BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0564.0040.p (328 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q826F4.1 Q826F4_STRAW Putative heat shock protein DnaJ 154 9e-36 B4UXD9.1 B4UXD9_9ACTO Heat shock protein DnaJ 149 3e-34 C1R536.1 C1R536_9ACTO DnaJ-class molecular chaperone with C-term... 125 7e-27 Q2JB01.1 Q2JB01_FRASC Heat shock protein DnaJ-like 117 2e-24 C4EGK6.1 C4EGK6_STRRS DnaJ-class molecular chaperone with C-term... 108 8e-22 >Q826F4.1 Q826F4_STRAW Putative heat shock protein DnaJ Length = 317 Score = 154 bits (390), Expect = 9e-36, Method: Compositional matrix adjust. Identities = 106/331 (32%), Positives = 152/331 (45%), Gaps = 32/331 (9%) Query: 1 LAANYYEALGVGEKASKQEIKKAYQKLAKKWHPDVNXXXXXXXXXXXXXXXXXVLGNDEK 60 +A +YYE LGV AS+ EI++AY+KLA++ HPDVN VL + + Sbjct: 1 MARDYYEVLGVSRSASQDEIQQAYRKLARRHHPDVNKDPGAEERFKDLNEAYSVLSDPKT 60 Query: 61 RKAYDEELXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIPDD-------- 112 R YD DD Sbjct: 61 RARYDRFGEDFRKIPEDFDERVAAGAGGGFRGRRTAGGGGPRVRYAPGFGDDFGAEGIDI 120 Query: 113 -DLFGMFFGSRGAADRAXXXXXXXXXXXXXEARSTMQAQLEVSLEQAYKGGSIRVQVGGK 171 DLFG FG+ GAA + +A+L +++E+AY+GG V + G Sbjct: 121 EDLFGSMFGA-GAARGGVPG-------------ADQEAELPLTVEEAYRGGRRTVTLAGP 166 Query: 172 D-----VNVSIPPRSAEGTVIRIPGSSGNGAGQD--GDLLISLHLLPHNIYEPDGGDLYG 224 D V +PP +G IR+ G G G+G GDL + + + PH + DG D++ Sbjct: 167 DGQPRRYEVDVPPGVTDGQRIRLAGEGGRGSGDAAAGDLYLRVRIQPHPRFRLDGRDVHV 226 Query: 225 TVEIAPWQAVLGGEAKVPLPDGSSVKLKVPAGMAGGQTLRLSGKGLKRPNGANGDILFAV 284 V +APW+A LG VP P G + K+ VPAG + G+ LRL G+G+ P GANGD+ + Sbjct: 227 QVPVAPWEAALGATVPVPTPGGGTAKVTVPAGSSSGRRLRLRGEGMPNPRGANGDLYAEL 286 Query: 285 EIVIPAGLTEAEKKLYRQLAEAGSFQAGPRR 315 IV+P L + E++L+ +LA SF PRR Sbjct: 287 RIVVPPTLGDQERELFEELAATSSFD--PRR 315 >B4UXD9.1 B4UXD9_9ACTO Heat shock protein DnaJ Length = 315 Score = 149 bits (376), Expect = 3e-34, Method: Compositional matrix adjust. Identities = 105/331 (31%), Positives = 155/331 (46%), Gaps = 33/331 (9%) Query: 1 LAANYYEALGVGEKASKQEIKKAYQKLAKKWHPDVNXXXXXXXXXXXXXXXXXVLGNDEK 60 +A +YYEALGV AS+ EI++AY+KLA++ HPDVN VL +D K Sbjct: 1 MARDYYEALGVSRSASQDEIQQAYRKLARRHHPDVNKDPGAEERFKDLNEAYGVL-SDPK 59 Query: 61 RKAYDEELXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIPDD-------- 112 +A + DD Sbjct: 60 NRARYDRFGEDFRQIPEDFDERVAAGAGDGFRGRTTAGDGGPRVRYTGFGDDFGAEGIDI 119 Query: 113 -DLFGMFFGSRGAADRAXXXXXXXXXXXXXEARSTMQAQLEVSLEQAYKGGSIRVQVGG- 170 DLFG FG+ GAA + +A+L +++E+AY+GG V + G Sbjct: 120 EDLFGSMFGA-GAARGGVPG-------------ADQEAELALTVEEAYRGGRRTVTLAGP 165 Query: 171 ----KDVNVSIPPRSAEGTVIRIPGSSGNGAGQD--GDLLISLHLLPHNIYEPDGGDLYG 224 + V +PP +G IR+ G G G+G GDL + + + PH + DG D++ Sbjct: 166 TGQPRRYKVDVPPGVTDGQRIRLAGEGGRGSGDAAAGDLYLRVRIQPHPRFRLDGRDVHV 225 Query: 225 TVEIAPWQAVLGGEAKVPLPDGSSVKLKVPAGMAGGQTLRLSGKGLKRPNGANGDILFAV 284 V +APW+A LG VP P G + K+ VPAG + G+ LRL G+G+ P GA+GD+ + Sbjct: 226 QVPVAPWEAALGATVPVPTPGGGTAKVTVPAGSSSGRRLRLRGEGMPNPRGADGDLYAEL 285 Query: 285 EIVIPAGLTEAEKKLYRQLAEAGSFQAGPRR 315 IV+P L + E++L+ +LA A S+ PRR Sbjct: 286 RIVVPPTLGDRERELFEELAAASSYD--PRR 314 >C1R536.1 C1R536_9ACTO DnaJ-class molecular chaperone with