BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0555.0008.p (340 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K792.1 B3K792_9BACI dTDP-glucose 4,6-dehydratase EC=4.2.1.46 599 e-169 Q93F85.1 Q93F85_ANETH dTDP-glucose 4,6-dehydratase EC=4.2.1.46 543 e-153 Q7BG47.1 Q7BG47_BACST dTDP-glucose 4,6-dehydratase EC=4.2.1.46 540 e-151 A2BD31.1 A2BD31_9BACI dTDP-glucose 4,6-dehydratase EC=4.2.1.46 535 e-150 B1D5Q1.1 B1D5Q1_9BACL dTDP-glucose 4,6-dehydratase EC=4.2.1.46 529 e-148 >B3K792.1 B3K792_9BACI dTDP-glucose 4,6-dehydratase EC=4.2.1.46 Length = 341 Score = 599 bits (1545), Expect = e-169, Method: Compositional matrix adjust. Identities = 284/338 (84%), Positives = 313/338 (92%), Gaps = 1/338 (0%) Query: 1 MKLLVTGGAGFIGSNFVIYMLQQHPEYKIINVDALTYAGNLENLKSIENHPNYTFAKADI 60 MKLLVTGGAGFIGSNFVIYMLQQ+P+Y+I+N+DALTYAGNLENLKS+EN+ NYTF ADI Sbjct: 1 MKLLVTGGAGFIGSNFVIYMLQQYPKYEIVNMDALTYAGNLENLKSVENNANYTFINADI 60 Query: 61 TDVQAMDVLFSQ-GVDVVVNFAAESHVDRSILEPDVFVKTNVLGTQVLLDAAKKYNVTKF 119 + +A++ +F Q ++VVVNFAAESHVDRSILEP+VFV TNVLGTQVLLDAAKK+NVTKF Sbjct: 61 ANKEAVEQIFEQHQIEVVVNFAAESHVDRSILEPEVFVNTNVLGTQVLLDAAKKHNVTKF 120 Query: 120 VQVSTDEVYGTLGATGLFTEETPLTPNSPYSASKAGGDLLVRAYHETFGLPVNITRCSNN 179 VQVSTDEVYG+LG TGLFTEETPL PNSPYSASKAGGDLLVRAYHETFGLPVNITRCSNN Sbjct: 121 VQVSTDEVYGSLGETGLFTEETPLAPNSPYSASKAGGDLLVRAYHETFGLPVNITRCSNN 180 Query: 180 YGPYQFPEKLIPLMISRALADQALPVYGDGMNIRDWLYVEDHCSAIDLVIHEGVNGEVYN 239 YGP QFPEKLIPLMISRAL D+ALPVYGDG+NIRDWLYVEDHCSAIDLVIH+GV+GEVYN Sbjct: 181 YGPLQFPEKLIPLMISRALNDEALPVYGDGLNIRDWLYVEDHCSAIDLVIHKGVSGEVYN 240 Query: 240 IGGNNERTNVHIVKTVLQELGKPDSLITYVQDRPGHDRRYGIDPTKITRELGWKPKHTFE 299 IGGNNERTNVHIV+ +LQELGKP+SLI+YV DRPGHDRRYGIDPTKI ELGWKPKH FE Sbjct: 241 IGGNNERTNVHIVQKILQELGKPESLISYVDDRPGHDRRYGIDPTKIMNELGWKPKHNFE 300 Query: 300 TGIKETIQWYLNNQEWWTRIQSGEYQKYAELQYGSRLG 337 TGIKETIQWYL+N+EWW RIQSG Y++Y QYG RLG Sbjct: 301 TGIKETIQWYLDNKEWWQRIQSGVYREYVTKQYGKRLG 338 >Q93F85.1 Q93F85_ANETH dTDP-glucose 4,6-dehydratase EC=4.2.1.46 Length = 341 Score = 543 bits (1400), Expect = e-153, Method: Compositional matrix adjust. Identities = 251/336 (74%), Positives = 290/336 (86%) Query: 1 MKLLVTGGAGFIGSNFVIYMLQQHPEYKIINVDALTYAGNLENLKSIENHPNYTFAKADI 60 MK+LVTGGAGFIGSNFV YM+ ++P Y +NVDALTYAGNLENLK +EN PNYTF KADI Sbjct: 1 MKVLVTGGAGFIGSNFVNYMVHKYPNYTFVNVDALTYAGNLENLKPVENAPNYTFVKADI 60 Query: 61 TDVQAMDVLFSQGVDVVVNFAAESHVDRSILEPDVFVKTNVLGTQVLLDAAKKYNVTKFV 120 TD + M+ L + GVDV+VNFAAESHVDRSI EPD FVKTN++GTQ+LLD A+K N+ K+V Sbjct: 61 TDREKMEKLVADGVDVIVNFAAESHVDRSITEPDTFVKTNIMGTQILLDIARKCNINKYV 120 Query: 121 QVSTDEVYGTLGATGLFTEETPLTPNSPYSASKAGGDLLVRAYHETFGLPVNITRCSNNY 180 Q+STDEVYGTLG TG FTEETPL PNSPYSASKA DLLVRAYHET+GLPVNITRCSNNY Sbjct: 121 QISTDEVYGTLGETGYFTEETPLAPNSPYSASKASADLLVRAYHETYGLPVNITRCSNNY 180 Query: 181 GPYQFPEKLIPLMISRALADQALPVYGDGMNIRDWLYVEDHCSAIDLVIHEGVNGEVYNI 240 GPY FPEKLIPLMI AL D+ LPVYGDG+NIRDWLYVEDHC+AIDLVIH+G +GEVYN+ Sbjct: 181 GPYHFPEKLIPLMIINALHDKELPVYGDGLNIRDWLYVEDHCAAIDLVIHKGKDGEVYNV 240 Query: 241 GGNNERTNVHIVKTVLQELGKPDSLITYVQDRPGHDRRYGIDPTKITRELGWKPKHTFET 300 GGNNERTN+ IVK +L+ELGK ++LI +V+DRPGHDRRY ID TK+ ELGW+PK+ FET Sbjct: 241 GGNNERTNIEIVKRILKELGKSENLIRFVKDRPGHDRRYAIDATKLKTELGWQPKYDFET 300 Query: 301 GIKETIQWYLNNQEWWTRIQSGEYQKYAELQYGSRL 336 GI +TIQWYL+N++WW RI SG+YQ Y E QY RL Sbjct: 301 GIVKTIQWYLDNRDWWERIISGDYQTYYEQQYKQRL 336 >Q7BG47.1 Q7BG47_BACST dTDP-glucose 4,6-dehydratase EC=4.2.1.46 Length = 342 Score = 540 bits (1390), Expect = e-151, Method: Compositional matrix adjust. Identities = 247/339 (72%), Positives = 291/339 (85%) Query: 1 MKLLVTGGAGFIGSNFVIYMLQQHPEYKIINVDALTYAGNLENLKSIENHPNYTFAKADI 60 MK+L+TGGAGFIGSNFV YM+Q++P Y+IIN+DALTYAGNLEN+K +EN PNY F K DI Sbjct: 3 MKVLITGGAGFIGSNFVNYMVQKYPSYQIINLDALTYAGNLENVKEVENEPNYRFVKGDI 62 Query: 61 TDVQAMDVLFSQGVDVVVNFAAESHVDRSILEPDVFVKTNVLGTQVLLDAAKKYNVTKFV 120 TD + ++ LF++G+D VVNFAAESHVDRSI +P +F+KTNV GTQVLLDAAKKY V K++ Sbjct: 63 TDRELIESLFAEGIDAVVNFAAESHVDRSIADPGIFIKTNVQGTQVLLDAAKKYGVKKYI 122 Query: 121 QVSTDEVYGTLGATGLFTEETPLTPNSPYSASKAGGDLLVRAYHETFGLPVNITRCSNNY 180 Q+STDEVYGTLG TG FTE TPL PNSPYSASKAG