BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0565.0017.p (287 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1D6N6.1 B1D6N6_9BACL Short-chain dehydrogenase/reductase SDR 329 2e-88 B3KBT8.1 B3KBT8_9BACI Short-chain dehydrogenase/reductase SDR 312 2e-83 B3K8G2.1 B3K8G2_9BACI Short-chain dehydrogenase/reductase SDR 309 2e-82 A9B027.1 A9B027_HERA2 Short-chain dehydrogenase/reductase SDR 271 6e-71 Q5WC36.1 Q5WC36_BACSK Putative uncharacterized protein 266 2e-69 >B1D6N6.1 B1D6N6_9BACL Short-chain dehydrogenase/reductase SDR Length = 285 Score = 329 bits (843), Expect = 2e-88, Method: Compositional matrix adjust. Identities = 161/277 (58%), Positives = 199/277 (71%), Gaps = 1/277 (0%) Query: 5 QQHRGTALITGANNGIGLELTRKMLAEGWQIIALIRSSFPGDDSLVSGAVKSKQLRIYK- 63 + L+TGA+ GIGLELTRK+L+E WQ++AL RS FP DD+ + A+ LR+YK Sbjct: 4 NKRENIVLVTGASAGIGLELTRKLLSEDWQVVALNRSDFPADDTGIQKAIMEGWLRVYKT 63 Query: 64 ADLSDFTALKRALNEIRLQEQQIDLLFNNAGGSFPELLYSKQGRELHFELQTVVPYIIIM 123 ADL+D+ +L+R L EI+ +EQ+ID+LFNNAGGSFPEL +SKQGRE H+EL TVVPYII+M Sbjct: 64 ADLTDYASLRRTLEEIKGKEQRIDILFNNAGGSFPELSHSKQGREKHYELLTVVPYIILM 123 Query: 124 XXXXXXXXXXXXTVVNTSSNAAMTVRHFDPETLEHPTKFKKLLGPYAASKLALSLWTREL 183 TV+NTSS+A V+ F+ E LEHP F+KLLGPYA SKLALSLWT+ + Sbjct: 124 ELKELIKNGSLKTVINTSSSALNYVKEFNIEILEHPKTFRKLLGPYATSKLALSLWTQAI 183 Query: 184 APQLAAAGIMIRSADPGANNTLRSGKDSGLPFWLKPVMKLFFPHPSHGAGLLYNAALGRH 243 APQLA GI IRS DPG+NNTLR GK SGLP +K +MKLFF P+HGA LY ALG H Sbjct: 184 APQLAKEGIKIRSVDPGSNNTLRKGKKSGLPLVIKWLMKLFFSPPTHGANQLYEGALGEH 243 Query: 244 SKLPGVFLIKDQITELKFAEYSSRVLSKVQAIYEQEF 280 GVFL+K Q ELKF ++S VL ++ IYE EF Sbjct: 244 RNKTGVFLLKGQTAELKFNDHSRNVLERIHEIYEHEF 280 >B3KBT8.1 B3KBT8_9BACI Short-chain dehydrogenase/reductase SDR Length = 289 Score = 312 bits (800), Expect = 2e-83, Method: Compositional matrix adjust. Identities = 155/277 (55%), Positives = 190/277 (68%), Gaps = 1/277 (0%) Query: 5 QQHRGTALITGANNGIGLELTRKMLAEGWQIIALIRSSFPGDDSLVSGAVKSKQLRIYKA 64 Q ALITGA+NGIGL LTRKML+E WQ++AL RS FP DD + +K +LR YK Sbjct: 4 NQREHIALITGASNGIGLALTRKMLSENWQVVALNRSDFPADDRFLQNHLKDGRLRAYKV 63 Query: 65 -DLSDFTALKRALNEIRLQEQQIDLLFNNAGGSFPELLYSKQGRELHFELQTVVPYIIIM 123 DL+D+ +L+ +L EI+ +EQ+ID+LFNNAGG EL YSKQGRELH+EL TVVP+II+M Sbjct: 64 KDLTDYGSLRHSLEEIKSKEQRIDILFNNAGGGLSELRYSKQGRELHYELLTVVPFIILM 123 Query: 124 