BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0560.0018.p (693 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B9DWT3.1 B9DWT3_CLOK1 Putative uncharacterized protein 247 3e-63 A5N336.1 A5N336_CLOK5 Predicted ABC transporter, permease component 247 3e-63 Q24R76.1 Q24R76_DESHY Putative uncharacterized protein 164 4e-38 Q9K9F0.1 Q9K9F0_BACHD ABC transporter (Permease) 105 2e-20 Q97IX1.1 Q97IX1_CLOAB Predicted permease 88 3e-15 >B9DWT3.1 B9DWT3_CLOK1 Putative uncharacterized protein Length = 698 Score = 247 bits (631), Expect = 3e-63, Method: Compositional matrix adjust. Identities = 195/715 (27%), Positives = 340/715 (47%), Gaps = 55/715 (7%) Query: 1 MTFFSIIRKNLLYNAKRYVSIYSVNTLIVTVLFMFGSLLYNSEILRQLGDTTLYDIVRVA 60 MTF+ I+ KNL YN ++S VN+ +V VLF++GSLL+N + R ++ A Sbjct: 1 MTFWHIVGKNLKYNFGHFLSYLFVNSFVVAVLFLYGSLLFNEILARNGSFRAASAYIQAA 60 Query: 61 LIGVIVFSFVFITYSNFSFLKYRGKEFGMYILLGLTSRDLTKMLLLENAGIAGVSLGSGL 120 +++FS VF+ Y+ F+K R +EF +Y+ LG+T RDL +M+ +E+ I S+ G+ Sbjct: 61 AYAILLFSIVFVAYTGIYFVKSRSREFALYLTLGMTDRDLIRMIHIESLAIVAGSMVFGM 120 Query: 121 ITGVVFGKLFYMGLNQIL-----LVNPIRFXXXXXXXXXXXGXXXXXXXXXXXXSMAYLR 175 ++G++ KLFYM L +IL N I + G + ++R Sbjct: 121 LSGLLLSKLFYMILGRILGFTGFSYNDI-YYIDYRTFLLSFGVFILVFFLNMLFTNIFIR 179 Query: 176 RVSVVDALQAAHSREPGKSSIAWGILASVLFAVSAVMLPGIMLE-RWFGGNSAXXXXXXX 234 R+S+V ++++ ++ KS G +A+ S L + W G + Sbjct: 180 RLSIVQMMKSSSTKGVSKSRPVVGAVATAACVFSTFFLHEFLARSEWARGITT------- 232 Query: 235 XXXXCP---------------YVIIGTVIAIMKSAIKRFRKXXXXXXXXXXXVSHRFLSY 279 CP Y +I I I++ K+F +SHRF SY Sbjct: 233 ---NCPVLIPLLTFAVVIVSLYFVIAFGIDIVRYFSKQFPAFYNRNILTFGSLSHRFFSY 289 Query: 280 KTTMYIVTLLIAGALFFSGLTYAMYATTRENNRLDNPYDVMFVENHSINGFHEQEIEQVL 339 K T+YIV+LLI A++F G+ ++Y + PYD M + +IN EI++++ Sbjct: 290 KVTLYIVSLLIFVAIYFMGMGISIYTYNNKTIEEFVPYDFMIETSGNINNISPDEIKELV 349 Query: 340 KDNGIAVEQNQTLEYMEVPEFRNAK-GVWQLWDTH---TTVIGEHQFNQHMGTSYELAED 395 ++ TL++ +RN K G + + + I E +FN+HMG ++ D Sbjct: 350 GGADGVLDTFSTLKFASCQNYRNTKEGFVNYYLENYKDSMFISESEFNRHMGLHIDVKPD 409 Query: 396 QALFVRVHKENMKFEMP---DTIIAFMDP-----EHMNDGMHDYENKESLMQALESYPVK 