BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0564.0031.p (404 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C4CU21.1 C4CU21_9SPHI Predicted dehydrogenase 235 6e-60 C4D0A9.1 C4D0A9_9SPHI Predicted dehydrogenase 228 9e-58 C1S3S8.1 C1S3S8_9SPHI Predicted dehydrogenase 226 4e-57 C2G2U5.1 C2G2U5_9SPHI Alpha-N-acetylgalactosaminidase EC=3.2.1.49 224 1e-56 A1ZQ32.1 A1ZQ32_9SPHI Oxidoreductase, Gfo/Idh/MocA family 224 2e-56 >C4CU21.1 C4CU21_9SPHI Predicted dehydrogenase Length = 457 Score = 235 bits (600), Expect = 6e-60, Method: Compositional matrix adjust. Identities = 148/419 (35%), Positives = 217/419 (51%), Gaps = 40/419 (9%) Query: 6 IRLAIVG-GNRGSRFYDLLAWIADRIRLAAICDVD-------DRILSNWKEQYPSIQTY- 56 +RLA +G G RG + +D + + A+CD+ + ++ + P T Sbjct: 42 VRLAFIGVGLRGRNHLQQALYRSD-VEVTALCDISADAIAKSNAMIEKAGRKTPPAYTKG 100 Query: 57 -QNYEQLLQNPDIDAVFLASPMLLHARQSIDALKAGKHVLSEVIAAHTVEEAWELVETVE 115 + + +L+ DID V +A+P H ++ ++KAGK+ EV A T++E+W+LV T E Sbjct: 101 DEAFLDMLKRDDIDGVVIATPWEWHTPMAVASMKAGKYTGLEVSATVTLKESWDLVNTYE 160 Query: 116 QTGLVYMMAENYCFSRTSLMIRHMAEQGLFGEITYMEGGYIHDLRALVHQPDGS------ 169 +T M+ EN C+ R + I +M QG+FGE+TY GY HDLR + DG+ Sbjct: 161 KTKSHCMLLENVCYRRDVMAILNMVRQGMFGEMTYAHCGYEHDLRNIKFN-DGTARGVGA 219 Query: 170 ---------LNWRGQLHHTYNGMNYPTHSLGPLAGWIAVNKQGGDQLESISTYVSNSRAL 220 +WR Q NG YPTH LGP+A W+ +N+ G+Q +++ + SR L Sbjct: 220 EFGEKGFSEAHWRTQHSVDRNGDLYPTHGLGPVAHWLNINR--GNQFVRLTSMATKSRGL 277 Query: 221 RKYFHEQFGGNHPAAQDGYWKQGDCAVTAVRTRGGVLITLRVDWTSVRPHNMRHYALQGT 280 KY ++ G NHP A+ + K GD T V G I + D S RP+++ A QGT Sbjct: 278 HKYVVDKGGANHPNAKVNF-KLGDVVTTMVECANGENIVIIHDTNSPRPYSLGFRA-QGT 335 Query: 281 AGAYLSARHDQEGDLIWLRDRSPIADDGADKWESLERYRPEYDHPLWKQWXXXXXXXXXX 340 G ++ +GD I+L SP +WES Y+ +YDHPLWKQ Sbjct: 336 QGIWM-----DDGDTIYLEGVSP----KPHQWESFSPYQEKYDHPLWKQHVETAQNAGHG 386 Query: 341 XXDFLVMEEFASAITEQRSPAIDVYDAVTWSSIFPLSMESVAAGGKPLAIPDFARNRGK 399 DF V+ F +I Q +P IDVYDA WS+I PLS +S+A G K + IPDF R + K Sbjct: 387 GIDFFVLRGFIESIKNQVAPPIDVYDAAAWSAISPLSEQSIAGGSKAVDIPDFTRGKWK 445 >C4D0A9.1 C4D0A9_9SPHI Predicted dehydrogenase Length = 458 Score = 228 bits (581), Expect = 9e-58, Method: Compositional matrix adjust. Identities = 142/419 (33%), Positives = 213/419 (50%), Gaps = 39/419 (9%) Query: 6 