BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0554.0002.p (489 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q3Y1U6.1 Q3Y1U6_ENTFC Sugar-specific permease, EIIA 1 domain 484 e-135 C2HD54.1 C2HD54_ENTFC Protein-N(Pi)-phosphohistidine--sugar phos... 480 e-133 C2C2H0.1 C2C2H0_LISGR Protein-N(Pi)-phosphohistidine--sugar phos... 478 e-133 Q4L8H5.1 PTXBC_STAHJ PTS system EIIBC component SH0741 Phosphotr... 478 e-133 Q4EFS4.1 Q4EFS4_LISMO PTS system, sucrose-specific, IIBC component 474 e-132 >Q3Y1U6.1 Q3Y1U6_ENTFC Sugar-specific permease, EIIA 1 domain Length = 660 Score = 484 bits (1246), Expect = e-135, Method: Compositional matrix adjust. Identities = 243/487 (49%), Positives = 346/487 (71%), Gaps = 11/487 (2%) Query: 5 QDLAHGILSTVGGIQNVELITHCMTRVRITLRDESYASIEDLKRVPGVIGVIEESTLQII 64 ++LA I GG+ NVE I HCMTRVR+ + ++ ++ +LK +PGV+GV+E+ LQ+I Sbjct: 9 EELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDLVNVSELKAIPGVLGVVEDEQLQVI 68 Query: 65 VGPGLVEKVSRVLADISGVELGEP---VPLQAAHAFPNPDSRINGLSDKEKAEQLAAXXX 121 +GPG V KV++ + D++GV+LGE V A++ + R+N + + KA Q + Sbjct: 69 IGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATANSSASGKDRVNERAQEMKAAQKSKQKP 128 Query: 122 XXXXXXXXTPVKNFLKSISNIFIPLAPAFVAAGLLAGITSVIGNFITAGHLSGEGWAQFN 181 + +K+ LK ISNIF+P+ PAFV G++AGI +V+ N +TAG L W Q+ Sbjct: 129 --------SKIKSVLKDISNIFVPMIPAFVGTGIVAGIAAVLANLVTAGTLDAATWQQYI 180 Query: 182 LILNVIKNGIFSYMAIFVGINSARVFGASPSLGGVIGGATLLTGMKPELPIHNLFTGEPL 241 ++N++KNG+FSY+ I+ GIN+A+VFGA+P+LGGVIG +LTGM PE P+ NLFTGE L Sbjct: 181 DVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGAVVMLTGMNPEAPLKNLFTGEAL 240 Query: 242 IAGQGGIIGVVLAVWILALIEKRLHKIMPNSIDLIVTSFLSVLATGLLTIFLIMPLAGFV 301 AGQGGI+GV+ AVW+L+++EK+LHK++P+++D+IVT LS++A GL+ IF IMPLAGF+ Sbjct: 241 AAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIVTPTLSLIAIGLIEIFFIMPLAGFI 300 Query: 302 ADNLLVAVNWILSVXXXXXXXXXXXIWLVMVMLGLHHIMTPIHIELIAQTGMTILLPIXX 361 +D ++ +NW+L V ++L MVM GLH I+TPIH+++I +TG T+LLPI Sbjct: 301 SDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFGLHQILTPIHVQMIDETGRTLLLPILA 360 Query: 362 XXXXXXXXXXXXXWIRCRKNKQLTGIIKGALPVGVLGIGEPLIYGVTLPLGKPFVTACLG 421 WIRC+K+K+LT +IKGALPVG+LGIGEPLIYGVTLPLG+PF+TAC+G