BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0560.0031.p (316 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KEW2.1 B3KEW2_9BACI ABC transporter related 387 e-106 B1DAH6.1 B1DAH6_9BACL ABC transporter related 380 e-103 B2A6Q3.1 B2A6Q3_NATTJ ABC transporter related 321 6e-86 B5GL44.1 B5GL44_STRCL ATP-binding protein involved in mithramyci... 214 6e-54 Q08QA0.1 Q08QA0_STIAU ATP-binding protein involved in mithramyci... 212 5e-53 >B3KEW2.1 B3KEW2_9BACI ABC transporter related Length = 318 Score = 387 bits (995), Expect = e-106, Method: Compositional matrix adjust. Identities = 196/318 (61%), Positives = 240/318 (75%), Gaps = 5/318 (1%) Query: 1 MDSWIYEVECVNMVYKKGKIKANEDISFLIQRGEILGVLGPNGAGKSTLIKQLVGHLKPT 60 M IY++E VN YKKGK+ AN++ISF +Q+GEILG+LGPNGAGKSTLIKQ+VGHL PT Sbjct: 1 MMKLIYQLEHVNKTYKKGKVVANQNISFDVQQGEILGLLGPNGAGKSTLIKQMVGHLAPT 60 Query: 61 EGQVLYNGLDVYKQTRVVASEVSYYAQEPHALSALTAMEALVFTGQLRGMQKKPXXXXXX 120 G V Y G V QT+ VA EV+YY+QEPHAL++L EAL FTG+LRG+ + Sbjct: 61 SGTVRYKGTSVQTQTKRVAQEVAYYSQEPHALTSLKTWEALYFTGRLRGLTTESALKQTE 120 Query: 121 XXXXXFEMTELRNKSLKRLSGGQKRMVGIGTALIGHSPVLILDEPTNELDPTKRRLVWDL 180 F M +LR+K LK +SGGQKR++GIGT LIG SPV+ILDEPTNELDP KRRLVWDL Sbjct: 121 ELLERFGMQDLRHKLLKNVSGGQKRLIGIGTCLIGFSPVMILDEPTNELDPKKRRLVWDL 180 Query: 181 IQEHNRNGATVILVTHNVLEAEHVVDRVAVINHGKLLAIDSVAKLKQRVDQRLKFEIQTA 240 IQE NR G TVILVTHNVLEAE VVDRVAV+NHGKLLAID V+ LKQRVDQR+K EI T+ Sbjct: 181 IQERNRQGVTVILVTHNVLEAEQVVDRVAVVNHGKLLAIDHVSVLKQRVDQRMKLEITTS 240 Query: 241 FGQREEVEKELAEWGILQRSGENRICLLVDKAQASSVLDRIISHRGLQIEEYAVLPPSLE 300 FG+ + V + L+ G ++GENRI L++K AS +D ++ + L IEEY+++PP+LE Sbjct: 241 FGESDYVCRSLSALGQWTKTGENRIRTLIEKQNASYAID-CLTGQQLPIEEYSLVPPNLE 299 Query: 301 DVYFHI----DQAAKEVV 314 DVYFHI D+ AKE V Sbjct: 300 DVYFHIDDDQDKPAKEEV 317 >B1DAH6.1 B1DAH6_9BACL ABC transporter related Length = 326 Score = 380 bits (975), Expect = e-103, Method: Compositional matrix adjust. Identities = 191/311 (61%), Positives = 231/311 (74%) Query: 5 IYEVECVNMVYKKGKIKANEDISFLIQRGEILGVLGPNGAGKSTLIKQLVGHLKPTEGQV 64 + E++ VN VYKKGK +AN DISF I+ GEILG+LGPNGAGKSTLIKQ+VG L PT G+V Sbjct: 3 LIEIDQVNKVYKKGKKQANIDISFSIREGEILGLLGPNGAGKSTLIKQIVGLLAPTSGEV 62 Query: 65 LYNGLDVYKQTRVVASEVSYYAQEPHALSALTAMEALVFTGQLRGMQKKPXXXXXXXXXX 124 Y G++V KQ + VASE YYAQEPHAL ALTA EAL++TG LRGM KK Sbjct: 63 RYAGINVLKQAKKVASEFGYYAQEPHALGALTAKEALLYTGVLRGMPKKEAEKQTMELLE 122 Query: 125 XFEMTELRNKSLKRLSGGQKRMVGIGTALIGHSPVLILDEPTNELDPTKRRLVWDLIQEH 184 F M E K LK+LSGGQKRMVGIGT +IG S