREYDKW 189 GYESQF+TNHLGHFQLTARLWPAL +A+ ARVI+VSSR H GVD DPNFE REY + Sbjct: 123 GYESQFSTNHLGHFQLTARLWPALVKAEGARVIAVSSRGHVFSGVDFDDPNFENREYAPY 182 Query: 190 KSYAQSKSANVLFAVALDEKAKVYGVRAFAVHPGLIPSSGIGRFLKTEEVGVKPVKVQPA 249 +Y QSK+AN LFA++LD +GVRAF++HPG I ++ + R ++ + Sbjct: 183 LAYGQSKTANALFAISLDALGAKHGVRAFSLHPGGIVTTNLVRHQSSDFLKAS------G 236 Query: 250 QEDEKGKPASNTNSDFMKNTEQGAATSVWCAVSEQLAGLGGVYCEDADIAEAVPADSVQG 309 DE GKP + ++ K EQGA+T+VWCAVSE+L GLGGVYCE+ DIA AVP DS + Sbjct: 237 YVDEDGKPVIDPENN-KKTIEQGASTTVWCAVSEKLEGLGGVYCENCDIAAAVPGDSTEM 295 Query: 310 SGVWPWAIDREAAERLWVLSEQLTG 334 GV PWA D E AERLW LSE+LTG Sbjct: 296 LGVRPWATDPEFAERLWQLSERLTG 320 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.131 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,914,813,693 Number of extensions: 78795735 Number of successful extensions: 298691 Number of sequences better than 1.0e-05: 5068 Number of HSP's gapped: 300721 Number of HSP's successfully gapped: 5075 Length of query: 339 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 203 Effective length of database: 1,779,438,163 Effective search space: 361225947089 Effective search space used: 361225947089 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)