DLLVRAYHET+GLPVNITRCSNNY Sbjct: 123 QISTDEVYGTLGETGYFTETTPLAPNSPYSASKAGADLLVRAYHETYGLPVNITRCSNNY 182 Query: 181 GPYQFPEKLIPLMISRALADQALPVYGDGMNIRDWLYVEDHCSAIDLVIHEGVNGEVYNI 240 GPY FPEKLIPLMI AL +Q LPVYGDG+N+RDWL+VEDHCSAIDLV+H G NGEVYN+ Sbjct: 183 GPYHFPEKLIPLMIINALNNQPLPVYGDGLNVRDWLHVEDHCSAIDLVLHHGKNGEVYNV 242 Query: 241 GGNNERTNVHIVKTVLQELGKPDSLITYVQDRPGHDRRYGIDPTKITRELGWKPKHTFET 300 GGNNERTN+ +VK +L+ L KP+SLIT+V+DR GHDRRY ID TKI ELGW+PK+TFE Sbjct: 243 GGNNERTNIEVVKAILRYLNKPESLITFVEDRLGHDRRYAIDATKIREELGWQPKYTFEE 302 Query: 301 GIKETIQWYLNNQEWWTRIQSGEYQKYAELQYGSRLGDS 339 G+K+TI WYL N+ WW I SGEY++Y + QYG +LG S Sbjct: 303 GLKQTINWYLENRSWWENILSGEYKEYYKRQYGQKLGRS 341 >A2BD31.1 A2BD31_9BACI dTDP-glucose 4,6-dehydratase EC=4.2.1.46 Length = 339 Score = 535 bits (1377), Expect = e-150, Method: Compositional matrix adjust. Identities = 245/337 (72%), Positives = 288/337 (85%) Query: 1 MKLLVTGGAGFIGSNFVIYMLQQHPEYKIINVDALTYAGNLENLKSIENHPNYTFAKADI 60 MK+L+TGGAGFIGSNFV YM++++P Y+IIN+DALTYAGNLEN+K +EN PNY F K DI Sbjct: 1 MKVLITGGAGFIGSNFVNYMVRKYPSYQIINLDALTYAGNLENVKEVENEPNYRFVKGDI 60 Query: 61 TDVQAMDVLFSQGVDVVVNFAAESHVDRSILEPDVFVKTNVLGTQVLLDAAKKYNVTKFV 120 TD + ++ LF++G+D VVNFAAESHVDRSI +P VFV+TN+ GTQVLLDAAKKY V K++ Sbjct: 61 TDRELIESLFAEGIDAVVNFAAESHVDRSITDPGVFVRTNIQGTQVLLDAAKKYGVKKYI 120 Query: 121 QVSTDEVYGTLGATGLFTEETPLTPNSPYSASKAGGDLLVRAYHETFGLPVNITRCSNNY 180 Q+STDEVYGTLG TG FTE TPL PNSPYSASKA D LVRAYHET+GLPVNITRCSNNY Sbjct: 121 QISTDEVYGTLGETGYFTETTPLAPNSPYSASKASADFLVRAYHETYGLPVNITRCSNNY 180 Query: 181 GPYQFPEKLIPLMISRALADQALPVYGDGMNIRDWLYVEDHCSAIDLVIHEGVNGEVYNI 240 GPY FPEKLIPLMI AL DQ LPVYGDG+N+RDWL+VEDHCSAIDLV+H+G NGEVYNI Sbjct: 181 GPYHFPEKLIPLMIINALNDQPLPVYGDGLNVRDWLHVEDHCSAIDLVLHKGRNGEVYNI 240 Query: 241 GGNNERTNVHIVKTVLQELGKPDSLITYVQDRPGHDRRYGIDPTKITRELGWKPKHTFET 300 GGNNERTN+ +VK +L+ L KP+SLI +V+DR GHDRRY ID TKI +ELGWKPK+ FE Sbjct: 241 GGNNERTNIDLVKAILRYLNKPESLIIFVEDRLGHDRRYAIDATKIRKELGWKPKYAFEE 300 Query: 301 GIKETIQWYLNNQEWWTRIQSGEYQKYAELQYGSRLG 337 G+K+TI WYL N+ WW I SGEY++Y E QYG +LG Sbjct: 301 GLKKTIDWYLENRSWWENILSGEYKEYYERQYGQKLG 337 >B1D5Q1.1 B1D5Q1_9BACL dTDP-glucose 4,6-dehydratase EC=4.2.1.46 Length = 348 Score = 529 bits (1362), Expect = e-148, Method: Compositional matrix adjust. Identities = 241/336 (71%), Positives = 284/336 (84%) Query: 1 MKLLVTGGAGFIGSNFVIYMLQQHPEYKIINVDALTYAGNLENLKSIENHPNYTFAKADI 60 MKLL+TGGAGFIGSNFV YM+ ++P+Y+ INVDALTYAGN ENL+ +ENHP+YTF KADI Sbjct: 10 MKLLITGGAGFIGSNFVHYMMSRYPDYEFINVDALTYAGNPENLRQVENHPHYTFVKADI 69 Query: 61 TDVQAMDVLFSQGVDVVVNFAAESHVDRSILEPDVFVKTNVLGTQVLLDAAKKYNVTKFV 120 D A+ LF G+D V+NFAAESHVDRSIL+P +FV TN++GTQ LLD +K + V ++V Sbjct: 70 ADQAALTPLFESGIDAVINFAAESHVDRSILQPGLFVHTNIVGTQTLLDLSKTHQVKRYV 129 Query: 121 QVSTDEVYGTLGATGLFTEETPLTPNSPYSASKAGGDLLVRAYHETFGLPVNITRCSNNY 180 QVSTDEVYGTLGA GLFTE TPL PNSPYSASKAG DLLVRAYHET+GLP ITRCSNNY Sbjct: 130 QVSTDEVYGTLGAAGLFTENTPLAPNSPYSASKAGADLLVRAYHETYGLPAVITRCSNNY 189 Query: 181 GPYQFPEKLIPLMISRALADQALPVYGDGMNIRDWLYVEDHCSAIDLVIHEGVNGEVYNI 240 GPYQFPEKLIPLMI AL D+ LPVYGDG+ IRDWLYVEDHC AIDLV+H+G GEVYN+ Sbjct: 190 GPYQFPEKLIPLMILNALQDKPLPVYGDGLQIRDWLYVEDHCKAIDLVLHQGRIGEVYNV 249 Query: 241 GGNNERTNVHIVKTVLQELGKPDSLITYVQDRPGHDRRYGIDPTKITRELGWKPKHTFET 300 GG+NERTN+H+V+T+LQELGKP+SLI +V+DRPGHDRRY ID KI ELGW P+H+FE Sbjct: 250 GGSNERTNLHVVRTILQELGKPESLIRHVEDRPGHDRRYAIDADKIKSELGWTPEHSFEN 309 Query: 301 GIKETIQWYLNNQEWWTRIQSGEYQKYAELQYGSRL 336 GIK TI+WYL+N+EW ++ SG Y+KY + QY RL Sbjct: 310 GIKSTIEWYLHNEEWLEQVVSGAYRKYYDTQYAKRL 345 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.136 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,128,638,048 Number of extensions: 90555154 Number of successful extensions: 263706 Number of sequences better than 1.0e-05: 9074 Number of HSP's gapped: 245723 Number of HSP's successfully gapped: 9114 Length of query: 340 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 204 Effective length of database: 1,779,438,163 Effective search space: 363005385252 Effective search space used: 363005385252 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)