BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0564.0004.p (337 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A1HM22.1 A1HM22_9FIRM Uncharacterized protein UPF0065 271 4e-71 C2BF20.1 C2BF20_9FIRM TTT family tricarboxylate transporter, rec... 259 3e-67 B7LU06.1 B7LU06_ESCF3 Putative periplasmic binding protein 248 4e-64 Q0TAM6.1 Q0TAM6_ECOL5 Putative uncharacterized protein 248 7e-64 B3HXB1.1 B3HXB1_ECOLX Periplasmic binding protein 248 7e-64 >A1HM22.1 A1HM22_9FIRM Uncharacterized protein UPF0065 Length = 327 Score = 271 bits (694), Expect = 4e-71, Method: Compositional matrix adjust. Identities = 150/304 (49%), Positives = 203/304 (66%), Gaps = 2/304 (0%) Query: 34 SHKGDNADFPGKPITLIVPYXXXXXXXXXXXXXXXXXEKVLGQPVIVVNRTGGGGSVGLM 93 + K D FP K I +IVPY + + GQPV+VVN+ GGGG+VG+ Sbjct: 25 AQKTDAPKFPTKQIEIIVPYAAGGGTDQVARALANSAKDIPGQPVVVVNKVGGGGAVGMS 84 Query: 94 EGAGAKGDGYTVTFLPAELTILPHLGLLPITYEKFKPIAQTNFDPSAITVRQGAPWQTVN 153 EG AK DGYTVT + EL LPHLGL PI Y+ F+PI + N DP+A+TVR APW+T+ Sbjct: 85 EGEKAKPDGYTVTMITVELVTLPHLGLAPINYQNFEPILRVNMDPAAVTVRADAPWKTLK 144 Query: 154 EFLGFAKAHPGELKMGNAGTGSIWHLAAITLERETGVKFAHIPFEGAGPAVSALMDGFVD 213 EFL +AKA+PG++++GN+G G+IWHLAA +LE++TGV+F H+PF GA PAV+AL+ G V+ Sbjct: 145 EFLDYAKANPGKVRVGNSGPGAIWHLAAASLEQKTGVQFTHVPFNGAAPAVTALLGGNVE 204 Query: 214 AVPVSPAEVKKYVDEGKLRTLAVNADERSDALPGVPTLEELTGIHVNFTGTWRGLAVPKD 273 AV VSPAEV V G++R L V +D+R+ A+P VPTL+E GI + GTWRGLAVPK Sbjct: 205 AVTVSPAEVSAQVAAGQVRVLGVMSDKRAKAVPDVPTLKE-QGIDL-VIGTWRGLAVPKG 262 Query: 274 TPDAIADILAGAFIKGTEDKAFREYMNVNGLGLLVKDGKAFSRQLKESDDTFAKMIPELG 333 TP + L AF K + KAF +YM+ GLG+ + + LK +D F +I +LG Sbjct: 263 TPAPVVKTLHDAFKKAMDSKAFTDYMDKAGLGIGYLSSEDYKASLKVENDNFKALIEKLG 322 Query: 334 LSRR 337 L+++ Sbjct: 323 LNKK 326 >C2BF20.1 C2BF20_9FIRM TTT family tricarboxylate transporter, receptor protein Length = 330 Score = 259 bits (662), Expect = 3e-67, Method: Compositional matrix adjust. Identities = 132/298 (44%), Positives = 182/298 (61%), Gaps = 4/298 (1%) Query: 39 NADFPGKPITLIVPYXXXXXXXXXXXXXXXXXEKVLGQPVIVVNRTGGGGSVGLMEGAGA 98 ++D+P KPI LIVPY + LGQ V V N TGG G+VG +G + Sbjct: 37 DSDYPKKPIQLIVPYAAGGGTDTVARAIGNEVQNELGQSVAVQNVTGGSGAVGFQQGINS 96 Query: 99 KGDGYTVTFLPAELTILPHLGLLPITYEKFKPIAQTNFDPSAITVRQGAPWQTVNEFLGF 158 K DGYT+T EL LP LG+ Y F+PIA N DP+AITVR APW T+ +F+ + Sbjct: 97 KADGYTLTMTTVELLTLPQLGVSNFKYTDFRPIALLNKDPAAITVRADAPWDTIEDFIEY 156 Query: 159 