BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0557.0028.p (435 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KBH4.1 B3KBH4_9BACI Glutamate dehydrogenase (NADP(+)) EC=1.4.1.4 681 0.0 Q9KB34.1 Q9KB34_BACHD NADP-specific glutamate dehydrogenase EC=1... 593 e-167 Q5WCI5.1 Q5WCI5_BACSK NADP-specific glutamate dehydrogenase EC=1... 591 e-167 O87403.1 O87403_BACLI NADP-specific glutamate dehydrogenase EC=1... 588 e-166 Q65GI2.1 Q65GI2_BACLD RocG 577 e-163 >B3KBH4.1 B3KBH4_9BACI Glutamate dehydrogenase (NADP(+)) EC=1.4.1.4 Length = 493 Score = 681 bits (1757), Expect = 0.0, Method: Compositional matrix adjust. Identities = 324/435 (74%), Positives = 359/435 (82%) Query: 1 VITRDPHEREFHQAVKEILDTLVPVLARHPRYREHGILERMVEPERSITFRVPWVDDAGT 60 V+ RDPHE EFHQAV+E +D+LVPVLA+HP+YREHGILER+VEPER +TFRVPWVDD G Sbjct: 59 VVARDPHESEFHQAVREFVDSLVPVLAQHPKYREHGILERLVEPERMVTFRVPWVDDQGK 118 Query: 61 IQVNRGYRIQFNSAIGPYKGGIRFHPSVYSGIIKFLGFEQIFKNALTXXXXXXXXXXXXX 120 QVNRG+R+QFNSA+GPYKGGIRFHPSVY+GI+KFLGFEQIFKN+LT Sbjct: 119 TQVNRGFRVQFNSALGPYKGGIRFHPSVYAGIVKFLGFEQIFKNSLTGQPIGGGKGGSDF 178 Query: 121 XXXXXXXQEIMRFTQSFMTELYRHIGPDSDVPAGDIGVGAREIGYMFGQYKRIRGGHEAG 180 QE+MRFTQSFMTELYRHIGPDSDVPAGDIGVGAREIGYMFGQYKRIRGGHEAG Sbjct: 179 DPKGKSDQEVMRFTQSFMTELYRHIGPDSDVPAGDIGVGAREIGYMFGQYKRIRGGHEAG 238 Query: 181 VLTGKGLQYGGSLARKEATGYGCVYFVQEMLAAKGLGFRDNRXXXXXXXXXXIYAIEKAQ 240 VLTGKG+ YGGSLAR EATGYGCVYFVQEMLAAKGL F+D+R IYAIEKAQ Sbjct: 239 VLTGKGVIYGGSLARTEATGYGCVYFVQEMLAAKGLSFQDSRVVVSGSGNVSIYAIEKAQ 298 Query: 241 QMGASVVACSDSGGYIYDPLGINLETVKRLKETQQLRISEYVKIHPHAEYFAGCAGIWNL 300 Q+GA V+ACSDS GY+YDP GINLETVK LKE +LRISEYVKIHPHA Y GC GIW+L Sbjct: 299 QLGAHVIACSDSNGYLYDPEGINLETVKLLKERDRLRISEYVKIHPHAVYTEGCTGIWSL 358 Query: 301 PCDIALPCATQNELDEDAARKLINNGVKAIGEGANMPSTLQAIQLFLDNAVLFXXXXXXX 360 PCDIALPCATQNELD DAA+ LI+NGVKAIGEGANMPSTL+AI+LF+ VLF Sbjct: 359 PCDIALPCATQNELDADAAKTLISNGVKAIGEGANMPSTLEAIELFISKGVLFGPAKAAN 418 Query: 361 XXXXXXXXLEMSQNSMRIAWTFEEVDEKLQSIMKNIYRSAVEAAEEYEVPGNLLAGANIA 420 LEMSQNSMR++WTFEEVD KL IMKNIY SAV+AAEEY V GNL+AGANIA Sbjct: 419 AGGVAVSALEMSQNSMRMSWTFEEVDAKLHQIMKNIYNSAVDAAEEYGVAGNLVAGANIA 478 Query: 421 GFLRVADAMLAQGIV 435 GF++VADAM+AQGIV Sbjct: 479 GFIKVADAMIAQGIV 493 >Q9KB34.1 Q9KB34_BACHD NADP-specific glutamate dehydrogenase EC=1.4.1.4 Length = 458 Score = 593 bits (1529), Expect = e-167, Method: Compositional matrix adjust. Identities = 281/432 (65%), Positives = 