BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0554.0007.p (338 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C3KW35.1 C3KW35_CLOB6 TPP-dependent acetoin dehydrogenase comple... 486 e-135 B1QQB6.1 B1QQB6_CLOBO TPP-dependent acetoin dehydrogenase comple... 486 e-135 B1L1X8.1 B1L1X8_CLOBM TPP-dependent acetoin dehydrogenase comple... 484 e-135 A7GDV7.1 A7GDV7_CLOBL TPP-dependent acetoin dehydrogenase comple... 484 e-135 A7FUD6.1 A7FUD6_CLOB1 TPP-dependent acetoin dehydrogenase comple... 484 e-135 >C3KW35.1 C3KW35_CLOB6 TPP-dependent acetoin dehydrogenase complex, E1 component, alpha subunit EC=1.1.1.- Length = 327 Score = 486 bits (1252), Expect = e-135, Method: Compositional matrix adjust. Identities = 223/325 (68%), Positives = 265/325 (81%) Query: 12 RISKELLSGMYRKMLSIRKFEKTASTFFAEGKIPGFVHLYIGEEAIAVGACANLRDDDYI 71 ++++ + MY+ ML IRKFE+ A FAEGKIPGFVHLYIGEEA+A G CANL+D DYI Sbjct: 3 KLNENSIVEMYKTMLKIRKFEQVAMNTFAEGKIPGFVHLYIGEEAVATGVCANLKDSDYI 62 Query: 72 TSTHRGHGHIVAKGGNLKYMMAELFGKETGYCKGKGGSMHIADAELGILGANGIVGAGHL 131 TSTHRGHGHI+AKGG+LK+MMAELFGK TGYCKGKGGSMHIADA GILGANGIVGAGH Sbjct: 63 TSTHRGHGHILAKGGDLKFMMAELFGKVTGYCKGKGGSMHIADATKGILGANGIVGAGHN 122 Query: 132 IATGAAWSASYRGSDQVSVCFFGDASTNQSTFHEAMNLASLWKLPVIFVCENNLYGISVS 191 IA GA SA YRG+DQV VCFFGDASTNQ TFHE++N+AS+WKLPV+FVCENNLYGIS+S Sbjct: 123 IAVGAGLSAQYRGTDQVCVCFFGDASTNQGTFHESLNMASVWKLPVVFVCENNLYGISMS 182 Query: 192 QARHQTIKDIAKRGEGYCMPSFTADGNDVITVYEHVQEAVRRARNGEGPTLLEFKTYRQH 251 Q RHQ IKD+A RG Y +P DGNDV VYE +EA++RAR G+GPTL+E KTYR Sbjct: 183 QNRHQAIKDVADRGVAYNVPGIVVDGNDVFAVYEAAKEAIKRAREGKGPTLIECKTYRHR 242 Query: 252 GHFEGDPGAYRPKEEVEAWLQKDPIPRFEKYLTENKLMTADELKQIGDEVDGEIQEALDF 311 GHFEGDP Y+P E E WL KDPIPRFEKYL EN+++T +ELK++ ++V+GEI EA+DF Sbjct: 243 GHFEGDPCVYKPTAEQEEWLAKDPIPRFEKYLVENEILTEEELKEVQNKVEGEIDEAVDF 302 Query: 312 AFASGYPALEASVQGIYSDIVEEAR 336 A S YP LE+ ++ +Y+DI EE R Sbjct: 303 ANNSPYPELESVLEDVYTDIKEEVR 327 >B1QQB6.1 B1QQB6_CLOBO TPP-dependent acetoin dehydrogenase complex, E1 component, alpha subunit EC=1.1.1.- Length = 327 Score = 486 bits (1252), Expect = e-135, Method: Compositional matrix adjust. Identities = 223/325 (68%), Positives = 265/325 (81%) Query: 12 RISKELLSGMYRKMLSIRKFEKTASTFFAEGKIPGFVHLYIGEEAIAVGACANLRDDDYI 71 ++++ + MY+ ML IRKFE+ A FAEGKIPGFVHLYIGEEA+A G CANL+D DYI Sbjct: 3 KLNENSIVEMYKTMLKIRKFEQVAMNTFAEGKIPGFVHLYIGEEAVATGVCANLKDSDYI 62 Query: 72 TSTHRGHGHIVAKGGNLKYMMAELFGKETGYCKGKGGSMHIADAELGILGANGIVGAGHL 131 TSTHRGHGHI+AKGG+LK+MMAELFGK TGYCKGKGGSMHIADA GILGANGIVGAGH Sbjct: 63 TSTHRGHGHILAKGGDLKFMMAELFGKVTGYCKGKGGSMHIADATKGILGANGIVGAGHN 122 Query: 132 IATGAAWSASYRGSDQVSVCFFGDASTNQSTFHEAMNLASLWKLPVIFVCENNLYGISVS 191 IA GA SA YRG+DQV