BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0560.0034.p (358 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A9VER4.1 A9VER4_BACWK Spore germination protein 237 1e-60 B7IX11.1 B7IX11_BACC2 Spore germination protein GerKB 237 1e-60 Q3EY38.1 Q3EY38_BACTI Spore germination protein KB 237 1e-60 C3IES8.1 C3IES8_BACTU Spore germination protein KB 234 9e-60 C1EXG6.1 C1EXG6_BACC3 Spore germination protein GerKB 233 2e-59 >A9VER4.1 A9VER4_BACWK Spore germination protein Length = 368 Score = 237 bits (605), Expect = 1e-60, Method: Compositional matrix adjust. Identities = 137/354 (38%), Positives = 194/354 (54%) Query: 2 ITKGQLFLFIIQAQIGVGILSLPSKLNETAKXXXXXXXXXXXXXTQXXXXXXXXXXXXXX 61 I+ Q F+IQ QIGVGILSLP++L+ AK Q Sbjct: 8 ISLAQFTFFVIQCQIGVGILSLPNRLHPIAKGGGWISALIAGLAIQLIILLMWLFLKRFP 67 Query: 62 XXXXXXICXXXXXXXXXXXXXXVYIGYFILLGSNIMLNAVEVLRRWMLQTTPRWAVLALF 121 Y+ YF L+G +MLNA V++ W+LQ TP +A+L LF Sbjct: 68 DADMYESVCMLFGNKFGKLLGFAYVFYFTLIGMTVMLNACNVIKVWILQATPWYAILLLF 127 Query: 122 SIMTLYLAREKLTVLARFYTLASVLFIPLFLFICYGLTQAHLENMFPLLEKGVLNIIKGA 181 +I Y+A V+ RFY +AS+L P+ L I GLT+A +FPL E G NII+G+ Sbjct: 128 TITCCYIAYNTFKVIVRFYVMASILIFPMALLIALGLTRADFSYIFPLTEAGWWNIIQGS 187 Query: 182 KETTISMYGFELMLIIYPLSEGTDKQKLITVSLSNLFVTLFYFFVVSTCLMFFNSEQIKM 241 KET +MYGFE++LI +P G+ KL +S++N FVTLFY F V C + F+ +Q+++ Sbjct: 188 KETITAMYGFEIILIAFPKVNGSPVAKLKAISIANGFVTLFYTFTVWICFIVFSPKQVEL 247 Query: 242 IPEPVIYLMKSLSFFIVDRADILFLPIWTVTLVCSIVSYCYAASMGFSVLFRRKSHRNFT 301 IPEPV YL++SL I+DR D+LF+PIW +T+V SI SY AAS+G +F +H+ Sbjct: 248 IPEPVAYLLRSLHIGIIDRTDLLFIPIWMITVVASIASYYCAASIGIGHIFNLANHKKAV 307 Query: 302 PFVKIITFIIALAPATSVGIHLLDTASTYAAYLIIGGLTPVMLIISLFIKSKGS 355 P V II F +AL T + ++ T + Y+ I L + L+ S+ KG Sbjct: 308 PIVGIIAFSVALFIDTPEELKVIATFTDKFTYIFIVVLPLLFLLYSVIRNKKGE 361 >B7IX11.1 B7IX11_BACC2 Spore germination protein GerKB Length = 368 Score = 237 bits (605), Expect = 1e-60, Method: Compositional matrix adjust. Identities = 139/353 (39%), Positives = 195/353 (55%) Query: 6 QLFLFIIQAQIGVGILSLPSKLNETAKXXXXXXXXXXXXXTQXXXXXXXXXXXXXXXXXX 65 QL F+IQ QIGVGILSLP++L+ AK TQ Sbjct: 12 QLTFFVIQCQIGVGILSLPNRLHPIAKGGGWISVLIAGLATQLIILLMWFLLRRFPDANM 71 Query: 66 XXICXXXXXXXXXXXXXXVYIGYFILLGSNIMLNAVEVLRRWMLQTTPRWAVLALFSIMT 125 I Y+ YF L+G +MLNA V++ W+LQ TP +A+L LF+I Sbjct: 72 YEIACMMFGNKFGKLLGFSYVFYFTLIGMTVMLNACTVIKIWILQATPWYAILLLFTIAC 131 Query: 126 LYLAREKLTVLARFYTLASVLFIPLFLFICYGLTQAHLENMFPLLEKGVLNIIKGAKETT 185 Y+A V+ RFY +AS+L +P+ L I GL++A +FP+ E G NIIK +KET Sbjct: 132 CYVAYNTFKVIVRFYVMASILILPMALLIGLGLSRADFSYIFPITEAGWWNIIKASKETI 191 Query: 186 