447 + L V+ + K + P DT+I ++P H + + ++ ++ L + Sbjct: 410 ELLL--VYNSSAKAQEPIDFDTVIT-IEPWREGVTHAEAFGGNSTDMDTFVRKLGNTKHL 466 Query: 448 EYLEGNIREVTEPFANSLHTADVYYGQALVVDDRVYEQIRAAAGESQVKKLHLLKGDIDE 507 Y + + PF +S + A VVDD+VY +++A +++ L LK + Sbjct: 467 VYSGEKTKSMYAPFIDSYGNIEYAGVLADVVDDKVYSELKA---KTETAYLFNLKSGDGK 523 Query: 508 QGFQALLHALQERNGLDASYWNANDQSAMKDADEQRATLEAFRPVYXXXXXXXXXXSSGT 567 + F A+L L++ NG D + W ++ S + A + L RP+Y G Sbjct: 524 KVFNAILDGLRKINGADENLWKSS--SLVFGAKDDITNL---RPIYKQERFDMAFGIGGF 578 Query: 568 TFFIIMFLGALFAVASGAVLYHKVLSDIDQQLENMQSLKRIGVTAKELKQIVSKELGLVF 627 TFF F+G LF ++SG VLY+K+++DID++ E + LKRIG+ +KE ++ ++ L ++F Sbjct: 579 TFFAFSFIGFLFLLSSGIVLYYKIVTDIDEEKEQIVMLKRIGLNSKECRRYLTTHLAILF 638 Query: 628 FLPAVFGLGLGIYYFYFLFSNQPGILQLLGKAGLVALLFVALQIVFYFGSRKKYF 682 F P + G LG Y + P + ++ ++ + L ++ Y RK +F Sbjct: 639 FTPLIIGAILGTVYMHAELVFSPYVDYMMSFILIIIGVVAILDVLLYLTLRKGFF 693 >A5N336.1 A5N336_CLOK5 Predicted ABC transporter, permease component Length = 698 Score = 247 bits (631), Expect = 3e-63, Method: Compositional matrix adjust. Identities = 195/715 (27%), Positives = 340/715 (47%), Gaps = 55/715 (7%) Query: 1 MTFFSIIRKNLLYNAKRYVSIYSVNTLIVTVLFMFGSLLYNSEILRQLGDTTLYDIVRVA 60 MTF+ I+ KNL YN ++S VN+ +V VLF++GSLL+N + R ++ A Sbjct: 1 MTFWHIVGKNLKYNFGHFLSYLFVNSFVVAVLFLYGSLLFNEILARNGSFRAASAYIQAA 60 Query: 61 LIGVIVFSFVFITYSNFSFLKYRGKEFGMYILLGLTSRDLTKMLLLENAGIAGVSLGSGL 120 +++FS VF+ Y+ F+K R +EF +Y+ LG+T RDL +M+ +E+ I S+ G+ Sbjct: 61 AYAILLFSIVFVAYTGIYFVKSRSREFALYLTLGMTDRDLIRMIHIESLAIVAGSMVFGM 120 Query: 121 ITGVVFGKLFYMGLNQIL-----LVNPIRFXXXXXXXXXXXGXXXXXXXXXXXXSMAYLR 175 ++G++ KLFYM L +IL N I + G + ++R Sbjct: 121 LSGLLLSKLFYMILGRILGFTGFSYNDI-YYIDYRTFLLSFGVFILVFFLNMLFTNIFIR 179 Query: 176 RVSVVDALQAAHSREPGKSSIAWGILASVLFAVSAVMLPGIMLE-RWFGGNSAXXXXXXX 234 R+S+V ++++ ++ KS G +A+ S L + W G + Sbjct: 180 RLSIVQMMKSSSTKGVSKSRPVVGAVATAACVFSTFFLHEFLARSEWARGITT------- 232 Query: 235 XXXXCP---------------YVIIGTVIAIMKSAIKRFRKXXXXXXXXXXXVSHRFLSY 279 CP Y +I I I++ K+F +SHRF SY Sbjct: 233 ---NCPVLIPLLTFAVVIVSLYFVIAFGIDIVRYFSKQFPAFYNRNILTFGSLSHRFFSY 289 Query: 280 KTTMYIVTLLIAGALFFSGLTYAMYATTRENNRLDNPYDVMFVENHSINGFHEQEIEQVL 339 K T+YIV+LLI A++F G+ ++Y + PYD M + +IN EI++++ Sbjct: 290 KVTLYIVSLLIFVAIYFMGMGISIYTYNNKTIEEFVPYDFMIETSGNINNISPDEIKELV 349 Query: 340 KDNGIAVEQNQTLEYMEVPEFRNAK-GVWQLWDTH---TTVIGEHQFNQHMGTSYELAED 395 ++ TL++ +RN K G + + + I E +FN+HMG ++ D Sbjct: 350 GGADGVLDTFSTLKFASCQNYRNTKEGFVNYYLENYKDSMFISESEFNRHMGLHIDVKPD 409 Query: 396 QALFVRVHKENMKFEMP---DTIIAFMDP-----EHMNDGMHDYENKESLMQALESYPVK 447 + L V+ + K + P DT+I ++P H + + ++ ++ L + Sbjct: 410 ELLL--VYNSSAKAQEPIDFDTVIT-IEPWREGVTHAEAFGGNSTDMDTFVRKLGNTKHL 466 Query: 448 EYLEGNIREVTEPFANSLHTADVYYGQALVVDDRVYEQIRAAAGESQVKKLHLLKGDIDE 507 Y + + PF +S + A VVDD+VY +++A +++ L LK + Sbjct: 467 VYSGEKTKSMYAPFIDSYGNIEYAGVLADVVDDKVYSELKA---KTETAYLFNLKSGDGK 523 Query: 508 QGFQALLHALQERNGLDASYWNANDQSAMKDADEQRATLEAFRPVYXXXXXXXXXXSSGT 567 + F A+L L++ NG D + W ++ S + A + L RP+Y G Sbjct: 524 KVFNAILDGLRKINGADENLWKSS--SLVFGAKDDITNL---RPIYKQERFDMAFGIGGF 578 Query: 568 TFFIIMFLGALFAVASGAVLYHKVLSDIDQQLENMQSLKRIGVTAKELKQIVSKELGLVF 627 TFF F+G LF ++SG VLY+K+++DID++ E + LKRIG+ +KE ++ ++ L ++F Sbjct: 579 TFFAFSFIGFLFLLSSGIVLYYKIVTDIDEEKEQIVMLKRIGLNSKECRRYLTTHLAILF 638 Query: 628 FLPAVFGLGLGIYYFYFLFSNQPGILQLLGKAGLVALLFVALQIVFYFGSRKKYF 682 F P + G LG Y + P + ++ ++ + L ++ Y RK +F Sbjct: 639 FTPLIIGAILGTVYMHAELVFSPYVDYMMSFILIIIGVVAILDVLLYLTLRKGFF 693 >Q24R76.1 Q24R76_DESHY Putative uncharacterized protein Length = 535 Score = 164 bits (414), Expect = 4e-38, Method: Compositional matrix adjust. Identities = 135/526 (25%), Positives = 240/526 (45%), Gaps = 27/526 (5%) Query: 1 MTFFSIIRKNLLYNAKRYVSIYSVNTLIVTVLFMFGSLLYNSEILRQLGDTTLYDIVRVA 60 MTF I+ KNL YN R++S VN+ +V VLF++GSLL+N + + ++ A Sbjct: 1 MTFRHIVGKNLKYNLGRFLSYLFVNSFVVAVLFLYGSLLFNETLAQNGSFRAASAYIQAA 60 Query: 61 LIGVIVFSFVFITYSNFSFLKYRGKEFGMYILLGLTSRDLTKMLLLENAGIAGVSLGSGL 120 +++F+ VF+TY+ F+K R +EF +Y+ LG+T RDL +M+ +E+ I S+ G+ Sbjct: 61 AYAILLFAIVFVTYTGIYFVKSRSREFALYLTLGMTDRDLIRMIHMESLVIVAGSMVMGV 120 Query: 121 