BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0554.0015.p (280 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B0TC87.1 B0TC87_HELMI Cobalt import ATP-binding protein cbio 2 232 3e-59 Q2RFS8.1 CBIO_MOOTA Cobalt import ATP-binding protein cbiO EC=3.... 231 5e-59 B8D0T9.1 B8D0T9_HALOH ABC transporter related 228 4e-58 Q8R7Y4.2 CBIO2_THETN Cobalt import ATP-binding protein cbiO 2 EC... 223 1e-56 A7GK49.1 A7GK49_BACCN ABC transporter-related protein 221 5e-56 >B0TC87.1 B0TC87_HELMI Cobalt import ATP-binding protein cbio 2 Length = 284 Score = 232 bits (591), Expect = 3e-59, Method: Compositional matrix adjust. Identities = 125/279 (44%), Positives = 179/279 (64%), Gaps = 6/279 (2%) Query: 1 MIRIQDVTFYYNEPDEASGHAGLRPALSKVSLDIQPGEFVALLGRNGSGKSSLSRLLNGI 60 +I +++V+F Y PD + A LS VS+ I+ GE+VA+LG NGSGKS+L++ LN + Sbjct: 6 LISVENVSFQYRNPDGDAIQA-----LSNVSITIRSGEWVAVLGANGSGKSTLAKHLNAL 60 Query: 61 ELPTEGTVTVDGRRTAEPHEVQEIRRAVQIVFQNPENQQVGLTVGEDIAFGLSNIGWPQQ 120 P EG V VDG T + + +IRR V +VFQNP+NQ V TV ED+AFG N+G P + Sbjct: 61 LTPAEGRVLVDGLDTKDARQRYDIRRRVGMVFQNPDNQLVATTVEEDVAFGPENLGLPSE 120 Query: 121 EIRSRIAWALQLVGLEADEERPVTRLSGGEKQKLALASVLALAPRYLILDEATSMLDPVA 180 EIR R+ AL +VGL++ ERP LSGG+KQ++A+A LAL P YL+ DEATSMLDP + Sbjct: 121 EIRRRVDAALAVVGLQSLRERPPHDLSGGQKQRVAIAGALALQPAYLVFDEATSMLDPQS 180 Query: 181 RRRFVETLHEVRQRVPFTLIYITHHLEEVMGADRWVLFREGRIVTSGPPSGLQKDTQLLH 240 RR ++T+ + + + T+I ITH ++E A+R V+ +GR+V G P L ++ LL Sbjct: 181 RREVLQTVRRLHKELAMTVITITHDMDEAAEAERLVVMSQGRVVFDGLPESLFREESLLE 240 Query: 241 DCGLELPYYYKLAELLRRSGIPAP-ATLSEQELRGLLCR 278 GLE P ++A LR G+ P ++ ++L LCR Sbjct: 241 QAGLEPPPSAEIARRLRERGLTIPLEAVTMEKLVNALCR 279 >Q2RFS8.1 CBIO_MOOTA Cobalt import ATP-binding protein cbiO EC=3.6.3.- Length = 276 Score = 231 bits (589), Expect = 5e-59, Method: Compositional matrix adjust. Identities = 128/261 (49%), Positives = 164/261 (62%), Gaps = 8/261 (3%) Query: 1 MIRIQDVTFYYNEPDEASGHAGLRPALSKVSLDIQPGEFVALLGRNGSGKSSLSRLLNGI 60 MI IQ T+ Y PAL K+ L I+PGEF+A+ G NGSGKS+L+RLLNG+ Sbjct: 1 MIAIQGATYIY--------PGSTVPALEKIDLAIEPGEFLAITGPNGSGKSTLARLLNGL 52 Query: 61 ELPTEGTVTVDGRRTAEPHEVQEIRRAVQIVFQNPENQQVGLTVGEDIAFGLSNIGWPQQ 120 P +G V VDG T + + IRR V +VFQ+P+NQ V TV ED+AFGL N+G P Sbjct: 53 