BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0563.0008.p (364 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K4B0.1 B3K4B0_9BACI Asparaginase EC=3.5.1.1 374 e-102 B1DH86.1 B1DH86_9BACL Asparaginase EC=3.5.1.1 338 4e-91 B1DH85.1 B1DH85_9BACL Asparaginase EC=3.5.1.1 335 5e-90 Q1VD62.1 Q1VD62_VIBAL Periplasmic L-asparaginase II 162 4e-38 A7JZK4.1 A7JZK4_9VIBR L-asparaginases, type II subfamily EC=3.5.1.1 162 6e-38 >B3K4B0.1 B3K4B0_9BACI Asparaginase EC=3.5.1.1 Length = 406 Score = 374 bits (961), Expect = e-102, Method: Compositional matrix adjust. Identities = 192/332 (57%), Positives = 228/332 (68%), Gaps = 1/332 (0%) Query: 28 PKIQVIATGGTIAGQSVDETSFQNYKAGTLLIEDMVKALPNKDQIAEVSSYQFGNAXXXX 87 P + V+ATGGT+AGQS D TSFQ+Y+AGTL IEDMV LPN D+IA++S+ QFGN+ Sbjct: 76 PNVLVVATGGTLAGQSTDATSFQSYRAGTLKIEDMVGELPNTDKIADISTLQFGNSGSSA 135 Query: 88 XXXXXXXXXXLKVDEALKTQXXXXXXXXXXXMEEIAYFLDLTVRSPKPVVITGSMRPWTV 147 VD+AL+ MEEIAYFLDLTV+S KPVVITGSMRPWTV Sbjct: 136 YTMADLYDLSRTVDKALEVYDSVVVTTGTDTMEEIAYFLDLTVQSDKPVVITGSMRPWTV 195 Query: 148 IGSDAQANLYNAIKLAASGKTKYFGTVLMLNDEFHTAREVTKSNAYRTDTFETPKLGMLG 207 IGSDAQANLYNAIKLA SG+T+ FGTVLMLND H AR VTK+N YRTDTFETP LG +G Sbjct: 196 IGSDAQANLYNAIKLAGSGRTESFGTVLMLNDTIHLARGVTKTNDYRTDTFETPMLGAVG 255 Query: 208 YIDEKNIRIYRSNARSLMSEDKWATPFDLSKISPSSLAKVEIAYSYQDAGAGAITGFVED 267 YID++NIRIYR+ R++ E + FDLSKI+ LAKVEIA SYQ+AG GAI GFV D Sbjct: 256 YIDDENIRIYRAPLRAMKDEGQGKPVFDLSKITKGDLAKVEIAVSYQEAGGGAIKGFVAD 315 Query: 268 XXXXXXXXXXXXXXXSKAMGEARTKAIKEKGVIFVATTRTGSGSNYTSADGIIAGDNLNA 327 S+ M AR +AI EKGV+FV TTRTGSGS Y +GII+GDNL+ Sbjct: 316 GAKGIVTSGTGAGGISREMSAARKEAI-EKGVVFVTTTRTGSGSVYGGGEGIISGDNLSP 374 Query: 328 AHARIXXXXXXXXXXDFNTIKGWFDTYGSGQI 359 AR+ DF+TIKGWF TYG+ ++ Sbjct: 375 QQARVLLLLSLSFTDDFDTIKGWFATYGTPEV 406 >B1DH86.1 B1DH86_9BACL Asparaginase EC=3.5.1.1 Length = 816 Score = 338 bits (867), Expect = 4e-91, Method: Compositional matrix adjust. Identities = 181/336 (53%), Positives = 218/336 (64%), Gaps = 5/336 (1%) Query: 27 LPKIQVIATGGTIAGQSV--DETSFQNYKAGTLLIEDMVKALPNKDQIAEVSSYQFGNAX 84 LP I V+ATGGTIAG + D+T+FQNY AGT + DMV LP A+VS++QFGN Sbjct: 57 LPNIMVVATGGTIAGAASRGDKTNFQNYAAGTYKMADMVAQLPTHKN-ADVSTFQFGNKG 115 Query: 85 XXXXXXXXXXXXXLKVDEALKTQXXXXXXXXXXXMEEIAYFLDLTVRSPKPVVITGSMRP 144 L VD+AL+ MEEIAYFLDLTVRS KPVV+TG+MRP Sbjct: 116 SGGYSMKDLYDLSLAVDQALEVYDGVVVTTGTDTMEEIAYFLDLTVRSEKPVVVTGAMRP 175 Query: 145 WTVIGSDAQANLYNAIKLAASGKTKYFGTVLMLNDEFHTAREVTKSNAYRTDTFETPKLG 204 W VIG+D ANLY AIK+AAS KTK++GTV+MLND AREVTKSNA+R DTF+TP G Sbjct: 176 WDVIGTDGPANLYQAIKVAASNKTKWYGTVIMLNDVIQAAREVTKSNAHRLDTFDTPMFG 