BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0560.0035.p (474 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C3DF85.1 C3DF85_BACTS Spore germination protein KA 582 e-164 A9VER5.1 A9VER5_BACWK GerA spore germination protein 581 e-164 C2SFE4.1 C2SFE4_BACCE Spore germination protein KA 581 e-164 C2ZJQ8.1 C2ZJQ8_BACCE Spore germination protein KA 580 e-164 C2Z356.1 C2Z356_BACCE Spore germination protein KA 580 e-164 >C3DF85.1 C3DF85_BACTS Spore germination protein KA Length = 503 Score = 582 bits (1499), Expect = e-164, Method: Compositional matrix adjust. Identities = 273/466 (58%), Positives = 352/466 (75%) Query: 9 FPSLDQNSDYIEAAMFHTNDLKKRSISFQGRKGMVFYLESITDTELIERNVIVPFSRNPD 68 FPSL +N +YIE + H++D+KK I FQ KG + Y+ES+ D LI + + P D Sbjct: 20 FPSLPENINYIENKLCHSDDIKKLDIPFQNGKGTILYIESLADPNLIHQLALEPLLTRSD 79 Query: 69 KDLTELFTTLSFSMETELNQGIQGLLEGSCLYFSEGISEFYVLFTPAKYERSISEPENEG 128 L + F+TL+ ET LN G+Q LL+G LYF E + F + T +R I+EP+NEG Sbjct: 80 LSLDKAFSTLNMKKETNLNYGVQLLLQGKSLYFHEHVDSFCIFETALSLKRDITEPDNEG 139 Query: 129 VIRGPHNGFIEQMKVNLYLIRKQIASPNLKVRYFTLGKTVPKQVSLVYMENTASPELVNI 188 V+RGPHNGF+E + NL IRK I SPNL V+YFT+G+ + +V++ YM+N A+ +LV Sbjct: 140 VVRGPHNGFVEDLATNLTSIRKLIKSPNLVVKYFTIGEEMHTKVAIAYMQNIANDDLVTE 199 Query: 189 VETRIQKISLDWVLSTGFIQELTEDSPFSIFPQLINTERPDHTATYLLDGYVAILLDGDP 248 V+ R++ I D ++S G+IQE ED+ FS FPQ +NTERPD A L++G VAIL DGDP Sbjct: 200 VKRRLETIKTDALMSPGYIQEFIEDTSFSPFPQQLNTERPDRVAANLMEGRVAILSDGDP 259 Query: 249 TALIVPASFFSFYQTPDDYNNRWFIASFVRFIRLIGFVTAFQLPALYIATVSFHSNVLPL 308 TALIVP + F+FYQ+PDDYNNRW + SFVR IRL+ F+ AF LPA YIATV+FH +VLPL Sbjct: 260 TALIVPVTLFAFYQSPDDYNNRWIVGSFVRMIRLVSFLIAFLLPAFYIATVAFHPDVLPL 319 Query: 309 QLFYTIQGSLTRVPFPPLIEAMLLELIFELLREAGLRLPSRVGQTIGIVGGLVIGDAIVK 368 +L YTI+ SL +VP PP+ EA+L+ELIFELLREAG+RLPSRVGQTIGIVGGLVIGDAIVK Sbjct: 320 ELVYTIKASLEKVPLPPIFEALLMELIFELLREAGIRLPSRVGQTIGIVGGLVIGDAIVK 379 Query: 369 AGLVSYTMIIVVALTAISSFLIPSNEMSSAIRFMRFPLMIAASLFGYIGISFGLTLIFIH 428 AGLVSYTMIIVVALTAISSFL+PSN+MSSA+R +RFPLM+ A++FGY+GISFGL + F+H Sbjct: 380 