IRLAIVG-GNRGSRFYDLLAWIADRIRLAAICDVDDRILSNW---------KEQYPSIQT 55 +RLA +G G+RG R + A D + + AICD +S K + Sbjct: 42 VRLAFIGVGSRG-RSHVEQALYRDDVEITAICDPAPEAISRTTAMIEKAGRKAPVAYSKG 100 Query: 56 YQNYEQLLQNPDIDAVFLASPMLLHARQSIDALKAGKHVLSEVIAAHTVEEAWELVETVE 115 + ++ +L+ DID V +A+P H ++ + AGK+ EV A T++E+W+LV E Sbjct: 101 DEAFKDMLKRDDIDGVVIATPWEWHVPMAVATMNAGKYAGVEVSATVTLKESWDLVNAFE 160 Query: 116 QTGLVYMMAENYCFSRTSLMIRHMAEQGLFGEITYMEGGYIHDLRALVHQPDGSL----- 170 +TG M+ EN C+ R L + +M +G+FGE+TY GY HDLR + S+ Sbjct: 161 KTGSPCMILENVCYRRDVLAVLNMIRKGMFGEMTYAHCGYQHDLRNIKFNDGKSIGGVGA 220 Query: 171 ----------NWRGQLHHTYNGMNYPTHSLGPLAGWIAVNKQGGDQLESISTYVSNSRAL 220 +WR Q NG YPTH LGP+A W+ +N+ G++ +++ + SR L Sbjct: 221 EFGEKGYSEAHWRTQHSVDRNGDLYPTHGLGPVAHWLDINR--GNRFTHLTSTATKSRGL 278 Query: 221 RKYFHEQFGGNHPAAQDGYWKQGDCAVTAVRTRGGVLITLRVDWTSVRPHNMRHYALQGT 280 KY ++ G NHP A+ + K GD T ++ G I + D S RP+++ A QGT Sbjct: 279 HKYVVDKGGPNHPNAKVNF-KLGDVVTTVIQCANGENIVIIHDTNSPRPYSLGFRA-QGT 336 Query: 281 AGAYLSARHDQEGDLIWLRDRSPIADDGADKWESLERYRPEYDHPLWKQWXXXXXXXXXX 340 G ++ + D+I+L SP WE Y+ +YDHPLWK+ Sbjct: 337 NGIWM-----DDNDMIYLEGVSP----KPHSWEPFAAYQEKYDHPLWKRHAQTAENAGHG 387 Query: 341 XXDFLVMEEFASAITEQRSPAIDVYDAVTWSSIFPLSMESVAAGGKPLAIPDFARNRGK 399 DF V+ F +I + IDVYDA WS+I PLS +S+A G KP+AIPDF + + K Sbjct: 388 GIDFFVLRAFIESIKAKGPVPIDVYDAAVWSAISPLSEQSIAGGSKPVAIPDFTKGKWK 446 >C1S3S8.1 C1S3S8_9SPHI Predicted dehydrogenase Length = 464 Score = 226 bits (575), Expect = 4e-57, Method: Compositional matrix adjust. Identities = 146/431 (33%), Positives = 215/431 (49%), Gaps = 49/431 (11%) Query: 1 MTQQPIRLAIVG-GNRGSRFYDLLAWIADRIRLAAICDVDDR--------ILSNWKEQYP 51 + ++ +R+ ++G G RG DL D + + AICD D + +++ Sbjct: 35 IKKEKVRVGLIGVGARGQGHLDLCLH-RDDVDVVAICDPDTQWAIPKSRELITKAYGAKK 93 Query: 52 SIQTYQN----YEQLLQNPDIDAVFLASPMLLHARQSIDALKAGKHVLSEVIAAHTVEEA 107 + Y N + LL+ DIDAV +A+P H Q+I A+KAGK EV A ++E Sbjct: 94 KVAEYSNGPEDFHNLLKRDDIDAVIIATPWEWHTIQAIAAMKAGKTPAVEVCGASDIQEC 153 Query: 108 WELVETVEQTGLVYMMAENYCFSRTSLMIRHMAEQGLFGEITYMEGGYIHDLRALVHQPD 167 W LV T E TG+ EN C+ R + + +M QGLFGE+T+++GGY HDLR + Sbjct: 154 WNLVNTSEDTGVPVFGMENVCYRRDVMAVLNMVRQGLFGELTHLQGGYQHDLRKVKFNNG 213 Query: 168 GSL---------------NWRGQLHHTYNGMNYPTHSLGPLAGWIAVNKQGGDQLESIST 212 L WR NG YPTH LGP+A I +N+ G++L S+++ Sbjct: 