VL+LDEPTNELDP RRLVWD+I E Sbjct: 123 RFGMAEFAGKPLKKLSGGQKRMVGIGTTIIGDSRVLVLDEPTNELDPLNRRLVWDIIVER 182 Query: 185 NRNGATVILVTHNVLEAEHVVDRVAVINHGKLLAIDSVAKLKQRVDQRLKFEIQTAFGQR 244 NR GAT++LVTHNVLEAE VVDRVA IN+G+LL IDSV LKQRVD RLK E+ G+ Sbjct: 183 NRQGATILLVTHNVLEAERVVDRVAFINYGRLLEIDSVPHLKQRVDGRLKLELTAVEGEA 242 Query: 245 EEVEKELAEWGILQRSGENRICLLVDKAQASSVLDRIISHRGLQIEEYAVLPPSLEDVYF 304 + K L EWG +Q++GENR+ LL+DK +A ++LD ++ L I+EYA+LPP+LEDVY Sbjct: 243 GNLMKRLEEWGNIQQTGENRLRLLIDKNRAGTMLDWMVHSNDLPIDEYAMLPPNLEDVYL 302 Query: 305 HIDQAAKEVVG 315 +ID ++ G Sbjct: 303 YIDSQKEKAAG 313 >B2A6Q3.1 B2A6Q3_NATTJ ABC transporter related Length = 321 Score = 321 bits (822), Expect = 6e-86, Method: Compositional matrix adjust. Identities = 153/309 (49%), Positives = 221/309 (71%), Gaps = 3/309 (0%) Query: 1 MDSWIYEVECVNMVYKKG--KIKANEDISFLIQRGEILGVLGPNGAGKSTLIKQLVGHLK 58 M ++E++ V +YKK ++ AN DI+ I+ GEI+G+LGPNGAGKSTL+KQ++ H++ Sbjct: 1 MQDSLFEIDNVTKIYKKKDKQVTANLDINCSIRSGEIIGLLGPNGAGKSTLVKQMLAHIQ 60 Query: 59 PTEGQVLYNGLDVYKQTRVVASEVSYYAQEPHALSALTAMEALVFTGQLRGMQKKPXXXX 118 PT+G++ Y G +V K + +A E+SYY+QEP L+ L EA+ FTG+LRG++ +P Sbjct: 61 PTKGEIRYRGQNVLKNSHKLAQEISYYSQEPSCLTPLQVREAIYFTGRLRGLKHEPANKK 120 Query: 119 XXXXXXXFEMTELRNKSLKRLSGGQKRMVGIGTALIGHSPVLILDEPTNELDPTKRRLVW 178 E+T++++ +K +SGGQ+R+ G+G LIG VLILDEPTNELDP KR+LVW Sbjct: 121 TEELISELELTDVKDNKIKEISGGQRRLTGMGITLIGRPSVLILDEPTNELDPEKRKLVW 180 Query: 179 DLIQEHNRNGATVILVTHNVLEAEHVVDRVAVINHGKLLAIDSVAKLKQRVDQRLKFEIQ 238 +I+E N+ TVILVTHN+LEAE VVDRVAVIN+GKL ID+V KLKQRVDQRLK ++ Sbjct: 181 KIIREKNKQDTTVILVTHNILEAEQVVDRVAVINNGKLQDIDTVDKLKQRVDQRLKCQVS 240 Query: 239 TAFGQREEVEKELAEWGILQRSGENRICLLVDKAQASSVLDRIISH-RGLQIEEYAVLPP 297 T G + + + L WG + + E ++ LL+DK +ASS++ + ++ + L IE ++++PP Sbjct: 241 TESGANDSIGELLDSWGTIISNTETKVDLLIDKEKASSLVKFLTNNEQDLPIENFSIIPP 300 Query: 298 SLEDVYFHI 306 SLEDVYF I Sbjct: 301 SLEDVYFQI 309 >B5GL44.1 B5GL44_STRCL ATP-binding protein involved in mithramycin resistance Length = 313 Score = 214 bits (546), Expect = 6e-54, Method: Compositional matrix adjust. Identities = 117/302 (38%), Positives = 175/302 (57%), Gaps = 12/302 (3%) Query: 4 WIYEVECVNMVYKKGKIKANEDISFLIQRGEILGVLGPNGAGKSTLIKQLVGHLKPTEGQ 63 + YEVE ++ Y AN +SF I+ GE G+LG NGAGK+TLIKQ++G ++P G Sbjct: 18 YAYEVEDLHKRYGDSTEPANAGLSFRIEEGEFFGLLGSNGAGKTTLIKQMIGLVRPDRGS 77 Query: 64 VLYNGLDVYKQTRVVASEVSYYAQEPHALSALTAMEALVFTGQLRGMQKKPXXXXXXXXX 123 + G DV+ +R + V Y Q AL++LT EA+ F+ LRGM + Sbjct: 78 IRLFGHDVFAHSRYTTTHVGYMPQTAFALNSLTVREAVYFSAHLRGMSIRAAKRERDTLL 