332/432 (76%) Query: 4 RDPHEREFHQAVKEILDTLVPVLARHPRYREHGILERMVEPERSITFRVPWVDDAGTIQV 63 R+P+E EFHQAVKE+ D+L+PVL +HP+Y + ILER+VEPER I+FRVPWVDD G +QV Sbjct: 27 RNPNEHEFHQAVKEVFDSLLPVLVKHPQYVKQAILERIVEPERVISFRVPWVDDQGNVQV 86 Query: 64 NRGYRIQFNSAIGPYKGGIRFHPSVYSGIIKFLGFEQIFKNALTXXXXXXXXXXXXXXXX 123 NRG+R+QFNSA+GPYKGG+RFHPSV + IIKFLGFEQIFKNALT Sbjct: 87 NRGFRVQFNSALGPYKGGLRFHPSVNASIIKFLGFEQIFKNALTGQPIGGGKGGSDFDPK 146 Query: 124 XXXXQEIMRFTQSFMTELYRHIGPDSDVPAGDIGVGAREIGYMFGQYKRIRGGHEAGVLT 183 EIMRF+QSFM+EL +IGPD DVPAGDIGVGA+EIGYMFGQYK++RGG EAGVLT Sbjct: 147 GKSDGEIMRFSQSFMSELSNYIGPDIDVPAGDIGVGAKEIGYMFGQYKKMRGGFEAGVLT 206 Query: 184 GKGLQYGGSLARKEATGYGCVYFVQEMLAAKGLGFRDNRXXXXXXXXXXIYAIEKAQQMG 243 GKG+ YGGSLARKEATGYG VYFV+EM+ G F + IYA+EKA Q+G Sbjct: 207 GKGIGYGGSLARKEATGYGTVYFVEEMIKDHGFSFAGSTVVVSGSGNVSIYAMEKAMQLG 266 Query: 244 ASVVACSDSGGYIYDPLGINLETVKRLKETQQLRISEYVKIHPHAEYFAGCAGIWNLPCD 303 A VVACSDSGGY+YD GI+L+TVKRLKE ++ RISEYV HPHA Y GC+GIW++PCD Sbjct: 267 AKVVACSDSGGYVYDKNGIDLQTVKRLKEVERKRISEYVNEHPHAHYVQGCSGIWSVPCD 326 Query: 304 IALPCATQNELDEDAARKLINNGVKAIGEGANMPSTLQAIQLFLDNAVLFXXXXXXXXXX 363 IALPCATQNELDE AA LI NGVKA+GEGANMPSTLQA+ F ++ VLF Sbjct: 327 IALPCATQNELDEAAATMLIANGVKAVGEGANMPSTLQAVHTFQEHGVLFAPAKAANAGG 386 Query: 364 XXXXXLEMSQNSMRIAWTFEEVDEKLQSIMKNIYRSAVEAAEEYEVPGNLLAGANIAGFL 423 LEM+QNS R+AWTFEEVD KL IMKNIYR +++AAE YE GNL+ GANIAGF+ Sbjct: 387 VSVSALEMAQNSTRLAWTFEEVDAKLYEIMKNIYRESIKAAELYEASGNLVVGANIAGFV 446 Query: 424 RVADAMLAQGIV 435 +VADAM++ G+V Sbjct: 447 KVADAMISHGVV 458 >Q5WCI5.1 Q5WCI5_BACSK NADP-specific glutamate dehydrogenase EC=1.4.1.4 Length = 460 Score = 591 bits (1524), Expect = e-167, Method: Compositional matrix adjust. Identities = 280/435 (64%), Positives = 335/435 (77%) Query: 1 VITRDPHEREFHQAVKEILDTLVPVLARHPRYREHGILERMVEPERSITFRVPWVDDAGT 60 V R+P+E EFHQAVKE+LD+L+PVLA+ P+Y ILER+VEPER I+FRVPWVDD G Sbjct: 26 VKQRNPYENEFHQAVKEVLDSLLPVLAKRPQYIRQAILERIVEPERMISFRVPWVDDQGN 85 Query: 61 IQVNRGYRIQFNSAIGPYKGGIRFHPSVYSGIIKFLGFEQIFKNALTXXXXXXXXXXXXX 120 +QVNRG+R+QFNSA+GPYKGG+RFHPSV + IIKFLGFEQIFKNALT Sbjct: 86 VQVNRGFRVQFNSALGPYKGGLRFHPSVNTSIIKFLGFEQIFKNALTGQPIGGGKGGSDF 145 Query: 121 XXXXXXXQEIMRFTQSFMTELYRHIGPDSDVPAGDIGVGAREIGYMFGQYKRIRGGHEAG 180 EIMRFTQSFM+EL ++IGPD+DVPAGDIGVGA+EIGYMFGQYK++RG EAG Sbjct: 146 DPKGKSDGEIMRFTQSFMSELSKYIGPDTDVPAGDIGVGAKEIGYMFGQYKKMRGRFEAG 205 Query: 181 VLTGKGLQYGGSLARKEATGYGCVYFVQEMLAAKGLGFRDNRXXXXXXXXXXIYAIEKAQ 