BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0557.0045.p (297 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K6V8.1 B3K6V8_9BACI Aminoglycoside phosphotransferase 309 3e-82 B1DLY4.1 B1DLY4_9BACL Aminoglycoside phosphotransferase 161 6e-38 A8JCM0.1 A8JCM0_CHLRE Predicted protein 86 4e-15 A4RR02.1 A4RR02_OSTLU Predicted protein 79 6e-13 C5BWD3.1 C5BWD3_9MICO Aminoglycoside phosphotransferase 65 6e-09 >B3K6V8.1 B3K6V8_9BACI Aminoglycoside phosphotransferase Length = 333 Score = 309 bits (791), Expect = 3e-82, Method: Compositional matrix adjust. Identities = 154/291 (52%), Positives = 197/291 (67%), Gaps = 1/291 (0%) Query: 1 VLRIYDTHRDRDKIEFEHAVLQKLGNLSLPFKVPVPVSTPAGETLVKLKDADGKFACLFH 60 V+RIY+THRD+ +IEFE AVL L L FKVP PV + +G+ +V+++D ++ACLF Sbjct: 39 VMRIYNTHRDKARIEFEFAVLDSLQRAPLSFKVPTPVPSTSGDKMVRVQDGSARYACLFE 98 Query: 61 YIEGDSPAEEEAGYFESFGETAGFLSAVLADLRPGLIPVYRPYYELRQAYPLCSDAAIQE 120 YIEG P EE SFGE G L LA G+ PVY PYYEL Q+YP CS IQ+ Sbjct: 99 YIEGARPDEESIQATYSFGEKTGELVGALAACSIGMEPVYPPYYELLQSYPSCSRTFIQD 158 Query: 121 LCLNPPRPFSGLAQELKLLYAAYDQIADSLAGLKELPHQLVHGDLNASNLLLETSDHRQV 180 C PP F L++L AY +I+ L LK LP QLVHGDLN SNLL+++ +V Sbjct: 159 FCKRPPVEFQEQGNALQILQEAYTEISGRLESLKNLPQQLVHGDLNFSNLLVDSEQSGKV 218 Query: 181 AALLDFEFCTYDVRAMEPAVILSGLLGQ-EEKTTVRDFCRGFSRRVRLSEAEINAVPVLM 239 ALLDFEFCT DVRAMEPAV++SG LG EEK +R FC GF+ RVRL+E EI A+PVL+ Sbjct: 219 IALLDFEFCTKDVRAMEPAVVISGFLGMAEEKDAIRQFCEGFASRVRLTEEEIAAIPVLL 278 Query: 240 LLRKVDVFLHFVTRYLEGTDEVHVLQEQVKLLSEDVSQLTAQTSAVLEILS 290 LR VDVFLHF++RY EGTDE HVLQEQ+ +L+ + +L + + ++L+ Sbjct: 279 RLRLVDVFLHFLSRYREGTDESHVLQEQIDMLAAGLKRLEQSQAWMEDVLT 329 >B1DLY4.1 B1DLY4_9BACL Aminoglycoside phosphotransferase Length = 333 Score = 161 bits (408), Expect = 6e-38, Method: Compositional matrix adjust. Identities = 104/300 (34%), Positives = 148/300 (49%), Gaps = 24/300 (8%) Query: 1 VLRIYDTHRDRDKIEFEHAVLQKLGNLSLPFKVPVPVSTPAGETLVKLKDADGKFACLFH 60 VLR+Y+ HRD + + EH VL L FKVP+PV +G+T+ + G A L+ Sbjct: 43 VLRVYNNHRDTNIVSLEHHVLFALYRQQPGFKVPIPVENRSGDTITVA--SSGALAALYR 100 Query: 61 YIEGDSPAEEEAGYFESFGETAGFLSAVLADLRPGLIPVYRPYYELRQAYPLCSDAAIQE 120 YI G+ P ++ + S GETA +S L ++ P+Y PYY L + Y S E Sbjct: 101 YIPGERPTVQQKAHIRSLGETAARVSKALRGMKISNKPIYDPYYLLEETY---SSLIHHE 157 Query: 121 L--CLNPPRPFSGLAQELKLLYAAYDQIADSLAGLKELPHQLVHGDLNASNLLLETSDHR 178 L L F A + + ++ +L +KELPHQ +HGDLN SN + E Sbjct: 158 LPAILQSSEQFLSKADKAAAILNQVKDLSGALQAVKELPHQWIHGDLNFSNTVAEGE--- 214 Query: 179 QVAALLDFEFCTYDVRAMEPAVILSGLLG---QEEKTTVRDFCRGFSRRVRLSEAEINAV 235 + +LDFEFCT DVRAME V E +R FC G+ +L+ E+ + Sbjct: 215 FIIGVLDFEFCTVDVRAMELVVAQIDFFKGGDSEIWERLRLFCEGYGSIDKLTPEEVEVL 274 Query: 236 PVLMLLRKVDVFLHFVTRYLEGTDEVHVLQEQVKLLSEDVSQLTAQTSAVLEILSGEQEQ 295 P+L+ LR +DVFLHF RY EG D+ + V+ QT + E + +EQ Sbjct: 275 PLLIKLRMLDVFLHFAGRYNEGLDDAGL-----------VAGFIDQTYQICEWVDRSEEQ 323 >A8JCM0.1 A8JCM0_CHLRE Predicted protein Length = 210 Score = 85.9 bits (211), Expect = 4e-15, Method: Compositional matrix