BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0554.0005.p (159 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K5S0.1 B3K5S0_9BACI PTS system, trehalose-specific IIBC subunit 157 4e-37 B3KIS7.1 B3KIS7_9BACI PTS system, glucose subfamily, IIA subunit 132 1e-29 Q7A807.1 PTG3C_STAAN PTS system glucose-specific EIICBA componen... 123 5e-27 Q99X32.1 PTG3C_STAAM PTS system glucose-specific EIICBA componen... 123 5e-27 A5IP58.1 PTG3C_STAA9 PTS system glucose-specific EIICBA componen... 123 5e-27 >B3K5S0.1 B3K5S0_9BACI PTS system, trehalose-specific IIBC subunit Length = 678 Score = 157 bits (396), Expect = 4e-37, Method: Compositional matrix adjust. Identities = 77/153 (50%), Positives = 111/153 (72%), Gaps = 5/153 (3%) Query: 10 EENTLDIISPVPGQVVGLEQVPDEAFSTKAMGEGFAVQPIKGEVRAPFSGKISHIMNRSN 69 ++ +++ +P+ G+VV L +VPD AF+ K MG+G A+ P +G+V APF G+++H++ +S Sbjct: 520 QQGAVEVKAPIKGKVVPLSEVPDPAFADKQMGQGIAIIPSEGKVYAPFDGQVAHLIKKSK 579 Query: 70 HAILLENEAGIQILIHVGVDTVSLKGEGFVPHVVTGQTVEQGELLLEFDIARIQEAGLSD 129 HAI+L + GIQILIHVG++TVSLKGEGF HV TG + +G+LLLEFDI RIQ+AGL Sbjct: 580 HAIILTDANGIQILIHVGINTVSLKGEGFTAHVETGAEIRKGQLLLEFDIDRIQQAGLPV 639 Query: 130 ITSVIVPGGQEKVQRI-----EGLQDEDAVLRI 157 IT VI+P GQ+ +Q + E L E VL++ Sbjct: 640 ITPVIIPDGQDHIQNVTEFEGEALPGETRVLQV 672 >B3KIS7.1 B3KIS7_9BACI PTS system, glucose subfamily, IIA subunit Length = 165 Score = 132 bits (331), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 64/148 (43%), Positives = 97/148 (65%), Gaps = 2/148 (1%) Query: 1 MFGWRKNKIEENTLDIISPVPGQVVGLEQVPDEAFSTKAMGEGFAVQPIKGEVRAPFSGK 60 MF K K + + +P+ G+ V L QVPDEAF+ MG+G A++P +G++ APF G Sbjct: 1 MFDRWKKKKTATVIQLTAPLSGKAVPLSQVPDEAFAAGHMGQGIAIEPSEGKLIAPFDGT 60 Query: 61 ISHIMNRSNHAILLENEA-GIQILIHVGVDTVSLKGEGFVPHVVTGQTVEQGELLLEFDI 119 ++H++ +S HA++LE+ A G+Q L H+G++TV LKGEGF HV G V+ G+ L+EFD+ Sbjct: 61 VAHVI-KSKHAVMLEDPATGLQYLFHIGINTVGLKGEGFTSHVQAGDQVKSGQTLIEFDM 119 Query: 120 ARIQEAGLSDITSVIVPGGQEKVQRIEG 147 A I+ AG IT +IV E +++G Sbjct: 120 AAIRAAGYPTITPIIVTNADEVASKVDG 147 >Q7A807.1 PTG3C_STAAN PTS system glucose-specific EIICBA component Glucose permease IIC component Glucose-specific phosphotransferase enzyme IIB component Glucose-specific phosphotransferase enzyme IIA component EC=2.7.1.69 EC=2.7.1.