BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0564.0057.p (488 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KH48.1 B3KH48_9BACI Sensor protein EC=2.7.13.3 368 e-100 B1D5N0.1 B1D5N0_9BACL Sensor protein EC=2.7.13.3 350 1e-94 A7Z7J6.1 A7Z7J6_BACA2 Sensor protein EC=2.7.13.3 165 1e-38 C5ELW7.1 C5ELW7_9FIRM Putative uncharacterized protein 158 1e-36 P23545.1 PHOR_BACSU Alkaline phosphatase synthesis sensor protei... 154 2e-35 >B3KH48.1 B3KH48_9BACI Sensor protein EC=2.7.13.3 Length = 510 Score = 368 bits (944), Expect = e-100, Method: Compositional matrix adjust. Identities = 201/461 (43%), Positives = 273/461 (59%), Gaps = 8/461 (1%) Query: 31 GVGLNQQAQIEGLLAQHVKTVNLRVKQTYYTGTRLTPQAFMQQRGKALAAELAGFTGLQV 90 G+ +Q+ ++E AQ + NLR+ Q T + P FM+ G+ LA +L +G+ V Sbjct: 36 GIREDQRVRLEQTFAQEAEAANLRIHQELLTNPQTQPDDFMENVGQRLAVDLGTQSGMPV 95 Query: 91 TLYDTKGQQVGTSLQREGPAAVGNPADALAYALQNKIAYQSSGDTLLYLAPLQGPEQQMG 150 TLY G GTSL + V DALA+ Q + AY + GD LLYLAPL+ EQ +G Sbjct: 96 TLYKIDGTFTGTSLPFQPKMDV---QDALAHTAQGRSAYITEGDQLLYLAPLRNMEQPIG 152 Query: 151 VVQLEYPLQDARSFQRTLLNLFLTTGAAVLVLSFIGGYLYFNXXXXXXXXXXXXXESIRR 210 +Q L + +F + LF+ TG AVL F+ G+LY + I Sbjct: 153 TIQFHASLAEQHAFYDRIQTLFIVTGLAVLAAGFLIGFLYVWRQVHVISRLNQAAQRIGE 212 Query: 211 AEYISAPPVKRKDELGELAEGIYFMSREIDSSIAAKDEEXXXXXXXXXXXXXXXXXXXXY 270 Y+ P VKR DELGELA+GIY MS I S+ EE + Sbjct: 213 GVYLREPTVKRNDELGELAQGIYDMSGRISLSVNQLTEEKQKLLDAIDRLRELEQQQKQF 272 Query: 271 IGNISHEFKTPLTSIKAYVDLLNMYDDDPKLLSDAKLSIAKETQRLYEMVEKVLQLTALE 330 IGNISHE KTPLTSI AY DLL MY DDP LL +A+ I++E QRLY +VEK LQL++++ Sbjct: 273 IGNISHELKTPLTSILAYADLLEMYRDDPALLEEARGQISQEAQRLYRLVEKALQLSSMD 332 Query: 331 KYDFESQAELLEVADVLQDICSRMKGKAERYGLTITLDAEPAHIWIDKESFMHIFINLLD 390 Y+FE+ AE + + +LQ+ +R++ KAE+ G+T+ +W D E+ MH+ +NLLD Sbjct: 333 IYEFETHAEHVNLMPILQEAAARLEAKAEQSGITLRSSLSEGKVWADPENLMHMILNLLD 392 Query: 391 NAIKYNVPQGTVHLHGRV-RD----HRVWITVRDSGIGIPSESRDKIFEPFYTVNRDRSR 445 NAIKYN P GTV L V RD R+ I V D+GIGIP E++++IF+PFYTV+ DRSR Sbjct: 393 NAIKYNRPGGTVTLSNEVDRDPQGHDRMVIKVSDTGIGIPDEAQERIFDPFYTVSSDRSR 452 Query: 446 QSGGTGLGLSLVRNLVEKQNGTIELLEPDGEGTAFQLSFPV 486 GGTGLGLSLVRNL EKQ+G++E+ E G+ F ++ P+ Sbjct: 453 VHGGTGLGLSLVRNLAEKQHGSVEVAESSPNGSTFVVTLPM 493 >B1D5N0.1 B1D5N0_9BACL Sensor protein EC=2.7.13.3 Length = 508 Score = 350 bits (899), Expect = 1e-94, Method: Compositional matrix adjust. Identities = 200/460 (43%), Positives = 284/460 (61%), Gaps = 8/460 (1%) Query: 31 GVGLNQQAQIEGLLAQHVKTVNLRVKQTYYTGTRLTPQAFMQQRGKALAAELAGFTGLQV 90 G+ +QQ ++E +Q + NLRV++ Y TG R++P FM++ G++LA +L G +G+ V Sbjct: 41 GIREDQQRRMEQSFSQLASSANLRVREEYLTGERVSPDEFMERNGQSLAVDLGGQSGMAV 100 Query: 91 TLYDTKGQQVGTSLQREGPAAVGNPADALAYALQNKIAYQSSGDTLLYLAPLQGPEQQMG 150 TLY GQ GTSL + + + DALA+ + + AY + G+ +LYLAPL +Q +G Sbjct: 101 TLYSADGQFAGTSLPVQ---PLTDVKDALAHTAKGQEAYITLGNQVLYLAPLYNADQFLG 157 Query: 151 VVQLEYPLQDARSFQRTLLNLFLTTGAAVLVLSFIGGYLYFNXXXXXXXXXXXXXESIRR 210 VQ LQD + F R + +LF+ TGAAVL + GYLY + I Sbjct: 158 SVQFHGSLQDQKLFYRHIRDLFIGTGAAVLAAGLLLGYLYVWRQAFILGKLNQAAKDIGE 217 Query: 211 AEYISAPPVKRKDELGELAEGIYFMSREIDSSIAAKDEEXXXXXXXXXXXXXXXXXXXXY 270 Y+ PPV+R+DE+GELAEGIY MS I + + +EE + Sbjct: 218 GRYLDKPPVRRRDEIGELAEGIYDMSGSIATYVDELNEEKQKLLTAIKRLQELEQQQKQF 277 Query: 271 IGNISHEFKTPLTSIKAYVDLLNMYDDDPKLLSDAKLSIAKETQRLYEMVEKVLQLTALE 330 IGNISHE KTPLTSI AY DLL MY DDP LL +A+ I+KE +RLY ++EK LQL++++ Sbjct: 278 IGNISHELKTPLTSILAYSDLLGMYSDDPVLLQNARTQISKEAERLYALIEKALQLSSMD 337 Query: 331 KYDFESQAELLEVADVLQDICSRMKGKAERYGLTITLDAEPAHIWIDKESFMHIFINLLD 390 YDFE+ A +V +L+D SR+ KAE+ ++++ + + +W D+++ MH+ NLL Sbjct: 338 IYDFETNASRFQVKPLLEDALSRLHIKAEKQKISLSSELKDDLVWADQDNLMHMVTNLLS 397 Query: 391 NAIKYNVPQGTVHLHGRVR-----DHRVWITVRDSGIGIPSESRDKIFEPFYTVNRDRSR 445 NAIKYN P G V L R + RV I V DSGIGIP E + +IF+PF+TV+ DRSR Sbjct: 398 NAIKYNRPGGWVRLTNRSETETSGERRVIIEVADSGIGIPVELQRRIFDPFFTVSDDRSR 457 Query: 446 QSGGTGLGLSLVRNLVEKQNGTIELLEPDGEGTAFQLSFP 485 ++GGTGLGL+LVR+L EKQ+GT++L E G+ F +S P Sbjct: 458 ETGGTGLGLALVRSLAEKQHGTVQLAESGPNGSRFVISLP 497 >A7Z7J6.1 A7Z7J6_BACA2 Sensor protein EC=2.7.13.3 Length = 579 Score = 165 bits (417), Expect = 1e-38, Method: Compositional matrix adjust. Identities = 88/218 (40%), Positives = 135/218 (61%), Gaps = 3/218 (1%) Query: 270 YIGNISHEFKTPLTSIKAYVD-LLNMYDDDPKLLSDAKLSIAKETQRLYEMVEKVLQLTA 328 ++ N+SHE KTP+TSIK + + LL+ D K LSD I KE++RL +V+ +L L+ Sbjct: 356 FVANVSHELKTPITSIKGFTETLLDGAMKDEKALSDFLSIILKESERLQSLVQDLLDLSK 415 Query: 329 LEKYDFESQAELLEVADVLQDICSRMKGKAERYGLTITLD--AEPAHIWIDKESFMHIFI 386 +E+ +F + E + A +L +I + ++ KAE G++ LD EP + D +F+ Sbjct: 416 MEQQNFTMRVESFDAAKILHEIEALLRHKAEEKGISFQLDLPEEPIFVTGDAHRLKQVFL 475 Query: 387 NLLDNAIKYNVPQGTVHLHGRVRDHRVWITVRDSGIGIPSESRDKIFEPFYTVNRDRSRQ 446 NL +NA+ Y +GTV + V++ V I V D+GIGI E +IFE FY V++DRSR Sbjct: 476 NLANNALTYTPEKGTVGISVHVKETVVDIKVSDTGIGIQKEEIPRIFERFYRVDKDRSRN 535 Query: 447 SGGTGLGLSLVRNLVEKQNGTIELLEPDGEGTAFQLSF 484 SGGTGLGL++V++L+E G IE+ G+GT F ++ Sbjct: 536 SGGTGLGLAIVKHLIEAHEGKIEVTSEPGQGTVFTVTL 573 >C5ELW7.1 C5ELW7_9FIRM Putative uncharacterized protein Length = 468 Score = 158 bits (399), Expect = 1e-36, Method: Compositional matrix adjust. Identities = 119/437 (27%), Positives = 203/437 (46%), Gaps = 39/437 (8%) Query: 58 TYYTGTRLTPQAFMQQ---RGKALAAELAGFTGLQVTLYDTKG-QQVGTSLQREGPAAVG 113 T Y G R+ + Q K L AE+ + D++G +V T + PAA+G Sbjct: 57 TMYQGKRMDSEEVTSQIRALSKYLRAEIWVVNRQGTIVMDSQGGSRVQTDISNFDPAAMG 116 Query: 114 NPADALA--YALQNKIAYQSSGDTLLYLAPLQGPEQQMGVVQLEYPLQDARSFQRTLLNL 171 N + Y + N+ + L AP+ G G V + PL Q +LN+ Sbjct: 117 NRSYTTGRFYGMFNE-------NVLSVSAPITGNYNTYGYVLIHLPLSQITQSQNDILNI 169 Query: 172 FLTTGAAVLVLSFIGGYLYFNXXXXXXXXXXXXXESIRRAEYISAPPVKRKDELGELAEG 231 T A V LS I ++ VK DE+G LA+ Sbjct: 170 LYITSAIVFALSLIILLVFTQTVYLPLRKITVGANEYAAGNLEYRINVKTHDEVGYLADT 229 Query: 232 IYFMSREIDSSIAAKDEEXXXXXXXXXXXXXXXXXXXXYIGNISHEFKTPLTSIKAYVDL 291 + +MS E++ K EE +I N+SH+F++PLTSIK Y++ Sbjct: 230 LNYMSDELN-----KMEEYQKN----------------FIANVSHDFRSPLTSIKGYLEA 268 Query: 292 LNMYDDDPKLLSDAKLS-IAKETQRLYEMVEKVLQLTALEKYDFESQAELLEVADVLQDI 350 + + P+ + + LS + ET+RL+++ E +L L +L+ + S+ ++ V++D Sbjct: 269 I-LDGTIPEDMYEKYLSRVISETKRLHKLTESMLTLNSLDAKGYLSRTNF-DINRVIKDT 326 Query: 351 CSRMKGK--AERYGLTITLDAEPAHIWIDKESFMHIFINLLDNAIKYNVPQGTVHLHGRV 408 + +G A+ +T ++ D + NL+DNAIK++ T+++ Sbjct: 327 AASFEGTCDAKNISFDLTFSDSIQMVYADLGKIQQVLYNLIDNAIKFSHHNSTIYIQASG 386 Query: 409 RDHRVWITVRDSGIGIPSESRDKIFEPFYTVNRDRSRQSGGTGLGLSLVRNLVEKQNGTI 468 R +++++V+D+GIGIP +S+ KI+E FY + R + GTGLGLS+V+ +V+ I Sbjct: 387 RYEKIFVSVKDTGIGIPKDSQKKIWERFYKTDLSRGKDKKGTGLGLSIVKEIVQSHGENI 446 Query: 469 ELLEPDGEGTAFQLSFP 485 +++ +G GT F S P Sbjct: 447 DVVSTEGVGTEFIFSLP 463 >P23545.1 PHOR_BACSU Alkaline phosphatase synthesis sensor protein phoR EC=2.7.13.3 Length = 579 Score = 154 bits (389), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 83/218 (38%), Positives = 137/218 (62%), Gaps = 3/218 (1%) Query: 270 YIGNISHEFKTPLTSIKAYVD-LLNMYDDDPKLLSDAKLSIAKETQRLYEMVEKVLQLTA 328 ++ N+SHE KTP+TSIK + + LL+ +D + LS+ I KE++RL +V+ +L L+ Sbjct: 354 FVANVSHELKTPITSIKGFTETLLDGAMEDKEALSEFLSIILKESERLQSLVQDLLDLSK 413 Query: 329 LEKYDFESQAELLEVADVLQDICSRMKGKAERYGLTITLDA--EPAHIWIDKESFMHIFI 386 +E+ +F E E A +L +I + +K KA+ G+++ L+ +P ++ D +F+ Sbjct: 414 IEQQNFTLSIETFEPAKMLGEIETLLKHKADEKGISLHLNVPKDPQYVSGDPYRLKQVFL 473 Query: 387 NLLDNAIKYNVPQGTVHLHGRVRDHRVWITVRDSGIGIPSESRDKIFEPFYTVNRDRSRQ 446 NL++NA+ Y G+V ++ + R+ + I V DSGIGI E +IFE FY V++DRSR Sbjct: 474 NLVNNALTYTPEGGSVAINVKPREKDIQIEVADSGIGIQKEEIPRIFERFYRVDKDRSRN 533 Query: 447 SGGTGLGLSLVRNLVEKQNGTIELLEPDGEGTAFQLSF 484 SGGTGLGL++V++L+E G I++ G GT F ++ Sbjct: 534 SGGTGLGLAIVKHLIEAHEGKIDVTSELGRGTVFTVTL 571 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.137 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,547,725,700 Number of extensions: 98925057 Number of successful extensions: 371890 Number of sequences better than 1.0e-05: 31239 Number of HSP's gapped: 327735 Number of HSP's successfully gapped: 31346 Length of query: 488 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 348 Effective length of database: 1,741,754,103 Effective search space: 606130427844 Effective search space used: 606130427844 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)