BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0563.0003.p (396 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DH74.1 B1DH74_9BACL Alkaline phosphatase EC=3.1.3.1 552 e-155 C1P781.1 C1P781_BACCO Alkaline phosphatase EC=3.1.3.1 506 e-141 C3IXW1.1 C3IXW1_9BACI Alkaline phosphatase EC=3.1.3.1 472 e-131 B7Y6B2.1 B7Y6B2_9BACI Alkaline phosphatase EC=3.1.3.1 472 e-131 Q5KWF0.1 Q5KWF0_GEOKA Alkaline phosphatase EC=3.1.3.1 472 e-131 >B1DH74.1 B1DH74_9BACL Alkaline phosphatase EC=3.1.3.1 Length = 420 Score = 552 bits (1423), Expect = e-155, Method: Compositional matrix adjust. Identities = 277/388 (71%), Positives = 309/388 (79%), Gaps = 3/388 (0%) Query: 11 AASSKTKNVILFVGDGMGTAARNAIRLATVGEKGKLAMDDMPYAGLVHTSSTVPVTDXXX 70 AA+ K KNV+LFVGDGMGTA R+AIRLATVGE G+LAMD MPY GL+HTSST +TD Sbjct: 34 AATQKAKNVVLFVGDGMGTAQRDAIRLATVGESGQLAMDSMPYVGLIHTSSTTAITDSAA 93 Query: 71 XXXXXXXGVKTYNGAIGMDANKKSVKTIAEYAKEAGKSTGVVTTSQITDATGAAFGAHVE 130 GVKTYNGAIG+DANKKSVKTI EYAK+ G STG+VTTSQITDAT AAF +HVE Sbjct: 94 SATAYASGVKTYNGAIGVDANKKSVKTIMEYAKDKGMSTGLVTTSQITDATPAAFASHVE 153 Query: 131 DRSKQSDIALQLLTKSKVDVLLGGGEDFWYPAGEAGKFPDAPAEDPSEKSKGTQGNLVTK 190 DRSKQSDIALQ LTKSKVDVLLGGGEDFWYPAG G F D DPSEKSKGTQG+LV K Sbjct: 154 DRSKQSDIALQYLTKSKVDVLLGGGEDFWYPAGNKGAFDDETPADPSEKSKGTQGDLVKK 213 Query: 191 AKQLGYNYVSTXXXXXXXXXXXXXXXFANEEMFQQNEEGKGDIYNPVVSLPEMTKKAIDT 250 AK LGY YV T FANEEMFQQ EG+GD+YNP+VSLP+MTKKA+DT Sbjct: 214 AKSLGYTYV-TNKTDMTKAKGKLLGLFANEEMFQQKPEGQGDVYNPIVSLPDMTKKALDT 272 Query: 251 LSTNQKGFFLMVEEEGTDEFAHKNNAKMTIKSGQALDASVQVAKDFAKKNPDTLVLVLAD 310 LS N+KGFFLMVEEE TDE AH NNAK+TIK+GQ LD SV+VAKDFAK++PDTLVLVLAD Sbjct: 273 LSANKKGFFLMVEEEATDEMAHNNNAKLTIKAGQELDKSVKVAKDFAKQHPDTLVLVLAD 332 Query: 311 HETGGLSIEPVDAEDETGDG--ISQEDGPFAIANSKESFVVDWTTSGHTAVDIPVTAMGK 368 HETGG S+E VD DE+ DG IS EDGPFAIA SK +FVVDWTTSGHT VD+PVTA G Sbjct: 333 HETGGFSVEEVDDSDESTDGKTISGEDGPFAIAGSKMNFVVDWTTSGHTGVDVPVTATGA 392 Query: 369 NAQLFTGVFENTAVFDKLMQAYGFNAKK 396 ++LFTG+FENT++F+KL QA KK Sbjct: 393 GSELFTGIFENTSIFNKLAQALNITIKK 420 >C1P781.1 C1P781_BACCO Alkaline phosphatase EC=3.1.3.1 Length = 423 Score = 506 bits (1304), Expect = e-141, Method: Compositional matrix adjust. Identities = 250/390 (64%), Positives = 296/390 (75%), Gaps = 4/390 (1%) Query: 7 KPAQ----AASSKTKNVILFVGDGMGTAARNAIRLATVGEKGKLAMDDMPYAGLVHTSST 62 KP+Q A S+KTKNVILFVGDGMG A R+AIRLA+ G G L M+ MPY G+VHTSST Sbjct: 32 KPSQHTGHATSAKTKNVILFVGDGMGAAQRDAIRLASTGLSGDLEMNKMPYTGVVHTSST 91 Query: 63 VPVTDXXXXXXXXXXGVKTYNGAIGMDANKKSVKTIAEYAKEAGKSTGVVTTSQITDATG 122 VTD GVKTYNGAIG+DANK S KT+ E AK+AGK+TG+VTTSQITDAT Sbjct: 92 SVVTDSAAAATAMASGVKTYNGAIGVDANKVSKKTVLEEAKQAGKATGLVTTSQITDATP 151 Query: 123 AAFGAHVEDRSKQSDIALQLLTKSKVDVLLGGGEDFWYPAGEAGKFPDAPAEDPSEKSKG 182 AAFGAHV++RS+QS IA Q L SKVDVLLGGGED+WYP G G + D PA+DPSE SKG Sbjct: 152 AAFGAHVKNRSEQSKIAKQYLENSKVDVLLGGGEDYWYPKGSQGAYEDHPAKDPSEASKG 211 Query: 183 TQGNLVTKAKQLGYNYVSTXXXXXXXXXXXXXXXFANEEMFQQNEEGKGDIYNPVVSLPE 242 T+GNLV+KAK+LGY YV+ FANEEMF+Q EEG GD Y+PVVSLP+ Sbjct: 212 TEGNLVSKAKRLGYQYVTNASGLKKAGGNKLLGLFANEEMFEQREEGHGDQYDPVVSLPQ 271 Query: 243 MTKKAIDTLSTNQKGFFLMVEEEGTDEFAHKNNAKMTIKSGQALDASVQVAKDFAKKNPD 302 MT+KAI TLS N+KGFFLMVEEE DE AH+NNA IK+G+ LD +V+ AKD+AKK+PD Sbjct: 272 MTEKAISTLSKNKKGFFLMVEEEAIDEMAHENNASQLIKAGRQLDNAVKAAKDYAKKHPD 331 Query: 303 TLVLVLADHETGGLSIEPVDAEDETGDGISQEDGPFAIANSKESFVVDWTTSGHTAVDIP 362 TLVLVLADH+ GG+++E V+ DE+GDGIS+EDGPF +A S E FVVDWTTSGHTA DIP Sbjct: 332 TLVLVLADHDCGGMTVENVEHSDESGDGISKEDGPFQVAGSSEQFVVDWTTSGHTAADIP 391 Query: 363 VTAMGKNAQLFTGVFENTAVFDKLMQAYGF 392 +TA G A LF G +ENT + D L++A G Sbjct: 392 LTATGSGASLFEGTYENTYIHDALLRAMGL 421 >C3IXW1.1 C3IXW1_9BACI Alkaline phosphatase EC=3.1.3.1 Length = 426 Score = 472 bits (1215), Expect = e-131, Method: Compositional matrix adjust. Identities = 237/387 (61%), Positives = 286/387 (73%), Gaps = 7/387 (1%) Query: 5 PAKPAQAASSKTKNVILFVGDGMGTAARNAIRLATVGEKGKLAMDDMPYAGLVHTSSTVP 64 PAK Q+ S+ KNV+LFVGDGMGTA RNAIRLAT G G+L MDDMPY+GLVHT+S Sbjct: 32 PAK--QSKPSEVKNVVLFVGDGMGTAHRNAIRLATKGIAGELEMDDMPYSGLVHTNSADS 89 Query: 65 VT---DXXXXXXXXXXGVKTYNGAIGMDANKKSVKTIAEYAKEAGKSTGVVTTSQITDAT 121 + D GVKTYNGAI +D +K V+TI E AK+AGK+TG+VTT+Q+TDAT Sbjct: 90 KSFITDSAAAATAIASGVKTYNGAISVDLQRKPVETILEQAKKAGKATGLVTTAQVTDAT 149 Query: 122 GAAFGAHVEDRSKQSDIALQLLTKSKVDVLLGGGEDFWYPAGEAGKFPDAPAEDPSEKSK 181 AAF AH +RS QSDIA Q + ++KVDV+LGGGED+WYPAG G +PD PAEDP E SK Sbjct: 150 PAAFAAHTANRSAQSDIAKQYIEQTKVDVILGGGEDYWYPAGNPGYYPDHPAEDPEEASK 209 Query: 182 