LRPRQGRVLVDGMDTGDDTALITIRRRVGMVFQDPDNQLVAATVAEDVAFGLENLGLPPA 112 Query: 121 EIRSRIAWALQLVGLEADEERPVTRLSGGEKQKLALASVLALAPRYLILDEATSMLDPVA 180 EI R+ AL+ VGLE RP LSGG+KQ+LALA VLA+ PRYL+LDEAT+MLDPVA Sbjct: 113 EIEKRVDLALRSVGLEDLAARPPHLLSGGQKQRLALAGVLAMEPRYLVLDEATAMLDPVA 172 Query: 181 RRRFVETLHEVRQRVPFTLIYITHHLEEVMGADRWVLFREGRIVTSGPPSGLQKDTQLLH 240 R +E + +RQ +I ITH +EE + ADR ++ + GRI G P L ++L Sbjct: 173 REEILEQVLRLRQEQGIAIILITHLMEEAVRADRMLIMKSGRIFWQGSPRELSYQERILA 232 Query: 241 DCGLELPYYYKLAELLRRSGI 261 GL LP LA+ LR+ GI Sbjct: 233 AAGLRLPAVIALAKSLRQGGI 253 >B8D0T9.1 B8D0T9_HALOH ABC transporter related Length = 273 Score = 228 bits (582), Expect = 4e-58, Method: Compositional matrix adjust. Identities = 111/253 (43%), Positives = 167/253 (66%) Query: 25 PALSKVSLDIQPGEFVALLGRNGSGKSSLSRLLNGIELPTEGTVTVDGRRTAEPHEVQEI 84 PAL + L ++ GEFVA++G NGSGKS+L+RLLN + +PTEG + +DG +T+E + EI Sbjct: 21 PALKNIDLTVESGEFVAIIGSNGSGKSTLARLLNVLLVPTEGEILIDGLKTSEKENIWEI 80 Query: 85 RRAVQIVFQNPENQQVGLTVGEDIAFGLSNIGWPQQEIRSRIAWALQLVGLEADEERPVT 144 R+ V IVFQNP+NQ V TV D+AFGL N+G P +EIR R+ AL++VG+ +E Sbjct: 81 RQKVGIVFQNPDNQLVATTVENDVAFGLENLGVPSREIRLRVDNALKMVGMNGFQEHETH 140 Query: 145 RLSGGEKQKLALASVLALAPRYLILDEATSMLDPVARRRFVETLHEVRQRVPFTLIYITH 204 +LSGG+KQ++A+A V+A+ ++LDE T+MLDP R+ ++T+ + + T+I+ITH Sbjct: 141 KLSGGQKQRVAIAGVIAMESDCIVLDEPTAMLDPRGRKEVMDTVTYLNKEKGITIIHITH 200 Query: 205 HLEEVMGADRWVLFREGRIVTSGPPSGLQKDTQLLHDCGLELPYYYKLAELLRRSGIPAP 264 +EE AD+ + +G+I SG P + +D + L L++P +LA LR++G+ P Sbjct: 201 FMEEAALADKIYVMHQGQIYKSGTPRDIFQDVEGLKSVNLDVPQVVELANNLRKAGMTVP 260 Query: 265 ATLSEQELRGLLC 277 LS EL LC Sbjct: 261 RILSIDELVSCLC 273 >Q8R7Y4.2 CBIO2_THETN Cobalt import ATP-binding protein cbiO 2 EC=3.6.3.- Length = 277 Score = 223 bits (568), Expect = 1e-56, Method: Compositional matrix adjust. Identities = 109/279 (39%), Positives = 178/279 (63%), Gaps = 6/279 (2%) Query: 1 MIRIQDVTFYYNEPDEASGHAGLRPALSKVSLDIQPGEFVALLGRNGSGKSSLSRLLNGI 60 +IR Q+V+F Y+E G + P L ++L + G+F+ ++G NGSGKS+L++ N + Sbjct: 4 IIRAQNVSFCYSE-----GESKSPPVLKDINLQFEKGQFIGIIGHNGSGKSTLAKHFNAL 58 Query: 61 ELPTEGTVTVDGRRTAEPHEVQEIRRAVQIVFQNPENQQVGLTVGEDIAFGLSNIGWPQQ 120 LPT+G V V T + + +IR+ +VFQNP+NQ V V ED+AFG N+G P + Sbjct: 59 LLPTKGNVYVKDMDTKDAKHLWDIRQTAGLVFQNPDNQIVAAIVEEDVAFGPENLGIPPE 118 