ISMYGFELMLIIYPLSEGTDKQKLITVSLSNLFVTLFYFFVVSTCLMFFNSEQIKMIPEP 245 +MYGFE+ LI +P GT KL +S++N FVTLFY F V C + F+ +Q+++IPEP Sbjct: 192 TAMYGFEIFLIAFPKVNGTAVAKLKAISIANGFVTLFYAFTVWICFIVFSPKQVELIPEP 251 Query: 246 VIYLMKSLSFFIVDRADILFLPIWTVTLVCSIVSYCYAASMGFSVLFRRKSHRNFTPFVK 305 V YL++SL IVDR D++F+PIW +T+V SI S AAS+G +F +H+ P V Sbjct: 252 VAYLLRSLHIGIVDRTDLIFIPIWMITVVASIASCYAAASIGIGHIFNLANHKKSVPIVG 311 Query: 306 IITFIIALAPATSVGIHLLDTASTYAAYLIIGGLTPVMLIISLFIKSKGSGLA 358 +I+F IAL T + L+ + Y+ I L + L+ SL KG+ A Sbjct: 312 VISFSIALFIDTPEELKLIANFTDKFTYIFIIVLPFLFLLYSLLRNKKGAPYA 364 >Q3EY38.1 Q3EY38_BACTI Spore germination protein KB Length = 371 Score = 237 bits (604), Expect = 1e-60, Method: Compositional matrix adjust. Identities = 139/353 (39%), Positives = 195/353 (55%) Query: 6 QLFLFIIQAQIGVGILSLPSKLNETAKXXXXXXXXXXXXXTQXXXXXXXXXXXXXXXXXX 65 QL F+IQ QIGVGILSLP++L+ AK TQ Sbjct: 15 QLTFFVIQCQIGVGILSLPNRLHPIAKGGGWISVLIAGLATQLIILLMWFLLRRFPDANM 74 Query: 66 XXICXXXXXXXXXXXXXXVYIGYFILLGSNIMLNAVEVLRRWMLQTTPRWAVLALFSIMT 125 I Y+ YF L+G +MLNA V++ W+LQ TP +A+L LF+I Sbjct: 75 YEIACMMFGNKFGKLLGFSYVFYFTLIGMTVMLNACTVIKIWILQATPWYAILLLFTIAC 134 Query: 126 LYLAREKLTVLARFYTLASVLFIPLFLFICYGLTQAHLENMFPLLEKGVLNIIKGAKETT 185 Y+A V+ RFY +AS+L +P+ L I GL++A +FP+ E G NIIK +KET Sbjct: 135 CYVAYNTFKVIVRFYVMASILILPMALLIGLGLSRADFSYIFPITEAGWWNIIKASKETI 194 Query: 186 ISMYGFELMLIIYPLSEGTDKQKLITVSLSNLFVTLFYFFVVSTCLMFFNSEQIKMIPEP 245 +MYGFE+ LI +P GT KL +S++N FVTLFY F V C + F+ +Q+++IPEP Sbjct: 195 TAMYGFEIFLIAFPKVNGTAVAKLKAISIANGFVTLFYAFTVWICFIVFSPKQVELIPEP 254 Query: 246 VIYLMKSLSFFIVDRADILFLPIWTVTLVCSIVSYCYAASMGFSVLFRRKSHRNFTPFVK 305 V YL++SL IVDR D++F+PIW +T+V SI S AAS+G +F +H+ P V Sbjct: 255 VAYLLRSLHIGIVDRTDLIFIPIWMITVVASIASCYAAASIGIGHIFNLANHKKSVPIVG 314 Query: 306 IITFIIALAPATSVGIHLLDTASTYAAYLIIGGLTPVMLIISLFIKSKGSGLA 358 +I+F IAL T + L+ + Y+ I L + L+ SL KG+ A Sbjct: 315 VISFSIALFIDTPEELKLIANFTDKFTYIFIIVLPFLFLLYSLLRNKKGAPYA 367 >C3IES8.1 C3IES8_BACTU Spore germination protein KB Length = 356 Score = 234 bits (597), Expect = 9e-60, Method: Compositional matrix adjust. Identities = 137/349 (39%), Positives = 193/349 (55%) Query: 10 FIIQAQIGVGILSLPSKLNETAKXXXXXXXXXXXXXTQXXXXXXXXXXXXXXXXXXXXIC 69 F+IQ QIGVGILSLP++L+ AK TQ I Sbjct: 4 FVIQCQIGVGILSLPNRLHPIAKGGGWISVLIAGLATQLIILLMWFLLRRFPDANMYEIA 63 Query: 70 XXXXXXXXXXXXXXVYIGYFILLGSNIMLNAVEVLRRWMLQTTPRWAVLALFSIMTLYLA 129 Y+ YF L+G +MLNA V++ W+LQ TP +A+L LF+I Y+A Sbjct: 64 CMMFGNKFGKLLGFSYVFYFTLIGMTVMLNACTVIKIWILQATPWYAILLLFTIACCYVA 123 Query: 130 REKLTVLARFYTLASVLFIPLFLFICYGLTQAHLENMFPLLEKGVLNIIKGAKETTISMY 189 V+ RFY +AS+L +P+ L I GL++A +FP+ E G NIIK +KET +MY