AGLVSYTMIIVVALTAISSFLVPSNDMSSAVRILRFPLMLLAAIFGYVGISFGLIITFVH 439 Query: 429 LCKLESFGQPYLRPLSPLKFQGLKDTFVRFPIWSLRKSTSNPKTDR 474 LC+L SF PYL PL+P++ + +KD+FVR PIWS + +PK + Sbjct: 440 LCQLHSFHTPYLSPLAPMRIKDMKDSFVRLPIWSFWERPHDPKPKK 485 >A9VER5.1 A9VER5_BACWK GerA spore germination protein Length = 500 Score = 581 bits (1497), Expect = e-164, Method: Compositional matrix adjust. Identities = 271/472 (57%), Positives = 356/472 (75%) Query: 3 NPTQTSFPSLDQNSDYIEAAMFHTNDLKKRSISFQGRKGMVFYLESITDTELIERNVIVP 62 N T+ FPSL +N +YIE + H++D+KK + FQ KG + Y+ES+ D LI + + P Sbjct: 11 NKTKHMFPSLSENVNYIENKLCHSDDIKKLDVPFQNGKGTILYIESLADPNLIHQLALEP 70 Query: 63 FSRNPDKDLTELFTTLSFSMETELNQGIQGLLEGSCLYFSEGISEFYVLFTPAKYERSIS 122 D L + F+TL+ ET LN G+Q LL+G LYF E + F + T +R I+ Sbjct: 71 LLTRSDLSLEKAFSTLNMKKETNLNYGVQLLLQGKSLYFHEHVESFCIFETALSLKRDIT 130 Query: 123 EPENEGVIRGPHNGFIEQMKVNLYLIRKQIASPNLKVRYFTLGKTVPKQVSLVYMENTAS 182 EP+NEGV+RGPH+GF+E + NL +RK I SPNL V+YF LG+ + ++++VYM+N A+ Sbjct: 131 EPDNEGVVRGPHSGFVEDLVTNLASVRKLIKSPNLVVKYFILGEEMHTKIAIVYMQNIAN 190 Query: 183 PELVNIVETRIQKISLDWVLSTGFIQELTEDSPFSIFPQLINTERPDHTATYLLDGYVAI 242 +LV V+ R++ I D ++ G+IQE ED+ FS FPQ +NTERPD TA L++G VAI Sbjct: 191 DDLVAEVKRRLEMIKTDALMPPGYIQEFIEDTSFSPFPQQLNTERPDRTAANLMEGRVAI 250 Query: 243 LLDGDPTALIVPASFFSFYQTPDDYNNRWFIASFVRFIRLIGFVTAFQLPALYIATVSFH 302 L DGDPTALIVP + F+FYQ+PDDYNNRW + SFVR IRL+ F+ AF LPA+YIATV+FH Sbjct: 251 LSDGDPTALIVPVTLFAFYQSPDDYNNRWIVGSFVRMIRLVSFLIAFLLPAIYIATVAFH 310 Query: 303 SNVLPLQLFYTIQGSLTRVPFPPLIEAMLLELIFELLREAGLRLPSRVGQTIGIVGGLVI 362 +VLPL+L YTI+ SL +VP PP+ EA+L+ELIFELLREAG+RLPSRVGQTIGIVGGLVI Sbjct: 311 PDVLPLELVYTIKASLEKVPLPPIFEALLMELIFELLREAGIRLPSRVGQTIGIVGGLVI 370 Query: 363 GDAIVKAGLVSYTMIIVVALTAISSFLIPSNEMSSAIRFMRFPLMIAASLFGYIGISFGL 422 GDAIVKAGLVSYTMIIVVALTAISSFL+PSN+MSSA+R +RFPLM+ A++FGY+GISFGL Sbjct: 371 GDAIVKAGLVSYTMIIVVALTAISSFLVPSNDMSSAVRILRFPLMLLAAIFGYVGISFGL 430 Query: 423 TLIFIHLCKLESFGQPYLRPLSPLKFQGLKDTFVRFPIWSLRKSTSNPKTDR 474 + F+HLC+L SF PYL P++P++ + +KD+FVR PIWS + +PK + Sbjct: 431 IITFVHLCQLHSFHTPYLSPVAPMRIKDMKDSFVRLPIWSFWERPHDPKPKK 