adjust. Identities = 59/170 (34%), Positives = 91/170 (53%), Gaps = 8/170 (4%) Query: 119 QELCLNPPRPFSGLAQELKLLYAAYDQIADSLAG-LKE-LPHQLVHGDLNASNLLLETSD 176 +E+ NP F+ + + L +I LA LKE LP Q++HGDL+ N+++ D Sbjct: 32 KEVATNPQ--FNACRESIDFLVGEIRKIEAKLAEYLKEGLPMQMIHGDLHYDNVMVVGDD 89 Query: 177 HRQVAALLDFEFCTYDVRAMEPAVILSGLLGQEEKT-TVRDFCRGFSRRVRLSEAEINAV 235 V+ LLDFEFC YD RAME AV LS + ++E V F G+ ++ L+E+EI A+ Sbjct: 90 ---VSGLLDFEFCAYDWRAMELAVALSRYVSEDEPLPLVERFVSGYCQKGVLTESEIAAI 146 Query: 236 PVLMLLRKVDVFLHFVTRYLEGTDEVHVLQEQVKLLSEDVSQLTAQTSAV 285 P L+ LR ++F R + G D + L + ++ V + A A+ Sbjct: 147 PDLINLRIFSNAVYFTGRAIAGEDTLESLTSRAGAYAKRVRWVNANRDAL 196 >A4RR02.1 A4RR02_OSTLU Predicted protein Length = 249 Score = 78.6 bits (192), Expect = 6e-13, Method: Compositional matrix adjust. Identities = 44/135 (32%), Positives = 75/135 (55%), Gaps = 2/135 (1%) Query: 156 LPHQLVHGDLNASNLLLETSDHRQVAALLDFEFCTYDVRAMEPAVILSGLLGQEEKTT-V 214 LP L+HGD++ N L + + + V ++DFEF +YD R ME A LS +G+ + V Sbjct: 111 LPETLIHGDVHYDNSLADETTGK-VTGIIDFEFASYDWRMMEAAAGLSKYVGERDPLPFV 169 Query: 215 RDFCRGFSRRVRLSEAEINAVPVLMLLRKVDVFLHFVTRYLEGTDEVHVLQEQVKLLSED 274 D+ +GF RR +EAEI+A+P L+ LR ++ ++FV R D++ L + + + Sbjct: 170 VDYIKGFCRRATPTEAEIDALPDLIKLRVLETVVYFVARSAAKEDDISQLALRAENYAAR 229 Query: 275 VSQLTAQTSAVLEIL 289 V + A + + + Sbjct: 230 VRWIDANAQVIRDTM 244 >C5BWD3.1 C5BWD3_9MICO Aminoglycoside phosphotransferase Length = 335 Score = 65.5 bits (158), Expect = 6e-09, Method: Compositional matrix adjust. Identities = 83/251 (33%), Positives = 118/251 (47%), Gaps = 21/251 (8%) Query: 1 VLRIYDTHRDRDKIEFEHAVLQKLGNLS-LPFKVPVPVSTPAGETLVKLKDADGKFACLF 59 V+R Y T D + + EH +L L + LPF VP P+ T G T V A G+ +F Sbjct: 39 VVRRYSTL-DAEHVAAEHRLLAALDERADLPFAVPAPIRTSDGATFVV---AGGRPVAVF 94 Query: 60 HYIEGDSPAEEEAGYFESFGETAGFLSAVLADLRPGLIPVYRPYYELRQAYPLCSDAAIQ 119 Y +PA + E GE G L A LA+L L P P + +P D + Sbjct: 95 AY-RAGAPAPPDPASMELAGEALGLLDAALAELPAELAPRVGPA-SMAGLHPAVDD--LD 150 Query: 120 ELCLNPPRPFSGLAQELKLLYAAYDQIADSLAGLKE-LPHQLVHGDLNASNLLLETSDHR 178 EL R G + AA+ ++ A L+ LP Q++H DL+ SN+L+ S Sbjct: 151 ELGAELERRLPG-DDGVAWFRAAWAPADEAYASLRRTLPTQILHADLSPSNVLIHQS--- 206 Query: 179 QVAALLDFEFCTYDVRAMEPAVILS-----GLLGQEEKTTVRDFCRGFSRRVRLSEAEIN 233 +V+A+LDF+ +R +P + G G EE T+R F RG+ R + AE Sbjct: 207 RVSAILDFDHACLGLRVEDPVAAMHMAAVFGTAGPEE--TLRAFRRGYVRTGEILPAEDA 264 Query: 234 AVPVLMLLRKV 244 AVPVL+ R V Sbjct: 265 AVPVLLRRRGV 275 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.138 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,747,636,632 Number of extensions: 72791376 Number of successful extensions: 193121 Number of sequences better than 1.0e-05: 8 Number of HSP's gapped: 193133 Number of HSP's successfully gapped: 8 Length of query: 297 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 162 Effective length of database: 1,788,859,178 Effective search space: 289795186836 Effective search space used: 289795186836 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)