BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0557.0099.p (298 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KIR5.1 B3KIR5_9BACI ATP-dependent helicase HrpB 260 1e-67 B1DLS9.1 B1DLS9_9BACL ATP-dependent helicase HrpB 238 7e-61 C0Z909.1 C0Z909_BREBN Putative ATP-dependent helicase 232 3e-59 A1HNU7.1 A1HNU7_9FIRM ATP-dependent helicase HrpB 225 3e-57 B0TA29.1 B0TA29_HELMI ATP-dependent helicase hrpb 224 9e-57 >B3KIR5.1 B3KIR5_9BACI ATP-dependent helicase HrpB Length = 836 Score = 260 bits (664), Expect = 1e-67, Method: Compositional matrix adjust. Identities = 139/253 (54%), Positives = 172/253 (67%), Gaps = 4/253 (1%) Query: 1 MKQLPITQVLPELKTILNTSTAAVLTAEPGAGKTTGTPPAFLDEPWMAGKTILMLEPRRL 60 M +LPI +VLP+LK L+T T AVL AEPGAGKTT P AFL+EPW+AGK I+MLEPRR+ Sbjct: 1 MTELPIHRVLPQLKKALSTGTHAVLIAEPGAGKTTQVPLAFLNEPWLAGKRIIMLEPRRI 60 Query: 61 XXXXXXXXXXXXXXXXXXQTVGYRMRNDTKVGPDTRIVVVTEGVLTRMLQSDPSLGEVGL 120 +T+GYR+R D+K+G DTRI VVTEGVLTRMLQ DP L +G+ Sbjct: 61 AARSAAGYMASSLGEAIGETIGYRVRMDSKIGRDTRIEVVTEGVLTRMLQDDPELTGIGM 120 Query: 121 VIFDEFHERSLHADLGLALTLEAQAVLREDLRILIMSATLDSGRVSAMLXXXXGAPVVNC 180 +IFDEFHER+L AD GLA +E+QAVLREDLR+L+MSATL++ V+ +L APV+ Sbjct: 121 IIFDEFHERNLQADAGLAFAMESQAVLREDLRLLVMSATLEAEPVAKLL---GDAPVIVS 177 Query: 181 PGRTFPVETIYLPGSGDRQLEQAXXXXAVRRALAEQPGDVLVFLPGEREIRRTGRELESG 240 GR FPV T YL ++ + A+ RAL+EQ GD+LVFLPG REIRRT EL Sbjct: 178 EGRAFPVTTRYLRSFPLDEIHKG-AANAITRALSEQEGDILVFLPGAREIRRTANELLRT 236 Query: 241 TLPPGTVLRPLYG 253 L V+R LY Sbjct: 237 GLSAEVVIRELYS 249 >B1DLS9.1 B1DLS9_9BACL ATP-dependent helicase HrpB Length = 830 Score = 238 bits (606), Expect = 7e-61, Method: Compositional matrix adjust. Identities = 135/254 (53%), Positives = 160/254 (62%), Gaps = 4/254 (1%) Query: 1 MKQLPITQVLPELKTILNTSTAAVLTAEPGAGKTTGTPPAFLDEPWMAGKTILMLEPRRL 60 MKQLPI VLPEL L T AVL AEPGAGKTT P A L E W+ G+ I+MLEPRRL Sbjct: 1 MKQLPIDAVLPELLQALRVGTNAVLVAEPGAGKTTRVPLALLQETWLDGRKIVMLEPRRL 60 Query: 61 XXXXXXXXXXXXXXXXXXQTVGYRMRNDTKVGPDTRIVVVTEGVLTRMLQSDPSLGEVGL 120 +TVGYR+R D+KV TRI VVTEGVLTRMLQ D +L VG Sbjct: 61 AARSAAKYMAASLGEQVGETVGYRVRLDSKVSAKTRIEVVTEGVLTRMLQEDQALERVGA 120 Query: 121 