- Length = 681 Score = 123 bits (309), Expect = 5e-27, Method: Compositional matrix adjust. Identities = 64/134 (47%), Positives = 92/134 (68%), Gaps = 1/134 (0%) Query: 6 KNKIEENTLDIISPVPGQVVGLEQVPDEAFSTKAMGEGFAVQPIKGEVRAPFSGKISHIM 65 K+ E + + +P+ G+V L +VPD+ FS K MG+G A++P +GEVRAPF+GKI I Sbjct: 525 KSATSELSHIVHAPLTGEVTPLSEVPDQVFSEKMMGDGIAIKPSQGEVRAPFNGKIQMIF 584 Query: 66 NRSNHAILLENEAGIQILIHVGVDTVSLKGEGFVPHVVTGQTVEQGELLLEFDIARIQEA 125 + HAI L +++G+++LIH+G+DTV L GEGF HV GQ V+QG+LL+ FD+ I+ Sbjct: 585 -PTKHAIGLVSDSGLELLIHIGLDTVKLNGEGFTLHVEEGQEVKQGDLLINFDLDYIRNH 643 Query: 126 GLSDITSVIVPGGQ 139 SDIT +IV G Sbjct: 644 AKSDITPIIVTQGN 657 >Q99X32.1 PTG3C_STAAM PTS system glucose-specific EIICBA component Glucose permease IIC component Glucose-specific phosphotransferase enzyme IIB component Glucose-specific phosphotransferase enzyme IIA component EC=2.7.1.69 EC=2.7.1.- Length = 681 Score = 123 bits (309), Expect = 5e-27, Method: Compositional matrix adjust. Identities = 64/134 (47%), Positives = 92/134 (68%), Gaps = 1/134 (0%) Query: 6 KNKIEENTLDIISPVPGQVVGLEQVPDEAFSTKAMGEGFAVQPIKGEVRAPFSGKISHIM 65 K+ E + + +P+ G+V L +VPD+ FS K MG+G A++P +GEVRAPF+GKI I Sbjct: 525 KSATSELSHIVHAPLTGEVTPLSEVPDQVFSEKMMGDGIAIKPSQGEVRAPFNGKIQMIF 584 Query: 66 NRSNHAILLENEAGIQILIHVGVDTVSLKGEGFVPHVVTGQTVEQGELLLEFDIARIQEA 125 + HAI L +++G+++LIH+G+DTV L GEGF HV GQ V+QG+LL+ FD+ I+ Sbjct: 585 -PTKHAIGLVSDSGLELLIHIGLDTVKLNGEGFTLHVEEGQEVKQGDLLINFDLDYIRNH 643 Query: 126 GLSDITSVIVPGGQ 139 SDIT +IV G Sbjct: 644 AKSDITPIIVTQGN 657 >A5IP58.1 PTG3C_STAA9 PTS system glucose-specific EIICBA component Glucose permease IIC component Glucose-specific phosphotransferase enzyme IIB component Glucose-specific phosphotransferase enzyme IIA component EC=2.7.1.69 EC=2.7.1.- Length = 681 Score = 123 bits (309), Expect = 5e-27, Method: Compositional matrix adjust. Identities = 64/134 (47%), Positives = 92/134 (68%), Gaps = 1/134 (0%) Query: 6 KNKIEENTLDIISPVPGQVVGLEQVPDEAFSTKAMGEGFAVQPIKGEVRAPFSGKISHIM 65 K+ E + + +P+ G+V L +VPD+ FS K MG+G A++P +GEVRAPF+GKI I Sbjct: 525 KSATSELSHIVHAPLTGEVTPLSEVPDQVFSEKMMGDGIAIKPSQGEVRAPFNGKIQMIF 584 Query: 66 NRSNHAILLENEAGIQILIHVGVDTVSLKGEGFVPHVVTGQTVEQGELLLEFDIARIQEA 125 + HAI L +++G+++LIH+G+DTV L GEGF HV GQ V+QG+LL+ FD+ I+ Sbjct: 585 -PTKHAIGLVSDSGLELLIHIGLDTVKLNGEGFTLHVEEGQEVKQGDLLINFDLDYIRNH 643 Query: 126 GLSDITSVIVPGGQ 139 SDIT +IV G Sbjct: 644 AKSDITPIIVTQGN 657 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.138 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 933,282,543 Number of extensions: 37049859 Number of successful extensions: 92311 Number of sequences better than 1.0e-05: 1944 Number of HSP's gapped: 90420 Number of HSP's successfully gapped: 1944 Length of query: 159 Length of database: 3,060,696,203 Length adjustment: 120 Effective length of query: 39 Effective length of database: 1,930,174,403 Effective search space: 75276801717 Effective search space used: 75276801717 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 125 (52.8 bits)