Query: 121 EIRSRIAWALQLVGLEADEERPVTRLSGGEKQKLALASVLALAPRYLILDEATSMLDPVA 180 EIR R+ +AL+ VG+ ++ P LSGG+KQ++A+A ++A+ P ++LDE T+MLDP+ Sbjct: 119 EIRKRVEYALKAVGMWEYKDFPPHMLSGGQKQRVAIAGIIAMKPECIVLDEPTAMLDPIG 178 Query: 181 RRRFVETLHEVRQRVPFTLIYITHHLEEVMGADRWVLFREGRIVTSGPPSGLQKDTQLLH 240 RR + T+ ++ + T+I ITH +EEV+ ADR ++ +G++V G P + K+ ++L Sbjct: 179 RREVISTIKKLNKEEGITVILITHFMEEVVDADRVIVMDDGKVVLDGTPKEVFKEVEVLK 238 Query: 241 DCGLELPYYYKLAELLRRSGIPAPA-TLSEQELRGLLCR 278 GL++P +LA LR+ GI P+ L+ +E+ +CR Sbjct: 239 KIGLDVPQVTELAHQLRKEGIDIPSDILTIEEMVEFICR 277 >A7GK49.1 A7GK49_BACCN ABC transporter-related protein Length = 280 Score = 221 bits (563), Expect = 5e-56, Method: Compositional matrix adjust. Identities = 116/265 (43%), Positives = 170/265 (64%), Gaps = 8/265 (3%) Query: 2 IRIQDVTFYYNEPDEASGHAGLRPALSKVSLDIQPGEFVALLGRNGSGKSSLSRLLNGIE 61 +R+Q+V+F Y P + AL VS + GE+V+++G+NGSGKS+L++LLNG+ Sbjct: 6 LRVQNVSFQY--PGATA------YALKDVSFSLNEGEWVSVIGQNGSGKSTLAKLLNGLF 57 Query: 62 LPTEGTVTVDGRRTAEPHEVQEIRRAVQIVFQNPENQQVGLTVGEDIAFGLSNIGWPQQE 121 LP GT+TV+G V +IR+ + +VFQNP+NQ VG TV +D+ FGL NIG P+++ Sbjct: 58 LPESGTITVNGTMALSEETVWDIRKQIGMVFQNPDNQFVGTTVQDDVVFGLENIGMPREQ 117 Query: 122 IRSRIAWALQLVGLEADEERPVTRLSGGEKQKLALASVLALAPRYLILDEATSMLDPVAR 181 + R+ AL LV +E LSGG+KQ++A+A +LAL P LILDEATSMLDP R Sbjct: 118 MVERMKQALHLVRMEEFLHEEPHSLSGGQKQRVAIAGILALQPSILILDEATSMLDPKGR 177 Query: 182 RRFVETLHEVRQRVPFTLIYITHHLEEVMGADRWVLFREGRIVTSGPPSGLQKDTQLLHD 241 VET+ ++ T++ ITH LEE +DR ++ +G I+ G P + K + +L + Sbjct: 178 VEVVETVRQLVDEKGITVLSITHDLEEAAQSDRIIVLNKGEILEEGTPQKIFKSSHMLKE 237 Query: 242 CGLELPYYYKLAELLRRSGIPAPAT 266 GL++P+ K+AELL+RS IP T Sbjct: 238 IGLDVPFSVKIAELLKRSEIPLQNT 262 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.137 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,663,047,258 Number of extensions: 69317486 Number of successful extensions: 474930 Number of sequences better than 1.0e-05: 90540 Number of HSP's gapped: 369659 Number of HSP's successfully gapped: 98236 Length of query: 280 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 146 Effective length of database: 1,798,280,193 Effective search space: 262548908178 Effective search space used: 262548908178 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)