VIFDEFHERSLHADLGLALTLEAQAVLREDLRILIMSATLDSGRVSAMLXXXXGAPVVNC 180 V+FDEFHER LH DLGLALTL++Q++LREDLR+LIMSATLD+ V+ +L GAP++ Sbjct: 121 VLFDEFHERHLHGDLGLALTLQSQSLLREDLRLLIMSATLDAEPVAELL---GGAPIIQS 177 Query: 181 PGRTFPVETIYLPGSGDRQLEQAXXXXAVRRALAEQPGDVLVFLPGEREIRRTGRELESG 240 GR +PVETIY + ++E V A+ E GDVLVFLPG EIRRT L Sbjct: 178 SGRAYPVETIYRSTRVEGRIESQAASTIV-EAIRENEGDVLVFLPGVGEIRRTAGHLMQS 236 Query: 241 TLPPGTVLRPLYGQ 254 L + L+G Sbjct: 237 GLASSVRIAELHGN 250 >C0Z909.1 C0Z909_BREBN Putative ATP-dependent helicase Length = 834 Score = 232 bits (592), Expect = 3e-59, Method: Compositional matrix adjust. Identities = 132/254 (51%), Positives = 166/254 (65%), Gaps = 4/254 (1%) Query: 1 MKQLPITQVLPELKTILNTSTAAVLTAEPGAGKTTGTPPAFLDEPWMAGKTILMLEPRRL 60 M LPI +VLPEL L T AVL A PGAGKTT P A +E W+ + I+ML PRRL Sbjct: 1 MNALPINEVLPELVNTLREETNAVLVAAPGAGKTTRVPLALREEAWLHNRRIVMLVPRRL 60 Query: 61 XXXXXXXXXXXXXXXXXXQTVGYRMRNDTKVGPDTRIVVVTEGVLTRMLQSDPSLGEVGL 120 QTVGYR++ +++VGP TRI V+TEG+LTRMLQ DP L +VGL Sbjct: 61 AARQAAAYMAALLGEEVGQTVGYRVKRESRVGPGTRIEVMTEGILTRMLQDDPELSDVGL 120 Query: 121 VIFDEFHERSLHADLGLALTLEAQAVLREDLRILIMSATLDSGRVSAMLXXXXGAPVVNC 180 VIFDEFHER+LHADLGLAL+L+AQ++ REDLRIL+MSATLD+ VSA+L AP+V+ Sbjct: 121 VIFDEFHERNLHADLGLALSLQAQSLFREDLRILVMSATLDAEPVSALL---GNAPIVSS 177 Query: 181 PGRTFPVETIYLPGSGDRQLEQAXXXXAVRRALAEQPGDVLVFLPGEREIRRTGRELESG 240 GR FPVET +L +LE+A + +AL ++ GD+LVFLPG +EI R LE Sbjct: 178 KGRMFPVETRFLTSPMQGRLEEAIVQM-IFQALRQEEGDMLVFLPGAKEIHRVQELLEQS 236 Query: 241 TLPPGTVLRPLYGQ 254 + + PLYG Sbjct: 237 GVGTNIRIAPLYGN 250 >A1HNU7.1 A1HNU7_9FIRM ATP-dependent helicase HrpB Length = 845 Score = 225 bits (574), Expect = 3e-57, Method: Compositional matrix adjust. Identities = 129/251 (51%), Positives = 162/251 (64%), Gaps = 3/251 (1%) Query: 3 QLPITQVLPELKTILNTSTAAVLTAEPGAGKTTGTPPAFLDEPWMAGKTILMLEPRRLXX 62 LPI V+P+LK L T AVL A PG+GKTT P A LDEPW+AG+ +LMLEPRRL Sbjct: 14 HLPIEPVIPDLKEALRRQTNAVLVAPPGSGKTTRIPLALLDEPWLAGQRVLMLEPRRLAA 73 Query: 63 XXXXXXXXXXXXXXXXQTVGYRMRNDTKVGPDTRIVVVTEGVLTRMLQSDPSLGEVGLVI 122 +TVGYR+R DT+VG +TR+ V+TEGVLTR+LQ+DP+L VGLVI Sbjct: 74 RAAARYMAASLGEQVGETVGYRVRLDTRVGANTRVEVITEGVLTRLLQADPALEGVGLVI 