BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0565.0015.p (365 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value P45511.1 GLDA_CITFR Glycerol dehydrogenase EC=1.1.1.6 353 1e-95 C1MBM3.1 C1MBM3_9ENTR Glycerol dehydrogenase 353 1e-95 A6LX24.1 A6LX24_CLOB8 Iron-containing alcohol dehydrogenase 353 2e-95 C2BAW5.1 C2BAW5_9ENTR Putative uncharacterized protein 353 2e-95 A8GJS7.1 A8GJS7_SERP5 Iron-containing alcohol dehydrogenase 352 3e-95 >P45511.1 GLDA_CITFR Glycerol dehydrogenase EC=1.1.1.6 Length = 365 Score = 353 bits (907), Expect = 1e-95, Method: Compositional matrix adjust. Identities = 181/363 (49%), Positives = 237/363 (65%), Gaps = 2/363 (0%) Query: 1 MTQIIGAPFKYIQGSGEVSRLGLYCSQLGAKGVYAVVDPFVRSLYGEEITRSFEQHSVAL 60 M ++I +P KY+QG + G Y L A + + D FV L GE++ H ++ Sbjct: 1 MLKVIQSPAKYLQGPDASTLFGQYAKNL-ADSFFVIADDFVMKLAGEKVLNGLHSHDISC 59 Query: 61 VQEEFRGECSIEQVERXXXXXXXXXXXXXXXXXXXXKTLDTAKAVSHFAGLPVVLVPTVA 120 E F GECS ++ R KTLDTAKA+ ++ LPVV++PT+A Sbjct: 60 HAERFNGECSHIEINRLIAILKQHGCRGVVGIGGG-KTLDTAKAIGYYQKLPVVVIPTIA 118 Query: 121 STDAPCSALSVLYTEDGQLDCYLPLRRNPDMVIADVDIIAKAPPRLLAAGMGDALSTYYE 180 STDAP SALSV+YTE G+ + YL +NPDMV+ D IIAKAP RLL AGMGDALST++E Sbjct: 119 STDAPTSALSVIYTEAGEFEEYLIYPKNPDMVVMDTAIIAKAPVRLLVAGMGDALSTWFE 178 Query: 181 ARACRSAGAVTQAGGSSSIAAFALARACHDTLIAYGAEALRDCREGIGSEAVGRIVEANI 240 A+AC A A + AGG S++AA +LAR C+DTL+A G +A + G+ ++A+ RIVEAN Sbjct: 179 AKACYDARATSMAGGQSTVAALSLARLCYDTLLAEGEKARFAAQAGVVTDALERIVEANT 238 Query: 241 YLSGIGFESGGLAAAHAIHNGLTLLEECRHMLHGEKVAFATLAQLMLEQAPAEEIAEATA 300 YLSGIGFES GLA AHAIHNG T+LEEC H+ HGEKVAF TLAQL+L+ +P EEI Sbjct: 239 YLSGIGFESSGLAGAHAIHNGFTILEECHHLYHGEKVAFGTLAQLVLQNSPMEEIETVLN 298 Query: 301 FCRMAGLPVTLGELGLSAAKTDRLMIAAEASCAADNPMGNMPFAVSPQQVLDAMLAADRQ 360 FC+ GLPVTL E+G+ ++M A+A+CA + NMPF+V+P+ V A+L AD Sbjct: 299 FCQKVGLPVTLAEMGVKDDIDGKIMAVAKATCAEGETIHNMPFSVTPESVHAAILTADLL 358 Query: 361 GQQ 363 GQQ Sbjct: 359 GQQ 361 >C1MBM3.1 C1MBM3_9ENTR Glycerol dehydrogenase Length = 365 Score = 353 bits (907), Expect = 1e-95, Method: Compositional matrix adjust. Identities = 181/363 (49%), Positives = 236/363 (65%), Gaps = 2/363 (0%) Query: 1 MTQIIGAPFKYIQGSGEVSRLGLYCSQLGAKGVYAVVDPFVRSLYGEEITRSFEQHSVAL 60 M ++I +P KY+QG + G Y L A + + D FV L GE++ H ++ Sbjct: 1 MLKVIQSPAKYLQGPDASTLFGQYAKNL-ADSFFVIADDFVMKLAGEKVLNGLHSHDISC 59 Query: 61 VQEEFRGECSIEQVERXXXXXXXXXXXXXXXXXXXXKTLDTAKAVSHFAGLPVVLVPTVA 120 E F GECS ++ R KTLDTAKA+ ++ LPVV++PT+A Sbjct: 60 HAERFNGECSHVEINRLIAILKQHGCRGVVGIGGG-KTLDTAKAIGYYQKLPVVVIPTIA 118 Query: 121 STDAPCSALSVLYTEDGQLDCYLPLRRNPDMVIADVDIIAKAPPRLLAAGMGDALSTYYE 180 STDAP SALSV+YTE G+ + YL +NPDMV+ D IIAKAP RLL AGMGDALST++E Sbjct: 119 STDAPTSALSVIYTEAGEFEEYLIYPKNPDMVVMDTAIIAKAPVRLLVAGMGDALSTWFE 178 Query: 181 ARACRSAGAVTQAGGSSSIAAFALARACHDTLIAYGAEALRDCREGIGSEAVGRIVEANI 240 A+AC A A + AGG S++AA +LAR C+DTL+A G +A + G+ ++A+ RIVEAN Sbjct: 179 AKACYDARATSMAGGQSTVAALSLARLCYDTLLAEGEKARFAAQAGVVTDALERIVEANT 238 Query: 241 YLSGIGFESGGLAAAHAIHNGLTLLEECRHMLHGEKVAFATLAQLMLEQAPAEEIAEATA 300 YLSGIGFES GLA AHAIHNG T+LEEC H+ HGEKVAF TLAQL+L+ +P EEI Sbjct: 239 YLSGIGFESSGLAGAHAIHNGFTILEECHHLYHGEKVAFGTLAQLVLQNSPMEEIETVLN 298 Query: 301 FCRMAGLPVTLGELGLSAAKTDRLMIAAEASCAADNPMGNMPFAVSPQQVLDAMLAADRQ 360 FC+ GLPVTL E+G+ ++M A+A+CA + NMPF V+P+ V A+L AD Sbjct: 299 FCQKVGLPVTLAEMGVKEDVDGKIMAVAKATCAEGETIHNMPFPVTPESVHAAILTADLL 358 Query: 361 GQQ 363 GQQ Sbjct: 359 GQQ 361 >A6LX24.1 A6LX24_CLOB8 Iron-containing alcohol dehydrogenase Length = 363 Score = 353 bits (906), Expect = 2e-95, Method: Compositional matrix adjust. Identities = 181/363 (49%), Positives = 245/363 (67%), Gaps = 2/363 (0%) Query: 1 MTQIIGAPFKYIQGSGEVSRLGLYCSQLGAKGVYAVVDPFVRSLYGEEITRSFEQHSVAL 60 M +I+ AP KYIQG+GE+ + Y S LG K + + + ++ + + I +SFE ++ + Sbjct: 1 MAKIMIAPSKYIQGNGELKNIAEYVSVLGEKALCLISESGLKRV-KDTIDKSFETKNIGV 59 Query: 61 VQEEFRGECSIEQVERXXXXXXXXXXXXXXXXXXXXKTLDTAKAVSHFAGLPVVLVPTVA 120 + F GECSI +V R DT KAV ++A LPVV+VPT+A Sbjct: 60 KYDLFNGECSITEVNRIIKICDENQLTVLIGIGGGKII-DTIKAVGYYANLPVVIVPTIA 118 Query: 121 STDAPCSALSVLYTEDGQLDCYLPLRRNPDMVIADVDIIAKAPPRLLAAGMGDALSTYYE 180 +TDAPCSALSVLYT+DG D YL L++NP++V+ D IIAKAP RLL +GMGDAL+TY+E Sbjct: 119 ATDAPCSALSVLYTDDGVFDKYLFLKQNPNIVLVDTGIIAKAPVRLLVSGMGDALATYFE 