214 KQLYGGGVEFGEKAMSEAKWRTNHSVHRNGDLYPTHGLGPVANMININR--GNRLLSLTS 271 Query: 213 YVSNSRALRKYFHEQFGGNHPAAQDGYWKQGDCAVTAVRTRGGVLITLRVDWTSVRPHNM 272 + SR L KY + G NHP A+ + K GD T +RT G I L D S RP+++ Sbjct: 272 VATKSRGLHKYVVDNGGENHPNAKVEF-KLGDIVTTLLRTNNGETIMLSHDTNSPRPYSL 330 Query: 273 RHYALQGTAGAYLSARHDQEGDLIWLRDRSPIADDGADKWESL------ERYRPEYDHPL 326 ++ +QGT G ++ + D I++ +SP D+WE Y +YDHPL Sbjct: 331 -NFRVQGTNGLWM-----DDFDSIYVEGKSPY-----DEWEKAGSDKDPNSYMGKYDHPL 379 Query: 327 WKQWXXXXXXXXXXXXDFLVMEEFASAITEQRSPAIDVYDAVTWSSIFPLSMESVAAGGK 386 W+++ D+ V+ F ++ A+DVYD TW +I PLS +SVA GG Sbjct: 380 WRRYTSSAAGAGHGGMDWFVINSFIESVKRGAPYALDVYDLATWYAITPLSEQSVAEGGN 439 Query: 387 PLAIPDFARNR 397 IPDF R R Sbjct: 440 VQYIPDFTRGR 450 >C2G2U5.1 C2G2U5_9SPHI Alpha-N-acetylgalactosaminidase EC=3.2.1.49 Length = 447 Score = 224 bits (571), Expect = 1e-56, Method: Compositional matrix adjust. Identities = 139/423 (32%), Positives = 213/423 (50%), Gaps = 45/423 (10%) Query: 6 IRLAIVGGNRGSRFYDLLAWIADRIRLAAICDVDDRILSNWKEQYPS---------IQTY 56 +R+A +G R + +A D + + AICD + L+ ++Q+ + Sbjct: 32 VRIAFIGVGLRGRNHVAIALNRDDVEIVAICDTQEESLTQCRKQFDKKGAKLPKEYTGSV 91 Query: 57 QNYEQLLQNPDIDAVFLASPMLLHARQSIDALKAGKHVLSEVIAAHTVEEAWELVETVEQ 116 Y+++L N +DAV +A+P H Q+IDA+KAG +V EVIA TV++ W++V+ EQ Sbjct: 92 DAYKKMLDNEKLDAVIIATPWEFHKNQAIDAMKAGLYVGCEVIAGLTVKDHWDVVKASEQ 151 Query: 117 TGLVYMMAENYCFSRTSLMIRHMAEQGLFGEITYMEGGYIHDLRALVHQPDGSL------ 170 TG YM EN + R L + +M QGLFGEI ++EGGY HDLR ++ S Sbjct: 152 TGKPYMTLENVAYRRDVLAVLNMHRQGLFGEILHLEGGYQHDLREVLFNDGKSYYGKGVE 211 Query: 171 ---------NWRGQLHHTYNGMNYPTHSLGPLAGWIAVNKQGGDQLESISTYVSNSRALR 221 WR + + +G YPTH LGP+ + +N G++ I++Y S +R L Sbjct: 212 FGPKAISEAQWRTKYNVEQDGDLYPTHGLGPIMHFADIN--AGNRFTHITSYSSKARGLA 269 Query: 222 KYFHEQFGGNHPAAQDGYWKQGDCAVTAVRTRGGVLITLRVDWTSVRPHNMRHYALQGTA 281 Y E+ HP A+ Y K GD T ++ G + L D RP+++ + +QGT Sbjct: 270 AYV-EKMSPGHPNAKLNY-KNGDITQTMLQCANGETMLLTHDTHLPRPYSIG-FRVQGTK 326 Query: 282 GAYLSARHDQEGDLIWLRDRSPIADDGADK---WESLERYRPEYDHPLWKQWXXXXXXXX 338 G IW+ + I +G K W+ + +YDHP+WK++ Sbjct: 327 G-------------IWMDVANGIHIEGQSKPHAWDPAADWVKKYDHPIWKKYEEVANGSG 373 Query: 339 XXXXDFLVMEEFASAITEQRSPAIDVYDAVTWSSIFPLSMESVAAGGKPLAIPDFARNRG 398 D+ V F A+ ++R IDVYD+VT S++FPLS ES+ AG K L PDF + Sbjct: 374 