Sbjct: 361 MAGAGQVGAALALWIRCKKDKELTEMIKGALPVGILGIGEPLIYGVTLPLGRPFITACIG 420 Query: 422 GAFGGAVIGYFGNVGAMSIGASGIEMLLLIADGKWWVYLLGLITAYFFGFICTYLFGTPK 481 G GGAVIG GNVGA++IG SG ++ LI+DGKW Y+LGL+ AY GF+ T+ FG PK Sbjct: 421 GGIGGAVIGMIGNVGAIAIGPSGAALIPLISDGKWLGYILGLLAAYAGGFVATFFFGIPK 480 Query: 482 EARQAKE 488 + + +E Sbjct: 481 DRLEKEE 487 >C2HD54.1 C2HD54_ENTFC Protein-N(Pi)-phosphohistidine--sugar phosphotransferase Length = 660 Score = 480 bits (1235), Expect = e-133, Method: Compositional matrix adjust. Identities = 243/487 (49%), Positives = 346/487 (71%), Gaps = 11/487 (2%) Query: 5 QDLAHGILSTVGGIQNVELITHCMTRVRITLRDESYASIEDLKRVPGVIGVIEESTLQII 64 ++LA I GG+ NVE I HCMTRVR+ + ++ ++ +LK +PGV+GV+E+ LQ+I Sbjct: 9 EELAKQIYQGAGGMGNVESIVHCMTRVRMKVINDDLVNVSELKAIPGVLGVVEDEQLQVI 68 Query: 65 VGPGLVEKVSRVLADISGVELGEPVPLQAAHAFPNP---DSRINGLSDKEKAEQLAAXXX 121 +GPG V KV++ + D++GV+LGE +P +A + R+N + + KA Q + Sbjct: 69 IGPGKVNKVAQEMVDLAGVKLGEKLPSASATTASSSASGKDRVNERAQEMKAAQKSKQKP 128 Query: 122 XXXXXXXXTPVKNFLKSISNIFIPLAPAFVAAGLLAGITSVIGNFITAGHLSGEGWAQFN 181 + +K+ LK ISNIF+P+ PAFV G++AGI +V+ N +TAG L W Q+ Sbjct: 129 --------SKIKSVLKDISNIFVPMIPAFVGTGIVAGIAAVLANLVTAGTLDAGTWQQYI 180 Query: 182 LILNVIKNGIFSYMAIFVGINSARVFGASPSLGGVIGGATLLTGMKPELPIHNLFTGEPL 241 ++N++KNG+FSY+ I+ GIN+A+VFGA+P+LGGVIG +LTGM PE P+ NLFTGE L Sbjct: 181 DVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGAVVMLTGMNPEAPLKNLFTGEAL 240 Query: 242 IAGQGGIIGVVLAVWILALIEKRLHKIMPNSIDLIVTSFLSVLATGLLTIFLIMPLAGFV 301 AGQGGI+GV+ AVW+L+++EK+LHK++P+++D+IVT LS++A GL+ IF IMPLAGF+ Sbjct: 241 AAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIVTPTLSLIAIGLIEIFFIMPLAGFI 300 Query: 302 ADNLLVAVNWILSVXXXXXXXXXXXIWLVMVMLGLHHIMTPIHIELIAQTGMTILLPIXX 361 +D ++ +NW+L V ++L MVM GLH I+TPIH+++I +TG T+LLPI Sbjct: 301 SDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFGLHQILTPIHVQMIDETGRTLLLPILA 360 Query: 362 XXXXXXXXXXXXXWIRCRKNKQLTGIIKGALPVGVLGIGEPLIYGVTLPLGKPFVTACLG 421 WIRC+K+K+LT +IKGALPVG+LGIGEPLIYGVTLPLG+PF+TAC+G Sbjct: 361 MAGAGQVGAALALWIRCKKDKELTEMIKGALPVGILGIGEPLIYGVTLPLGRPFITACIG 420 Query: 422 GAFGGAVIGYFGNVGAMSIGASGIEMLLLIADGKWWVYLLGLITAYFFGFICTYLFGTPK 481 G GGAVIG GNVGA++IG SG ++ LI+DGKW Y+LGL+ AY