BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0557.0041.p (197 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q4H4G3.1 Q4H4G3_BACCI Possible oxidoreductase 253 5e-66 P96674.1 YDEQ_BACSU Uncharacterized NAD(P)H oxidoreductase ydeQ ... 182 1e-44 P54439.2 YRKL_BACSU Uncharacterized NAD(P)H oxidoreductase yrkL ... 180 6e-44 Q8VSR5.1 Q8VSR5_BACAM Nitroreductase 180 6e-44 A7Z9G9.1 A7Z9G9_BACA2 YwrO 177 5e-43 >Q4H4G3.1 Q4H4G3_BACCI Possible oxidoreductase Length = 191 Score = 253 bits (647), Expect = 5e-66, Method: Compositional matrix adjust. Identities = 114/189 (60%), Positives = 151/189 (79%), Gaps = 3/189 (1%) Query: 8 AHPRIEHSRVNKRWLSELRGYPDQVTVHELYKAYPHFTIHAENEQDLVARYDRIVLQFPI 67 AHP ++ SRVNKRW+ EL+ +P +VTVHELY AYP + EQ L+A +R+VLQFP+ Sbjct: 2 AHPNLQQSRVNKRWVEELKKHPGEVTVHELYGAYPEKVLDIGREQALLAECERLVLQFPL 61 Query: 68 QWYSMPALLKQWLDGVFTTSWLYETGGKAVAGKELVLAMSIGGDESTYQSGGMIGFTISE 127 QWYS P LLKQWLD VFTT WL+ GGKAVAGKEL++A+SIGG TY++GG+IG+TISE Sbjct: 62 QWYSTPPLLKQWLDEVFTTGWLFGPGGKAVAGKELLIAVSIGGAAETYEAGGLIGYTISE 121 Query: 128 LIRPVQVFSNQVGMTFLPHYKFYGANKATEDQIDNSAQRYVEHITSPDLNPVVVRKRMLA 187 L RP+Q F+NQ+GMT LPH+KF+GAN+A ++ I++SA+RYV HI +P+L+P + R+RML Sbjct: 122 LTRPLQAFANQIGMTMLPHFKFHGANQAADELIESSAERYVRHILTPELDPRIARRRMLN 181 Query: 188 EM---MKAS 193 +M M+AS Sbjct: 182 DMKQKMQAS 190 >P96674.1 YDEQ_BACSU Uncharacterized NAD(P)H oxidoreductase ydeQ EC=1.6.99.- Length = 197 Score = 182 bits (462), Expect = 1e-44, Method: Compositional matrix adjust. Identities = 92/189 (48%), Positives = 121/189 (64%), Gaps = 1/189 (0%) Query: 1 MSILIVAAHPRIEHSRVNKRWLSELRGYPDQVTVHELYKAYPHFTIHAENEQDLVARYDR 60 M L++ HP IE SR+NK+W + PD VTVH+LY+ Y I E EQ + +DR Sbjct: 4 MKTLVLVVHPNIESSRINKKWKEAVLSEPD-VTVHDLYEKYRDQPIDVEFEQQQLLAHDR 62 Query: 61 IVLQFPIQWYSMPALLKQWLDGVFTTSWLYETGGKAVAGKELVLAMSIGGDESTYQSGGM 120 IV QFP+ WYS P LLKQW D VFT W + GG + GKE V AMSIG E +YQ+GG Sbjct: 63 IVFQFPLYWYSSPPLLKQWFDEVFTFGWAHGPGGNKLKGKEWVTAMSIGSPEHSYQAGGY 122 Query: 121 IGFTISELIRPVQVFSNQVGMTFLPHYKFYGANKATEDQIDNSAQRYVEHITSPDLNPVV 180 F+ISEL +P Q ++ VGMT+LP + Y AN ++ +I SA RYV+HIT+ +LNP V Sbjct: 123 NLFSISELTKPFQASAHLVGMTYLPSFAEYRANTISDQEIAESANRYVKHITNIELNPKV 182 Query: 181 VRKRMLAEM 189 +R L ++ Sbjct: 183 RLQRYLKQL 191 >P54439.2 YRKL_BACSU Uncharacterized NAD(P)H oxidoreductase yrkL EC=1.6.99.