ITGVVFGKLFYMGLNQILLVNPIRFXXXXXXXXXXXGXXXXXXXXXXXXSM----AYLRR 176 ++G++ +LFYM L +IL + + G +M ++RR Sbjct: 121 LSGLLLSRLFYMILGRILGFTGVSYSDIYYIDYRTFGLSLGVFILVFLFNMLFTNIFIRR 180 Query: 177 VSVVDALQAAHSREPGKSSIAWGILASVLFAVSAVMLPGIMLER-WFGGNSA-----XXX 230 +S+ ++++ ++ KS G +A+V S L + R W ++ Sbjct: 181 LSIAQLMKSSSTKGIAKSRPVIGAIAAVACIFSTFFLHEFLARREWARAMTSHDPVLIPL 240 Query: 231 XXXXXXXXCPYVIIGTVIAIMKSAIKRFRKXXXXXXXXXXXVSHRFLSYKTTMYIVTLLI 290 Y +I I I++ KRF + HRF SYK T+YIV+LLI Sbjct: 241 LTFAVVIVSLYFVIAFGIDILRFFSKRFPGFYNRHILTLGSLGHRFFSYKVTLYIVSLLI 300 Query: 291 AGALFFSGLTYAMYATTRENNRLDNPYDVMFVENHSINGFHEQEIEQVLKDNGIAVEQNQ 350 A++F G+ ++Y + PYD M +N ++I+ +++ +G + Sbjct: 301 FVAIYFMGMGTSIYTYNDKTLEEFVPYDFMIETRGDLNKISPEKIKGIVEGSGGVLGTFS 360 Query: 351 TLEYMEVPEFRNA----KGVWQLWDTHTTVIGEHQFNQHMGTSYELAEDQALFVRVHKEN 406 LE+ +RN+ + + + + VI E +FN+HMG ++ D+ L V + Sbjct: 361 PLEFAGCQNYRNSVDGFRNDYLEYHRDSMVISESEFNRHMGLQLDVQPDELLLVYNSSAH 420 Query: 407 MKFEMP-DTIIAFMDPEHMNDGMHDYE-------NKESLMQALESYPVKEYLEGNIREVT 458 + + DT+I E +G+ E + ++ + L Y ++ Sbjct: 421 AQESITYDTVITV---EPWREGVTQAEAFGANPTDMDTFIGTLGERKHLVYDRDKTSKMH 477 Query: 459 EPFANSLHTADVYYGQALVVDDRVYEQIRAAAGESQVKKLHLLKGD 504 PF +S + A V+DD+VY ++ A V +L GD Sbjct: 478 APFIDSYGNIEYAGVLANVIDDKVYGELEAET--ETVYLFNLKSGD 521 >Q9K9F0.1 Q9K9F0_BACHD ABC transporter (Permease) Length = 522 Score = 105 bits (261), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 91/407 (22%), Positives = 181/407 (44%), Gaps = 11/407 (2%) Query: 1 MTFFSIIRKNLLYNAKRYVSIYSVNTLIVTVLFMFGSLLYNSEIL--RQLGDTTLYDIVR 58 MTF ++ K N ++Y+ + N+ V ++F+F +L +N +L QL + + + Sbjct: 1 MTFTQLVWKMAKKNKRKYLFYFIANSFSVALIFIFCTLAFNDRLLNSEQLAEG-VEQFLI 59 Query: 59 VALIGVIVFSFVFITYSNFSFLKYRGKEFGMYILLGLTSRDLTKMLLLENAGIAGVSLGS 118 + + + +F FI S+ F+K R KEFG+++ LG++ +D+ K++LLENAGIA +SL Sbjct: 60 IPTVALALFCLFFINLSHGIFMKQRKKEFGLFLSLGMSYQDMVKLMLLENAGIAFLSLVV 119 Query: 119 GLITGVVFGKLFYMGLNQILLVNPIRFXXXXXXXXXXXGXXXXXXXXXXXXSMAYLRRVS 178 GL++G VF +LF++ + V I F ++ R Sbjct: 120 GLLSGTVFSRLFFLTSMYYIEVTDISFQLQGTFYLYAIAIFSVIFILAIGKTLFITRGQK 179 Query: 179 