BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0562.0007.p (458 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B8GGH7.1 B8GGH7_METPE Drug resistance transporter, EmrB/QacA sub... 366 3e-99 B1DC73.1 B1DC73_9BACL Drug resistance transporter, EmrB/QacA sub... 345 5e-93 B9E1V3.1 B9E1V3_CLOK1 Putative uncharacterized protein 342 6e-92 A5N8E7.1 A5N8E7_CLOK5 Predicted transporter protein 342 6e-92 Q9RJX6.1 Q9RJX6_STRCO Putative export protein 314 1e-83 >B8GGH7.1 B8GGH7_METPE Drug resistance transporter, EmrB/QacA subfamily Length = 479 Score = 366 bits (939), Expect = 3e-99, Method: Compositional matrix adjust. Identities = 185/430 (43%), Positives = 274/430 (63%), Gaps = 2/430 (0%) Query: 12 KQSSETFEAVEQQAAAK--KMMWILMVGMLAPLFDTTITNVAIDTLVREFSTSVSTIQWV 69 K+S++ ++ K ++ I+++G LAPL D+T+ NVAI+ + + ++++S IQWV Sbjct: 3 KKSTDFVSPQREKLDPKILRVSVIMVIGALAPLLDSTMVNVAINIIAGDLNSTISIIQWV 62 Query: 70 MTSYLLALGMVIPXXXXXXXXXGGKPMWLFSLGMFLLGSVLCSLAWNVESLIFFRTIQGI 129 +T Y+LA+ + +P GGK +++FSL +F GSVL SL+WN++SLI FR +QG+ Sbjct: 63 ITGYILAMALAVPVSGWAVNRFGGKRVYMFSLFVFFAGSVLSSLSWNIDSLIGFRLLQGV 122 Query: 130 GGGLLMPIMQTLGVRAFKGENMGKMMATXXXXXXXXXXXXXXXXXXXXNHLSWRWIFLVN 189 G GL++P +QT+ V N+G++M+ N L+WRWIF VN Sbjct: 123 GAGLMIPTLQTVLVHIAGSRNLGRVMSIVSIPALLGPILGPVLGGVIVNSLNWRWIFYVN 182 Query: 190 IPICIVAIIMAWRGLPNEERGTRTLRLDLPGLMLLSPAIVLIIYGLGEVSSNHGFGHSXX 249 IPI I+A+++ WRG+P +E T LD+ G++L+SP L+IYG+ ++SS+ GF S Sbjct: 183 IPITIIALLLTWRGVPTDEPSTSKQSLDIIGILLVSPGFALLIYGISQISSHGGFTSSAV 242 Query: 250 XXXXXXXXXXXXXXXXXXFRKGKEALLDIRLFRVRSLAVSSILLFLSGLTTYGAMLLLPL 309 R ++D+RLF+ R+ + S+ LLFLSG+ T GAMLLLPL Sbjct: 243 LIPLVIGLLLMVAFIVYALRTKNAPVIDVRLFKSRNFSASTALLFLSGIITNGAMLLLPL 302 Query: 310 YFQQVRGESVLISGLLLVPQGIGMLLTRSLAGKLTDQIGSRPVVXXXXXXXXXXXWPFTQ 369 Y+QQVRGESVL +GLLL+PQG+GMLLTR+ G+L D+IGSR +V PF Sbjct: 303 YYQQVRGESVLFAGLLLIPQGVGMLLTRNWVGRLADRIGSRSIVAISLVVTMIGTVPFAC 362 Query: 370 LGADTSYYYLGAALIVRGAGLGAVFLPVMASAYIGLSSQQIPHASSTTRIMQQIGGAFGA 429 G +T+ L AL++RGAGLG + +P+MASAY GLS Q+PHASS TRI IGGAFG+ Sbjct: 363 AGPETNQILLAVALLIRGAGLGGLLIPIMASAYQGLSRDQVPHASSATRIFLTIGGAFGS 422 Query: 430 SVIAIILQNE 439 +V+AI+++++ Sbjct: 423 AVLAILIEHQ 432 >B1DC73.1 B1DC73_9BACL Drug resistance transporter, EmrB/QacA subfamily Length = 477 Score = 345 bits (886), Expect = 5e-93, Method: Compositional matrix adjust. Identities = 184/424 (43%), Positives = 259/424 (61%), Gaps = 1/424 (0%) Query: 29 KMMWILMVGMLAPLFDTTITNVAIDTLVREFSTSVSTIQWVMTSYLLALGMVIPXXXXXX 88 K+ IL++G + PL D+T+ NVAI+TL + ++VST QWV+T Y+L +G+ +P Sbjct: 22 KIALILVLGAIVPLLDSTMINVAINTLTVDLHSTVSTTQWVITGYVLTMGLAVPVSGWAI 81 Query: 89 XXXGGKPMWLFSLGMFLLGSVLCSLAWNVESLIFFRTIQGIGGGLLMPIMQTLGVRAFKG 148 GGK ++LFSL +FL+ S+LCSLAWN ESLI FR +QG G GLL+P + + V++ G Sbjct: 82 QRFGGKNLYLFSLVVFLVSSLLCSLAWNTESLIGFRLLQGAGAGLLVPTITNVLVQSAGG 141 Query: 149 ENMGKMMATXXXXXXXXXXXXXXXXXXXXNHLSWRWIFLVNIPICIVAIIMAWRGLPNEE 208 +G+++A +++SWRWIF VNIP+ +VA+ ++WR +P E Sbjct: 142 RGLGRLIALISIPTLIGPILGPVIGGLIMDNMSWRWIFYVNIPVMLVALWLSWRHIPKSE 201 Query: 209 RGTRTLRLDLPGLMLLSPAIVLIIYGLGEVSSNHGFGHSXXXXXXXXXXXXXXXXXXXXF 268 + D GL+LLSPA +IYG+ ++SS G + Sbjct: 202 AAAGRPKFDFLGLLLLSPAFAALIYGISQISS-EGLSSAGAMIPLAAGVVLTAAYIVYAL 260 Query: 269 RKGKEALLDIRLFRVRSLAVSSILLFLSGLTTYGAMLLLPLYFQQVRGESVLISGLLLVP 328 R + LLD+ LFR R S+I+LF SG+ GA+LLLPLY++QVRGESVL +GLLL+P Sbjct: 261 RTKQAPLLDLHLFRSRHFLASNIILFFSGMVMNGALLLLPLYYEQVRGESVLTTGLLLIP 320 Query: 329 QGIGMLLTRSLAGKLTDQIGSRPVVXXXXXXXXXXXWPFTQLGADTSYYYLGAALIVRGA 388 QGIGMLLTRS GKL D+ GSR +V PF G DTS+ L A +VRGA Sbjct: 321 QGIGMLLTRSWIGKLADRAGSRNIVLVSLAVTLASSIPFAFAGTDTSHIVLSVAQLVRGA 380 Query: 389 GLGAVFLPVMASAYIGLSSQQIPHASSTTRIMQQIGGAFGASVIAIILQNEYNSVMSQNP 448 + +F+P+M SAY GL +QIPHAS +TRI Q IGGAFG++V+A ++ ++ S + Sbjct: 381 AMNGIFIPIMVSAYEGLQREQIPHASISTRIFQTIGGAFGSAVLATVIGHQLKSGAGGSL 440 Query: 449 AAIA 452 AA+A Sbjct: 441 AALA 444 >B9E1V3.1 B9E1V3_CLOK1 Putative uncharacterized protein Length = 472 Score = 342 bits (876), Expect = 6e-92, Method: Compositional matrix adjust. Identities = 185/441 (41%), Positives = 264/441 (59%), Gaps = 5/441 (1%) Query: 12 KQSSETFEAVEQQAAAKKMMWILMVGMLAPLFDTTITNVAIDTLVREFSTSVSTIQWVMT 71 K + E + V +AA +L+VG LAPLFD+T+ NVAI T+ + T++ST+QWV T Sbjct: 7 KPAKEKLDPVVLRAAI-----VLVVGALAPLFDSTMVNVAIHTIAVDMKTTISTVQWVAT 61 Query: 72 SYLLALGMVIPXXXXXXXXXGGKPMWLFSLGMFLLGSVLCSLAWNVESLIFFRTIQGIGG 131 Y+LA+G+ +P G K ++FSL +F +GSV L+WN+ S+I FR IQGI Sbjct: 62 GYVLAMGLTVPISGWATKRFGCKRSYIFSLALFFIGSVCSMLSWNIGSMIIFRVIQGISA 121 Query: 132 GLLMPIMQTLGVRAFKGENMGKMMATXXXXXXXXXXXXXXXXXXXXNHLSWRWIFLVNIP 191 GL+MP++QT V+ G N+G++M+ N L+WR IFLVNIP Sbjct: 122 GLMMPVLQTELVQISGGRNLGRIMSIVSIPAMLGPILGPVLGGIIVNSLNWRAIFLVNIP 181 Query: 192 ICIVAIIMAWRGLPNEERGTRTLRLDLPGLMLLSPAIVLIIYGLGEVSSNHGFGHSXXXX 251 ICIVAI +A G+P ++ + LD+ GL+LLSPA L+IYG+ +++++ G S Sbjct: 182 ICIVAIPLALWGIPADKLLEKKASLDVIGLLLLSPAFALLIYGIAQIATHRGLNSSAVYV 241 Query: 252 XXXXXXXXXXXXXXXXFRKGKEALLDIRLFRVRSLAVSSILLFLSGLTTYGAMLLLPLYF 311 +E L++RLF+ + + S+ILL SG+ T GA+L+LPLY+ Sbjct: 242 PLIIGIVLMASYVVYALNTKREPALNLRLFKSVNFSASNILLIFSGIITNGALLMLPLYY 301 Query: 312 QQVRGESVLISGLLLVPQGIGMLLTRSLAGKLTDQIGSRPVVXXXXXXXXXXXWPFTQLG 371 Q+VRG S L +GL L+PQGIGMLLTRS AGK D+ GSR +V PF G Sbjct: 302 QEVRGASALSAGLWLLPQGIGMLLTRSWAGKAADRDGSRNIVLMSLAAIVMGTLPFAFAG 361 Query: 372 ADTSYYYLGAALIVRGAGLGAVFLPVMASAYIGLSSQQIPHASSTTRIMQQIGGAFGASV 431 DT+ L AL++RG+GLG +F+ +MASA IGL Q+PHAS TRI Q IGGAFG+++ Sbjct: 362 TDTNMILLAVALLIRGSGLGGLFIAIMASALIGLQRDQVPHASIATRIFQTIGGAFGSAI 421 Query: 432 IAIILQNEYNSVMSQNPAAIA 452 +A + + + + + AIA Sbjct: 422 LATVAEQQMAGHVQSDLHAIA 442 >A5N8E7.1 A5N8E7_CLOK5 Predicted transporter protein Length = 472 Score = 342 bits (876), Expect = 6e-92, Method: Compositional matrix adjust. Identities = 185/441 (41%), Positives = 264/441 (59%), Gaps = 5/441 (1%) Query: 12 KQSSETFEAVEQQAAAKKMMWILMVGMLAPLFDTTITNVAIDTLVREFSTSVSTIQWVMT 71 K + E + V +AA +L+VG LAPLFD+T+ NVAI T+ + T++ST+QWV T Sbjct: 7 KPAKEKLDPVVLRAAI-----VLVVGALAPLFDSTMVNVAIHTIAVDMKTTISTVQWVAT 61 Query: 72 SYLLALGMVIPXXXXXXXXXGGKPMWLFSLGMFLLGSVLCSLAWNVESLIFFRTIQGIGG 131 Y+LA+G+ +P G K ++FSL +F +GSV L+WN+ S+I FR IQGI Sbjct: 62 GYVLAMGLTVPISGWATKRFGCKRSYIFSLALFFIGSVCSMLSWNIGSMIIFRVIQGISA 121 Query: 132 GLLMPIMQTLGVRAFKGENMGKMMATXXXXXXXXXXXXXXXXXXXXNHLSWRWIFLVNIP 191 GL+MP++QT V+ G N+G++M+ N L+WR IFLVNIP Sbjct: 122 GLMMPVLQTELVQISGGRNLGRIMSIVSIPAMLGPILGPVLGGIIVNSLNWRAIFLVNIP 181 Query: 192 ICIVAIIMAWRGLPNEERGTRTLRLDLPGLMLLSPAIVLIIYGLGEVSSNHGFGHSXXXX 251 ICIVAI +A G+P ++ + LD+ GL+LLSPA L+IYG+ +++++ G S Sbjct: 182 ICIVAIPLALWGIPADKLLEKKASLDVIGLLLLSPAFALLIYGIAQIATHRGLNSSAVYV 241 Query: 252 XXXXXXXXXXXXXXXXFRKGKEALLDIRLFRVRSLAVSSILLFLSGLTTYGAMLLLPLYF 311 +E L++RLF+ + + S+ILL SG+ T GA+L+LPLY+ Sbjct: 242 PLIIGIVLMASYVVYALNTKREPALNLRLFKSVNFSASNILLIFSGIITNGALLMLPLYY 301 Query: 312 QQVRGESVLISGLLLVPQGIGMLLTRSLAGKLTDQIGSRPVVXXXXXXXXXXXWPFTQLG 371 Q+VRG S L +GL L+PQGIGMLLTRS AGK D+ GSR +V PF G Sbjct: 302 QEVRGASALSAGLWLLPQGIGMLLTRSWAGKAADRDGSRNIVLMSLAAIVMGTLPFAFAG 361 Query: 372 ADTSYYYLGAALIVRGAGLGAVFLPVMASAYIGLSSQQIPHASSTTRIMQQIGGAFGASV 431 DT+ L AL++RG+GLG +F+ +MASA IGL Q+PHAS TRI Q IGGAFG+++ Sbjct: 362 TDTNMILLAVALLIRGSGLGGLFIAIMASALIGLQRDQVPHASIATRIFQTIGGAFGSAI 