133 Query: 123 FDEFHERSLHADLGLALTLEAQAVLREDLRILIMSATLDSGRVSAMLXXXXGAPVVNCPG 182 FDEFHER++HADLGL + L+AQAVLR DLR+L+MSATLD+ V+A+L APV+ G Sbjct: 134 FDEFHERNIHADLGLTMCLQAQAVLRPDLRLLVMSATLDAAPVAALL---GDAPVLTGTG 190 Query: 183 RTFPVETIYLPGSGDRQLEQAXXXXAVRRALAEQPGDVLVFLPGEREIRRTGRELESGTL 242 +PVET YL + + + AL PGD+LVFLPG EIRR + + L Sbjct: 191 AAYPVETRYLDRPVEEGRRETVIVDTIIEALTAAPGDILVFLPGAAEIRRVEAAIAARGL 250 Query: 243 PPGTVLRPLYG 253 + PLYG Sbjct: 251 GANVRVAPLYG 261 >B0TA29.1 B0TA29_HELMI ATP-dependent helicase hrpb Length = 874 Score = 224 bits (570), Expect = 9e-57, Method: Compositional matrix adjust. Identities = 135/254 (53%), Positives = 166/254 (65%), Gaps = 5/254 (1%) Query: 1 MKQLPITQVLPELKTILNTSTAAVLTAEPGAGKTTGTPPAFLDEPWMAGKTILMLEPRRL 60 M LPI +LP LK L T+ VL A PGAGKTT P A L+EPW+AG+ I+MLEPRRL Sbjct: 1 MTSLPIEAILPALKEALRVRTSTVLVAPPGAGKTTRVPLALLNEPWLAGRRIIMLEPRRL 60 Query: 61 XXXXXXXXXXXXXXXXXXQTVGYRMRNDTKVGPDTRIVVVTEGVLTRMLQSDPSLGEVGL 120 +TVG+R+R +T+VGP T I VVTEGVLTR LQ+DP+L +VGL Sbjct: 61 AARSAARHMAVSLGEAVGETVGFRVRLETRVGPSTAIEVVTEGVLTRRLQADPALEDVGL 120 Query: 121 VIFDEFHERSLHADLGLALTLEAQAVLREDLRILIMSATLDSGRVSAMLXXXXGAPVVNC 180 VIFDEFHERSL ADLGLAL L++QA+LREDLRIL+MSATLD+ V+A+L AP++ Sbjct: 121 VIFDEFHERSLAADLGLALCLQSQALLREDLRILVMSATLDAEPVAALL---GDAPIIAS 177 Query: 181 PGRTFPVETIYLPGSGDRQLEQAXXXXAVRRALAEQPGDVLVFLPGEREIRRT-GRELES 239 GR F V+T YL + +E A VR AL GD+LVFLPG +EIRR R +E Sbjct: 178 EGRPFSVDTRYLERRTEGTME-AQVARTVREALLNDEGDILVFLPGMKEIRRVEARLVEM 236 Query: 240 GTLPPGTVLRPLYG 253 G + PL+G Sbjct: 237 GVGTGKVRICPLHG 250 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.139 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,561,774,866 Number of extensions: 59130502 Number of successful extensions: 136062 Number of sequences better than 1.0e-05: 2559 Number of HSP's gapped: 128224 Number of HSP's successfully gapped: 2560 Length of query: 298 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 163 Effective length of database: 1,788,859,178 Effective search space: 291584046014 Effective search space used: 291584046014 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)