BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0554.0009.p (433 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q59298.1 Q59298_9CLOT Dihydrolipoamide acetyltransferase EC=2.3.... 431 e-119 B1ILQ4.1 B1ILQ4_CLOBK TPP-dependent acetoin dehydrogenase comple... 427 e-118 C1FN99.1 C1FN99_CLOBJ TPP-dependent acetoin dehydrogenase comple... 427 e-117 B1QD81.1 B1QD81_CLOBO TPP-dependent acetoin dehydrogenase comple... 426 e-117 A7FUD9.1 A7FUD9_CLOB1 TPP-dependent acetoin dehydrogenase comple... 423 e-116 >Q59298.1 Q59298_9CLOT Dihydrolipoamide acetyltransferase EC=2.3.1.12 Length = 443 Score = 431 bits (1109), Expect = e-119, Method: Compositional matrix adjust. Identities = 224/436 (51%), Positives = 286/436 (65%), Gaps = 9/436 (2%) Query: 1 MTEGTISNWRKAEGDTVTQGEILFDVETDKISNEVEAKLGGVLRKIIVEEG-TVGIFQPV 59 MTEGT+ W+KAEGD V GEI F+V TDK++NEVEA G++RK++V EG TV +PV Sbjct: 14 MTEGTLVTWKKAEGDQVKVGEIFFEVSTDKLTNEVEASDEGIVRKLLVNEGDTVECLKPV 73 Query: 60 AVIGDANEDISALLXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRIIASPYAKKTA 119 A+IG A+EDIS+LL ++ ASP AKK A Sbjct: 74 AIIGSADEDISSLLNGSSEGSESAEQNDTKAPKKEAEAPKGAVEKQQGKVKASPAAKKLA 133 Query: 120 ALLSVELQQVRGSGPQGRITAGDVRKHAAEGGSGXXXXXXXXXXXXXXXXXLGIDPSGLH 179 A ++++ V G+GPQGRIT DV K+ + + L +D S + Sbjct: 134 AENNIDITLVEGTGPQGRITTEDVEKYIEDSKNASKASPMASKVAAE----LNVDLSTIE 189 Query: 180 KEGRIMKEDIRSFAA--ASTKTTAQPAVQAAIQEVTRVPMNSMRRIIAKRMLESQAVSPA 237 K+GRIMKED+ S A + P+ +++ VPM +R+II+ RM ES SP Sbjct: 190 KDGRIMKEDVLSLCKGNAPEECKVNPSEDKYTEKI--VPMTQIRKIISARMHESWITSPT 247 Query: 238 VTYNMKLDTTALGLLRQQLKDTLKVTYTDLLVSIAAKVLLEFPLLNCSVEGTELVLRNYA 297 VTY++K+D T+L + LKD KVTYTDL+V I +KVLL+FPLLNCS+ G EL+ RNY Sbjct: 248 VTYDIKVDMTSLKRFKDALKDVCKVTYTDLIVKIVSKVLLQFPLLNCSINGNELITRNYV 307 Query: 298 NIGVAVGLEEGLIVPVVKNAAQKGLAEISQEVKRLAAGARNNSLTPEELTGGTFTITNLG 357 N+GVAV ++ GL+VPVVK A +KGL EIS EVK LA A++N L PE +TGGTFTITNLG Sbjct: 308 NMGVAVAIDGGLVVPVVKYANEKGLKEISTEVKDLAKKAKSNQLKPENMTGGTFTITNLG 367 Query: 358 MYGMESFSPIINQPEVAILGVNAIVDTPVAVNGEIVIRPLMNLSLTADHRAVDGAVAAQF 417 M+G+E FSPIINQPEVAILGVN I +TPV NGEIVI+PLMNLSLTA+HRAVDG+VAAQF Sbjct: 368 MFGIEYFSPIINQPEVAILGVNKITETPVVQNGEIVIKPLMNLSLTANHRAVDGSVAAQF 427 Query: 418 MQKIKAYAENPALLLL 433 + K+K Y E P LL+L Sbjct: 428 LSKVKEYMEKPELLML 443 >B1ILQ4.1 B1ILQ4_CLOBK TPP-dependent acetoin dehydrogenase complex, E2 component, dihydrolipoamide acetyltransferase EC=2.3.1.12 Length = 436 Score = 427 bits (1099), Expect = e-118, Method: Compositional matrix adjust. Identities = 226/434 (52%), Positives = 286/434 (65%), Gaps = 12/434 (2%) Query: 1 MTEGTISNWRKAEGDTVTQGEILFDVETDKISNEVEAKLGGVLRKIIVEEGTV-GIFQPV 59 MTEG + W K EGDT+ GE LFDV TDK++N VEAK G++RKI+VEEGTV +PV Sbjct: 14 MTEGELVKWHKKEGDTIKVGETLFDVTTDKLTNNVEAKADGIVRKILVEEGTVVECLKPV 73 Query: 60 AVIGDANEDISALLXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRIIASPYAKKTA 119 A+IGD +EDIS LL R SP AK+ A Sbjct: 74 AIIGDKDEDISNLLKESLQDSKENEVEKEVKESKEEIKDNRKIKKGE-RTKISPIAKRLA 132 Query: 120 ALLSVELQQVRGSGPQGRITAGDVRKHAAEGGSGXXXXXXXXXXXXXXXXXLGIDPSGLH 179 V++Q + G+GP+GRI DV + + LG++ + Sbjct: 133 KENDVDIQLLDGTGPEGRIVLKDVESYIENNKNNIKTSPVAEKMAKD----LGVNLEDIK 188 Query: 180 KEGRIMKEDIRSFAAASTKTTAQPAVQAAIQEVTRVPMNSMRRIIAKRMLESQAVSPAVT 239 K+GRIMK+DI F ST P+V + + RV M++MR++IA RM ES +SP VT Sbjct: 189 KDGRIMKDDILEFIQKST-----PSVGEDLMD-RRVKMSTMRKVIASRMSESSKISPTVT 242 Query: 240 YNMKLDTTALGLLRQQLKDTLKVTYTDLLVSIAAKVLLEFPLLNCSVEGTELVLRNYANI 299 Y++++D T L L++Q+KD KVTYTDLLV I +KVL+++PL+NCS+EG E++ RNY+NI Sbjct: 243 YDIEVDMTNLKRLKEQIKDEWKVTYTDLLVKIVSKVLIQYPLVNCSIEGDEMIFRNYSNI 302 Query: 300 GVAVGLEEGLIVPVVKNAAQKGLAEISQEVKRLAAGARNNSLTPEELTGGTFTITNLGMY 359 GVAV LEEGL+VPVVK A QKGL +IS EVK LA A+NN LT E TGGTFTITNLGM+ Sbjct: 303 GVAVALEEGLVVPVVKYANQKGLKDISIEVKELAHKAKNNGLTEENSTGGTFTITNLGMF 362 Query: 360 GMESFSPIINQPEVAILGVNAIVDTPVAVNGEIVIRPLMNLSLTADHRAVDGAVAAQFMQ 419 G++SFSPIINQPEVAILGVN I +TPV NGEIVI+PLM+LSLTADHRAVDGAVAAQF++ Sbjct: 363 GIKSFSPIINQPEVAILGVNMITNTPVVENGEIVIKPLMSLSLTADHRAVDGAVAAQFLK 422 Query: 420 KIKAYAENPALLLL 433 +K Y E P LL+L Sbjct: 423 AVKKYMEKPELLIL 436 >C1FN99.1 C1FN99_CLOBJ TPP-dependent acetoin dehydrogenase complex, E2 component, dihydrolipoyllysine-residue acetyltransferase EC=2.3.1.12 Length = 436 Score = 427 bits (1097), Expect = e-117, Method: Compositional matrix adjust. Identities = 225/434 (51%), Positives = 286/434 (65%), Gaps = 12/434 (2%) Query: 1 MTEGTISNWRKAEGDTVTQGEILFDVETDKISNEVEAKLGGVLRKIIVEEGTV-GIFQPV 59 MTEG + W K EGDT+ GE LFDV TDK++N VEAK G++RKI+V+EGTV +PV Sbjct: 14 MTEGELVKWHKKEGDTIKVGETLFDVTTDKLTNNVEAKADGIVRKILVDEGTVVECLKPV 73 Query: 60 AVIGDANEDISALLXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRIIASPYAKKTA 119 A+IGD +EDIS LL R SP AK+ A Sbjct: 74 AIIGDKDEDISNLLKESLQDSKENEVEKEVKESKEEIKDNRKIKKGE-RTKISPIAKRLA 132 Query: 120 ALLSVELQQVRGSGPQGRITAGDVRKHAAEGGSGXXXXXXXXXXXXXXXXXLGIDPSGLH 179 V++Q + G+GP+GRI DV + + LG++ + Sbjct: 133 KENDVDIQLLDGTGPEGRIVLKDVESYIENNKNNIKTSPVAEKMAKD----LGVNLEDIK 188 Query: 180 KEGRIMKEDIRSFAAASTKTTAQPAVQAAIQEVTRVPMNSMRRIIAKRMLESQAVSPAVT 239 