482 >C2SFE4.1 C2SFE4_BACCE Spore germination protein KA Length = 500 Score = 581 bits (1497), Expect = e-164, Method: Compositional matrix adjust. Identities = 271/472 (57%), Positives = 356/472 (75%) Query: 3 NPTQTSFPSLDQNSDYIEAAMFHTNDLKKRSISFQGRKGMVFYLESITDTELIERNVIVP 62 N T+ FPSL +N +YIE + H++D+KK + FQ KG + Y+ES+ D LI + + P Sbjct: 11 NKTKHMFPSLSENVNYIENKLCHSDDIKKLDVPFQNGKGTILYIESLADPNLIHQLALEP 70 Query: 63 FSRNPDKDLTELFTTLSFSMETELNQGIQGLLEGSCLYFSEGISEFYVLFTPAKYERSIS 122 D L + F+TL+ ET LN G+Q LL+G LYF E + F + T +R I+ Sbjct: 71 LLTRSDLSLEKAFSTLNMKKETNLNYGVQLLLQGKSLYFHEHVESFCIFETALSLKRDIT 130 Query: 123 EPENEGVIRGPHNGFIEQMKVNLYLIRKQIASPNLKVRYFTLGKTVPKQVSLVYMENTAS 182 EP+NEGV+RGPH+GF+E + NL +RK I SPNL V+YF LG+ + ++++VYM+N A+ Sbjct: 131 EPDNEGVVRGPHSGFVEDLVTNLASVRKLIKSPNLVVKYFILGEEMHTKIAIVYMQNIAN 190 Query: 183 PELVNIVETRIQKISLDWVLSTGFIQELTEDSPFSIFPQLINTERPDHTATYLLDGYVAI 242 +LV V+ R++ I D ++ G+IQE ED+ FS FPQ +NTERPD TA L++G VAI Sbjct: 191 DDLVAEVKRRLEMIKTDALMPPGYIQEFIEDTSFSPFPQQLNTERPDRTAANLMEGRVAI 250 Query: 243 LLDGDPTALIVPASFFSFYQTPDDYNNRWFIASFVRFIRLIGFVTAFQLPALYIATVSFH 302 L DGDPTALIVP + F+FYQ+PDDYNNRW + SFVR IRL+ F+ AF LPA+YIATV+FH Sbjct: 251 LSDGDPTALIVPVTLFAFYQSPDDYNNRWIVGSFVRMIRLVSFLIAFLLPAIYIATVAFH 310 Query: 303 SNVLPLQLFYTIQGSLTRVPFPPLIEAMLLELIFELLREAGLRLPSRVGQTIGIVGGLVI 362 +VLPL+L YTI+ SL +VP PP+ EA+L+ELIFELLREAG+RLPSRVGQTIGIVGGLVI Sbjct: 311 PDVLPLELVYTIKASLEKVPLPPIFEALLMELIFELLREAGIRLPSRVGQTIGIVGGLVI 370 Query: 363 GDAIVKAGLVSYTMIIVVALTAISSFLIPSNEMSSAIRFMRFPLMIAASLFGYIGISFGL 422 GDAIVKAGLVSYTMIIVVALTAISSFL+PSN+MSSA+R +RFPLM+ A++FGY+GISFGL Sbjct: 371 GDAIVKAGLVSYTMIIVVALTAISSFLVPSNDMSSAVRILRFPLMLLAAIFGYVGISFGL 430 Query: 423 TLIFIHLCKLESFGQPYLRPLSPLKFQGLKDTFVRFPIWSLRKSTSNPKTDR 474 + F+HLC+L SF PYL P++P++ + +KD+FVR PIWS + +PK + Sbjct: 431 IITFVHLCQLHSFHTPYLSPVAPMRIKDMKDSFVRLPIWSFWERPHDPKPKK 482 >C2ZJQ8.1 C2ZJQ8_BACCE Spore germination protein KA Length = 501 Score = 580 bits (1496), Expect = e-164, Method: Compositional matrix adjust. Identities = 272/472 (57%), Positives = 