BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0566.0013.p (304 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KK62.1 B3KK62_9BACI NAD-dependent epimerase/dehydratase 300 2e-79 B8DFI0.1 B8DFI0_LISMH NAD dependent epimerase/dehydratase family 289 2e-76 Q8Y6E3.1 Q8Y6E3_LISMO Lmo1744 protein 287 7e-76 Q4ET42.1 Q4ET42_LISMO Putative uncharacterized protein 287 7e-76 Q71YS4.1 Q71YS4_LISMF Putative uncharacterized protein 287 1e-75 >B3KK62.1 B3KK62_9BACI NAD-dependent epimerase/dehydratase Length = 295 Score = 300 bits (767), Expect = 2e-79, Method: Compositional matrix adjust. Identities = 153/292 (52%), Positives = 199/292 (68%), Gaps = 1/292 (0%) Query: 6 NILVLGGTRFFGKRLVERLFQDADTQVTILTRGITKDGFGSRVNRLNVDRTDAKALAEAV 65 ILVLGGTRFFGKRLV RL + D VTILTRG +D FG V+RL DR D ++L +AV Sbjct: 3 KILVLGGTRFFGKRLVNRLVANGDA-VTILTRGQHQDPFGGAVSRLAADRKDTESLKQAV 61 Query: 66 RDTVWDVVYDNICFSPDEAAAASRIFAETAKRYILTSSLSVYDPGPGILREADFDPLSFP 125 +D+VYDNIC++P+EA A+ +FA +Y+LTS+LSVYD +RE DFDP +P Sbjct: 62 GSQDFDIVYDNICYTPEEAGQAADLFAGRVGQYVLTSTLSVYDFADHPVREEDFDPYRYP 121 Query: 126 LRLGAKEDFNYQEGKRLAETVLLTQAAFPAAAVRFPIVLGTDDYTKRLHFHIEHVLEGKA 185 + + D++Y+EGKR AE VL + AVR PIVLG DDYT+RL FHIEH G+A Sbjct: 122 VMMNESRDYSYKEGKRQAEAVLFGRHNLNVTAVRLPIVLGPDDYTRRLLFHIEHAASGEA 181 Query: 186 IGVPNPGALISFIRSDEAADFLYGLGFSQLAGPVNACSDGTLAIGDILNIIGQKTGREAV 245 IG+P+P A ISF+ ++EAA+FL G QL GPVNACS GT+++ +++ +I Q+ GR A Sbjct: 182 IGLPDPDAQISFVHAEEAAEFLEWAGRVQLEGPVNACSSGTISVREMMGLIQQEIGRTAP 241 Query: 246 VLSETADQDMSPFGIEESWYMDTAKARGAGFQFLKLAEWFPELIGELATELN 297 + S A MSPFGI W MD AKA AGF+F L +W P+LI EL+ +L Sbjct: 242 LQSSAAPDHMSPFGIPGDWTMDHAKAAAAGFKFWDLHDWMPKLIAELSADLK 293 >B8DFI0.1 B8DFI0_LISMH NAD dependent epimerase/dehydratase family Length = 291 Score = 289 bits (740), Expect = 2e-76, Method: Compositional matrix adjust. Identities = 153/291 (52%), Positives = 190/291 (65%), Gaps = 3/291 (1%) Query: 6 NILVLGGTRFFGKRLVERLFQDADTQVTILTRGITKDGFGSRVNRLNVDRTDAKALAEAV 65 ILV GGTRFFGK+LVERL + VTI TRG T+D FG V R+ ++R AL + Sbjct: 2 KILVFGGTRFFGKKLVERLISEGH-DVTIGTRGKTEDHFGDAVKRVVLNRESRDALFQLA 60 Query: 66 RDTVWDVVYDNICFSPDEAAAASRIFAETAKRYILTSSLSVYDPGPGILREADFDPLSFP 125 ++ WDV+YDNICFSP EA A F KRYI TSSLSVY L E DF+P + Sbjct: 61 KEE-WDVIYDNICFSPKEALYAVDAFKGKVKRYIYTSSLSVYSQKGRALVEDDFNPEHYE 119 Query: 126 LRLGAKEDFNYQEGKRLAETVLLTQAAFPAAAVRFPIVLGTDDYTKRLHFHIEHVLEGKA 