AKAHPGELKMGNAGTGSIWHLAAITLERETGVKFAHIPFEGAGPAVSALMDGFVDAVPVS 218 AK + ++K+GN+G G+IWH+AAI + ETG KF H P++GA PAV+AL+ G VDAV VS Sbjct: 157 AKKN--DVKVGNSGVGAIWHMAAIAFQNETGAKFVHTPYDGAAPAVTALLGGHVDAVTVS 214 Query: 219 PAEVKKYVDEGKLRTLAVNADERSDALPGVPTLEELTGIHVNFTGTWRGLAVPKDTPDAI 278 P EV V G + LAV +DER LP VPTL+E GI ++ GTWRG+ VPK+TPD I Sbjct: 215 PGEVYTQVQSGDFKVLAVMSDERVKQLPDVPTLKE-KGIDLSI-GTWRGICVPKETPDDI 272 Query: 279 ADILAGAFIKGTEDKAFREYMNVNGLGLLVKDGKAFSRQLKESDDTFAKMIPELGLSR 336 L GAFIK +E A++ + LG + FS+ +++ + F ++ + +++ Sbjct: 273 CSKLEGAFIKASETDAYKSTLEKLNLGYASMKSEEFSKFMEQQNTQFEELSKSIDINK 330 >B7LU06.1 B7LU06_ESCF3 Putative periplasmic binding protein Length = 324 Score = 248 bits (634), Expect = 4e-64, Method: Compositional matrix adjust. Identities = 128/297 (43%), Positives = 185/297 (62%), Gaps = 2/297 (0%) Query: 40 ADFPGKPITLIVPYXXXXXXXXXXXXXXXXXEKVLGQPVIVVNRTGGGGSVGLMEGAGAK 99 A +P K I L+VPY + L + V+N+ GGGG++GL E A A+ Sbjct: 30 ASWPTKQIELVVPYAAGGGTDLVARAFADAAKNHLPVSIGVINKPGGGGAIGLSEIAAAR 89 Query: 100 GDGYTVTFLPAELTILPHLGLLPITYEKFKPIAQTNFDPSAITVRQGAPWQTVNEFLGFA 159 +GY + ELT LP LG++ FKPIA+ N DP+AITVR APW + EF+ +A Sbjct: 90 PNGYKIGLGTVELTTLPSLGMVRFKTSDFKPIARLNADPAAITVRSDAPWNSYEEFMTYA 149 Query: 160 KAHPGELKMGNAGTGSIWHLAAITLERETGVKFAHIPFEGAGPAVSALMDGFVDAVPVSP 219 KA+PG++++GN+GTG+IWHLAA LE +TG KF+H+P++GA PA++ L+ G ++AV VSP Sbjct: 150 KANPGKVRIGNSGTGAIWHLAAAALEDKTGAKFSHVPYDGAAPAITGLLGGHIEAVSVSP 209 Query: 220 AEVKKYVDEGKLRTLAVNADERSDALPGVPTLEELTGIHVNFTGTWRGLAVPKDTPDAIA 279 EV +V GKL+TL V ADER +P VPTL+E G+ ++ GTWRGL V + TP + Sbjct: 210 GEVINHVKGGKLKTLVVMADERMKTMPDVPTLKE-KGVDLSI-GTWRGLIVSQKTPQDVV 267 Query: 280 DILAGAFIKGTEDKAFREYMNVNGLGLLVKDGKAFSRQLKESDDTFAKMIPELGLSR 336 D+LA A + E+ AF++ + L DG +F Q+ E + F +++ LGL + Sbjct: 268 DVLAKAAKETAEEPAFQDALQKLNLNYAWLDGASFQTQINEQEKYFDELLTRLGLKK 324 >Q0TAM6.1 Q0TAM6_ECOL5 Putative uncharacterized protein Length = 324 Score = 248 bits (632), Expect = 7e-64, Method: Compositional matrix adjust. Identities = 127/297 (42%), Positives = 185/297 (62%), Gaps = 2/297 (0%) Query: 40 ADFPGKPITLIVPYXXXXXXXXXXXXXXXXXEKVLGQPVIVVNRTGGGGSVGLMEGAGAK 99 A +P K I L+VPY + L + V+N+ GGGG++GL E A A+ Sbjct: 30 ASYPTKQIELVVPYAAGGGTDLVARAFADAAKNHLPVSIGVINKPGGGGAIGLSEIAAAR 89 Query: 100 GDGYTVTFLPAELTILPHLGLLPITYEKFKPIAQTNFDPSAITVRQGAPWQTVNEFLGFA 159 +GY + ELT LP LG++ FKPIA+ N DP+AITVR APW + EF+ +A Sbjct: 90 PNGYKIGLGTVELTTLPSLGMVRFKTSDFKPIARLNADPAAITVRADAPWNSYEEFMTYA 