- Length = 174 Score = 180 bits (457), Expect = 6e-44, Method: Compositional matrix adjust. Identities = 86/173 (49%), Positives = 116/173 (67%), Gaps = 1/173 (0%) Query: 1 MSILIVAAHPRIEHSRVNKRWLSELRGYPDQVTVHELYKAYPHFTIHAENEQDLVARYDR 60 M L++ HP +E S VNK W++ L+ D +TVH+LY YP+F I E EQ L+ ++R Sbjct: 1 MKTLVIVIHPNLETSVVNKTWMNRLKQEKD-ITVHDLYGEYPNFIIDVEKEQQLLLDHER 59 Query: 61 IVLQFPIQWYSMPALLKQWLDGVFTTSWLYETGGKAVAGKELVLAMSIGGDESTYQSGGM 120 IV QFP+ WYS PALLKQW D V T W Y TGG + GKEL+LA+S+G ES YQ+GG Sbjct: 60 IVFQFPMYWYSSPALLKQWEDDVLTHGWAYGTGGTKLHGKELLLAISLGAQESDYQAGGE 119 Query: 121 IGFTISELIRPVQVFSNQVGMTFLPHYKFYGANKATEDQIDNSAQRYVEHITS 173 TISELIRP QV +N +GM FLP + YG +++ + NSA+R V+++ + Sbjct: 120 YNITISELIRPFQVTANYIGMRFLPAFTQYGTLHLSKEDVKNSAERLVDYLKA 172 >Q8VSR5.1 Q8VSR5_BACAM Nitroreductase Length = 174 Score = 180 bits (456), Expect = 6e-44, Method: Compositional matrix adjust. Identities = 85/171 (49%), Positives = 114/171 (66%), Gaps = 1/171 (0%) Query: 1 MSILIVAAHPRIEHSRVNKRWLSELRGYPDQVTVHELYKAYPHFTIHAENEQDLVARYDR 60 M +L++A HP +E+S VNK W EL+ + D++TV ELYK YP I AE E+ L +YDR Sbjct: 1 MKVLVLAVHPDMENSAVNKAWAEELKKH-DELTVRELYKEYPDGQIDAEKERQLCEQYDR 59 Query: 61 IVLQFPIQWYSMPALLKQWLDGVFTTSWLYETGGKAVAGKELVLAMSIGGDESTYQSGGM 120 IV QFP+ WYS P LLK W+D V + W Y + GKA+ GKEL+LA+S+G E YQ+GG Sbjct: 60 IVFQFPLYWYSAPPLLKTWMDHVLSYGWAYGSKGKALHGKELMLAVSVGAGEDAYQAGGS 119 Query: 121 IGFTISELIRPVQVFSNQVGMTFLPHYKFYGANKATEDQIDNSAQRYVEHI 171 FT+SEL+RP Q +N GMT+LP + YG N A I ++A+R +I Sbjct: 120 NHFTLSELLRPFQAMANFTGMTYLPAFALYGVNGADATDIHDNAKRLAAYI 170 >A7Z9G9.1 A7Z9G9_BACA2 YwrO Length = 177 Score = 177 bits (448), Expect = 5e-43, Method: Compositional matrix adjust. Identities = 82/174 (47%), Positives = 114/174 (65%), Gaps = 1/174 (0%) Query: 1 MSILIVAAHPRIEHSRVNKRWLSELRGYPDQVTVHELYKAYPHFTIHAENEQDLVARYDR 60 + +L++A HP + +S VNK W EL+ + D++TV ELYK YP I AE E+ L +YDR Sbjct: 4 VKVLVLAIHPDMNNSAVNKAWTEELKNH-DEITVRELYKEYPDGKIDAEKERQLCEQYDR 62 Query: 61 IVLQFPIQWYSMPALLKQWLDGVFTTSWLYETGGKAVAGKELVLAMSIGGDESTYQSGGM 120 IV QFP+ WYS P LLK W+D V W Y + GKA+ GKEL+LA+S+G E YQ+GG Sbjct: 63 IVFQFPLYWYSAPPLLKTWMDHVLAYGWAYGSKGKALHGKELLLAVSVGAGEDAYQAGGS 122 Query: 121 IGFTISELIRPVQVFSNQVGMTFLPHYKFYGANKATEDQIDNSAQRYVEHITSP 174 F++SEL+RP Q +N GMT+LP + YG + A I ++A+R ++I P Sbjct: 123 NHFSLSELLRPFQAMANFTGMTYLPAFAMYGVSSADAAAIRDNAKRLADYIKKP 176 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.134 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,226,314,987 Number of extensions: 48848257 Number of successful extensions: 135147 Number of sequences better than 1.0e-05: 846 Number of HSP's gapped: 134543 Number of HSP's successfully gapped: 846 Length of query: 197 Length of database: 3,060,696,203 Length adjustment: 129 Effective length of query: 68 Effective length of database: 1,845,385,268 Effective search space: 125486198224 Effective search space used: 125486198224 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 127 (53.5 bits)