BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0557.0059.p (290 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A3DGI2.1 A3DGI2_CLOTH Cysteine synthase EC=2.5.1.47 391 e-107 B4BC06.1 B4BC06_CLOTM Cysteine synthase EC=2.5.1.47 391 e-107 Q24PR4.1 Q24PR4_DESHY Putative uncharacterized protein 380 e-104 A1HQ97.1 A1HQ97_9FIRM Cysteine synthase A 379 e-103 B8G0Z3.1 B8G0Z3_DESHD Cysteine synthase A 379 e-103 >A3DGI2.1 A3DGI2_CLOTH Cysteine synthase EC=2.5.1.47 Length = 311 Score = 391 bits (1004), Expect = e-107, Method: Compositional matrix adjust. Identities = 199/284 (70%), Positives = 228/284 (80%) Query: 1 MPNISKNLTELIGNTPLLELANYGKSHQVEARLIGKLEYFNPAGSVKDRIGYAMIQDAEA 60 M I+KNLTELIGNTPLLEL+NY +++ +EA LI KLEYFNPA SVKDRIGYAMI+DAE Sbjct: 1 MAKIAKNLTELIGNTPLLELSNYNRANNLEAVLIAKLEYFNPASSVKDRIGYAMIKDAEE 60 Query: 61 KGLIHKDSVLIEPTSGNTGIGLXXXXXXLGYRMIIVLPETFSVERRKLLGALGAELVLTP 120 KG+I+KD+V+IEPTSGNTGI L GYR+I+ +PET S+ERR LL ALGAELVLTP Sbjct: 61 KGIINKDTVIIEPTSGNTGIALAFVAAARGYRVILTMPETMSIERRNLLKALGAELVLTP 120 Query: 121 GAEGMLGAVQKAEELAAAIPHSFILQQFSNPANPQIHRDTTAEEIWRDTGGAVDIFXXXX 180 GA+GM GA++KAEELA IP+SFI QQFSNPANP+IHR TTAEEIWRDT G VDIF Sbjct: 121 GADGMGGAIRKAEELAREIPNSFIPQQFSNPANPEIHRRTTAEEIWRDTDGQVDIFVAGV 180 Query: 181 XXXXXXXXXXEVLKARNPEVQVVAVEPADSPVLSGGKPGGHQIQGIGANFVPANFNFGVV 240 EVLK R P+V++VAVEP DSPVLSGG G H+IQGIGA FVP NFN VV Sbjct: 181 GTGGTISGVGEVLKQRKPDVKIVAVEPFDSPVLSGGTKGPHKIQGIGAGFVPDNFNRAVV 240 Query: 241 DEIFQVENRHAFETARSLAATEGLLVGISSGAAVYAASQIAKRP 284 DEIF+V+N AFET+R LA TEGLLVGISSGAA +AA+QIAKRP Sbjct: 241 DEIFKVKNEEAFETSRKLARTEGLLVGISSGAAAFAATQIAKRP 284 >B4BC06.1 B4BC06_CLOTM Cysteine synthase EC=2.5.1.47 Length = 311 Score = 391 bits (1004), Expect = e-107, Method: Compositional matrix adjust. Identities = 199/284 (70%), Positives = 228/284 (80%) Query: 1 MPNISKNLTELIGNTPLLELANYGKSHQVEARLIGKLEYFNPAGSVKDRIGYAMIQDAEA 60 M I+KNLTELIGNTPLLEL+NY +++ +EA LI KLEYFNPA SVKDRIGYAMI+DAE Sbjct: 1 MAKIAKNLTELIGNTPLLELSNYNRANNLEAVLIAKLEYFNPASSVKDRIGYAMIKDAEE 60 Query: 61 KGLIHKDSVLIEPTSGNTGIGLXXXXXXLGYRMIIVLPETFSVERRKLLGALGAELVLTP 120 KG+I+KD+V+IEPTSGNTGI L GYR+I+ +PET S+ERR LL ALGAELVLTP Sbjct: 61 KGIINKDTVIIEPTSGNTGIALAFVAAARGYRVILTMPETMSIERRNLLKALGAELVLTP 120 Query: 121 GAEGMLGAVQKAEELAAAIPHSFILQQFSNPANPQIHRDTTAEEIWRDTGGAVDIFXXXX 180 GA+GM