BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0557.0093.p (264 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KIR2.1 B3KIR2_9BACI Short-chain dehydrogenase/reductase SDR 324 4e-87 B1DH61.1 B1DH61_9BACL Short-chain dehydrogenase/reductase SDR 252 2e-65 C1P8U9.1 C1P8U9_BACCO Short-chain dehydrogenase/reductase SDR 250 1e-64 B3KIU1.1 B3KIU1_9BACI Short-chain dehydrogenase/reductase SDR 243 9e-63 C4KZH2.1 C4KZH2_EXISA Short-chain dehydrogenase/reductase SDR 234 4e-60 >B3KIR2.1 B3KIR2_9BACI Short-chain dehydrogenase/reductase SDR Length = 265 Score = 324 bits (831), Expect = 4e-87, Method: Compositional matrix adjust. Identities = 158/259 (61%), Positives = 196/259 (75%) Query: 5 RFLGKXXXXXXXXXXXXXXXXXQMAREGANVALFDLVNERTSVLEQKLNKLRKDCALAID 64 RF G+ Q+A+EGANVALFDL+++RT E ++N +R+ A A D Sbjct: 7 RFHGRTAIITGAGSGIGKAAAIQLAKEGANVALFDLLDDRTHQAESEINSIREGAARAFD 66 Query: 65 VDTSDEKRMEEAVRRTVEHFGGLDIVFANAGINGAVGPIEELSLSDWERTMSVNLTGTFL 124 VDT+D R+E+AV TVE FGG+DIVFANAGING + PI+E+ L +W +T++VNL GTFL Sbjct: 67 VDTTDPDRVEKAVLETVEIFGGVDIVFANAGINGVMTPIDEMKLEEWAKTLNVNLNGTFL 126 Query: 125 TLKYSIPHLKEKGKXXXXXXXXXXXXXRFASFGWSPYSTTKAGQVAFAKMAALELAKFKI 184 T+KY+IPHLK++G+ F++FG S YSTTKAGQVAFAKMAALELA+FKI Sbjct: 127 TVKYAIPHLKKRGQGSIIITSSVNGTRTFSNFGMSAYSTTKAGQVAFAKMAALELAQFKI 186 Query: 185 RVNVICPGAISTNIDESTEFNEEVETIVIPIEFPEGAQPLADGPGKPENVADLVSFLASD 244 RVNVICPGAISTNID+ST+ ++E IVIP+E+PEG+QPLADGPG PE VADLV+FLASD Sbjct: 187 RVNVICPGAISTNIDQSTKKQGDLEEIVIPVEYPEGSQPLADGPGTPEEVADLVAFLASD 246 Query: 245 DAIHITGAQIIIDGAESLL 263 + HITGAQI IDGAESLL Sbjct: 247 QSRHITGAQIFIDGAESLL 265 >B1DH61.1 B1DH61_9BACL Short-chain dehydrogenase/reductase SDR Length = 267 Score = 252 bits (644), Expect = 2e-65, Method: Compositional matrix adjust. Identities = 128/237 (54%), Positives = 166/237 (70%), Gaps = 2/237 (0%) Query: 27 QMAREGANVALFDLVNERTSVLEQKLNKLRKDCALAIDVDTSDEKRMEEAVRRTVEHFGG 86 + A EGAN+ L D + SV+ ++ ++A++ D SDE + EA + + FG Sbjct: 31 RFAEEGANLVLLDRDRQTLSVVCREAEAFGAK-SIAVEADISDEHSLAEAYDKAISAFGQ 89 Query: 87 LDIVFANAGINGAVGPIEELSLSDWERTMSVNLTGTFLTLKYSIPHLKEKGKXXXXXXXX 146 LDIVFANAGING + PIE +S +WE T++VNLTGTFLT+K++IP LKEKG Sbjct: 90 LDIVFANAGINGTISPIEHMSKEEWEATINVNLTGTFLTVKHAIPLLKEKGGSIIITSSI 149 Query: 147 XXXXXRFASFGWSPYSTTKAGQVAFAKMAALELAKFKIRVNVICPGAISTNIDESTEFNE 206 F++FG+S YS++KAG+ AF +MAALELA++ IRVN ICPGAISTNID+ST+ Sbjct: 150 NGNRV-FSNFGFSAYSSSKAGETAFTRMAALELAEYGIRVNAICPGAISTNIDQSTKPKP 208 Query: 207 EVETIVIPIEFPEGAQPLADGPGKPENVADLVSFLASDDAIHITGAQIIIDGAESLL 263 E+E I IP+EFPEG PL DGPG+P VA+LV FLASD+A HITG + IDGAESLL Sbjct: 209 ELEEIRIPVEFPEGGHPLEDGPGQPAQVANLVLFLASDEASHITGTSVYIDGAESLL 265 >C1P8U9.1 C1P8U9_BACCO Short-chain dehydrogenase/reductase SDR Length = 260 Score = 250 bits (638), Expect = 1e-64, Method: Compositional matrix adjust. Identities = 124/237 (52%), Positives = 169/237 (71%), Gaps = 2/237 (0%) Query: 27 QMAREGANVALFDLVNERTSVLEQKLNKLRKDCALAIDVDTSDEKRMEEAVRRTVEHFGG 86 ++A EGA V + D+ +R +++++ + + A+A++VD +D ++++ AV +TV + Sbjct: 24 KLAAEGAKVCVMDIEQKRADEVKRRIEQNGGE-AMALEVDVTDPEQVKTAVDKTVREWDR 82 Query: 87 LDIVFANAGINGAVGPIEELSLSDWERTMSVNLTGTFLTLKYSIPHLKEKGKXXXXXXXX 146 LDIVF+NAGING V PIE+L+ DWE+T+S