185 + +G KEDF+Y EGKRLAE V +A+FP AVRFPIVLG DDYTKRLHFHIEH+ + Sbjct: 120 IVIGDKEDFDYGEGKRLAEAVFFQKASFPVVAVRFPIVLGLDDYTKRLHFHIEHIKNHQE 179 Query: 186 IGVPNPGALISFIRSDEAADFLYGLGF-SQLAGPVNACSDGTLAIGDILNIIGQKTGREA 244 IG+ N A I FI SDEAA FL +G S + GP+NA S+GT A+ + ++ KTG A Sbjct: 180 IGISNGQAEIGFITSDEAAQFLAWVGVESDVTGPINAASNGTYALNGFIKMLEAKTGELA 239 Query: 245 VVLSETADQDMSPFGIEESWYMDTAKARGAGFQFLKLAEWFPELIGELATE 295 +V T D D SPFGIE+++Y+D KA AGF F +L EW P L+ ++ E Sbjct: 240 LVEEVTDDADDSPFGIEKTYYLDNTKATEAGFVFKELKEWLPALVTDILKE 290 >Q8Y6E3.1 Q8Y6E3_LISMO Lmo1744 protein Length = 291 Score = 287 bits (735), Expect = 7e-76, Method: Compositional matrix adjust. Identities = 154/290 (53%), Positives = 189/290 (65%), Gaps = 3/290 (1%) Query: 7 ILVLGGTRFFGKRLVERLFQDADTQVTILTRGITKDGFGSRVNRLNVDRTDAKALAEAVR 66 ILV GGTRFFGK+LVERL + VTI TRG T+D FG V R+ ++R AL + + Sbjct: 3 ILVFGGTRFFGKKLVERLVSEGH-DVTIGTRGKTEDNFGDTVKRVVLNRESRDALFQLAK 61 Query: 67 DTVWDVVYDNICFSPDEAAAASRIFAETAKRYILTSSLSVYDPGPGILREADFDPLSFPL 126 + WDV+YDNICFSP EA A F KRYI TSSLSVY L EADF+P + + Sbjct: 62 ED-WDVIYDNICFSPKEALYAVDAFKGKVKRYIYTSSLSVYSQKGRALVEADFNPEHYEI 120 Query: 127 RLGAKEDFNYQEGKRLAETVLLTQAAFPAAAVRFPIVLGTDDYTKRLHFHIEHVLEGKAI 186 +G KEDF+Y EGKRLAE V +A+FP AVRFPIVLG DDYTKRLHFHI H+ + I Sbjct: 121 VIGDKEDFDYGEGKRLAEAVFFQKASFPVVAVRFPIVLGLDDYTKRLHFHINHIKNHQEI 180 Query: 187 GVPNPGALISFIRSDEAADFLYGLGF-SQLAGPVNACSDGTLAIGDILNIIGQKTGREAV 245 G+ N A I FI SDEAA FL +G S L GPVNA S+GT A+ + ++ +K G A+ Sbjct: 181 GISNGQAEIGFITSDEAAHFLEWVGVESDLTGPVNATSNGTYALNGFIKMLEEKIGELAL 240 Query: 246 VLSETADQDMSPFGIEESWYMDTAKARGAGFQFLKLAEWFPELIGELATE 295 V T D D SPFGIE+++Y+D AKA AGF F L W P L+ ++ E Sbjct: 241 VEEVTDDVDDSPFGIEKTYYLDNAKATEAGFVFNDLKVWLPALVTDILKE 290 >Q4ET42.1 Q4ET42_LISMO Putative uncharacterized protein Length = 291 Score = 287 bits (735), Expect = 7e-76, Method: Compositional matrix adjust. Identities = 154/290 (53%), Positives = 189/290 (65%), Gaps = 3/290 (1%) Query: 7 ILVLGGTRFFGKRLVERLFQDADTQVTILTRGITKDGFGSRVNRLNVDRTDAKALAEAVR 66 ILV GGTRFFGK+LVERL + VTI TRG T+D FG V R+ ++R AL + + Sbjct: 3 ILVFGGTRFFGKKLVERLVSEGH-DVTIGTRGKTEDNFGDTVKRVVLNRESRDALFQLAK 61 Query: 67 DTVWDVVYDNICFSPDEAAAASRIFAETAKRYILTSSLSVYDPGPGILREADFDPLSFPL 126 + WDV+YDNICFSP EA A F KRYI TSSLSVY L EADF+P + + Sbjct: 62 ED-WDVIYDNICFSPKEALYAVDAFKGKVKRYIYTSSLSVYSQKGRALVEADFNPEHYEI 120 Query: 127 