C-terminal Zn finger domain Length = 324 Score = 125 bits (313), Expect = 7e-27, Method: Compositional matrix adjust. Identities = 69/173 (39%), Positives = 105/173 (60%), Gaps = 8/173 (4%) Query: 149 AQLEVSLEQAYKGGSIRVQVGG---KDVNVSIPPRSAEGTVIRIPGSSGNGAGQD--GDL 203 A+LE+S+E+AY+GG ++ +GG +D +V+IP +G IR+ G G G G GDL Sbjct: 154 AELELSVEEAYRGGKRKITIGGAGGRDYDVTIPAGVVDGQRIRLAGQGGRGYGDAPAGDL 213 Query: 204 LISLHLLPHNIYEPDGGDLYGTVEIAPWQAVLGGEAKVPLPDGSSVKLKVPAGMAGGQTL 263 + + + PH + G D+ + ++PW+A LG VP P G + K+ VPAG + G+ L Sbjct: 214 YLQIRIAPHPRFRLSGRDITVDLPVSPWEAALGATVPVPTP-GGTAKVVVPAGSSTGRKL 272 Query: 264 RLSGKGLKRPNGANGDILFAVEIVIPAGLTEAEKKLYRQLAEAGSFQAGPRRK 316 RL G+G+ P G GD+ V I++PA T+ E++L+ QLA A SF PRR+ Sbjct: 273 RLRGEGMPNPKGPPGDLYAEVRIMVPATPTDRERELFEQLAAASSFD--PRRR 323 >Q2JB01.1 Q2JB01_FRASC Heat shock protein DnaJ-like Length = 335 Score = 117 bits (292), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 70/204 (34%), Positives = 105/204 (51%), Gaps = 22/204 (10%) Query: 113 DLFGMFFGSRGAADRAXXXXXXXXXXXXXEARSTMQAQLEVSLEQAYKGGSIRVQVGG-- 170 DLFG FG+ GA + +A+LE+++E+AY+GG ++ + G Sbjct: 143 DLFGDLFGAHGATGPIPG--------------ADQEAELELTVEEAYRGGRRQITLAGPG 188 Query: 171 ---KDVNVSIPPRSAEGTVIRIPGSSGNGA--GQDGDLLISLHLLPHNIYEPDGGDLYGT 225 + V+IP EG +R+ G G G+ GQ GDL + + + PH Y G D+ Sbjct: 189 GSPRSYQVTIPRGVVEGQRVRLAGQGGRGSRHGQPGDLYLHVKIAPHPYYRLSGRDINVP 248 Query: 226 VEIAPWQAVLGGEAKVPLPDGSSVKLKVPAGMAGGQTLRLSGKGLKRPNGANGDILFAVE 285 + + PW+A LG V P G +K++VPAG + G+ LRL G GL P G GD+ V Sbjct: 249 LPVTPWEAALGARVPVTTP-GGEIKVRVPAGSSSGRRLRLRGAGLPNPTGPPGDLHAEVR 307 Query: 286 IVIPAGLTEAEKKLYRQLAEAGSF 309 + +P LT E+ L+ QLA +F Sbjct: 308 VAVPPALTARERALFEQLAAVSAF 331 >C4EGK6.1 C4EGK6_STRRS DnaJ-class molecular chaperone with C-terminal Zn finger domain Length = 317 Score = 108 bits (270), Expect = 8e-22, Method: Compositional matrix adjust. Identities = 65/171 (38%), Positives = 105/171 (61%), Gaps = 5/171 (2%) Query: 148 QAQLEVSLEQAYKGGSIRVQVGGKDVNVSIPPRSAEGTVIRIPGSSGNGA--GQDGDLLI 205 +A++E+++E+AY+GG + VGG+ ++V+IP +G IR+ G G G+ GDL + Sbjct: 150 EAEIELTVEEAYRGGRRSITVGGRRIDVNIPAGVTDGQRIRLAGQGGRGSDGAAAGDLYL 209 Query: 206 SLHLLPHNIYEPDGGDLYGTVEIAPWQAVLGGEAKVPLPDGSSVKLKVPAGMAGGQTLRL 265 + + PH Y +G D+ + + PW+A LG V P G + K+KVPAG + G+ LRL Sbjct: 210 IVRIAPHPRYRVEGRDIRVQLPVTPWEAALGASVAVDTPAGEA-KVKVPAGSSSGRRLRL 268 Query: 266 SGKGLKRPNGANGDILFAVEIVIPAGLTEAEKKLYRQLAEAGSFQAGPRRK 316 G+G+ P G GD+ V I++PA L++ E++L+ QLA +F PRR+ Sbjct: 269 RGRGMPNPRGTPGDLFAEVRIMVPAALSDEERRLFEQLAAVSTFD--PRRR 317 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.313 0.135 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,661,057,463 Number of extensions: 67537380 Number of successful extensions: 140742 Number of sequences better than 1.0e-05: 2914 Number of HSP's gapped: 134798 Number of HSP's successfully gapped: 3329 Length of query: 328 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 192 Effective length of database: 1,779,438,163 Effective search space: 341652127296 Effective search space used: 341652127296 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 131 (55.1 bits)