240 VLTGK + YGGSLARKEATGYG VYFV+EML KG F + IYA+EKA Sbjct: 206 VLTGKDIAYGGSLARKEATGYGTVYFVEEMLKEKGHSFAGSTVVVSGSGNVSIYAMEKAM 265 Query: 241 QMGASVVACSDSGGYIYDPLGINLETVKRLKETQQLRISEYVKIHPHAEYFAGCAGIWNL 300 +GA VVACSDS GYIYD GINLETVKRLKE+++ RISEYV HP A +F GC+ IW++ Sbjct: 266 HLGAKVVACSDSEGYIYDKRGINLETVKRLKESEKKRISEYVNEHPQAHFFQGCSDIWSV 325 Query: 301 PCDIALPCATQNELDEDAARKLINNGVKAIGEGANMPSTLQAIQLFLDNAVLFXXXXXXX 360 PCDIALPCATQNE+DE+ A L++NGVKAIGEGANMPSTL+A+ +F ++ VLF Sbjct: 326 PCDIALPCATQNEIDENTATVLVSNGVKAIGEGANMPSTLEAVNVFHEHGVLFAPAKAAN 385 Query: 361 XXXXXXXXLEMSQNSMRIAWTFEEVDEKLQSIMKNIYRSAVEAAEEYEVPGNLLAGANIA 420 LEM+QNS R++WTFEEVD KLQ IMK IYR +EAA+E + PGNL+ GANIA Sbjct: 386 AGGVSVSALEMAQNSARLSWTFEEVDAKLQDIMKTIYRDCIEAAKENDAPGNLVVGANIA 445 Query: 421 GFLRVADAMLAQGIV 435 GF++VADAM+AQG++ Sbjct: 446 GFVKVADAMIAQGVI 460 >O87403.1 O87403_BACLI NADP-specific glutamate dehydrogenase EC=1.4.1.4 Length = 460 Score = 588 bits (1516), Expect = e-166, Method: Compositional matrix adjust. Identities = 286/435 (65%), Positives = 330/435 (75%) Query: 1 VITRDPHEREFHQAVKEILDTLVPVLARHPRYREHGILERMVEPERSITFRVPWVDDAGT 60 V R+ HE EF QAVKEI D+LVPVLARHP+Y EH ILER+ EPER ITFRVPWVDD G Sbjct: 26 VRQRNLHESEFLQAVKEIFDSLVPVLARHPKYIEHRILERIAEPERMITFRVPWVDDEGN 85 Query: 61 IQVNRGYRIQFNSAIGPYKGGIRFHPSVYSGIIKFLGFEQIFKNALTXXXXXXXXXXXXX 120 IQVNRGYR+QFNSAIGPYKGGIRFHPSV + IIKFLGFEQIFKN+LT Sbjct: 86 IQVNRGYRVQFNSAIGPYKGGIRFHPSVNASIIKFLGFEQIFKNSLTGQPIGGGKGGADF 145 Query: 121 XXXXXXXQEIMRFTQSFMTELYRHIGPDSDVPAGDIGVGAREIGYMFGQYKRIRGGHEAG 180 +EIM FTQSFM ELYRHIGPD+D+PAGDIGVGARE+G+MFGQYK+IRG ++AG Sbjct: 146 DPKGKSDREIMSFTQSFMNELYRHIGPDTDIPAGDIGVGAREVGFMFGQYKKIRGRYDAG 205 Query: 181 VLTGKGLQYGGSLARKEATGYGCVYFVQEMLAAKGLGFRDNRXXXXXXXXXXIYAIEKAQ 240 VLTGKGL+YGGSL RKEATGYG VYFV+EML +GL F ++ +YA+EKA Sbjct: 206 VLTGKGLEYGGSLTRKEATGYGLVYFVEEMLKDQGLRFENSTVVVSGSGNVALYAMEKAA 265 Query: 241 QMGASVVACSDSGGYIYDPLGINLETVKRLKETQQLRISEYVKIHPHAEYFAGCAGIWNL 300 Q G VVACSDS GY+YD GI+LETVKRLKE+ RI EYV HP A YF GC+GIW++ Sbjct: 266 QFGGKVVACSDSDGYVYDEKGISLETVKRLKESGNGRIREYVSEHPEAHYFEGCSGIWSI 325 Query: 301 PCDIALPCATQNELDEDAARKLINNGVKAIGEGANMPSTLQAIQLFLDNAVLFXXXXXXX 360 PCDIALPCATQNE+DE+AA LI+NGVKA+GEGANMPS A++ FLD VLF Sbjct: 326 PCDIALPCATQNEIDEEAAGVLISNGVKAVGEGANMPSEEGAVKRFLDAGVLFGPAKAAN 385 Query: 361 XXXXXXXXLEMSQNSMRIAWTFEEVDEKLQSIMKNIYRSAVEAAEEYEVPGNLLAGANIA 420 LEM+QNS R+ WT EE DEKL+ IM +IY+ +VEAA EY PGNLL G NIA