XXXXXXXXXXXXTVVNTSSNAAMTVRHFDPETLEHPTKFKKLLGPYAASKLALSLWTREL 183 TV+NTSS + F E LEHP F+K+ GPYA SKLALSLWT+ + Sbjct: 124 ELKELLKNGSLKTVINTSSQVFRFTKEFTIEKLEHPKTFRKMYGPYATSKLALSLWTQAV 183 Query: 184 APQLAAAGIMIRSADPGANNTLRSGKDSGLPFWLKPVMKLFFPHPSHGAGLLYNAALGRH 243 APQLA GI IRS DPG NNTLR GKDSGL + MK F P+HGA LLY ALG++ Sbjct: 184 APQLAKEGIKIRSVDPGINNTLRKGKDSGLTAGFELFMKFFSSPPTHGANLLYEGALGKN 243 Query: 244 SKLPGVFLIKDQITELKFAEYSSRVLSKVQAIYEQEF 280 K GVFL KD+I +LKF E++ RVL K+ IY E+ Sbjct: 244 RKETGVFLFKDRIADLKFTEHAQRVLEKISDIYSLEY 280 >B3K8G2.1 B3K8G2_9BACI Short-chain dehydrogenase/reductase SDR Length = 282 Score = 309 bits (791), Expect = 2e-82, Method: Compositional matrix adjust. Identities = 152/277 (54%), Positives = 201/277 (72%), Gaps = 1/277 (0%) Query: 5 QQHRGTALITGANNGIGLELTRKMLAEGWQIIALIRSSFPGDDSLVSGAVKSKQLRIYKA 64 ++ ALITGA++GIGLELT+++L+EGWQ++ALIRS+FP DD+ + A++ +QLR YKA Sbjct: 4 KRMNNVALITGASSGIGLELTKRLLSEGWQVLALIRSAFPTDDARIQDALRLRQLRTYKA 63 Query: 65 DLSDFTALKRALNEIRLQEQQIDLLFNNAGGSFPELLYSKQGRELHFELQTVVPYIIIMX 124 DLSDFT L+ +L++I+ E IDLLFNNAG SF EL YS+QGRE+HFE+ +VVPYII+M Sbjct: 64 DLSDFTDLQASLDQIKKYEGHIDLLFNNAGVSFGELSYSQQGREMHFEVNSVVPYIILME 123 Query: 125 XXXXXXXXXXXTVVNTSSNAAMTVRHFDPETLEHPTKFKKLLGPYAASKLALSLWTRELA 184 TV+NTSSNA + ++ FD TL P FKKL+GPYA SKLALSLWT+E+A Sbjct: 124 LKELLGKGSFKTVINTSSNALLYLKQFDHRTLASPVGFKKLIGPYARSKLALSLWTQEIA 183 Query: 185 PQLAAAGIMIRSADPGANNTLRSGKDSGLPFWLKPVMKLFFPHPSHGAGLLYNAALGRHS 244 P + A GI IRS PG N T + K++G+P ++ P+ LFF HPS GA LY AALGR Sbjct: 184 PSMQADGITIRSVCPGGNKTTMT-KNAGMPKFMIPIRNLFFSHPSEGASRLYEAALGRSR 242 Query: 245 KLPGVFLIKDQITELKFAEYSSRVLSKVQAIYEQEFA 281 + GVFL K + T LKF+ + +LS ++ IY+QEFA Sbjct: 243 QSTGVFLNKGKATPLKFSSQGAELLSTIELIYKQEFA 279 >A9B027.1 A9B027_HERA2 Short-chain dehydrogenase/reductase SDR Length = 280 Score = 271 bits (692), Expect = 6e-71, Method: Compositional matrix adjust. Identities = 140/273 (51%), Positives = 184/273 (67%), Gaps = 3/273 (1%) Query: 10 TALITGANNGIGLELTRKMLAEGWQIIALIRSSFPGDDSLVSGAVKSKQLRIYKADLSDF 69 TALITGAN+GIGLELT+++L EGW +I LIRS FP ++ ++ A + +LR Y+A++SDF Sbjct: 9 TALITGANSGIGLELTKRLLGEGWAVIGLIRSQFPTNEPILQAAQTAGKLRSYQAEISDF 68 Query: 70 TALKRALNEIRLQEQQIDLLFNNAGGSFPELLYSKQGRELHFELQTVVPYIIIMXXXXXX 129 T L+ AL I+ QE +DLLFNNAG + +L++S QGREL FELQ+VVPYII M Sbjct: 69 TQLRAALQTIKQQETSLDLLFNNAGRATEQLVFSPQGRELSFELQSVVPYIIAMELKELL 