BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0557.0020.p (290 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B4VCI0.1 B4VCI0_9ACTO Hydrolase 123 2e-26 Q0W6N9.1 Q0W6N9_UNCMA Putative hydrolase (Alpha/beta fold family) 117 2e-24 Q9K3V0.1 Q9K3V0_STRCO Putative hydrolase 116 2e-24 B5HLA7.1 B5HLA7_9ACTO Hydrolase 115 4e-24 Q82GP3.1 Q82GP3_STRAW Putative hydrolase 112 4e-23 >B4VCI0.1 B4VCI0_9ACTO Hydrolase Length = 298 Score = 123 bits (308), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 84/270 (31%), Positives = 127/270 (47%), Gaps = 33/270 (12%) Query: 36 EGPAVLCLHGRWGRAETWVDFIHQYGKQFRVIAPDQRGHGLSSKPVSN-YTAEEMAEDIK 94 E PAVL LHG GRA W ++ RV+A DQRGHG S +P + YT E D + Sbjct: 42 ERPAVLLLHGLMGRAFHWAGTARWLAERHRVVALDQRGHGQSDRPATGPYTREAFVADAE 101 Query: 95 ELIIHLNLESVIIVGHSMGGGVAGYMAALHPEYIQALAILDKSANGPDERNPVSLEQIPA 154 +I L L V ++GHSMG A +AA HP+ ++AL I D A+ E + E+ Sbjct: 102 AVIEQLGLAPVTLIGHSMGALTAWQLAARHPDLVRALVICDMRASALGEASQQEWEEW-- 159 Query: 155 VDPVTKDWPLPFSSLCEAKHYI-------------RGAMVSELSYQYFINSLIETPEGYE 201 WPLPF + A+ + RGA +E+ +Q P+G+ Sbjct: 160 ----FHRWPLPFPTQDAARRWFGEDDPRVERPDPGRGAFFAEVMHQ--------APDGWR 207 Query: 202 MMFSPQAIAANIAYYV---NWYSLLPSIKCPVLLIRAKNSEGLPDADYMKMQSLLPNCHA 258 +FS + + +V +W L ++CP L++R + E L A+ +M +LP Sbjct: 208 PLFSRRQMLTARETWVHDAHWEELA-QVRCPTLVVRGLDGE-LGRAEAQEMVRVLPAGQY 265 Query: 259 YEISHPDHNVHLGNKEEFYTCMDLFLDGLR 288 EI H +H + ++ FLDG++ Sbjct: 266 AEIPDAGHYLHYDQPTAWRAALEPFLDGVK 295 >Q0W6N9.1 Q0W6N9_UNCMA Putative hydrolase (Alpha/beta fold family) Length = 260 Score = 117 bits (292), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 79/271 (29%), Positives = 125/271 (46%), Gaps = 22/271 (8%) Query: 18 RSIAKLTHIDMFYMDTKTEGPAVLCLHGRWGRAETWVDFIHQYGKQFRVIAPDQRGHGLS 77 R L + + Y+D +G +L LHG +G A ++ ++RVIA DQRGHG S Sbjct: 4 RQFVDLDGVRLSYVDYGGKGEPLLALHGHYGCARSFAGLAEALHNKWRVIALDQRGHGWS 63 Query: 78 SKPVSNYTAEEMAEDIKELIIHLNLESVIIVGHSMGGGVAGYMAALHPEYIQALAILDKS 137 +P +Y+ E D + LNL +++GHS+GG A +AA P+ ++AL I D Sbjct: 64 EQP-DDYSREAYVRDAAIFLKKLNLGPAVVLGHSLGGLNAYQLAAWQPDLVKALIIEDIG 122 Query: 138 ANGPDERNPVSLEQIPAVDPVTKDWPLPFSSLCEAKHYIRGAMVSELSYQ---YFINSLI 194 A +P P T WPL F S+ +H + A ++ ++ YF SL+ Sbjct: 123 A------------VVPRFGPATHGWPLRFDSM---RHVLEWA--EQMGFKNDSYFQESLV 165 Query: 195 ETPEGYEMMFSPQAIAANIAYYV-NWYSLLPSIKCPVLLIRAKNSEGLPDADYMKMQSLL 253 E P+G+ F+ +A ++ +W+ + KCP LL+ S L M + Sbjct: 166 EYPDGWGFRFNYDKLARSMELIAGDWWQDWLASKCPALLVHGHRSWVLGTDHARDMAAKR 225 Query: 254 PNCHAYEISHPDHNVHLGNKEEFYTCMDLFL 284 PN E H +H +F+ + FL Sbjct: 226 PNTKLVEFPECGHTIHDEEPVKFHRSVVQFL 256 >Q9K3V0.1 Q9K3V0_STRCO Putative hydrolase Length = 352 Score = 116 bits (291), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 72/259 (27%), Positives = 126/259 (48%), Gaps = 17/259 (6%) Query: 38 PAVLCLHGRWGRAETWVDFIHQYGKQFRVIAPDQRGHGLSSK-PVSNYTAEEMAEDIKEL 96 P VL LHG GRA W + R +A DQRGHG S K P + YT E ED++ Sbjct: 99 PGVLLLHGLMGRASHWAPTARWLSARHRAVALDQRGHGRSDKPPRAAYTREAYVEDVEAA 158 Query: 97 IIHLNLESVIIVGHSMGGGVAGYMAALHPEYIQALAILDKSANGPDERNPVSLEQIPAVD 156 + L L +++GH+MG A +AA P+ ++ L I D A+ + + + Sbjct: 159 LEQLGLGPAVLIGHAMGALTAWQLAAKRPDLVRGLIICDMRASALGAASQHTWTEW---- 214 Query: 157 