BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0562.0008.p (172 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B8GGH6.1 B8GGH6_METPE Transcriptional regulator, TetR family 116 9e-25 B9E1V2.1 B9E1V2_CLOK1 Putative uncharacterized protein 100 7e-20 A1THL6.1 A1THL6_MYCVP Transcriptional regulator, TetR family 99 1e-19 C4BDH8.1 C4BDH8_9MICO Transcriptional regulator, COG1309 99 1e-19 B8EQU1.1 B8EQU1_METSB Transcriptional regulator, TetR family 99 1e-19 >B8GGH6.1 B8GGH6_METPE Transcriptional regulator, TetR family Length = 215 Score = 116 bits (290), Expect = 9e-25, Method: Compositional matrix adjust. Identities = 68/154 (44%), Positives = 94/154 (61%), Gaps = 6/154 (3%) Query: 2 STSQEQDKLKTRRRGKELEAAILQAVREELKERGYSNLTMDGVAERSGTSKAVLYRRWAN 61 ST E+ RRRG+ LEAAILQA +EL + GY++LTM+GVA R+ T+KAVLYRRWAN Sbjct: 15 STGDERRHTGNRRRGEILEAAILQAAWDELFDGGYTHLTMEGVAARARTNKAVLYRRWAN 74 Query: 62 RAEIVLAAIRERVPLPLEEVPDYGNLRDDVCGVLRSMNQNNIEVMMKAFYGLVTE----- 116 +A++V AAI + VP P +VPD G+LR+DV +L + Q + + +GL+ E Sbjct: 75 KAKLVAAAISKHVPRPTGDVPDTGDLRNDVLILLHGIAQPLQIIGAETMHGLMVEYLDKD 134 Query: 117 -IGAMPLASLIFPEGRNNRTMTIVLQRAVDRGEI 149 I + P E R M +L+ A RGE+ Sbjct: 135 MISSFPQRMHPGTESRLTTAMMTILKNADTRGEV 168 >B9E1V2.1 B9E1V2_CLOK1 Putative uncharacterized protein Length = 231 Score = 100 bits (248), Expect = 7e-20, Method: Compositional matrix adjust. Identities = 68/177 (38%), Positives = 96/177 (54%), Gaps = 14/177 (7%) Query: 5 QEQDKLKT--RRRGKELEAAILQAVREELKERGYSNLTMDGVAERSGTSKAVLYRRWANR 62 Q DK K RRRG+ LE AIL A EEL E GY+++TM+ +A R+GT+K+VLYRRWA++ Sbjct: 27 QNMDKQKKGKRRRGEVLEEAILNAAWEELTETGYTHMTMESIATRAGTNKSVLYRRWADK 86 Query: 63 AEIVLAAIRE-RVPLPLEEVPDYGNLRDDVCGVLRSMNQNNIEVMMKAFYGLVTE----- 116 +E+V+AA+R+ P E+P+ GNLR DV L + + + + GL+ E Sbjct: 87 SELVIAALRKYYFPKITNEIPNTGNLRGDVYAYLHARVEPLKTIGTETIRGLMMEPLVWR 146 Query: 117 --IGAMPLASLIFPEGRNNRTMTIVLQRAVDRGEISAEAXX----XLPRGCLLYHQI 167 +MP E + M ++L+ A RGEI E LP L Y I Sbjct: 147 MITASMPQMIERRSESKLTAAMAVILKNAELRGEIRLEKLTPRIISLPVDLLQYELI 203 >A1THL6.1 A1THL6_MYCVP Transcriptional regulator, TetR family Length = 213 Score = 99.4 bits (246), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 59/144 (40%), Positives = 84/144 (58%), Gaps = 7/144 (4%) Query: 13 RRRGKELEAAILQAVREELKERGYSNLTMDGVAERSGTSKAVLYRRWANRAEIVLAAIRE 72 RRRG LE+AIL A E+L E GY T+D VA RSG++++VLYRRW +R +++ A IR Sbjct: 10 