VCFFGDASTNQ TFHE++N+AS+WKLPV+FVCENNLYGIS+S Sbjct: 123 IAVGAGLSAQYRGTDQVCVCFFGDASTNQGTFHESLNMASVWKLPVVFVCENNLYGISMS 182 Query: 192 QARHQTIKDIAKRGEGYCMPSFTADGNDVITVYEHVQEAVRRARNGEGPTLLEFKTYRQH 251 Q RHQ IKD+A RG Y +P DGNDV VYE +EA++RAR G+GPTL+E KTYR Sbjct: 183 QNRHQAIKDVADRGVAYNVPGIVVDGNDVFAVYEAAKEAIKRAREGKGPTLIECKTYRHR 242 Query: 252 GHFEGDPGAYRPKEEVEAWLQKDPIPRFEKYLTENKLMTADELKQIGDEVDGEIQEALDF 311 GHFEGDP Y+P E E WL KDPIPRFEKYL EN+++T +ELK++ ++V+GEI EA+DF Sbjct: 243 GHFEGDPCVYKPTAEQEEWLAKDPIPRFEKYLVENEILTEEELKEVQNKVEGEIDEAVDF 302 Query: 312 AFASGYPALEASVQGIYSDIVEEAR 336 A S YP LE+ ++ +Y+DI EE R Sbjct: 303 ANNSPYPELESVLEDVYTDIKEEVR 327 >B1L1X8.1 B1L1X8_CLOBM TPP-dependent acetoin dehydrogenase complex, E1 component, alpha subunit EC=1.1.1.- Length = 327 Score = 484 bits (1247), Expect = e-135, Method: Compositional matrix adjust. Identities = 221/325 (68%), Positives = 265/325 (81%) Query: 12 RISKELLSGMYRKMLSIRKFEKTASTFFAEGKIPGFVHLYIGEEAIAVGACANLRDDDYI 71 ++++ + MY+ ML IRKFE+ A FAEGKIPGFVHLYIGEEA+A G CANL+D DYI Sbjct: 3 KLNENSIVEMYKTMLKIRKFEQVAMNTFAEGKIPGFVHLYIGEEAVATGVCANLKDSDYI 62 Query: 72 TSTHRGHGHIVAKGGNLKYMMAELFGKETGYCKGKGGSMHIADAELGILGANGIVGAGHL 131 TSTHRGHGHI+AKGG+LK+MMAELFGK TGYCKGKGGSMHIADA GILGANGIVGAGH Sbjct: 63 TSTHRGHGHILAKGGDLKFMMAELFGKATGYCKGKGGSMHIADATKGILGANGIVGAGHN 122 Query: 132 IATGAAWSASYRGSDQVSVCFFGDASTNQSTFHEAMNLASLWKLPVIFVCENNLYGISVS 191 IA GA SA YRG+DQV VCFFGDASTNQ TFHE++N+AS+WKLPV+FVCENNLYGIS+S Sbjct: 123 IAVGAGLSAQYRGTDQVCVCFFGDASTNQGTFHESLNMASVWKLPVVFVCENNLYGISMS 182 Query: 192 QARHQTIKDIAKRGEGYCMPSFTADGNDVITVYEHVQEAVRRARNGEGPTLLEFKTYRQH 251 Q RHQ IKD+A RG Y +P DGNDV VYE +EA++RAR G+GPTL+E KTYR Sbjct: 183 QNRHQAIKDVADRGVAYNVPGIVVDGNDVFAVYEAAKEAIKRAREGKGPTLIECKTYRHR 242 Query: 252 GHFEGDPGAYRPKEEVEAWLQKDPIPRFEKYLTENKLMTADELKQIGDEVDGEIQEALDF 311 GHFEGDP Y+P EE E WL KDPIPRFEKYL EN+++T ++LK++ ++V+ +I EA+DF Sbjct: 243 GHFEGDPCVYKPTEEQEEWLAKDPIPRFEKYLVENEILTEEKLKEVQNKVESQIDEAVDF 302 Query: 312 AFASGYPALEASVQGIYSDIVEEAR 336 A S YP LE+ ++ +Y+DI EE R Sbjct: 303 ANNSPYPELESVLEDVYTDIKEEVR 327 >A7GDV7.1 A7GDV7_CLOBL TPP-dependent acetoin dehydrogenase complex, E1 component, alpha subunit EC=1.1.1.- Length = 327 Score = 484 bits (1247), Expect = e-135, Method: Compositional matrix adjust. Identities = 221/325 (68%), Positives = 265/325 (81%) Query: 12 RISKELLSGMYRKMLSIRKFEKTASTFFAEGKIPGFVHLYIGEEAIAVGACANLRDDDYI 71 ++++ + MY+ ML IRKFE+ A FAEGKIPGFVHLYIGEEA+A G CANL+D DYI Sbjct: 3 KLNENSIVEMYKTMLKIRKFEQVAMNTFAEGKIPGFVHLYIGEEAVATGVCANLKDSDYI 62 Query: 72 TSTHRGHGHIVAKGGNLKYMMAELFGKETGYCKGKGGSMHIADAELGILGANGIVGAGHL 131 TSTHRGHGHI+AKGG+LK+MMAELFGK TGYCKGKGGSMHIADA GILGANGIVGAGH Sbjct: 63 TSTHRGHGHILAKGGDLKFMMAELFGKATGYCKGKGGSMHIADATKGILGANGIVGAGHN 