K+GRIMK+DI F ST P+V + + RV M++MR++IA RM ES +SP VT Sbjct: 189 KDGRIMKDDILEFIQKST-----PSVGEDLMD-RRVKMSTMRKVIASRMSESSKISPTVT 242 Query: 240 YNMKLDTTALGLLRQQLKDTLKVTYTDLLVSIAAKVLLEFPLLNCSVEGTELVLRNYANI 299 Y++++D T L L++Q+KD KVTYTDLLV I +KVL+++PL+NCS+EG E++ RNY+NI Sbjct: 243 YDIEVDMTNLKRLKEQIKDEWKVTYTDLLVKIVSKVLIQYPLVNCSIEGDEMIFRNYSNI 302 Query: 300 GVAVGLEEGLIVPVVKNAAQKGLAEISQEVKRLAAGARNNSLTPEELTGGTFTITNLGMY 359 GVAV LEEGL+VPVVK A QKGL +IS EVK LA A+NN LT E TGGTFTITNLGM+ Sbjct: 303 GVAVALEEGLVVPVVKYANQKGLKDISIEVKELAHKAKNNGLTEENSTGGTFTITNLGMF 362 Query: 360 GMESFSPIINQPEVAILGVNAIVDTPVAVNGEIVIRPLMNLSLTADHRAVDGAVAAQFMQ 419 G++SFSPIINQPEVAILGVN I +TPV NGEIVI+PLM+LSLTADHRAVDGAVAAQF++ Sbjct: 363 GIKSFSPIINQPEVAILGVNMITNTPVVENGEIVIKPLMSLSLTADHRAVDGAVAAQFLK 422 Query: 420 KIKAYAENPALLLL 433 +K Y E P LL+L Sbjct: 423 AVKKYMEKPELLIL 436 >B1QD81.1 B1QD81_CLOBO TPP-dependent acetoin dehydrogenase complex, E2 component, dihydrolipoamide acetyltransferase EC=2.3.1.12 Length = 436 Score = 426 bits (1095), Expect = e-117, Method: Compositional matrix adjust. Identities = 225/434 (51%), Positives = 286/434 (65%), Gaps = 12/434 (2%) Query: 1 MTEGTISNWRKAEGDTVTQGEILFDVETDKISNEVEAKLGGVLRKIIVEEGTV-GIFQPV 59 MTEG + W K EGDT+ GE LFDV TDK++N VEAK G++RKI+V+EGTV +PV Sbjct: 14 MTEGELVKWHKKEGDTIKVGETLFDVTTDKLTNNVEAKADGIVRKILVDEGTVVECLKPV 73 Query: 60 AVIGDANEDISALLXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRIIASPYAKKTA 119 A+IGD +EDIS LL R SP AK+ A Sbjct: 74 AIIGDKDEDISNLLKESLQDSKENEVEKEVKESKEEIKDNRKIKKGE-RTKISPIAKRLA 132 Query: 120 ALLSVELQQVRGSGPQGRITAGDVRKHAAEGGSGXXXXXXXXXXXXXXXXXLGIDPSGLH 179 V++Q + G+GP+GRI DV + + LG++ G+ Sbjct: 133 KENDVDIQLLDGTGPEGRIVLKDVESYIENNKNNIKTSPVAEKMAKD----LGVNLEGIK 188 Query: 180 KEGRIMKEDIRSFAAASTKTTAQPAVQAAIQEVTRVPMNSMRRIIAKRMLESQAVSPAVT 239 K+GRIMK+DI F S P+V + + RV M++MR++IA RM ES +SP VT Sbjct: 189 KDGRIMKDDILEFIQKSI-----PSVGEDLMD-RRVKMSTMRKVIASRMSESSKISPTVT 242 Query: 240 YNMKLDTTALGLLRQQLKDTLKVTYTDLLVSIAAKVLLEFPLLNCSVEGTELVLRNYANI 299 Y++++D T L L++Q+KD KVTYTDLLV I +KVL+++PL+NCS+EG E++ RNY+NI Sbjct: 243 YDIEVDMTNLKRLKEQIKDEWKVTYTDLLVKIVSKVLIQYPLVNCSIEGDEMIFRNYSNI 302 Query: 300 GVAVGLEEGLIVPVVKNAAQKGLAEISQEVKRLAAGARNNSLTPEELTGGTFTITNLGMY 359 GVAV LEEGL+VPVVK A QKGL +IS EVK LA A+NN LT E TGGTFTITNLGM+ Sbjct: 303 GVAVALEEGLVVPVVKYANQKGLKDISIEVKELAHKAKNNGLTEENSTGGTFTITNLGMF 362 Query: 360 GMESFSPIINQPEVAILGVNAIVDTPVAVNGEIVIRPLMNLSLTADHRAVDGAVAAQFMQ 419 G++SFSPIINQPEVAILGVN I +TPV NGEIVI+PLM+LSLTADHRAVDGAVAAQF++ Sbjct: 363 