PVTKDWPLPFSSLCEAKHYIRG-----AMVSELSYQYFINSLIETPEGYEMMFSPQAIAA 211 + WP+PF++L + + + + +++ + E+P+G+ +F P+ + Sbjct: 215 --FRAWPVPFATLADVRKWFGEDDPWVERPNPARGEFYAEVMAESPDGWRPVFEPEQMLR 272 Query: 212 NIAYYV---NWYSLLPSIKCPVLLIRAKNSEGLPDADYMKMQSLLPNCHAYEISHPDHNV 268 + +V +W L ++CP L++R + E L A+ +M +LP+ E++ H V Sbjct: 273 SRETWVYDAHWEELA-QVRCPTLVVRGLDGE-LGRAEAQEMVRVLPSGQYAEVADAGHLV 330 Query: 269 HLGNKEEFYTCMDLFLDGL 287 H E + T ++ FL+ L Sbjct: 331 HYDQPEAWRTAIEPFLNAL 349 >B5HLA7.1 B5HLA7_9ACTO Hydrolase Length = 311 Score = 115 bits (289), Expect = 4e-24, Method: Compositional matrix adjust. Identities = 70/259 (27%), Positives = 126/259 (48%), Gaps = 17/259 (6%) Query: 38 PAVLCLHGRWGRAETWVDFIHQYGKQFRVIAPDQRGHGLSSK-PVSNYTAEEMAEDIKEL 96 P VL LHG GRA W +++R +A DQRGHG S K P + +T + ED + Sbjct: 52 PGVLLLHGLMGRASHWASTARWLSERYRAVALDQRGHGQSDKPPRAAFTRDAYVEDAEAA 111 Query: 97 IIHLNLESVIIVGHSMGGGVAGYMAALHPEYIQALAILDKSANGPDERNPVSLEQIPAVD 156 + L L +++GH+MG A +AA P+ ++ + I D A+ + + Sbjct: 112 LEQLGLAPTVLIGHAMGALTAWQLAARRPDLVRGVIICDMRASALGAASQREWGEW---- 167 Query: 157 PVTKDWPLPFSSLCEAKHYIRG-----AMVSELSYQYFINSLIETPEGYEMMFSPQAIAA 211 + WP+PF++L + + + + +++ + E+P+G+ +F P+ + + Sbjct: 168 --FEAWPVPFATLADVRKWFGEDDPWVERPNPARGEFYAEVMAESPDGWRPVFEPEQMLS 225 Query: 212 NIAYYV---NWYSLLPSIKCPVLLIRAKNSEGLPDADYMKMQSLLPNCHAYEISHPDHNV 268 + +V +W L ++CP L++R + E L A+ +M +LP E++ H V Sbjct: 226 SRETWVYDAHWEELT-QVRCPALVVRGLDGE-LGRAEAQEMVRVLPRGEYAEVADAGHLV 283 Query: 269 HLGNKEEFYTCMDLFLDGL 287 H E + ++ FLDGL Sbjct: 284 HYDQPEAWRAAIEPFLDGL 302 >Q82GP3.1 Q82GP3_STRAW Putative hydrolase Length = 307 Score = 112 bits (280), Expect = 4e-23, Method: Compositional matrix adjust. Identities = 75/267 (28%), Positives = 123/267 (46%), Gaps = 33/267 (12%) Query: 38 PAVLCLHGRWGRAETWVDFIHQYGKQFRVIAPDQRGHGLSSK-PVSNYTAEEMAEDIKEL 96 P VL LHG GRA W ++ R +A DQRGHG S K P + YT E ED + Sbjct: 54 PGVLLLHGLMGRASHWASTARWLSERHRAVALDQRGHGQSGKPPEAAYTREAYVEDAEAA 113 Query: 97 IIHLNLESVIIVGHSMGGGVAGYMAALHPEYIQALAILDKSANGPDERNPVSLEQIPAVD 156 + L L +++GH+MG A +AA P+ + L I D A+ + E Sbjct: 114 LEQLGLAPTVLIGHAMGALTAWQLAAKRPDLVCGLIICDMRASALGAASQREWEDW---- 169 Query: 157 PVTKDWPLPFSSLCEAKHYI-------------RGAMVSELSYQYFINSLIETPEGYEMM 203 K WP+PF++L + + + RG +++ + E+ +G+ + Sbjct: 170 --FKAWPVPFATLADVRKWFGEDDPWVERPNPSRG--------EFYAEVMHESDDGWRPV 219 Query: 204 FSPQAIAANIAYYV---NWYSLLPSIKCPVLLIRAKNSEGLPDADYMKMQSLLPNCHAYE 260 F P+ + + +V +W L ++CP L++R + E L A+ +M +LP E Sbjct: 220 FEPEQMLKSRQTWVYDAHWEELA-QVRCPALVVRGLDGE-LGRAESQEMVRVLPYGQYAE 277 Query: 261 ISHPDHNVHLGNKEEFYTCMDLFLDGL 287 ++ H VH E + ++ FLDG+ Sbjct: 278 VAEAGHLVHYDQPEAWRAAIEPFLDGV 304 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.136 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,786,600,140 Number of extensions: 73024396 Number of successful extensions: 200039 Number of sequences better than 1.0e-05: 3415 Number of HSP's gapped: 202122 Number of HSP's successfully gapped: 3416 Length of query: 290 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 156 Effective length of database: 1,798,280,193 Effective search space: 280531710108 Effective search space used: 280531710108 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)