Sbjct: 386 AGGVAVSALEMAQNSARLHWTAEETDEKLRMIMTDIYKRSVEAASEYGRPGNLLDGCNIA 445 Query: 421 GFLRVADAMLAQGIV 435 GF++VADAM+AQG+V Sbjct: 446 GFIKVADAMIAQGVV 460 >Q65GI2.1 Q65GI2_BACLD RocG Length = 460 Score = 577 bits (1487), Expect = e-163, Method: Compositional matrix adjust. Identities = 281/435 (64%), Positives = 328/435 (75%) Query: 1 VITRDPHEREFHQAVKEILDTLVPVLARHPRYREHGILERMVEPERSITFRVPWVDDAGT 60 V R+ +E EF QAVKEI D+LVPVLARHP+Y EH ILER+ EPER ITFRVPWVDD G Sbjct: 26 VQKRNFYESEFLQAVKEIFDSLVPVLARHPKYIEHRILERIAEPERMITFRVPWVDDEGN 85 Query: 61 IQVNRGYRIQFNSAIGPYKGGIRFHPSVYSGIIKFLGFEQIFKNALTXXXXXXXXXXXXX 120 I+VNRG+R+QFNSAIGPYKGGIRFHPSV + IIKFLGFEQIFKN+LT Sbjct: 86 IRVNRGFRVQFNSAIGPYKGGIRFHPSVNASIIKFLGFEQIFKNSLTGLPIGGGKGGADF 145 Query: 121 XXXXXXXQEIMRFTQSFMTELYRHIGPDSDVPAGDIGVGAREIGYMFGQYKRIRGGHEAG 180 +EIM FTQSFM ELYRHIGPD+D+PAGDIGVGARE+G+MFGQYK+IRG ++AG Sbjct: 146 DPKGKSDREIMSFTQSFMNELYRHIGPDTDIPAGDIGVGAREVGFMFGQYKKIRGRYDAG 205 Query: 181 VLTGKGLQYGGSLARKEATGYGCVYFVQEMLAAKGLGFRDNRXXXXXXXXXXIYAIEKAQ 240 VLTGKGL+YGGSL RKEATGYG VYFV+EML +G+ F ++ +YA+EKA Sbjct: 206 VLTGKGLEYGGSLTRKEATGYGLVYFVEEMLKDQGMRFENSTVVVSGSGNVALYAMEKAA 265 Query: 241 QMGASVVACSDSGGYIYDPLGINLETVKRLKETQQLRISEYVKIHPHAEYFAGCAGIWNL 300 Q GA VVACSDS GY+YD GI LETVKRLKE RI EYV HP A YF GC GIW++ Sbjct: 266 QFGAKVVACSDSDGYVYDEKGICLETVKRLKEDGNGRIREYVSEHPEAHYFEGCTGIWSI 325 Query: 301 PCDIALPCATQNELDEDAARKLINNGVKAIGEGANMPSTLQAIQLFLDNAVLFXXXXXXX 360 PCDIALPCATQNE+DE+AA LI+NGVKA+GEGANMPS A++ FLD VLF Sbjct: 326 PCDIALPCATQNEIDEEAAEVLISNGVKAVGEGANMPSEEGAVKRFLDAGVLFGPAKAAN 385 Query: 361 XXXXXXXXLEMSQNSMRIAWTFEEVDEKLQSIMKNIYRSAVEAAEEYEVPGNLLAGANIA 420 LEM+QNS R+ WT EE D KL++IM +I++ +VEAA EY PGNLL G NIA Sbjct: 386 AGGVAVSALEMAQNSARLHWTAEETDAKLRAIMADIHKRSVEAASEYGRPGNLLDGCNIA 445 Query: 421 GFLRVADAMLAQGIV 435 GF++VADAM+AQG+V Sbjct: 446 GFIKVADAMIAQGVV 460 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.139 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,266,461,017 Number of extensions: 86963133 Number of successful extensions: 198133 Number of sequences better than 1.0e-05: 1961 Number of HSP's gapped: 191837 Number of HSP's successfully gapped: 1966 Length of query: 435 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 296 Effective length of database: 1,751,175,118 Effective search space: 518347834928 Effective search space used: 518347834928 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)