NL GTFL KY+IPH+K +G Sbjct: 83 LDIVFSNAGINGTVAPIEDLTPDDWEKTLSTNLKGTFLLTKYAIPHMKNRGGSIIITSSI 142 Query: 147 XXXXXRFASFGWSPYSTTKAGQVAFAKMAALELAKFKIRVNVICPGAISTNIDESTEFNE 206 F++ G S YS++KAGQVAF KMAALELA++KIRVN +CPGAI T I+++T E Sbjct: 143 NGNRI-FSNIGMSAYSSSKAGQVAFMKMAALELARYKIRVNAVCPGAIETKIEQNTNRTE 201 Query: 207 EVETIVIPIEFPEGAQPLADGPGKPENVADLVSFLASDDAIHITGAQIIIDGAESLL 263 E+E + IPIEFPEG QPL+ PGKPE VADLV FLASDD+ HI+G I IDG ESLL Sbjct: 202 ELEKVQIPIEFPEGDQPLSGRPGKPEEVADLVLFLASDDSSHISGTDIYIDGTESLL 258 >B3KIU1.1 B3KIU1_9BACI Short-chain dehydrogenase/reductase SDR Length = 263 Score = 243 bits (621), Expect = 9e-63, Method: Compositional matrix adjust. Identities = 124/236 (52%), Positives = 161/236 (68%), Gaps = 2/236 (0%) Query: 28 MAREGANVALFDLVNERTSVLEQKLNKLRKDCALAIDVDTSDEKRMEEAVRRTVEHFGGL 87 MA +GA + L D+ ER +++ + + L + D S + +++ + E +G + Sbjct: 29 MAEQGAKLLLIDIDRERVENVKKTIEDGGGEV-LTGNCDISKAESLKDCFSQAAEQWGKI 87 Query: 88 DIVFANAGINGAVGPIEELSLSDWERTMSVNLTGTFLTLKYSIPHLKEKGKXXXXXXXXX 147 DIVFANAGING PIE + L +W+ T++ NL GTF T+KY+IP+LK+ G Sbjct: 88 DIVFANAGINGTKAPIEIMELDEWDATINTNLRGTFATVKYAIPYLKDHGGSIIITSSIN 147 Query: 148 XXXXRFASFGWSPYSTTKAGQVAFAKMAALELAKFKIRVNVICPGAISTNIDESTEFNEE 207 F FG+S YSTTKAGQVAF KMAALELA++KIRVN ICPGAI+TNIDEST E Sbjct: 148 GNRV-FTGFGFSAYSTTKAGQVAFMKMAALELARYKIRVNAICPGAIATNIDESTIAKPE 206 Query: 208 VETIVIPIEFPEGAQPLADGPGKPENVADLVSFLASDDAIHITGAQIIIDGAESLL 263 ++ + IPIE+PEG QPLADGPG+P VA LV+FLASDDA HITG +I IDGAESL+ Sbjct: 207 LDEVTIPIEYPEGDQPLADGPGQPSQVAQLVAFLASDDASHITGTEIYIDGAESLI 262 >C4KZH2.1 C4KZH2_EXISA Short-chain dehydrogenase/reductase SDR Length = 261 Score = 234 bits (598), Expect = 4e-60, Method: Compositional matrix adjust. Identities = 126/235 (53%), Positives = 156/235 (66%), Gaps = 5/235 (2%) Query: 30 REGANVALFDLVNERTSVLEQKLNKLRKDCALAIDVDTSDEKRMEEAVRRTVEHFGGLDI 89 + GA VA+ DL E L + + R A+ I D SDE M+EA RT+E FG LDI Sbjct: 31 QAGAKVAIVDLNEEDAMRLASLVGEER---AIGISADVSDESAMKEAYERTMERFGQLDI 87 Query: 90 VFANAGINGAVGPIEELSLSDWERTMSVNLTGTFLTLKYSIPHLKEKGKXXXXXXXXXXX 149 VFANAG NG + PIE LSL DW + NLTGTFLT+K++IPHLKE G Sbjct: 88 VFANAGRNGTITPIEHLSLDDWNGVVETNLTGTFLTVKHAIPHLKENGG-SIIITSSING 146 Query: 150 XXRFASFGWSPYSTTKAGQVAFAKMAALELAKFKIRVNVICPGAISTNIDESTEFNEE-V 208 F +FG+S Y+TTKAGQ FAKMAA ELA+F IRVN ICPGAI TNID+ST ++ + Sbjct: 147 NRYFKNFGFSGYATTKAGQTGFAKMAAAELAQFGIRVNSICPGAIDTNIDDSTNRDDALL 206 Query: 209 ETIVIPIEFPEGAQPLADGPGKPENVADLVSFLASDDAIHITGAQIIIDGAESLL 263 E IVIPIE+P G QPLA G + VAD+V F AS+ + H+TG+++ +DGAESLL Sbjct: 207 EEIVIPIEYPLGNQPLAGKSGSAKQVADVVLFFASNMSSHVTGSEVYVDGAESLL 261 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.134 0.376 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,259,301,760 Number of extensions: 46722569 Number of successful extensions: 201502 Number of sequences better than 1.0e-05: 17392 Number of HSP's gapped: 181208 Number of HSP's successfully gapped: 17465 Length of query: 264 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 131 Effective length of database: 1,807,701,208 Effective search space: 236808858248 Effective search space used: 236808858248 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 130 (54.7 bits)