GF+ T+ FG PK Sbjct: 421 GGIGGAVIGMIGNVGAIAIGPSGAALIPLISDGKWLGYILGLLAAYAGGFVATFFFGIPK 480 Query: 482 EARQAKE 488 + + +E Sbjct: 481 DRLEKEE 487 >C2C2H0.1 C2C2H0_LISGR Protein-N(Pi)-phosphohistidine--sugar phosphotransferase EC=2.7.1.69 Length = 486 Score = 478 bits (1230), Expect = e-133, Method: Compositional matrix adjust. Identities = 252/490 (51%), Positives = 342/490 (69%), Gaps = 7/490 (1%) Query: 1 MSKEQDLAHGILSTVGGIQNVELITHCMTRVRITLRDESYASIEDLKRVPGVIGVIEEST 60 M+KEQ++A I S VGG+ N+ I HCMTRVR+ ++D + S+E+LK +PGV+GV+E+ T Sbjct: 1 MTKEQEIAKQITSKVGGLGNISRIAHCMTRVRLGIKDPAKVSLEELKAIPGVLGVVEDET 60 Query: 61 LQIIVGPGLVEKVSRVLADISGVELGEPVPLQAAHAFPNPDSRINGLSDKEKAEQLAAXX 120 LQIIVGPG+V KV++ +AD++GV++G+ +P H + + S KE E+ AA Sbjct: 61 LQIIVGPGVVNKVAKEMADMAGVKIGDTIPENLDHELTDTNK-----SGKELVEERAAAT 115 Query: 121 XXXXXXXX--XTPVKNFLKSISNIFIPLAPAFVAAGLLAGITSVIGNFITAGHLSGEGWA 178 + K LKS+SNIF+PL P FV AGL+AGI ++I N I A +L W Sbjct: 116 KAEMKAKQNNSSSFKRLLKSVSNIFVPLIPGFVGAGLIAGIAAIITNNILAKNLDAATWN 175 Query: 179 QFNLILNVIKNGIFSYMAIFVGINSARVFGASPSLGGVIGGATLLTGMKPELPIHNLFTG 238 QF IL V+ GIF+++AI+VGIN+A FG +P LGG + G TLL G+ I N+FTG Sbjct: 176 QFVDILGVLNKGIFAFLAIYVGINTANEFGGTPILGGGVAGVTLLPGLLDGHIIKNIFTG 235 Query: 239 EPLIAGQGGIIGVVLAVWILALIEKRLHKIMPNSIDLIVTSFLSVLATGLLTIFLIMPLA 298 P+ AGQGGIIGV+ AVW++ ++E+ L K++PN+ID+IVT + +L GL+TIFLIMP+A Sbjct: 236 APITAGQGGIIGVLFAVWLMCVLERNLRKVIPNAIDIIVTPTIILLVIGLITIFLIMPVA 295 Query: 299 GFVADNLLVAVNWILSVXXXXXXXXXXXIWLVMVMLGLHHIMTPIHIELIAQTGMTILLP 358 G V+ L+ A+NW++SV ++L MVMLGLH ++TPIH+E+I QTG T+LLP Sbjct: 296 GLVSTWLVTAINWVISVGGVFAGFVLGTLFLPMVMLGLHQVLTPIHVEMINQTGYTVLLP 355 Query: 359 IXXXXXXXXXXXXXXXWIRCRKNKQLTGIIKGALPVGVLGIGEPLIYGVTLPLGKPFVTA 418 I WIRCRKNK L +IKG LPVG+LGIGEPLIYGVT+PLG+PF+TA Sbjct: 356 ILAMAGGGQVGAAIALWIRCRKNKPLVTMIKGGLPVGILGIGEPLIYGVTIPLGRPFITA 415 Query: 419 CLGGAFGGAVIGYFGNVGAMSIGASGIEMLLLIADGKWWVYLLGLITAYFFGFICTYLFG 478 C+GG GGAV+G+FGNVGA++IG SG+ ++ LIA+ W Y++GLI AY GFI TYLFG Sbjct: 416 CIGGGIGGAVVGFFGNVGAIAIGPSGVALIPLIANNMWLAYVIGLIAAYIGGFILTYLFG 475 Query: 479 TPKEARQAKE 488 TPK+A +A E Sbjct: 476 TPKDAMEAVE 485 >Q4L8H5.1 PTXBC_STAHJ PTS system EIIBC component SH0741 Phosphotransferase enzyme IIB component