GTQGNLVTKAKQLGYNYVSTXXXXXXXXXXXXXXXFANEEMFQQNEEGKGDIYNPVVSLP 241 GTQGNLV +AKQLGY YV T FANEEMFQQ EG+GD+YNPVV LP Sbjct: 210 GTQGNLVERAKQLGYTYVRTADELKQASGRKLLGLFANEEMFQQRPEGEGDVYNPVVPLP 269 Query: 242 EMTKKAIDTLSTNQKGFFLMVEEEGTDEFAHKNNAKMTIKSGQALDASVQVAKDFAKKNP 301 +MTKKA+D LS N+KGFFL+VEEE DE +H+NN + IK+GQ LD +V VAK +AK++ Sbjct: 270 DMTKKALDVLSQNKKGFFLVVEEEAIDEMSHENNGSLMIKAGQQLDEAVAVAKQYAKRHR 329 Query: 302 DTLVLVLADHETGGLSIEPVDAEDETGDG--ISQEDGPFAIANSKESFVVDWTTSGHTAV 359 DTLVLVLADHE GGL+IE DE+GDG IS EDGPF IA S ++F +DWTT+GHTA Sbjct: 330 DTLVLVLADHECGGLTIETPGGTDESGDGKTISGEDGPFPIARSMQTFALDWTTTGHTAA 389 Query: 360 DIPVTAMGKNAQLFTGVFENTAVFDKL 386 D+P+TAMG A+ FTG++ENT + D L Sbjct: 390 DVPLTAMGPGAERFTGIYENTRIHDIL 416 >B7Y6B2.1 B7Y6B2_9BACI Alkaline phosphatase EC=3.1.3.1 Length = 426 Score = 472 bits (1215), Expect = e-131, Method: Compositional matrix adjust. Identities = 237/387 (61%), Positives = 286/387 (73%), Gaps = 7/387 (1%) Query: 5 PAKPAQAASSKTKNVILFVGDGMGTAARNAIRLATVGEKGKLAMDDMPYAGLVHTSSTVP 64 PAK Q+ S+ KNV+LFVGDGMGTA RNAIRLAT G G+L MDDMPY+GLVHT+S Sbjct: 32 PAK--QSKPSEVKNVVLFVGDGMGTAHRNAIRLATKGIAGELEMDDMPYSGLVHTNSADS 89 Query: 65 VT---DXXXXXXXXXXGVKTYNGAIGMDANKKSVKTIAEYAKEAGKSTGVVTTSQITDAT 121 + D GVKTYNGAI +D +K V+TI E AK+AGK+TG+VTT+Q+TDAT Sbjct: 90 KSFITDSAAAATAIASGVKTYNGAISVDLQRKPVETILEQAKKAGKATGLVTTAQVTDAT 149 Query: 122 GAAFGAHVEDRSKQSDIALQLLTKSKVDVLLGGGEDFWYPAGEAGKFPDAPAEDPSEKSK 181 AAF AH +RS QSDIA Q + ++KVDV+LGGGED+WYPAG G +PD PAEDP E SK Sbjct: 150 PAAFAAHTANRSAQSDIAKQYIEQTKVDVILGGGEDYWYPAGNPGYYPDHPAEDPEEASK 209 Query: 182 GTQGNLVTKAKQLGYNYVSTXXXXXXXXXXXXXXXFANEEMFQQNEEGKGDIYNPVVSLP 241 GTQGNLV +AKQLGY YV T FANEEMFQQ EG+GD+YNPVV LP Sbjct: 210 GTQGNLVERAKQLGYTYVRTADELKQASGRKLLGLFANEEMFQQRPEGEGDVYNPVVPLP 269 Query: 242 EMTKKAIDTLSTNQKGFFLMVEEEGTDEFAHKNNAKMTIKSGQALDASVQVAKDFAKKNP 301 +MTKKA+D LS N+KGFFL+VEEE DE +H+NN + IK+GQ LD +V VAK +AK++ Sbjct: 270 DMTKKALDVLSQNKKGFFLVVEEEAIDEMSHENNGSLMIKAGQQLDEAVAVAKQYAKRHR 329 Query: 302 DTLVLVLADHETGGLSIEPVDAEDETGDG--ISQEDGPFAIANSKESFVVDWTTSGHTAV 359 DTLVLVLADHE GGL+IE DE+GDG IS EDGPF IA S ++F +DWTT+GHTA Sbjct: 330 DTLVLVLADHECGGLTIETPGGTDESGDGKTISGEDGPFPIARSMQTFALDWTTTGHTAA 389 Query: 360 DIPVTAMGKNAQLFTGVFENTAVFDKL 386 D+P+TAMG A+ FTG++ENT + D