BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0554.0008.p (323 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B9E3H5.1 B9E3H5_CLOK1 Putative uncharacterized protein 432 e-119 A5MZI6.1 A5MZI6_CLOK5 PdhB EC=1.2.4.1 432 e-119 C1FN97.1 C1FN97_CLOBJ TPP-dependent acetoin dehydrogenase comple... 424 e-117 B1L1X9.1 B1L1X9_CLOBM TPP-dependent acetoin dehydrogenase comple... 422 e-116 C3KW36.1 C3KW36_CLOB6 TPP-dependent acetoin dehydrogenase comple... 422 e-116 >B9E3H5.1 B9E3H5_CLOK1 Putative uncharacterized protein Length = 337 Score = 432 bits (1111), Expect = e-119, Method: Compositional matrix adjust. Identities = 199/323 (61%), Positives = 255/323 (78%) Query: 1 MRKMTYGEGIREGMRSKMLEDPNVVLLGEDVGPYGGTFGVTKGLWEEFGEERVRDTPISE 60 M+K+TY + I+E M KM ED +V+L GEDVGP+GG FGV++G+ +EFGE RVRDTPISE Sbjct: 15 MKKITYSQAIKEAMSVKMREDKSVLLFGEDVGPFGGCFGVSQGMHDEFGEMRVRDTPISE 74 Query: 61 XXXXXXXXXXXXXXLRPVAELMFIDFLTFGMDGLVNQAAKMRYMFGGKISVPLVLRLPAG 120 L+P+AELMFIDFLT GMD LVNQAAKMRYMFGGKI +P+V+R+P G Sbjct: 75 GAIIGCAIGAAATGLKPIAELMFIDFLTVGMDMLVNQAAKMRYMFGGKIKLPMVVRVPCG 134 Query: 121 GGISAGAQHSGSLEAWVTHVPGLKVVYPSNAQDAWGMMLTAIEDDNPVVYIESKVLYSKK 180 G A AQHS SLEAWVTHVPGLKVVYPS AQDA+G+MLTAI+D+NPV++IE K+LY+ K Sbjct: 135 AGTQAAAQHSQSLEAWVTHVPGLKVVYPSTAQDAYGLMLTAIDDENPVIFIEHKILYAMK 194 Query: 181 FEVDDTVAAVPFGVASVKREGSDVSIITYGKQVDDALNAAKVLAEEGIEAEVIDLRSLYP 240 E ++ +PFG+A +KREG D +I+ G+ V +AL AA L++EGIE E+ID R+LYP Sbjct: 195 GEFEENSKPIPFGLADIKREGKDATIVATGRMVHEALAAADKLSKEGIEVEIIDPRTLYP 254 Query: 241 LDKEAIFSSVAKTHRALVVTEEVKRGGYGGELSAMISELCFDELDAPVVRIGALDTPVPY 300 DK+ +F+S+ KT+RA+VVTEE KRGGYGGE+SA+ISE FD LDAPVVRIG+LD P+P+ Sbjct: 255 FDKDTVFNSIKKTNRAVVVTEETKRGGYGGEISAVISEEVFDYLDAPVVRIGSLDVPIPF 314 Query: 301 SPALEAYVLPNADDIIQGIKSMF 323 +P LE+YV+PN+D I+ +K +F Sbjct: 315 TPKLESYVIPNSDKIVNAVKKLF 337 >A5MZI6.1 A5MZI6_CLOK5 PdhB EC=1.2.4.1 Length = 323 Score = 432 bits (1110), Expect = e-119, Method: Compositional matrix adjust. Identities = 199/323 (61%), Positives = 255/323 (78%) Query: 1 MRKMTYGEGIREGMRSKMLEDPNVVLLGEDVGPYGGTFGVTKGLWEEFGEERVRDTPISE 60 M+K+TY + I+E M KM ED +V+L GEDVGP+GG FGV++G+ +EFGE RVRDTPISE Sbjct: 1 MKKITYSQAIKEAMSVKMREDKSVLLFGEDVGPFGGCFGVSQGMHDEFGEMRVRDTPISE 60 Query: 61 XXXXXXXXXXXXXXLRPVAELMFIDFLTFGMDGLVNQAAKMRYMFGGKISVPLVLRLPAG 120 L+P+AELMFIDFLT GMD LVNQAAKMRYMFGGKI +P+V+R+P G Sbjct: 61 GAIIGCAIGAAATGLKPIAELMFIDFLTVGMDMLVNQAAKMRYMFGGKIKLPMVVRVPCG 120 Query: 121 GGISAGAQHSGSLEAWVTHVPGLKVVYPSNAQDAWGMMLTAIEDDNPVVYIESKVLYSKK 180 G A AQHS SLEAWVTHVPGLKVVYPS AQDA+G+MLTAI+D+NPV++IE K+LY+ K Sbjct: 121 AGTQAAAQHSQSLEAWVTHVPGLKVVYPSTAQDAYGLMLTAIDDENPVIFIEHKILYAMK 180 Query: 181 