GIKSFSPIINQPEVAILGVNMITNTPVVENGEIVIKPLMSLSLTADHRAVDGAVAAQFLK 422 Query: 420 KIKAYAENPALLLL 433 +K Y E P LL+L Sbjct: 423 AVKKYMEKPELLIL 436 >A7FUD9.1 A7FUD9_CLOB1 TPP-dependent acetoin dehydrogenase complex, E2 component, dihydrolipoamide acetyltransferase EC=2.3.1.12 Length = 436 Score = 423 bits (1088), Expect = e-116, Method: Compositional matrix adjust. Identities = 224/434 (51%), Positives = 286/434 (65%), Gaps = 12/434 (2%) Query: 1 MTEGTISNWRKAEGDTVTQGEILFDVETDKISNEVEAKLGGVLRKIIVEEGTV-GIFQPV 59 MTEG + W K EGDT+ GE LFDV TDK++N VEAK G++RKI+V+EGTV +PV Sbjct: 14 MTEGELVKWHKKEGDTIKVGETLFDVTTDKLTNNVEAKADGIVRKILVDEGTVVECLKPV 73 Query: 60 AVIGDANEDISALLXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRIIASPYAKKTA 119 A+IGD +EDIS LL R SP AK+ A Sbjct: 74 AIIGDKDEDISNLLRESLQDSKGNEVEKEVKESKEEIKDNRKIKKGE-RTKISPIAKRLA 132 Query: 120 ALLSVELQQVRGSGPQGRITAGDVRKHAAEGGSGXXXXXXXXXXXXXXXXXLGIDPSGLH 179 V++Q + G+GP+GRI DV + + LG++ G+ Sbjct: 133 KENDVDIQLLDGTGPEGRIVLKDVESYIENNKNNIKTSPVAEKIAKD----LGVNLEGIK 188 Query: 180 KEGRIMKEDIRSFAAASTKTTAQPAVQAAIQEVTRVPMNSMRRIIAKRMLESQAVSPAVT 239 K+GRIMK+DI F S P+V + + RV M++MR++IA RM ES +SP VT Sbjct: 189 KDGRIMKDDILEFIQKSI-----PSVGEDLMD-RRVKMSTMRKVIASRMSESSKISPTVT 242 Query: 240 YNMKLDTTALGLLRQQLKDTLKVTYTDLLVSIAAKVLLEFPLLNCSVEGTELVLRNYANI 299 Y++++D T L L++Q+K+ KVTYTDLLV I +KVL+++PL+NCS+EG E++ RNY+NI Sbjct: 243 YDIEVDMTNLKRLKEQIKNEWKVTYTDLLVKIVSKVLIQYPLVNCSIEGDEMIFRNYSNI 302 Query: 300 GVAVGLEEGLIVPVVKNAAQKGLAEISQEVKRLAAGARNNSLTPEELTGGTFTITNLGMY 359 GVAV LEEGL+VPVVK A QKGL +IS EVK LA A+NN LT E TGGTFTITNLGM+ Sbjct: 303 GVAVALEEGLVVPVVKYANQKGLKDISIEVKELAHKAKNNGLTEENSTGGTFTITNLGMF 362 Query: 360 GMESFSPIINQPEVAILGVNAIVDTPVAVNGEIVIRPLMNLSLTADHRAVDGAVAAQFMQ 419 G++SFSPIINQPEVAILGVN I +TPV NGEIVI+PLM+LSLTADHRAVDGAVAAQF++ Sbjct: 363 GIKSFSPIINQPEVAILGVNMITNTPVVENGEIVIKPLMSLSLTADHRAVDGAVAAQFLK 422 Query: 420 KIKAYAENPALLLL 433 +K Y E P LL+L Sbjct: 423 AVKKYMEKPELLIL 436 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.315 0.132 0.357 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,043,340,562 Number of extensions: 74048794 Number of successful extensions: 216708 Number of sequences better than 1.0e-05: 3851 Number of HSP's gapped: 211171 Number of HSP's successfully gapped: 4374 Length of query: 433 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 294 Effective length of database: 1,751,175,118 Effective search space: 514845484692 Effective search space used: 514845484692 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 133 (55.8 bits)