GA++KAEELA IP+SFI QQFSNPANP+IHR TTAEEIWRDT G VDIF Sbjct: 121 GADGMGGAIRKAEELAREIPNSFIPQQFSNPANPEIHRRTTAEEIWRDTDGQVDIFVAGV 180 Query: 181 XXXXXXXXXXEVLKARNPEVQVVAVEPADSPVLSGGKPGGHQIQGIGANFVPANFNFGVV 240 EVLK R P+V++VAVEP DSPVLSGG G H+IQGIGA FVP NFN VV Sbjct: 181 GTGGTISGVGEVLKQRKPDVKIVAVEPFDSPVLSGGTKGPHKIQGIGAGFVPDNFNRAVV 240 Query: 241 DEIFQVENRHAFETARSLAATEGLLVGISSGAAVYAASQIAKRP 284 DEIF+V+N AFET+R LA TEGLLVGISSGAA +AA+QIAKRP Sbjct: 241 DEIFKVKNEEAFETSRKLARTEGLLVGISSGAAAFAATQIAKRP 284 >Q24PR4.1 Q24PR4_DESHY Putative uncharacterized protein Length = 311 Score = 380 bits (977), Expect = e-104, Method: Compositional matrix adjust. Identities = 191/284 (67%), Positives = 224/284 (78%) Query: 1 MPNISKNLTELIGNTPLLELANYGKSHQVEARLIGKLEYFNPAGSVKDRIGYAMIQDAEA 60 M I+++LT+LIGNTPLLEL+NY ++ ++EA++I KLEYFNP SVKDRIGYAMI+DAE Sbjct: 1 MSKIARSLTDLIGNTPLLELSNYNRTTELEAKVIAKLEYFNPLSSVKDRIGYAMIKDAEE 60 Query: 61 KGLIHKDSVLIEPTSGNTGIGLXXXXXXLGYRMIIVLPETFSVERRKLLGALGAELVLTP 120 KGL++KD+V+IEPTSGNTGI L GYR+I+ +PET SVERR LL ALGAELVLTP Sbjct: 61 KGLLNKDTVIIEPTSGNTGIALAFVSAARGYRLILTMPETMSVERRNLLKALGAELVLTP 120 Query: 121 GAEGMLGAVQKAEELAAAIPHSFILQQFSNPANPQIHRDTTAEEIWRDTGGAVDIFXXXX 180 G GM GA+QKAEELAA +SFI QQF+NPANP+IHR TTAEEIWRDT G VDIF Sbjct: 121 GPAGMKGAIQKAEELAAEYQNSFIPQQFNNPANPEIHRQTTAEEIWRDTDGEVDIFVAGV 180 Query: 181 XXXXXXXXXXEVLKARNPEVQVVAVEPADSPVLSGGKPGGHQIQGIGANFVPANFNFGVV 240 E LK + P V+V+AVEPADSPVLSGG PG H+IQGIGA FVP +N V+ Sbjct: 181 GTGGTVTGVGEALKKKKPGVKVIAVEPADSPVLSGGTPGAHKIQGIGAGFVPGVYNSEVI 240 Query: 241 DEIFQVENRHAFETARSLAATEGLLVGISSGAAVYAASQIAKRP 284 DEIFQV+N AFET+R L+ TEGLLVGISSGAA YAA+QIAKRP Sbjct: 241 DEIFQVKNEEAFETSRKLSKTEGLLVGISSGAAAYAATQIAKRP 284 >A1HQ97.1 A1HQ97_9FIRM Cysteine synthase A Length = 312 Score = 379 bits (973), Expect = e-103, Method: Compositional matrix adjust. Identities = 191/284 (67%), Positives = 219/284 (77%) Query: 1 MPNISKNLTELIGNTPLLELANYGKSHQVEARLIGKLEYFNPAGSVKDRIGYAMIQDAEA 60 M I+ NLT+LIGNTPLLEL NY ++ + A+LI KLEYFNP SVKDRIGYAMI+DAE Sbjct: 1 MAKIATNLTDLIGNTPLLELRNYNRAKSLNAKLIAKLEYFNPLSSVKDRIGYAMIKDAED 60 Query: 61 KGLIHKDSVLIEPTSGNTGIGLXXXXXXLGYRMIIVLPETFSVERRKLLGALGAELVLTP 120 KGLI+KDSV+IEPTSGNTGI L GYR+I+ +PET S+ERR LL ALGAELVLTP Sbjct: 61 KGLINKDSVIIEPTSGNTGIALAFVAAAKGYRLILTMPETMSIERRNLLKALGAELVLTP 120 Query: 121 