235 Query: 205 MLGYIDEKNIRIYRSNARSLMS-EDKWATPFDLSKISPSSLAKVEIAYSYQDAGAGAITG 263 LGY+D+ +R+YR NAR+L + +W+TPFDL IS L VEIAYSYQ+AG GAI Sbjct: 236 ALGYVDDPAVRMYRLNARALKAGTPEWSTPFDLRTISKDDLPLVEIAYSYQEAGGGAIRA 295 Query: 264 FVEDXXXXXXXXXXXXXXXSKAMGEARTKAIKEKGVIFVATTRTGSGSNYTSADGIIAGD 323 VED S M +AR+ AI +KGVIFV TTRTGSG+ S++GIIAGD Sbjct: 296 LVEDGAKGIVTAGTGAGGISSKMSQARSAAI-QKGVIFVTTTRTGSGTMSGSSNGIIAGD 354 Query: 324 NLNAAHARIXXXXXXXXXXDFNTIKGWFDTYGSGQI 359 NLN HAR+ DFNTIK WF+T G+ I Sbjct: 355 NLNPQHARMMLLLSLAFSNDFNTIKDWFETVGAQDI 390 >B1DH85.1 B1DH85_9BACL Asparaginase EC=3.5.1.1 Length = 459 Score = 335 bits (858), Expect = 5e-90, Method: Compositional matrix adjust. Identities = 179/337 (53%), Positives = 218/337 (64%), Gaps = 6/337 (1%) Query: 28 PKIQVIATGGTIAG--QSVDETSFQNYKAGTLLIEDMVKALPNKDQIAEVSSYQFGNAXX 85 P + VIATGGTIAG ++ D+T+FQNY AGT + DMV LP + A+VS++QFGN Sbjct: 62 PNVLVIATGGTIAGAARNGDKTNFQNYAAGTYTMADMVSQLPVH-KTADVSTFQFGNKGS 120 Query: 86 XXXXXXXXXXXXLKVDEALKTQXXXXXXXXXXXMEEIAYFLDLTVRSPKPVVITGSMRPW 145 L VD AL MEEIAYFLDLTVRS KPVV+TG+MRPW Sbjct: 121 GSYSIKDLYDLSLAVDTALADYDSVVVTTGTDTMEEIAYFLDLTVRSEKPVVVTGAMRPW 180 Query: 146 TVIGSDAQANLYNAIKLAASGKTKYFGTVLMLNDEFHTAREVTKSNAYRTDTFETPKLGM 205 VIG+DA ANLY AIK+A+S KTK+FGTVLMLND + AREVTK+NA+R DTF+ P G Sbjct: 181 DVIGTDAPANLYQAIKVASSNKTKWFGTVLMLNDVIYAAREVTKTNAHRLDTFDAPMFGA 240 Query: 206 LGYIDEKNIRIYRSNARSLMS-EDKWATPFDLSKISPSSLAKVEIAYSYQDAGAGAITGF 264 LGY+D+ +RIYR+ AR++ + D WATPFDL IS SL VEIAYSYQ+AG GAI GF Sbjct: 241 LGYVDDPAVRIYRAPARAIKAGTDGWATPFDLKTISKDSLPVVEIAYSYQEAGGGAIRGF 300 Query: 265 VEDXXXXXXXXXXXXXXXSKAMGEARTKAIKEKGVIFVATTRTGSGSNYTSA-DGIIAGD 323 VED S M +AR+ AI +KGV+FV TTRTGSG+ Y S I GD Sbjct: 301 VEDGAKGIVTAGTGAGGISSKMSQARSAAI-QKGVVFVTTTRTGSGTMYDSGTSATIGGD 359 Query: 324 NLNAAHARIXXXXXXXXXXDFNTIKGWFDTYGSGQID 360 NLNAAHAR+ D TI WF ++G+G ++ Sbjct: 360 NLNAAHARMLLLLSLAFTSDLPTIHSWFQSFGTGDVE 396 >Q1VD62.1 Q1VD62_VIBAL Periplasmic L-asparaginase II Length = 354 Score = 162 bits (410), Expect = 4e-38, Method: Compositional matrix adjust. Identities = 113/338 (33%), Positives = 166/338 (49%), Gaps = 20/338 (5%) Query: 27 LPKIQVIATGGTIAG--QSVDETSFQNYKAGTLLIEDMVKALPNKDQIAEVSSYQFGNAX 84 LP I+++ATGGTIAG QS E+ NY AG + +E ++ A+P+ +IA++S Q + Sbjct: 27 LPNIKILATGGTIAGAGQSATES---NYTAGKVGVESLISAVPSMTKIADISGEQVVSIG 83 Query: 85 XXXXXXXXXXXXXLKVDEALKTQXX--XXXXXXXXXMEEIAYFLDLTVRSPKPVVITGSM 142 +V+E L +EE AYFLDLTV+S KPVVI G+M Sbjct: 84 SQDMNDEVWLKLSKRVNELLDQDDVDGIVITHGTDTLEETAYFLDLTVKSKKPVVIVGAM 143 Query: 143 RPWTVIGSDAQANLYNAIKLAASGKTKYFGTVLMLNDEFHTAREVTKSNAYRTDTFETPK 