122 Query: 132 IATGAAWSASYRGSDQVSVCFFGDASTNQSTFHEAMNLASLWKLPVIFVCENNLYGISVS 191 IA GA SA YRG+DQV VCFFGDASTNQ TFHE++N+AS+WKLPV+FVCENNLYGIS+S Sbjct: 123 IAVGAGLSAQYRGTDQVCVCFFGDASTNQGTFHESLNMASVWKLPVVFVCENNLYGISMS 182 Query: 192 QARHQTIKDIAKRGEGYCMPSFTADGNDVITVYEHVQEAVRRARNGEGPTLLEFKTYRQH 251 Q RHQ IKD+A RG Y +P DGNDV VYE +EA++RAR G+GPTL+E KTYR Sbjct: 183 QNRHQAIKDVADRGVAYNVPGIVVDGNDVFAVYEAAKEAIKRAREGKGPTLIECKTYRHR 242 Query: 252 GHFEGDPGAYRPKEEVEAWLQKDPIPRFEKYLTENKLMTADELKQIGDEVDGEIQEALDF 311 GHFEGDP Y+P EE E WL KDPIPRFEKYL EN+++T ++LK++ ++V+ +I EA+DF Sbjct: 243 GHFEGDPCVYKPTEEQEEWLAKDPIPRFEKYLVENEILTEEKLKEVQNKVESQIDEAVDF 302 Query: 312 AFASGYPALEASVQGIYSDIVEEAR 336 A S YP LE+ ++ +Y+DI EE R Sbjct: 303 ANNSPYPELESVLEDVYTDIKEEVR 327 >A7FUD6.1 A7FUD6_CLOB1 TPP-dependent acetoin dehydrogenase complex, E1 component, alpha subunit EC=1.1.1.- Length = 327 Score = 484 bits (1247), Expect = e-135, Method: Compositional matrix adjust. Identities = 221/325 (68%), Positives = 265/325 (81%) Query: 12 RISKELLSGMYRKMLSIRKFEKTASTFFAEGKIPGFVHLYIGEEAIAVGACANLRDDDYI 71 ++++ + MY+ ML IRKFE+ A FAEGKIPGFVHLYIGEEA+A G CANL+D DYI Sbjct: 3 KLNENSIVEMYKTMLKIRKFEQVAMNTFAEGKIPGFVHLYIGEEAVATGVCANLKDSDYI 62 Query: 72 TSTHRGHGHIVAKGGNLKYMMAELFGKETGYCKGKGGSMHIADAELGILGANGIVGAGHL 131 TSTHRGHGHI+AKGG+LK+MMAELFGK TGYCKGKGGSMHIADA GILGANGIVGAGH Sbjct: 63 TSTHRGHGHILAKGGDLKFMMAELFGKATGYCKGKGGSMHIADATKGILGANGIVGAGHN 122 Query: 132 IATGAAWSASYRGSDQVSVCFFGDASTNQSTFHEAMNLASLWKLPVIFVCENNLYGISVS 191 IA GA SA YRG+DQV VCFFGDASTNQ TFHE++N+AS+WKLPV+FVCENNLYGIS+S Sbjct: 123 IAVGAGLSAQYRGTDQVCVCFFGDASTNQGTFHESLNMASVWKLPVVFVCENNLYGISMS 182 Query: 192 QARHQTIKDIAKRGEGYCMPSFTADGNDVITVYEHVQEAVRRARNGEGPTLLEFKTYRQH 251 Q RHQ IKD+A RG Y +P DGNDV VYE +EA++RAR G+GPTL+E KTYR Sbjct: 183 QNRHQAIKDVADRGVAYNVPGIVVDGNDVFAVYEAAKEAIKRAREGKGPTLIECKTYRHR 242 Query: 252 GHFEGDPGAYRPKEEVEAWLQKDPIPRFEKYLTENKLMTADELKQIGDEVDGEIQEALDF 311 GHFEGDP Y+P EE E WL KDPIPRFEKYL EN+++T ++LK++ ++V+ +I EA+DF Sbjct: 243 GHFEGDPCVYKPTEEQEEWLAKDPIPRFEKYLVENEILTEEKLKEVQNKVESQIDEAVDF 302 Query: 312 AFASGYPALEASVQGIYSDIVEEAR 336 A S YP LE+ ++ +Y+DI EE R Sbjct: 303 ANNSPYPELESVLEDVYTDIKEEVR 327 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,115,471,123 Number of extensions: 93626074 Number of successful extensions: 238467 Number of sequences better than 1.0e-05: 2171 Number of HSP's gapped: 235029 Number of HSP's successfully gapped: 2182 Length of query: 338 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 202 Effective length of database: 1,779,438,163 Effective search space: 359446508926 Effective search space used: 359446508926 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)