137 Query: 124 XXFEMTELRNKSLKRLSGGQKRMVGIGTALIGHSPVLILDEPTNELDPTKRRLVWDLIQE 183 +T L K +++LSGG++R++ + L G PVLILDEPTNELDP++RR VWDL+++ Sbjct: 138 EALGLTALARKVVRQLSGGERRLLQLAVTLTGAPPVLILDEPTNELDPSRRRQVWDLLRD 197 Query: 184 -HNRNGATVILVTHNVLEAEHVVDRVAVINHGKLLAIDSVAKLKQRVDQRLKFEIQTAFG 242 H R+G TVIL+THN +EAE V++RV ++ G+L+A+ + A+LK + R++ I G Sbjct: 198 LHQRDGRTVILITHNAIEAEQVIERVGIMREGELVALGAPAELKSSLRSRVRLTITLGEG 257 Query: 243 QREEVEKELAEWGILQRSGENRICLLVDKAQASSVLDRIISHRGL-QIEEYAVLPPSLED 301 L ++ +Q R+ LVD A L R+ L + E+ + +LED Sbjct: 258 D------VLPDYLRIQSEHRGRVVALVDSAD----LPRLTKDVDLTRFPEFTIHTATLED 307 Query: 302 VY 303 VY Sbjct: 308 VY 309 >Q08QA0.1 Q08QA0_STIAU ATP-binding protein involved in mithramycin resistance Length = 611 Score = 212 bits (539), Expect = 5e-53, Method: Compositional matrix adjust. Identities = 124/299 (41%), Positives = 183/299 (61%), Gaps = 9/299 (3%) Query: 22 ANEDISFLIQRGEILGVLGPNGAGKSTLIKQLVGHLKPTEGQVLYNGLDVYKQTRVVASE 81 AN+ +SF I RGEI G+LGPNGAGK+TL+ QL+G ++PT G + +DV + V S Sbjct: 49 ANDQLSFDIHRGEIFGLLGPNGAGKTTLVMQLMGLMRPTSGHIQIEHVDVVRHPDAVKSL 108 Query: 82 VSYYAQEPHALSALTAMEALVFTGQLRGMQKKPXXXXXXXXXXXFEMTELRNKSLKRLSG 141 + + Q AL+ L AL +TG+LRG + + R++ + LSG Sbjct: 109 IGFLPQGELALNYLEVERALHYTGRLRGQSEHDARVQTRQIIEQLGLGSFRSRYINHLSG 168 Query: 142 GQKRMVGIGTALIGHSPVLILDEPTNELDPTKRRLVWDLIQEHNR-NGATVILVTHNVLE 200 G R+VG G AL+G +LILDEPTNELDP KRRLVWD + + NR G T +LVTHNVLE Sbjct: 169 GMARLVGFGMALMGQPRLLILDEPTNELDPHKRRLVWDTLDQLNREQGLTCLLVTHNVLE 228 Query: 201 AEHVVDRVAVINHGKLLAIDSVAKLKQRVDQRLKFEIQTAFGQR---EEVEKELAEWGIL 257 AE V+ R+AV++ G+++A+ + ++KQR ++ E+Q G R E+E+ + ++ Sbjct: 229 AERVIHRLAVMDRGRIIALGTPQEIKQRAGGHVRLELQLKEGLRLEDRELEQLRSHGQVI 288 Query: 258 Q-RSGENRICLLVDKAQASSVLDRIISHRGLQ-IEEYAVLPPSLEDVYFHIDQ-AAKEV 313 + R G R L +A ++SV +I GL+ +E+ + PPSLE+VY +D+ AA+EV Sbjct: 289 EVRGGHYRTYLEPQQAASTSV--ELIQSLGLERVEDLRLAPPSLEEVYLDLDRNAAREV 345 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.135 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,742,537,615 Number of extensions: 68352382 Number of successful extensions: 602584 Number of sequences better than 1.0e-05: 77428 Number of HSP's gapped: 512491 Number of HSP's successfully gapped: 81228 Length of query: 316 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 181 Effective length of database: 1,788,859,178 Effective search space: 323783511218 Effective search space used: 323783511218 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)