L Sbjct: 390 DVPLTAMGPGAERFTGIYENTRIHDIL 416 >Q5KWF0.1 Q5KWF0_GEOKA Alkaline phosphatase EC=3.1.3.1 Length = 426 Score = 472 bits (1214), Expect = e-131, Method: Compositional matrix adjust. Identities = 238/389 (61%), Positives = 286/389 (73%), Gaps = 7/389 (1%) Query: 5 PAKPAQAASSKTKNVILFVGDGMGTAARNAIRLATVGEKGKLAMDDMPYAGLVHTSSTVP 64 PAK Q+ S+ KNV+LFVGDGMGTA RNAIRLAT G G+L MDDMPY+GLVHT+S Sbjct: 32 PAK--QSKPSEVKNVVLFVGDGMGTAHRNAIRLATKGIAGELEMDDMPYSGLVHTNSADS 89 Query: 65 VT---DXXXXXXXXXXGVKTYNGAIGMDANKKSVKTIAEYAKEAGKSTGVVTTSQITDAT 121 + D GVKTYNGAI +D K V+TI E AK+AGK+TG+VTT+Q+TDAT Sbjct: 90 KSFITDSAAAATAIASGVKTYNGAISVDLQGKPVETILEQAKKAGKATGLVTTAQVTDAT 149 Query: 122 GAAFGAHVEDRSKQSDIALQLLTKSKVDVLLGGGEDFWYPAGEAGKFPDAPAEDPSEKSK 181 AAF AH +RS QSDIA Q + ++KVDV+LGGGED+WYPAG G +PD PAEDP E SK Sbjct: 150 PAAFAAHTANRSAQSDIAKQYIEQTKVDVILGGGEDYWYPAGNPGYYPDHPAEDPEEASK 209 Query: 182 GTQGNLVTKAKQLGYNYVSTXXXXXXXXXXXXXXXFANEEMFQQNEEGKGDIYNPVVSLP 241 GTQGNLV +AKQLGY YV T FANEEMFQQ EG+GD+YNPVV LP Sbjct: 210 GTQGNLVERAKQLGYTYVRTADELKQASGRKLLGLFANEEMFQQRPEGEGDVYNPVVPLP 269 Query: 242 EMTKKAIDTLSTNQKGFFLMVEEEGTDEFAHKNNAKMTIKSGQALDASVQVAKDFAKKNP 301 +MTKKA+D LS N+KGFFL+VEEE DE +H+NN + IK+GQ LD +V VAK +AK++ Sbjct: 270 DMTKKALDVLSQNKKGFFLVVEEEAIDEMSHENNGSLMIKAGQQLDEAVAVAKQYAKRHR 329 Query: 302 DTLVLVLADHETGGLSIEPVDAEDETGDG--ISQEDGPFAIANSKESFVVDWTTSGHTAV 359 DTLVLVLADHE GGL+IE DE+GDG IS EDGPF IA S ++F +DWTT+GHTA Sbjct: 330 DTLVLVLADHECGGLTIETPGGTDESGDGKTISGEDGPFPIARSTQTFALDWTTTGHTAA 389 Query: 360 DIPVTAMGKNAQLFTGVFENTAVFDKLMQ 388 D+P+TAMG A+ FTG++ENT + D L Q Sbjct: 390 DVPLTAMGPGAERFTGIYENTRIHDILEQ 418 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.310 0.127 0.353 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,172,583,934 Number of extensions: 86525410 Number of successful extensions: 181446 Number of sequences better than 1.0e-05: 1172 Number of HSP's gapped: 178941 Number of HSP's successfully gapped: 1536 Length of query: 396 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 258 Effective length of database: 1,760,596,133 Effective search space: 454233802314 Effective search space used: 454233802314 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.7 bits) S2: 132 (55.5 bits)