202 RP T + +D NLYNA+ A ++ G ++ +ND AR+VTK+N TF++P Sbjct: 144 RPSTAMSADGPVNLYNAVVTATDEDSEGRGVLVAMNDTIFDARDVTKTNTTSVSTFQSPN 203 Query: 203 LGMLGYIDEKNIRIYRSNARSLMSEDKWATPFDLSKISPSSLAKVEIAYSYQDAGAGAIT 262 G +GYI + + RS R +E TPFD+SK+ + L KV I Y+Y +A + Sbjct: 204 FGAIGYIHNSDAKYQRSPERKHTTE----TPFDVSKL--TKLPKVGIVYNYANASNLPVK 257 Query: 263 GFVEDXXXXXXXXXXXXXXXSKAMGEARTKAIKEKGVIFVATTRTGSGSNYTSAD----- 317 FV+ + + KA K+ GV+ V ++RT SGS A+ Sbjct: 258 AFVDAKFDGIVSAGVGNGNLYHTVFDELEKASKD-GVVVVRSSRTPSGSTTLDAEIDDEK 316 Query: 318 -GIIAGDNLNAAHARIXXXXXXXXXXDFNTIKGWFDTY 354 G +A LN ARI ++ I+ F Y Sbjct: 317 YGFVAAGTLNPQKARILLMLSLTQTKNYQDIQKMFQYY 354 >A7JZK4.1 A7JZK4_9VIBR L-asparaginases, type II subfamily EC=3.5.1.1 Length = 354 Score = 162 bits (409), Expect = 6e-38, Method: Compositional matrix adjust. Identities = 112/338 (33%), Positives = 167/338 (49%), Gaps = 20/338 (5%) Query: 27 LPKIQVIATGGTIAG--QSVDETSFQNYKAGTLLIEDMVKALPNKDQIAEVSSYQFGNAX 84 LP I+++ATGGTIAG QS E+ NY AG + +E ++ A+P+ IA++S Q + Sbjct: 27 LPNIKILATGGTIAGAGQSATES---NYTAGKVGVESLISAVPSMTNIADISGEQVVSIG 83 Query: 85 XXXXXXXXXXXXXLKVDEALKTQXX--XXXXXXXXXMEEIAYFLDLTVRSPKPVVITGSM 142 +V+E L +EE AYFLDLTV+S KPVVI G+M Sbjct: 84 SQDMNDEVWLKLAKRVNELLAQDDVDGIVITHGTDTLEETAYFLDLTVKSKKPVVIVGAM 143 Query: 143 RPWTVIGSDAQANLYNAIKLAASGKTKYFGTVLMLNDEFHTAREVTKSNAYRTDTFETPK 202 RP T + +D NLYNA+ A ++ G ++ +ND AR+VTK+N TF++P Sbjct: 144 RPSTAMSADGPVNLYNAVVTATDEDSEGRGVLVAMNDTIFDARDVTKTNTTSVSTFQSPN 203 Query: 203 LGMLGYIDEKNIRIYRSNARSLMSEDKWATPFDLSKISPSSLAKVEIAYSYQDAGAGAIT 262 G +GYI + + RS R +E TPFD+SK+ ++L KV I Y+Y +A + + Sbjct: 204 FGPIGYIHNSDAKYQRSPERKHTTE----TPFDVSKL--NTLPKVGIVYNYANASSLPVK 257 Query: 263 GFVEDXXXXXXXXXXXXXXXSKAMGEARTKAIKEKGVIFVATTRTGSGSNYTSAD----- 317 FV+ + + KA K+ GV+ V ++RT +GS A+ Sbjct: 258 AFVDAKFDGIVSAGVGNGNLYHTVFDELEKASKD-GVVVVRSSRTPTGSTTLDAEIDDDK 316 Query: 318 -GIIAGDNLNAAHARIXXXXXXXXXXDFNTIKGWFDTY 354 G +A LN ARI ++ I+ F Y Sbjct: 317 YGFVASGTLNPQKARILLMLSLTQTKNYQDIQKMFQYY 354 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.314 0.129 0.360 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,743,702,231 Number of extensions: 60644568 Number of successful extensions: 98791 Number of sequences better than 1.0e-05: 823 Number of HSP's gapped: 98139 Number of HSP's successfully gapped: 823 Length of query: 364 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 227 Effective length of database: 1,770,017,148 Effective search space: 401793892596 Effective search space used: 401793892596 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 132 (55.5 bits)