Permease IIC component EC=2.7.1.69 Length = 477 Score = 478 bits (1230), Expect = e-133, Method: Compositional matrix adjust. Identities = 260/488 (53%), Positives = 338/488 (69%), Gaps = 14/488 (2%) Query: 1 MSKEQDLAHGILSTVGGIQNVELITHCMTRVRITLRDESYASIEDLKRVPGVIGVIEEST 60 M+K+Q LAH IL VGGI NV+ I HCMTRVR+ + +E+ LK + GV+GVI++ Sbjct: 1 MNKDQQLAHHILDAVGGIDNVDNIIHCMTRVRLKINNETQVDYPKLKNIEGVLGVIQDER 60 Query: 61 LQIIVGPGLVEKVSRVLADISGVELGEPVPLQAAHAFPNPDSRINGLSDKEKAEQLAAXX 120 LQI+VGPG V +VS + +SGV+LGE +P ++ + ++ N ++K +Q Sbjct: 61 LQIVVGPGTVNEVSAEMVKLSGVQLGEDIPHRSNTSNIKNQAQQNKREFQQKRKQ----- 115 Query: 121 XXXXXXXXXTPVKNFLKSISNIFIPLAPAFVAAGLLAGITSVIGNFITAGHLSGEGWAQF 180 + + LKSI+NIFIPL PAF+ AGL+ GI +V+ NFITAG +S + Q Sbjct: 116 ---------SKMNTILKSIANIFIPLIPAFIGAGLIGGIAAVLNNFITAGTISADWVKQL 166 Query: 181 NLILNVIKNGIFSYMAIFVGINSARVFGASPSLGGVIGGATLLTGMKPELPIHNLFTGEP 240 +LNVIK+G+ +Y+AIF G N+A+VFGA+P LGGVIGG TLLTG+ + PI N+FTGEP Sbjct: 167 VAVLNVIKDGMLAYLAIFTGFNAAKVFGATPGLGGVIGGTTLLTGITEDNPIKNVFTGEP 226 Query: 241 LIAGQGGIIGVVLAVWILALIEKRLHKIMPNSIDLIVTSFLSVLATGLLTIFLIMPLAGF 300 LIAGQGGIIGV+LAVW+L++IEK+LHKI+PN+ID+IVT +S+L GLLTIF MP+AGF Sbjct: 227 LIAGQGGIIGVILAVWLLSIIEKKLHKIVPNAIDIIVTPTISLLIIGLLTIFFFMPIAGF 286 Query: 301 VADNLLVAVNWILSVXXXXXXXXXXXIWLVMVMLGLHHIMTPIHIELIAQTGMTILLPIX 360 ++D L+ VNW++ V +L +VMLGLHHI TPIHIELI Q+G T LLPI Sbjct: 287 ISDGLVGVVNWVIGVGGIFSGFIIGAFFLPLVMLGLHHIFTPIHIELINQSGATYLLPIA 346 Query: 361 XXXXXXXXXXXXXXWIRCRKNKQLTGIIKGALPVGVLGIGEPLIYGVTLPLGKPFVTACL 420 W+RC+ N L IKGALPVG LGIGEPLIYGVTLPLG+PF+TACL Sbjct: 347 AMAGAGQVGAALALWVRCKNNTTLRTAIKGALPVGFLGIGEPLIYGVTLPLGRPFITACL 406 Query: 421 GGAFGGAVIGYFGNVGAMSIGASGIEMLLLIADGKWWVYLLGLITAYFFGFICTYLFGTP 480 GG GGAVIG G++GA +IG SGI +L LIA K+ Y++GL++AY GFI TY FGT Sbjct: 407 GGGIGGAVIGGIGHIGATAIGPSGISLLPLIAHQKYLGYIIGLLSAYLAGFIFTYFFGTT 466 Query: 481 KEARQAKE 488 KE R + Sbjct: 467 KEMRNLNK 474 >Q4EFS4.1 Q4EFS4_LISMO PTS system, sucrose-specific, IIBC component Length = 482 Score = 474 bits (1221), Expect = e-132, Method: Compositional matrix adjust. Identities = 255/488 (52%), Positives = 333/488 (68%), Gaps = 7/488 (1%) Query: 1 MSKEQDLAHGILSTVGGIQNVELITHCMTRVRITLRDESYASIEDLKRVPGVIGVIEEST 60 M+KE ++ I