178 Query: 181 ARACRSAGAVTQAGGSSSIAAFALARACHDTLIAYGAEALRDCREGIGSEAVGRIVEANI 240 ARAC + A T A G+++I A ALAR C+DTLI G +A EG+ +++V +IVEAN Sbjct: 179 ARACEKSNAGTCAFGTTTITAQALARLCYDTLITEGYKAKLAVEEGVCTKSVEKIVEANT 238 Query: 241 YLSGIGFESGGLAAAHAIHNGLTLLEECRHMLHGEKVAFATLAQLMLEQAPAEEIAEATA 300 LSG+GFESGG+AAAHAIHNGLT+L C HM HGEKVAF TLAQL+LE AP +EI E Sbjct: 239 LLSGLGFESGGIAAAHAIHNGLTVLPACHHMYHGEKVAFGTLAQLVLENAPMDEIEEVLD 298 Query: 301 FCRMAGLPVTLGELGLSAAKTDRLMIAAEASCAADNPMGNMPFAVSPQQVLDAMLAADRQ 360 FC GLPVTL +LG++ K + ++ A+A+ + ++ NMPF V+P+ V A+L A+R Sbjct: 299 FCTRVGLPVTLKQLGINEIKPEEIIEVAKAATSKEDTAHNMPFEVTPEDVYAAILTANRL 358 Query: 361 GQQ 363 GQ+ Sbjct: 359 GQE 361 >C2BAW5.1 C2BAW5_9ENTR Putative uncharacterized protein Length = 368 Score = 353 bits (905), Expect = 2e-95, Method: Compositional matrix adjust. Identities = 181/363 (49%), Positives = 236/363 (65%), Gaps = 2/363 (0%) Query: 1 MTQIIGAPFKYIQGSGEVSRLGLYCSQLGAKGVYAVVDPFVRSLYGEEITRSFEQHSVAL 60 M ++I +P KY+QG + G Y L A + + D FV L GE++ H ++ Sbjct: 4 MLKVIQSPAKYLQGPDASTLFGQYAKNL-ADSFFVIADDFVMKLAGEKVLNGLHSHDISC 62 Query: 61 VQEEFRGECSIEQVERXXXXXXXXXXXXXXXXXXXXKTLDTAKAVSHFAGLPVVLVPTVA 120 E F GECS ++ R KTLDTAKA+ ++ LPVV++PT+A Sbjct: 63 HAERFNGECSHVEINRLIAILKQHGCRGVVGIGGG-KTLDTAKAIGYYQKLPVVVIPTIA 121 Query: 121 STDAPCSALSVLYTEDGQLDCYLPLRRNPDMVIADVDIIAKAPPRLLAAGMGDALSTYYE 180 STDAP SALSV+YTE G+ + YL +NPDMV+ D IIAKAP RLL AGMGDALST++E Sbjct: 122 STDAPTSALSVIYTEAGEFEEYLIYPKNPDMVVMDTAIIAKAPVRLLVAGMGDALSTWFE 181 Query: 181 ARACRSAGAVTQAGGSSSIAAFALARACHDTLIAYGAEALRDCREGIGSEAVGRIVEANI 240 A+AC A A + AGG S++AA +LAR C+DTL+A G +A + G+ ++A+ RIVEAN Sbjct: 182 AKACYDARATSMAGGQSTVAALSLARLCYDTLLAEGEKARFAAQAGVVTDALERIVEANT 241 Query: 241 YLSGIGFESGGLAAAHAIHNGLTLLEECRHMLHGEKVAFATLAQLMLEQAPAEEIAEATA 300 YLSGIGFES GLA AHAIHNG T+LEEC H+ HGEKVAF TLAQL+L+ +P EEI Sbjct: 242 YLSGIGFESSGLAGAHAIHNGFTILEECHHLYHGEKVAFGTLAQLVLQNSPMEEIETVLN 301 Query: 301 FCRMAGLPVTLGELGLSAAKTDRLMIAAEASCAADNPMGNMPFAVSPQQVLDAMLAADRQ 360 FC+ GLPVTL E+G+ ++M A+A+CA + NMPF V+P+ V A+L AD Sbjct: 302 FCQKVGLPVTLAEMGVKDDIDGKIMAVAKATCAEGETIHNMPFPVTPESVHAAILTADLL 