RRRGAALESAILDAGWEQLIEAGYERFTIDAVASRSGSARSVLYRRWPSRLDLLKAVIRH 69 Query: 73 RVPLPLEEVPDYGNLRDDVCGVLRSMN---QNNIEVMMKAFYGLVTEIGAMP--LASLIF 127 R + + PD G LR DV +L + N I ++ E G P L SL Sbjct: 70 RGEVDMIPTPDTGTLRGDVLAILTAFNDRRSRTIGLLAARLGAYFDEAGGSPHELRSLFV 129 Query: 128 PEGRNNRTMTIVLQRAVDRGEISA 151 P+G + +M +++RAV RGE++A Sbjct: 130 PQGPS--SMEKIVERAVLRGELAA 151 >C4BDH8.1 C4BDH8_9MICO Transcriptional regulator, COG1309 Length = 195 Score = 99.4 bits (246), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 60/147 (40%), Positives = 88/147 (59%), Gaps = 7/147 (4%) Query: 12 TRRRGKELEAAILQAVREELKERGYSNLTMDGVAERSGTSKAVLYRRWANRAEIVLAAIR 71 TRRRG ELE AIL+A ++L GYS T + +AER+ T KAVLYRRW+ + ++LA I Sbjct: 4 TRRRGAELEKAILEAGWDQLLSDGYSGFTFEAIAERAQTGKAVLYRRWSGKEALLLAVIT 63 Query: 72 ERVPLPLEEVPDYGNLRDDVCGVLRSMNQ--NNIEVMMKAFYGLVTEIGAMPLASL---I 126 + +++PD G+LRDDV +LRS N ++I ++ A G + + +A L + Sbjct: 64 QHHLDDSQDLPDTGSLRDDVLELLRSRNAIGDHIAALLSAILGAHFDDTHVTIAELRRQL 123 Query: 127 FPEGRNNRTMTIVLQRAVDRGEISAEA 153 E + T+ ++QRAV RGEI A Sbjct: 124 LEE--HGPTLRTIVQRAVARGEIPGPA 148 >B8EQU1.1 B8EQU1_METSB Transcriptional regulator, TetR family Length = 204 Score = 99.0 bits (245), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 61/153 (39%), Positives = 88/153 (57%), Gaps = 3/153 (1%) Query: 3 TSQEQDKLKTRRRGKELEAAILQAVREELKERGYSNLTMDGVAERSGTSKAVLYRRWANR 62 T Q + +RRRG LE A+LQ E+L E GYSN TMD VA+RS TS+ V+YRRW++R Sbjct: 2 TDQTKRPKLSRRRGMALEKALLQVAWEQLTEGGYSNFTMDAVADRSHTSRPVIYRRWSSR 61 Query: 63 AEIVLAAIRERVPLPLEEVPDYGNLRDDVCGVL-RSMNQNNI--EVMMKAFYGLVTEIGA 119 AE+ +AAI V PD G LRD++ VL +SM + + ++ + L E A Sbjct: 62 AELAIAAIGHHVAEHPVVAPDLGRLRDEMISVLQQSMERGALIAGIIARDMSDLYRETKA 121 Query: 120 MPLASLIFPEGRNNRTMTIVLQRAVDRGEISAE 152 P + G + + +++RAV RGE+S + Sbjct: 122 TPSSLRQSILGAEGKLLEKIIERAVARGELSGQ 154 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.134 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 905,390,576 Number of extensions: 32805510 Number of successful extensions: 102726 Number of sequences better than 1.0e-05: 302 Number of HSP's gapped: 102762 Number of HSP's successfully gapped: 302 Length of query: 172 Length of database: 3,060,696,203 Length adjustment: 126 Effective length of query: 46 Effective length of database: 1,873,648,313 Effective search space: 86187822398 Effective search space used: 86187822398 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 126 (53.1 bits)