VVDALQAAHSREPGKSSIAWGILASVLFAVSAVMLPGIMLERWFGGNSAXXXXXXXXXXX 238 +V ++ E K S G + +L S V L + ++++ + Sbjct: 180 IVAIMKENRLSEQQKGSPWLGGIGGILVVASLVFLYVMAIDQYPDMDGYFLLGSTVVLFI 239 Query: 239 CPYVIIGTVIAIMKSAIKRFRKXXXXXXXXXXXVSHRFLSYKTTMYIVTLLIAGALFFSG 298 Y+ + + + + +KR ++++F + +++ ++ + +S Sbjct: 240 GLYLALSQLGSFLIQMVKRNPSIYYRNLLHLSNLNYKFKQLTSIFFLLIVMTMVTILYS- 298 Query: 299 LTYAMYA-TTRENNRLDN-PYDVMFVENHSINGFHEQEIEQVLKDNGIAVEQNQTLEYME 356 ++ +Y+ TT E ++ PYD+ FV+ + N ++I+Q+ + + + +E+ + Sbjct: 299 -SFVLYSVTTAEQQAIERYPYDIGFVQTDTKNNVSLEDIQQIFHTDQHKITTHHQVEFFD 357 Query: 357 VPEFRNAKGVWQLWDTHTTVIGEHQFNQHMGTSYELAEDQALFVRVH 403 G +D T + FNQ S EL E L V H Sbjct: 358 YIMPHPTYG----FDERFTFMPTEAFNQISSQSTELREGTYLLVLNH 400 >Q97IX1.1 Q97IX1_CLOAB Predicted permease Length = 652 Score = 88.2 bits (217), Expect = 3e-15, Method: Compositional matrix adjust. Identities = 50/147 (34%), Positives = 89/147 (60%), Gaps = 6/147 (4%) Query: 1 MTFFSIIRKNLLYNAKRYVSIYSVNTLIVTVLFMFGSLLYNSEI-----LRQLGDTTLYD 55 MT +S++ KN+ N +++ Y N +V V F+F + + N + + Q+G T + Sbjct: 1 MTLYSLVLKNIKGNLNKFIMYYLSNAFVVMVFFIFANFILNPNVKSIKTMGQMGAVTT-E 59 Query: 56 IVRVALIGVIVFSFVFITYSNFSFLKYRGKEFGMYILLGLTSRDLTKMLLLENAGIAGVS 115 I+ I +++F+ VF YS SFLK R KEFG+ + GLT ++ + ++ EN +A +S Sbjct: 60 IMYGCEIVILIFTLVFTNYSTSSFLKSREKEFGLLSMFGLTKSEIRRYVMAENLIVAMLS 119 Query: 116 LGSGLITGVVFGKLFYMGLNQILLVNP 142 + +G++ G++F KLF+M ++ ILL+N Sbjct: 120 ILTGVLLGMLFSKLFFMAVSVILLLNS 146 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.324 0.139 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,537,354,482 Number of extensions: 140276866 Number of successful extensions: 577984 Number of sequences better than 1.0e-05: 1042 Number of HSP's gapped: 577525 Number of HSP's successfully gapped: 1460 Length of query: 693 Length of database: 3,060,696,203 Length adjustment: 143 Effective length of query: 550 Effective length of database: 1,713,491,058 Effective search space: 942420081900 Effective search space used: 942420081900 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 135 (56.6 bits)