149 Query: 160 KAHPGELKMGNAGTGSIWHLAAITLERETGVKFAHIPFEGAGPAVSALMDGFVDAVPVSP 219 KA+PG++++GN+GTG+IWHLAA LE +TG KF+H+P++GA PA++ L+ G ++AV VSP Sbjct: 150 KANPGKVRIGNSGTGAIWHLAAAALEDKTGAKFSHVPYDGAAPAITGLLGGHIEAVSVSP 209 Query: 220 AEVKKYVDEGKLRTLAVNADERSDALPGVPTLEELTGIHVNFTGTWRGLAVPKDTPDAIA 279 EV +V+ GKL+TL V ADER +P VPTL+E G+ ++ GTWRGL V + TP + Sbjct: 210 GEVINHVNGGKLKTLVVMADERMKTMPDVPTLKE-KGVDLSI-GTWRGLIVSQKTPQDVV 267 Query: 280 DILAGAFIKGTEDKAFREYMNVNGLGLLVKDGKAFSRQLKESDDTFAKMIPELGLSR 336 D+LA A + E+ AF++ + L D +F Q+ E + F +++ LGL + Sbjct: 268 DVLAKAAKETAEEPAFQDALQKLNLNYAWLDAASFQTQISEQEKYFDELLTRLGLKK 324 >B3HXB1.1 B3HXB1_ECOLX Periplasmic binding protein Length = 324 Score = 248 bits (632), Expect = 7e-64, Method: Compositional matrix adjust. Identities = 127/297 (42%), Positives = 185/297 (62%), Gaps = 2/297 (0%) Query: 40 ADFPGKPITLIVPYXXXXXXXXXXXXXXXXXEKVLGQPVIVVNRTGGGGSVGLMEGAGAK 99 A +P K I L+VPY + L + V+N+ GGGG++GL E A A+ Sbjct: 30 ASYPTKQIELVVPYAAGGGTDLVARAFADAAKNHLPVSIGVINKPGGGGAIGLSEIAAAR 89 Query: 100 GDGYTVTFLPAELTILPHLGLLPITYEKFKPIAQTNFDPSAITVRQGAPWQTVNEFLGFA 159 +GY + ELT LP LG++ FKPIA+ N DP+AITVR APW + EF+ +A Sbjct: 90 PNGYKIGLGTVELTTLPSLGMVRFKTSDFKPIARLNADPAAITVRADAPWNSYEEFMTYA 149 Query: 160 KAHPGELKMGNAGTGSIWHLAAITLERETGVKFAHIPFEGAGPAVSALMDGFVDAVPVSP 219 KA+PG++++GN+GTG+IWHLAA LE +TG KF+H+P++GA PA++ L+ G ++AV VSP Sbjct: 150 KANPGKVRIGNSGTGAIWHLAAAALEDKTGAKFSHVPYDGAAPAITGLLGGHIEAVSVSP 209 Query: 220 AEVKKYVDEGKLRTLAVNADERSDALPGVPTLEELTGIHVNFTGTWRGLAVPKDTPDAIA 279 EV +V+ GKL+TL V ADER +P VPTL+E G+ ++ GTWRGL V + TP + Sbjct: 210 GEVINHVNGGKLKTLVVMADERMKTMPDVPTLKE-KGVDLSI-GTWRGLIVSQKTPQDVV 267 Query: 280 DILAGAFIKGTEDKAFREYMNVNGLGLLVKDGKAFSRQLKESDDTFAKMIPELGLSR 336 D+LA A + E+ AF++ + L D +F Q+ E + F +++ LGL + Sbjct: 268 DVLAKAAKETAEEPAFQDALQKLNLNYAWLDAASFQTQISEQEKYFDELLTRLGLKK 324 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.139 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,087,960,734 Number of extensions: 94160385 Number of successful extensions: 246119 Number of sequences better than 1.0e-05: 2808 Number of HSP's gapped: 240363 Number of HSP's successfully gapped: 2808 Length of query: 337 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 201 Effective length of database: 1,779,438,163 Effective search space: 357667070763 Effective search space used: 357667070763 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)