FEVDDTVAAVPFGVASVKREGSDVSIITYGKQVDDALNAAKVLAEEGIEAEVIDLRSLYP 240 E ++ +PFG+A +KREG D +I+ G+ V +AL AA L++EGIE E+ID R+LYP Sbjct: 181 GEFEENSKPIPFGLADIKREGKDATIVATGRMVHEALAAADKLSKEGIEVEIIDPRTLYP 240 Query: 241 LDKEAIFSSVAKTHRALVVTEEVKRGGYGGELSAMISELCFDELDAPVVRIGALDTPVPY 300 DK+ +F+S+ KT+RA+VVTEE KRGGYGGE+SA+ISE FD LDAPVVRIG+LD P+P+ Sbjct: 241 FDKDTVFNSIKKTNRAVVVTEETKRGGYGGEISAVISEEVFDYLDAPVVRIGSLDVPIPF 300 Query: 301 SPALEAYVLPNADDIIQGIKSMF 323 +P LE+YV+PN+D I+ +K +F Sbjct: 301 TPKLESYVIPNSDKIVNAVKKLF 323 >C1FN97.1 C1FN97_CLOBJ TPP-dependent acetoin dehydrogenase complex, E1 component, beta subunit EC=1.1.1.- Length = 323 Score = 424 bits (1091), Expect = e-117, Method: Compositional matrix adjust. Identities = 199/323 (61%), Positives = 254/323 (78%) Query: 1 MRKMTYGEGIREGMRSKMLEDPNVVLLGEDVGPYGGTFGVTKGLWEEFGEERVRDTPISE 60 M+K+TY E IRE MR+KM ED V++ GEDVG +GG FGVT L++EFGE+RVRDTPISE Sbjct: 1 MKKLTYAEAIREAMRTKMSEDDKVLIFGEDVGAFGGCFGVTGDLYKEFGEKRVRDTPISE 60 Query: 61 XXXXXXXXXXXXXXLRPVAELMFIDFLTFGMDGLVNQAAKMRYMFGGKISVPLVLRLPAG 120 LRP+AE+MF DFLT MD +VNQAAKMR+MFGGKIS+P+V+RLP G Sbjct: 61 GAIAGCAVGAAATGLRPIAEIMFGDFLTVSMDMIVNQAAKMRFMFGGKISLPMVVRLPEG 120 Query: 121 GGISAGAQHSGSLEAWVTHVPGLKVVYPSNAQDAWGMMLTAIEDDNPVVYIESKVLYSKK 180 G+ A AQHS SLEAW+THVPGLKVVYPS +QDA+G+M+ AIEDDNPV+++E K LY K Sbjct: 121 AGVQAAAQHSQSLEAWLTHVPGLKVVYPSTSQDAYGLMVAAIEDDNPVMFMEHKFLYGMK 180 Query: 181 FEVDDTVAAVPFGVASVKREGSDVSIITYGKQVDDALNAAKVLAEEGIEAEVIDLRSLYP 240 EV D + +P GVA +KREG DV+II GK V ++L AA +L+++GIE EVID R+LYP Sbjct: 181 GEVSDEIKRIPLGVADIKREGKDVTIIATGKMVHESLKAADILSKDGIEVEVIDPRTLYP 240 Query: 241 LDKEAIFSSVAKTHRALVVTEEVKRGGYGGELSAMISELCFDELDAPVVRIGALDTPVPY 300 LDKE IF+S+ KT+RA+VVTEE KRG Y GE+S++I+E FD LDAPV RIGAL+TP+P+ Sbjct: 241 LDKETIFNSIKKTNRAVVVTEENKRGAYSGEISSLINEEIFDYLDAPVGRIGALNTPIPF 300 Query: 301 SPALEAYVLPNADDIIQGIKSMF 323 SP LE+YV+P++ DI++ +K +F Sbjct: 301 SPTLESYVIPDSKDIVKKVKELF 323 >B1L1X9.1 B1L1X9_CLOBM TPP-dependent acetoin dehydrogenase complex, E1 component, beta subunit EC=1.1.1.- Length = 323 Score = 422 bits (1085), Expect = e-116, Method: Compositional matrix adjust. Identities = 197/323 (60%), Positives = 253/323 (78%) Query: 1 MRKMTYGEGIREGMRSKMLEDPNVVLLGEDVGPYGGTFGVTKGLWEEFGEERVRDTPISE 60 M+K+TY E IRE MR+KM ED V++ GEDVG +GG FGVT L+ EFG++RVRDTPISE Sbjct: 1 MKKLTYAEAIREAMRTKMSEDDKVLIFGEDVGAFGGCFGVTGDLYAEFGDKRVRDTPISE 60 Query: 61 XXXXXXXXXXXXXXLRPVAELMFIDFLTFGMDGLVNQAAKMRYMFGGKISVPLVLRLPAG 120 LRP+AE+MF DFLT MD +VNQAAKMR+MFGGKIS+P+V+RLP G Sbjct: 61 GAIAGCAVGAAATGLRPIAEIMFGDFLTVSMDMIVNQAAKMRFMFGGKISLPMVVRLPEG 120 Query: 121 GGISAGAQHSGSLEAWVTHVPGLKVVYPSNAQDAWGMMLTAIEDDNPVVYIESKVLYSKK 