128 Query: 130 XXXXXXTVVNTSSNAAMTVRHFDPETLEHPTKFKKLLGPYAASKLALSLWTRELAPQLAA 189 T++NTSSN + +R + LE P FKKL GPYA SKLALSLW + LAPQL Sbjct: 129 LRGQNKTIINTSSNVLLALRKLEIAQLERPQSFKKLFGPYATSKLALSLWNQALAPQLEK 188 Query: 190 AGIMIRSADPGANN-TLRSGKDSGLPFWLKPVMKLFFPHPSHGAGLLYNAALGRHSKLPG 248 GI + S PG N +L++ ++GLP+WL+P L F HPS+GA LYN A + G Sbjct: 189 EGIKLYSVCPGGNRGSLKT--NNGLPWWLRPFHGLLFKHPSNGAERLYNTAFNQQQFPSG 246 Query: 249 VFLIKDQITELKFAEYSSRVLSKVQAIYEQEFA 281 F+ K + T L A+ + VL+KVQAIY+QEFA Sbjct: 247 SFINKGKPTALPAAQQAHAVLNKVQAIYQQEFA 279 >Q5WC36.1 Q5WC36_BACSK Putative uncharacterized protein Length = 278 Score = 266 bits (679), Expect = 2e-69, Method: Compositional matrix adjust. Identities = 133/277 (48%), Positives = 190/277 (68%), Gaps = 4/277 (1%) Query: 5 QQHRGTALITGANNGIGLELTRKMLAEGWQIIALIRSSFPGDDSLVSGAVKSKQLRIYKA 64 ++H LITGAN+GIGLELT+ +L +GW++ ALIRS FP + ++ A+K LR+YKA Sbjct: 2 KEHNKVVLITGANSGIGLELTKNLLLQGWEVAALIRSDFPNEQPIIIDALKKNHLRVYKA 61 Query: 65 DLSDFTALKRALNEIRLQEQQIDLLFNNAGGSFPELLYSKQGRELHFELQTVVPYIIIMX 124 DL+DF++L+ AL+EI+ E +ID+LFNNAG SF E +SKQGRELHFE+ T+VP+II M Sbjct: 62 DLADFSSLRNALDEIKKSETKIDMLFNNAGVSFAEYKHSKQGRELHFEVNTLVPFIITME 121 Query: 125 XXXXXXXXXXXTVVNTSSNAAMTVRHFDPETLEHP-TKFKKLLGPYAASKLALSLWTREL 183 T++NTSSN+ + ++ FD T+ +P T FKKL+GPYA SKLALSLWT + Sbjct: 122 LKELLLKGQNKTIINTSSNSLLFLKQFDLNTIINPKTPFKKLIGPYANSKLALSLWTNGI 181 Query: 184 APQLAAAGIMIRSADPGANNTLRSGKDSGLPFWLKPVMKLFFPHPSHGAGLLYNAALGRH 243 AP+L++ GI IRS PG N T R K G+P ++ + L F PS GA LY+AA + Sbjct: 182 APELSSEGIKIRSVCPGPNKT-RLTKGKGMPSYILLIRNLMFTPPSKGASRLYDAAF--N 238 Query: 244 SKLPGVFLIKDQITELKFAEYSSRVLSKVQAIYEQEF 280 +++ G+F+ K + T +K + + VL+++ IY+ EF Sbjct: 239 NEITGIFINKGKETAIKLSGHEEEVLNRLYDIYKNEF 275 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.135 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,562,095,601 Number of extensions: 58839501 Number of successful extensions: 210764 Number of sequences better than 1.0e-05: 1028 Number of HSP's gapped: 214008 Number of HSP's successfully gapped: 1029 Length of query: 287 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 153 Effective length of database: 1,798,280,193 Effective search space: 275136869529 Effective search space used: 275136869529 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)