421 Query: 432 IAIILQNEYNSVMSQNPAAIA 452 +A + + + + + AIA Sbjct: 422 LATVAEQQMAGHVQSDLHAIA 442 >Q9RJX6.1 Q9RJX6_STRCO Putative export protein Length = 496 Score = 314 bits (804), Expect = 1e-83, Method: Compositional matrix adjust. Identities = 175/444 (39%), Positives = 250/444 (56%), Gaps = 4/444 (0%) Query: 11 QKQSSETFEAVEQQAAAKKMMWILMVGMLAPLFDTTITNVAIDTLVREFSTSVSTIQWVM 70 +S A +++ L+VG L +FDTT+ +VA+D L + F ++T+QWV Sbjct: 5 HGAASAAARPDRVDPAVRRIGATLVVGALGVVFDTTVVSVALDDLAKAFDAPLATVQWVS 64 Query: 71 TSYLLALGMVIPXXXXXXXXXGGKPMWLFSLGMFLLGSVLCSLAWNVESLIFFRTIQGIG 130 T YLLA+ + IP GG+ +W+ +LG FLLGSVL +LAWN SLI FR +QG+ Sbjct: 65 TGYLLAVFVTIPLAVWAQARFGGRRLWIAALGGFLLGSVLSALAWNATSLIVFRVVQGLA 124 Query: 131 GGLLMPIMQTLGVRAFKGENMGKMMATXXXXXXXXXXXXXXXXXXXXNHLSWRWIFLVNI 190 GG++MP+M TL ++A +G + G++MA + WRW+F VNI Sbjct: 125 GGIMMPLMATLLMQAARGSHPGRVMAIITVPTALGPILGPVLGGLVLHLADWRWMFFVNI 184 Query: 191 PICIVAIIMAWRGLPNE--ERGTRTLRLDLPGLMLLSPAIVLIIYGLGEVSSNHGFGHSX 248 P C+V +A R LP++ E G RLD+ GL+LL P + ++YGL V G + Sbjct: 185 PFCVVGGWLALRNLPDDRPEPGGPRGRLDVVGLLLLCPGVAALVYGLSRVEGGAGTTGAA 244 Query: 249 XXXXXXXXXXXXXXXXXXXFRKGKEALLDIRLFRVRSLAVSSILLFLSGLTTYGAMLLLP 308 + AL+D+RLFR R++A S+ LLFL G+ YG M+LLP Sbjct: 245 VLVPLLGGLALVGGFTARSLTRPGGALIDLRLFRHRAVASSAALLFLGGIALYGPMMLLP 304 Query: 309 LYFQQVRGESVLISGLLLVPQGIGMLLTRSLAGKLTDQIGSRPVVXXXXXXXXXXXWPFT 368 LYFQQVRGE L +GL L+PQG+G LL R+LAG+ D++G R V PF Sbjct: 305 LYFQQVRGEDALGAGLALIPQGVGALLARALAGRYMDRVGPRRVAVTAFALVAVATVPFA 364 Query: 369 QLGADTSYYYLGAALIVRGAGLGAVFLPVMASAYIGLSSQQIPHASSTTRIMQQIGGAFG 428 + A T L AAL VRG LGA + M +AY+GL Q+IP A TR+ QQIGG+ G Sbjct: 365 FVTAGTGGAVLMAALFVRGIALGAAMIAPMGAAYVGLRHQEIPDAGVITRVAQQIGGSVG 424 Query: 429 ASVIAIILQNEYNSVMSQNPAAIA 452 +++A++LQ + + + P+A+A Sbjct: 425 IALLAVVLQ--HAAAGAHGPSALA 446 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.326 0.139 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,134,684,635 Number of extensions: 75361149 Number of successful extensions: 328191 Number of sequences better than 1.0e-05: 7138 Number of HSP's gapped: 315949 Number of HSP's successfully gapped: 7915 Length of query: 458 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 319 Effective length of database: 1,751,175,118 Effective search space: 558624862642 Effective search space used: 558624862642 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 133 (55.8 bits)