GAEGMLGAVQKAEELAAAIPHSFILQQFSNPANPQIHRDTTAEEIWRDTGGAVDIFXXXX 180 GAEGM GA+++AEELAA IP+SFILQQF NPANP IHR TTAEEIW DT G VDIF Sbjct: 121 GAEGMKGAIRRAEELAAEIPNSFILQQFKNPANPAIHRTTTAEEIWNDTDGQVDIFVGGV 180 Query: 181 XXXXXXXXXXEVLKARNPEVQVVAVEPADSPVLSGGKPGGHQIQGIGANFVPANFNFGVV 240 EV+K R P +VVAVEP DSPVLSGGKPG H+IQGIGA FVP N ++ Sbjct: 181 GTGGTITGVGEVIKQRKPSFKVVAVEPFDSPVLSGGKPGPHKIQGIGAGFVPDVLNVKII 240 Query: 241 DEIFQVENRHAFETARSLAATEGLLVGISSGAAVYAASQIAKRP 284 DEI +V+N AF TAR LA TEGLLVGISSGAA +AA+Q+A+RP Sbjct: 241 DEIIKVKNEEAFATARELAKTEGLLVGISSGAAAFAATQVAQRP 284 >B8G0Z3.1 B8G0Z3_DESHD Cysteine synthase A Length = 311 Score = 379 bits (972), Expect = e-103, Method: Compositional matrix adjust. Identities = 190/284 (66%), Positives = 224/284 (78%) Query: 1 MPNISKNLTELIGNTPLLELANYGKSHQVEARLIGKLEYFNPAGSVKDRIGYAMIQDAEA 60 M I+++LT+LIGNTPLLEL+NY ++ ++EA++I KLEYFNP SVKDRIGYAMI+DAE Sbjct: 1 MSKIARSLTDLIGNTPLLELSNYNRTTELEAKVIAKLEYFNPLSSVKDRIGYAMIKDAEE 60 Query: 61 KGLIHKDSVLIEPTSGNTGIGLXXXXXXLGYRMIIVLPETFSVERRKLLGALGAELVLTP 120 KGL++KD+V+IEPTSGNTGI L GYR+I+ +PET SVERR LL ALGAELVLTP Sbjct: 61 KGLLNKDTVIIEPTSGNTGIALAFVSAARGYRLILTMPETMSVERRNLLKALGAELVLTP 120 Query: 121 GAEGMLGAVQKAEELAAAIPHSFILQQFSNPANPQIHRDTTAEEIWRDTGGAVDIFXXXX 180 G GM GA+QKAEELAA +SFI QQF+NPANP+IHR TTAEEIWRDT G VDIF Sbjct: 121 GPAGMKGAIQKAEELAAEYKNSFIPQQFNNPANPEIHRQTTAEEIWRDTDGEVDIFVAGV 180 Query: 181 XXXXXXXXXXEVLKARNPEVQVVAVEPADSPVLSGGKPGGHQIQGIGANFVPANFNFGVV 240 E LK + P V+V+AVEPADSPVLSGG PG H+IQGIGA FVP ++ V+ Sbjct: 181 GTGGTVTGVGEALKQKKPGVKVIAVEPADSPVLSGGTPGAHKIQGIGAGFVPGVYSSEVI 240 Query: 241 DEIFQVENRHAFETARSLAATEGLLVGISSGAAVYAASQIAKRP 284 DEIFQV+N AFET+R L+ TEGLLVGISSGAA YAA+QIAKRP Sbjct: 241 DEIFQVKNEEAFETSRKLSKTEGLLVGISSGAAAYAATQIAKRP 284 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.135 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,615,168,482 Number of extensions: 64061837 Number of successful extensions: 149517 Number of sequences better than 1.0e-05: 3441 Number of HSP's gapped: 142434 Number of HSP's successfully gapped: 3466 Length of query: 290 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 156 Effective length of database: 1,798,280,193 Effective search space: 280531710108 Effective search space used: 280531710108 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 130 (54.7 bits)