Sbjct: 124 YNTFKVIVRFYVMASILILPMALLIGLGLSRADFSYIFPITEAGWWNIIKASKETITAMY 183 Query: 190 GFELMLIIYPLSEGTDKQKLITVSLSNLFVTLFYFFVVSTCLMFFNSEQIKMIPEPVIYL 249 GFE+ LI +P GT KL +S++N FVTLFY F V C + F+ +Q+++IPEPV YL Sbjct: 184 GFEIFLIAFPKVNGTAVAKLKAISIANGFVTLFYAFTVWICFIVFSPKQVELIPEPVAYL 243 Query: 250 MKSLSFFIVDRADILFLPIWTVTLVCSIVSYCYAASMGFSVLFRRKSHRNFTPFVKIITF 309 ++SL IVDR D++F+PIW +T+V SI S AAS+G +F +H+ P V +I+F Sbjct: 244 LRSLHIGIVDRTDLIFIPIWMITVVASIASCYAAASIGIGHIFNLANHKKSVPIVGVISF 303 Query: 310 IIALAPATSVGIHLLDTASTYAAYLIIGGLTPVMLIISLFIKSKGSGLA 358 IAL T + L+ + Y+ I L + L+ SL KG+ A Sbjct: 304 SIALFIDTPEELKLIANFTDKFTYIFIIVLPFLFLLYSLLRNKKGAPYA 352 >C1EXG6.1 C1EXG6_BACC3 Spore germination protein GerKB Length = 368 Score = 233 bits (595), Expect = 2e-59, Method: Compositional matrix adjust. Identities = 134/350 (38%), Positives = 191/350 (54%) Query: 6 QLFLFIIQAQIGVGILSLPSKLNETAKXXXXXXXXXXXXXTQXXXXXXXXXXXXXXXXXX 65 Q F+IQ QIGVGILSLP++L+ AK Q Sbjct: 12 QFTFFVIQCQIGVGILSLPNRLHPIAKGGGWISALIAGLAIQLIILLMWLFLKRFPDADM 71 Query: 66 XXICXXXXXXXXXXXXXXVYIGYFILLGSNIMLNAVEVLRRWMLQTTPRWAVLALFSIMT 125 Y+ YFIL+G +MLN V++ W+LQ TP +A+L LF+I Sbjct: 72 YESVCMLFGNKFGKLLGFAYVFYFILIGMTVMLNVCNVIKVWILQATPWYAILMLFTIAC 131 Query: 126 LYLAREKLTVLARFYTLASVLFIPLFLFICYGLTQAHLENMFPLLEKGVLNIIKGAKETT 185 Y+A V+ RFY + S+L +P+ L I GL++A +FP+ E G NII+ +KET Sbjct: 132 CYVAYNTFKVIVRFYVMTSILILPMALLIALGLSRADFSYIFPITEAGWWNIIQASKETI 191 Query: 186 ISMYGFELMLIIYPLSEGTDKQKLITVSLSNLFVTLFYFFVVSTCLMFFNSEQIKMIPEP 245 +MYGFE+ LI +P G+ KL +S++N FVTLFY F V C + F+ +QI++IPEP Sbjct: 192 TAMYGFEIFLIAFPKVNGSSVAKLKAISIANGFVTLFYAFTVWICFIVFSPKQIELIPEP 251 Query: 246 VIYLMKSLSFFIVDRADILFLPIWTVTLVCSIVSYCYAASMGFSVLFRRKSHRNFTPFVK 305 V YL++SL I+DR D+LF+PIW +T+V SI SY AAS+G +F +H+ P V Sbjct: 252 VAYLLRSLHIGIIDRTDLLFIPIWMITVVASIASYYCAASIGIRHIFNLANHKKAVPVVG 311 Query: 306 IITFIIALAPATSVGIHLLDTASTYAAYLIIGGLTPVMLIISLFIKSKGS 355 II F +AL T + ++ T + Y+ I L + L+ S+ K KG Sbjct: 312 IIAFSVALFIDTPEELKVISTFTDKFTYIFIVVLPLLFLLYSVIRKKKGE 361 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.332 0.144 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,611,908,044 Number of extensions: 58533136 Number of successful extensions: 193612 Number of sequences better than 1.0e-05: 689 Number of HSP's gapped: 192772 Number of HSP's successfully gapped: 689 Length of query: 358 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 221 Effective length of database: 1,770,017,148 Effective search space: 391173789708 Effective search space used: 391173789708 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 132 (55.5 bits)