HGGMDWFVFNGFIQAVKQKRQTPIDVYDSVTMSAVFPLSTESIKAGNKTLEFPDFTSGKW 433 Query: 399 KQE 401 K + Sbjct: 434 KTK 436 >A1ZQ32.1 A1ZQ32_9SPHI Oxidoreductase, Gfo/Idh/MocA family Length = 454 Score = 224 bits (570), Expect = 2e-56, Method: Compositional matrix adjust. Identities = 133/418 (31%), Positives = 214/418 (51%), Gaps = 37/418 (8%) Query: 4 QPIRLAIVGGNRGSRFYDLLAWIADRIRLAAICDVDDRILSNWKE------QYPSIQTYQ 57 + +++ +G R + LA + AICD+D + + +E + ++ Sbjct: 36 RKVKIGFIGVGLRGREHLRLALQRKDTEVVAICDIDPQAIKISQEMIRKAGRKKAVAFSD 95 Query: 58 N---YEQLLQNPDIDAVFLASPMLLHARQSIDALKAGKHVLSEVIAAHTVEEAWELVETV 114 N Y++LL+ DI V +A+P L H R ++D +K+GK+ EV AA+T++E W+LV T Sbjct: 96 NELSYKKLLERTDIQGVIIATPWLWHTRMAVDVMKSGKYAGVEVSAANTLQECWDLVNTS 155 Query: 115 EQTGLVYMMAENYCFSRTSLMIRHMAEQGLFGEITYMEGGYIHDLRALV----HQPDGS- 169 E TG+ M+ EN C+ R + + +M Q +FGE+ ++E GY HDLR + QP G Sbjct: 156 EATGMPCMILENVCYRRDVMAVLNMVRQNMFGEMIHLECGYQHDLRHVKFNNGKQPYGGG 215 Query: 170 ----------LNWRGQLHHTYNGMNYPTHSLGPLAGWIAVNKQGGDQLESISTYVSNSRA 219 WR NG YPTH +GP+A + +N+ G++ +++ + SR Sbjct: 216 VEFGEKGFSEAKWRTAHSVNRNGDLYPTHGIGPVANYTNINR--GNRFLYLTSTATKSRG 273 Query: 220 LRKYFHEQFGGNHPAAQDGYWKQGDCAVTAVRTRGGVLITLRVDWTSVRPHNMRHYALQG 279 L Y E+ G +HP A+ + K GD T ++T G I + D RP+++ + +QG Sbjct: 274 LHNYIVEKGGKDHPNAKVRF-KLGDIITTVIKTANGESIIVSHDTNLPRPYSL-GFRVQG 331 Query: 280 TAGAYLSARHDQEGDLIWLRDRSPIADDGADKWESLERYRPEYDHPLWKQWXXXXXXXXX 339 T G +++ + +++ SP A WES Y +YDHPLWK++ Sbjct: 332 TKGLWMAVNNS-----LFIEGTSP----RAHVWESARSYLKKYDHPLWKKYEKDATGAGH 382 Query: 340 XXXDFLVMEEFASAITEQRSPAIDVYDAVTWSSIFPLSMESVAAGGKPLAIPDFARNR 397 D+ V+ F +I + S +D YDA WS+I PLS +S+A G +P PDF R R Sbjct: 383 GGMDYFVLHAFVESIKRKTSTPLDAYDAAAWSAITPLSEQSIAQGSQPAHFPDFTRGR 440 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.135 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,541,176,556 Number of extensions: 104621779 Number of successful extensions: 276451 Number of sequences better than 1.0e-05: 1521 Number of HSP's gapped: 276416 Number of HSP's successfully gapped: 1521 Length of query: 404 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 266 Effective length of database: 1,760,596,133 Effective search space: 468318571378 Effective search space used: 468318571378 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)