180 G+ A AQHS SLEAW+THVPGLKVVYPS QDA+G+M+ AIEDDNPV+++E K LY K Sbjct: 121 AGVQAAAQHSQSLEAWLTHVPGLKVVYPSTPQDAYGLMVAAIEDDNPVMFMEHKFLYGMK 180 Query: 181 FEVDDTVAAVPFGVASVKREGSDVSIITYGKQVDDALNAAKVLAEEGIEAEVIDLRSLYP 240 EV D + +P GVA +KREG DV+II GK V ++L AA+ L++EGIEAE++D R+LYP Sbjct: 181 GEVSDEIKRIPLGVADIKREGKDVTIIATGKMVHESLKAAETLSKEGIEAEIVDPRTLYP 240 Query: 241 LDKEAIFSSVAKTHRALVVTEEVKRGGYGGELSAMISELCFDELDAPVVRIGALDTPVPY 300 LDKE IF+S+ KT+RA++VTEE KRG Y GE+S++I+E FD LDAPV RIGAL+TP+P+ Sbjct: 241 LDKETIFNSIKKTNRAVIVTEENKRGAYSGEISSLINEEIFDYLDAPVGRIGALNTPIPF 300 Query: 301 SPALEAYVLPNADDIIQGIKSMF 323 SP LE+YV+P++ DI++ +K +F Sbjct: 301 SPTLESYVIPDSKDIVKKVKELF 323 >C3KW36.1 C3KW36_CLOB6 TPP-dependent acetoin dehydrogenase complex, E1 component, beta subunit EC=1.1.1.- Length = 323 Score = 422 bits (1085), Expect = e-116, Method: Compositional matrix adjust. Identities = 200/323 (61%), Positives = 252/323 (78%) Query: 1 MRKMTYGEGIREGMRSKMLEDPNVVLLGEDVGPYGGTFGVTKGLWEEFGEERVRDTPISE 60 M+K+TY E IRE MR+KM ED V++ GEDVG + G FGVT L++EFGE+RVRDTPISE Sbjct: 1 MKKLTYAEAIREAMRTKMSEDDKVLIFGEDVGAFEGCFGVTGDLYKEFGEKRVRDTPISE 60 Query: 61 XXXXXXXXXXXXXXLRPVAELMFIDFLTFGMDGLVNQAAKMRYMFGGKISVPLVLRLPAG 120 LRP+AE+MF DFLT MD +VNQAAKMR+MFGGKIS+P+V+RLP G Sbjct: 61 GTIAGCAVGAAATGLRPIAEIMFGDFLTVSMDMIVNQAAKMRFMFGGKISLPMVVRLPEG 120 Query: 121 GGISAGAQHSGSLEAWVTHVPGLKVVYPSNAQDAWGMMLTAIEDDNPVVYIESKVLYSKK 180 G+ A AQHS SLEAW+THVPGLKVVYPS QDA+G+M++AIEDDNPV+++E K LY K Sbjct: 121 AGVQAAAQHSQSLEAWLTHVPGLKVVYPSTPQDAYGLMISAIEDDNPVMFMEHKFLYGMK 180 Query: 181 FEVDDTVAAVPFGVASVKREGSDVSIITYGKQVDDALNAAKVLAEEGIEAEVIDLRSLYP 240 EV D + +P GVA +KREG DV+II GK V ++L AA L++EGIEAEVID R+LYP Sbjct: 181 GEVSDEIKRIPLGVADIKREGKDVTIIATGKMVHESLKAADKLSKEGIEAEVIDPRTLYP 240 Query: 241 LDKEAIFSSVAKTHRALVVTEEVKRGGYGGELSAMISELCFDELDAPVVRIGALDTPVPY 300 LDKE IF S+ KT+RA+VVTEE KRG Y GE+S++I+E FD LDAPV RIGAL+TP+P+ Sbjct: 241 LDKETIFDSIKKTNRAVVVTEENKRGAYSGEISSLINEEIFDYLDAPVGRIGALNTPIPF 300 Query: 301 SPALEAYVLPNADDIIQGIKSMF 323 SP LE+YV+P++ DI++ +K +F Sbjct: 301 SPTLESYVIPDSKDIVKKVKELF 323 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.137 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,896,840,910 Number of extensions: 77529644 Number of successful extensions: 205872 Number of sequences better than 1.0e-05: 3516 Number of HSP's gapped: 202120 Number of HSP's successfully gapped: 3527 Length of query: 323 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 187 Effective length of database: 1,779,438,163 Effective search space: 332754936481 Effective search space used: 332754936481 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)