356/472 (75%) Query: 3 NPTQTSFPSLDQNSDYIEAAMFHTNDLKKRSISFQGRKGMVFYLESITDTELIERNVIVP 62 N T+ FPSL +N +YIE + H++D+KK + FQ KG++ Y+ES+ D LI + + P Sbjct: 12 NKTKHMFPSLPENINYIENKLCHSDDIKKLDVPFQNGKGIIIYIESLADPNLIHQLALEP 71 Query: 63 FSRNPDKDLTELFTTLSFSMETELNQGIQGLLEGSCLYFSEGISEFYVLFTPAKYERSIS 122 + L + F+TL+ ET LN GIQ LL+G LYF E + F + T +R I Sbjct: 72 LLTRSELSLDKAFSTLNMKKETNLNYGIQLLLQGKSLYFHEHVESFCIFETALSLKRDIK 131 Query: 123 EPENEGVIRGPHNGFIEQMKVNLYLIRKQIASPNLKVRYFTLGKTVPKQVSLVYMENTAS 182 EP+NEGV+RGPH+GF+E + NL IRK I SPN+ V+YFTLG+ + +V++ YM+N A+ Sbjct: 132 EPDNEGVVRGPHSGFVEDLATNLASIRKLIKSPNVVVKYFTLGEEMHTKVAIAYMQNIAN 191 Query: 183 PELVNIVETRIQKISLDWVLSTGFIQELTEDSPFSIFPQLINTERPDHTATYLLDGYVAI 242 +LV V+ R++ I D ++ G+IQE ED+ FS FPQ +NTERPD TA L++G VAI Sbjct: 192 DDLVTEVKRRLETIKTDALMPPGYIQEFIEDTSFSPFPQQLNTERPDRTAANLMEGRVAI 251 Query: 243 LLDGDPTALIVPASFFSFYQTPDDYNNRWFIASFVRFIRLIGFVTAFQLPALYIATVSFH 302 L DGDPTALIVP + F+FYQ+PDDYNNRW + SFVR IRL+ F+ AF LPA+YIATV+FH Sbjct: 252 LSDGDPTALIVPVTLFAFYQSPDDYNNRWIVGSFVRMIRLVSFLIAFLLPAIYIATVAFH 311 Query: 303 SNVLPLQLFYTIQGSLTRVPFPPLIEAMLLELIFELLREAGLRLPSRVGQTIGIVGGLVI 362 +VLPL+L YTI+ SL +VP PP+ EA+L+ELIFELLREAG+RLPSRVGQTIGIVGGLVI Sbjct: 312 PDVLPLELVYTIKASLEKVPLPPIFEALLMELIFELLREAGIRLPSRVGQTIGIVGGLVI 371 Query: 363 GDAIVKAGLVSYTMIIVVALTAISSFLIPSNEMSSAIRFMRFPLMIAASLFGYIGISFGL 422 GDAIVKAGLVSYTMIIVVALTAI+SFL+PSN+MSSA+R +RFPLMI A++FGY+GISFGL Sbjct: 372 GDAIVKAGLVSYTMIIVVALTAIASFLVPSNDMSSAVRILRFPLMILAAIFGYVGISFGL 431 Query: 423 TLIFIHLCKLESFGQPYLRPLSPLKFQGLKDTFVRFPIWSLRKSTSNPKTDR 474 + F+HLC+L SF PYL P++P++ + +KD+FVR PIWS + +PK + Sbjct: 432 IITFVHLCQLHSFHTPYLSPIAPMRIKDMKDSFVRLPIWSFWERPHDPKPKK 483 >C2Z356.1 C2Z356_BACCE Spore germination protein KA Length = 501 Score = 580 bits (1496), Expect = e-164, Method: Compositional matrix adjust. Identities = 272/472 (57%), Positives = 356/472 (75%) Query: 3 NPTQTSFPSLDQNSDYIEAAMFHTNDLKKRSISFQGRKGMVFYLESITDTELIERNVIVP 62 N T+ FPSL +N +YIE + H++D+KK + FQ KG++ Y+ES+ D LI + + P Sbjct: 12 NKTKHMFPSLPENINYIENKLCHSDDIKKLDVPFQNGKGIIIYIESLADPNLIHQLALEP 