VGG++NV I HCMTRVR+ + D ++ LK+VPGVIGV+E+ T Sbjct: 1 MTKEAEIGKQIFIHVGGMENVSRIAHCMTRVRLGIVDSDLVNVAGLKKVPGVIGVVEDDT 60 Query: 61 LQIIVGPGLVEKVSRVLADISGVELGEPVPLQAAHAFPNPDSRINGLSDKEKAEQLAAXX 120 LQIIVGPG+V KV+ +A+++GV++GE + + + K KAE A Sbjct: 61 LQIIVGPGVVNKVAGAMAEMAGVKIGETIQENLDSGTKTGKELVEEKASKTKAELKAKQN 120 Query: 121 XXXXXXXXXTPVKNFLKSISNIFIPLAPAFVAAGLLAGITSVIGNFITAGHLSGEGWAQF 180 + K LKSISNIF+PL P FV AGL+AGI ++I N ITAG+L W Q+ Sbjct: 121 NS-------SGFKRLLKSISNIFVPLIPGFVGAGLIAGIAAIISNNITAGNLDAAVWTQY 173 Query: 181 NLILNVIKNGIFSYMAIFVGINSARVFGASPSLGGVIGGATLLTGMKPELPIHNLFTGEP 240 IL VI GIF+++AI+VGIN+A FG +P LGG I G TLL+G+ I N+FTG+P Sbjct: 174 IDILGVINKGIFAFLAIYVGINTANEFGGTPVLGGGIAGITLLSGLAEGHTITNIFTGDP 233 Query: 241 LIAGQGGIIGVVLAVWILALIEKRLHKIMPNSIDLIVTSFLSVLATGLLTIFLIMPLAGF 300 ++AGQGGIIGV+LAVW++ ++EK L KI+PN+ID+I T L +L GL+TIFLIMP AG Sbjct: 234 IVAGQGGIIGVLLAVWLMCVLEKNLRKIIPNAIDIIFTPTLVLLIIGLVTIFLIMPFAGL 293 Query: 301 VADNLLVAVNWILSVXXXXXXXXXXXIWLVMVMLGLHHIMTPIHIELIAQTGMTILLPIX 360 V+D L+ +NW++ V ++L MVM GLH ++TPIH+E+IAQ G TILLPI Sbjct: 294 VSDGLVNGINWVIEVGGVFAGFVLGTLFLPMVMFGLHQVLTPIHVEMIAQNGYTILLPIL 353 Query: 361 XXXXXXXXXXXXXXWIRCRKNKQLTGIIKGALPVGVLGIGEPLIYGVTLPLGKPFVTACL 420 WIRCRKNK L +IKG LPVG+LGIGEPLIYGVT+PLGKPF+TACL Sbjct: 354 AMAGGGQVGAAIALWIRCRKNKPLVNMIKGGLPVGILGIGEPLIYGVTIPLGKPFLTACL 413 Query: 421 GGAFGGAVIGYFGNVGAMSIGASGIEMLLLIADGKWWVYLLGLITAYFFGFICTYLFGTP 480 GG GGAVIGYFGNVGA++IG SG+ ++ LIA+ +W Y++GLI AY GFI TY FGTP Sbjct: 414 GGGIGGAVIGYFGNVGAIAIGPSGVALIPLIANNEWLSYVIGLIAAYLGGFILTYFFGTP 473 Query: 481 KEARQAKE 488 K+A + E Sbjct: 474 KDAMNSVE 481 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.324 0.143 0.428 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,751,562,847 Number of extensions: 118966139 Number of successful extensions: 346218 Number of sequences better than 1.0e-05: 1702 Number of HSP's gapped: 340052 Number of HSP's successfully gapped: 1962 Length of query: 489 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 349 Effective length of database: 1,741,754,103 Effective search space: 607872181947 Effective search space used: 607872181947 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 133 (55.8 bits)