361 Query: 361 GQQ 363 GQQ Sbjct: 362 GQQ 364 >A8GJS7.1 A8GJS7_SERP5 Iron-containing alcohol dehydrogenase Length = 368 Score = 352 bits (904), Expect = 3e-95, Method: Compositional matrix adjust. Identities = 186/362 (51%), Positives = 237/362 (65%), Gaps = 2/362 (0%) Query: 1 MTQIIGAPFKYIQGSGEVSRLGLYCSQLGAKGVYAVVDPFVRSLYGEEITRSFEQHSVAL 60 M +II +P KYIQG+ ++ +G Y L A + + D FV L G+ + S QH V Sbjct: 1 MLRIIQSPGKYIQGANALAAVGEYAKAL-ADHYFVIADDFVMKLTGDTLMGSLHQHGVKH 59 Query: 61 VQEEFRGECSIEQVERXXXXXXXXXXXXXXXXXXXXKTLDTAKAVSHFAGLPVVLVPTVA 120 F GEC ++++R KTLDTAKA++H+ LPVVL+PT+A Sbjct: 60 HACVFNGECCHKEIDRLGQELKAHGCRGVIGVGGG-KTLDTAKAIAHYQHLPVVLIPTIA 118 Query: 121 STDAPCSALSVLYTEDGQLDCYLPLRRNPDMVIADVDIIAKAPPRLLAAGMGDALSTYYE 180 STDAP SALSV+YTE G+ D YL NPDMVI D IIAKAP RLL AGMGDALSTY+E Sbjct: 119 STDAPTSALSVIYTEQGEFDEYLIHTSNPDMVIMDSSIIAKAPVRLLVAGMGDALSTYFE 178 Query: 181 ARACRSAGAVTQAGGSSSIAAFALARACHDTLIAYGAEALRDCREGIGSEAVGRIVEANI 240 A+AC A A + AGG S++AA +LAR C++TL+A G +A G+ +EAV RI+EAN Sbjct: 179 AQACFDAQATSMAGGKSTLAALSLARLCYETLLAEGVKAKLAVEAGVVTEAVERIIEANT 238 Query: 241 YLSGIGFESGGLAAAHAIHNGLTLLEECRHMLHGEKVAFATLAQLMLEQAPAEEIAEATA 300 YLSGIGFES GLAAAHAIHNG T+LEEC H+ HGEKVAF TL+QL+L+ + +I Sbjct: 239 YLSGIGFESSGLAAAHAIHNGFTVLEECHHLYHGEKVAFGTLSQLVLQNSSMAQIETVLD 298 Query: 301 FCRMAGLPVTLGELGLSAAKTDRLMIAAEASCAADNPMGNMPFAVSPQQVLDAMLAADRQ 360 FC+ GLP+TL ++G+S +++M AEASCAA NMPF V+P V A+L ADR Sbjct: 299 FCQRLGLPITLAQMGVSGDPAEKIMAVAEASCAAGETTHNMPFEVTPASVQAAILTADRL 358 Query: 361 GQ 362 G Sbjct: 359 GN 360 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.133 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,873,229,225 Number of extensions: 70126483 Number of successful extensions: 224197 Number of sequences better than 1.0e-05: 724 Number of HSP's gapped: 223006 Number of HSP's successfully gapped: 725 Length of query: 365 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 228 Effective length of database: 1,770,017,148 Effective search space: 403563909744 Effective search space used: 403563909744 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)