RLGAKEDFNYQEGKRLAETVLLTQAAFPAAAVRFPIVLGTDDYTKRLHFHIEHVLEGKAI 186 +G KEDF+Y EGKRLAE V +A+FP AVRFPIVLG DDYTKRLHFHI H+ + I Sbjct: 121 VIGDKEDFDYGEGKRLAEAVFFQKASFPVVAVRFPIVLGLDDYTKRLHFHINHIKNHQEI 180 Query: 187 GVPNPGALISFIRSDEAADFLYGLGF-SQLAGPVNACSDGTLAIGDILNIIGQKTGREAV 245 G+ N A I FI SDEAA FL +G S L GPVNA S+GT A+ + ++ +K G A+ Sbjct: 181 GISNGQAEIGFITSDEAAHFLEWVGVESDLTGPVNATSNGTYALNGFIKMLEEKIGELAL 240 Query: 246 VLSETADQDMSPFGIEESWYMDTAKARGAGFQFLKLAEWFPELIGELATE 295 V T D D SPFGIE+++Y+D AKA AGF F L W P L+ ++ E Sbjct: 241 VEEVTDDVDDSPFGIEKTYYLDNAKATEAGFVFNDLKVWLPALVTDILKE 290 >Q71YS4.1 Q71YS4_LISMF Putative uncharacterized protein Length = 291 Score = 287 bits (734), Expect = 1e-75, Method: Compositional matrix adjust. Identities = 153/290 (52%), Positives = 189/290 (65%), Gaps = 3/290 (1%) Query: 7 ILVLGGTRFFGKRLVERLFQDADTQVTILTRGITKDGFGSRVNRLNVDRTDAKALAEAVR 66 ILV GGTRFFGK+LVERL + +VTI TRG T+D FG V R+ ++R AL + + Sbjct: 3 ILVFGGTRFFGKKLVERLVSEGH-EVTIGTRGKTEDNFGDTVKRVILNRESRDALFQLAK 61 Query: 67 DTVWDVVYDNICFSPDEAAAASRIFAETAKRYILTSSLSVYDPGPGILREADFDPLSFPL 126 + WDV+YDNICFSP EA A F KRYI TSSLSVY L EADF+P + + Sbjct: 62 ED-WDVIYDNICFSPKEALYAVDAFKGKVKRYIYTSSLSVYSQKGRALVEADFNPEHYEI 120 Query: 127 RLGAKEDFNYQEGKRLAETVLLTQAAFPAAAVRFPIVLGTDDYTKRLHFHIEHVLEGKAI 186 +G KEDF+Y EGKRLAE V +A+FP AVRFPIVLG DDYTKRLHFHI H+ + I Sbjct: 121 VIGDKEDFDYGEGKRLAEAVFFQKASFPVVAVRFPIVLGLDDYTKRLHFHINHIKNHQEI 180 Query: 187 GVPNPGALISFIRSDEAADFLYGLGF-SQLAGPVNACSDGTLAIGDILNIIGQKTGREAV 245 G+ N A I FI SDEAA FL +G S L GPVNA S+GT A+ + ++ +K G+ A+ Sbjct: 181 GISNGQAEIGFITSDEAAHFLEWVGVESDLTGPVNATSNGTYALNGFIKMLEEKIGKLAL 240 Query: 246 VLSETADQDMSPFGIEESWYMDTAKARGAGFQFLKLAEWFPELIGELATE 295 V T D D SPFGIE+++Y+D KA AGF F L W P L+ + E Sbjct: 241 VEEVTDDVDDSPFGIEKTYYLDNTKATEAGFVFNDLKVWLPALVTAILKE 290 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.138 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,797,219,474 Number of extensions: 73797930 Number of successful extensions: 169970 Number of sequences better than 1.0e-05: 249 Number of HSP's gapped: 169715 Number of HSP's successfully gapped: 249 Length of query: 304 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 169 Effective length of database: 1,788,859,178 Effective search space: 302317201082 Effective search space used: 302317201082 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)