71 Query: 63 FSRNPDKDLTELFTTLSFSMETELNQGIQGLLEGSCLYFSEGISEFYVLFTPAKYERSIS 122 + L + F+TL+ ET LN GIQ LL+G LYF E + F + T +R I Sbjct: 72 LLTRSELSLDKAFSTLNMKKETNLNYGIQLLLQGKSLYFHEHVESFCIFETALSLKRDIK 131 Query: 123 EPENEGVIRGPHNGFIEQMKVNLYLIRKQIASPNLKVRYFTLGKTVPKQVSLVYMENTAS 182 EP+NEGV+RGPH+GF+E + NL IRK I SPN+ V+YFTLG+ + +V++ YM+N A+ Sbjct: 132 EPDNEGVVRGPHSGFVEDLATNLASIRKLIKSPNVVVKYFTLGEEMHTKVAIAYMQNIAN 191 Query: 183 PELVNIVETRIQKISLDWVLSTGFIQELTEDSPFSIFPQLINTERPDHTATYLLDGYVAI 242 +LV V+ R++ I D ++ G+IQE ED+ FS FPQ +NTERPD TA L++G VAI Sbjct: 192 DDLVTEVKRRLETIKTDALMPPGYIQEFIEDTSFSPFPQQLNTERPDRTAANLMEGRVAI 251 Query: 243 LLDGDPTALIVPASFFSFYQTPDDYNNRWFIASFVRFIRLIGFVTAFQLPALYIATVSFH 302 L DGDPTALIVP + F+FYQ+PDDYNNRW + SFVR IRL+ F+ AF LPA+YIATV+FH Sbjct: 252 LSDGDPTALIVPVTLFAFYQSPDDYNNRWIVGSFVRMIRLVSFLIAFLLPAIYIATVAFH 311 Query: 303 SNVLPLQLFYTIQGSLTRVPFPPLIEAMLLELIFELLREAGLRLPSRVGQTIGIVGGLVI 362 +VLPL+L YTI+ SL +VP PP+ EA+L+ELIFELLREAG+RLPSRVGQTIGIVGGLVI Sbjct: 312 PDVLPLELVYTIKASLEKVPLPPIFEALLMELIFELLREAGIRLPSRVGQTIGIVGGLVI 371 Query: 363 GDAIVKAGLVSYTMIIVVALTAISSFLIPSNEMSSAIRFMRFPLMIAASLFGYIGISFGL 422 GDAIVKAGLVSYTMIIVVALTAI+SFL+PSN+MSSA+R +RFPLMI A++FGY+GISFGL Sbjct: 372 GDAIVKAGLVSYTMIIVVALTAIASFLVPSNDMSSAVRILRFPLMILAAIFGYVGISFGL 431 Query: 423 TLIFIHLCKLESFGQPYLRPLSPLKFQGLKDTFVRFPIWSLRKSTSNPKTDR 474 + F+HLC+L SF PYL P++P++ + +KD+FVR PIWS + +PK + Sbjct: 432 IITFVHLCQLHSFHTPYLSPIAPMRIKDMKDSFVRLPIWSFWERPHDPKPKK 483 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.324 0.140 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,743,177,494 Number of extensions: 114518004 Number of successful extensions: 276274 Number of sequences better than 1.0e-05: 1092 Number of HSP's gapped: 275345 Number of HSP's successfully gapped: 1093 Length of query: 474 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 334 Effective length of database: 1,741,754,103 Effective search space: 581745870402 Effective search space used: 581745870402 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 133 (55.8 bits)