BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0553.0009.p (239 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K9U9.1 B3K9U9_9BACI Short-chain dehydrogenase/reductase SDR 328 2e-88 C1PS37.1 C1PS37_ALIAC Putative uncharacterized protein 287 5e-76 B7DP48.1 B7DP48_9BACL Short-chain dehydrogenase/reductase SDR 283 8e-75 B3ZFI2.1 B3ZFI2_BACCE Oxidoreductase, short-chain dehydrogenase/... 281 2e-74 A7GNV2.1 A7GNV2_BACCN Short-chain dehydrogenase/reductase SDR 281 3e-74 >B3K9U9.1 B3K9U9_9BACI Short-chain dehydrogenase/reductase SDR Length = 239 Score = 328 bits (841), Expect = 2e-88, Method: Compositional matrix adjust. Identities = 165/239 (69%), Positives = 201/239 (84%) Query: 1 MDLRGRSIVITGAGKGIGKALAMALAKEGANLGLISRTAADLEALKSALTEVYDIKASIA 60 M L+ ++ +ITGAGKGIG+ALA+ALAKEG ++GL++RTAADLE L + + E Y ++A A Sbjct: 1 MSLKNKTALITGAGKGIGRALAIALAKEGVHVGLVARTAADLEPLSALVAEQYGVRAVAA 60 Query: 61 VADIAVREDAERAVVSLQKDLGAFDALINNAGIAKFGTFLEMDPADWEHHMQINLFGTYY 120 VAD++V+ +AE A ++ +LG+ D LINNAGIA+FG LEM+P W+ + +NL GTYY Sbjct: 61 VADVSVQSEAEAAFAAVNMELGSVDILINNAGIAQFGNLLEMEPEAWKRIVDVNLMGTYY 120 Query: 121 VTRAALPAMIERSSGNIINISSTAGERGFATGSAYCASKFALMGMTEALAQEVRKHNIRV 180 +TRAALP MIE + G+I+NISSTAGERGFATGSAY ASKFA+MG+TEA+ QEVRKHNIRV Sbjct: 121 MTRAALPGMIEHNQGDIVNISSTAGERGFATGSAYNASKFAVMGLTEAVLQEVRKHNIRV 180 Query: 181 VALTPSTVNTGLAENAGLKIGDEDRMMQPEDVAELALAALKLPDRVFLKTAGIWTTNPQ 239 ALTPSTVNT LA NAGLKIGDEDRMMQPEDVAEL LAALKLP RVF+K+AGIWTTNPQ Sbjct: 181 TALTPSTVNTDLAVNAGLKIGDEDRMMQPEDVAELVLAALKLPQRVFVKSAGIWTTNPQ 239 >C1PS37.1 C1PS37_ALIAC Putative uncharacterized protein Length = 241 Score = 287 bits (734), Expect = 5e-76, Method: Compositional matrix adjust. Identities = 143/237 (60%), Positives = 175/237 (73%) Query: 3 LRGRSIVITGAGKGIGKALAMALAKEGANLGLISRTAADLEALKSALTEVYDIKASIAVA 62 L+G S ++TGAGKGIGKA+A LAKEG +LGLI+RT +DL + A+ Y I A Sbjct: 5 LKGWSAIVTGAGKGIGKAIAEHLAKEGVHLGLIARTESDLNRVADAIRSAYGISVYTQAA 64 Query: 63 DIAVREDAERAVVSLQKDLGAFDALINNAGIAKFGTFLEMDPADWEHHMQINLFGTYYVT 122 DIA R E A+ L++ LG+ D L+NNAG A FGT ++M +WE +++NL GTYY T Sbjct: 65 DIADRMSIESAIQRLKEQLGSVDVLVNNAGTASFGTVIDMPVEEWERIVRVNLLGTYYAT 124 Query: 123 RAALPAMIERSSGNIINISSTAGERGFATGSAYCASKFALMGMTEALAQEVRKHNIRVVA 182 RA LP MIER+ G+IINISSTAGE+G AT SAY ASKF L+G TE+L EVRKHNIRV+A Sbjct: 125 RAILPHMIERNRGHIINISSTAGEKGSATTSAYSASKFGLLGFTESLMYEVRKHNIRVMA 184 Query: 183 LTPSTVNTGLAENAGLKIGDEDRMMQPEDVAELALAALKLPDRVFLKTAGIWTTNPQ 239 L PSTVNT LA N GLK+GDED MQPEDVAEL + AL+LP+RV LK+A + TNPQ Sbjct: 185 LLPSTVNTDLARNVGLKLGDEDHQMQPEDVAELVVDALRLPNRVLLKSATLIMTNPQ 241 >B7DP48.1 B7DP48_9BACL Short-chain dehydrogenase/reductase SDR Length = 241 Score = 283 bits (724), Expect = 8e-75, Method: Compositional matrix adjust. Identities = 140/237 (59%), Positives = 174/237 (73%) Query: 3 LRGRSIVITGAGKGIGKALAMALAKEGANLGLISRTAADLEALKSALTEVYDIKASIAVA 62 L+G S ++TGAGKGIGKA+A LAKEG NLGLI+RT +DL + + Y + A Sbjct: 5 LKGWSAIVTGAGKGIGKAIAEHLAKEGVNLGLIARTESDLNRVADGIRNAYGVSVYTQAA 64 Query: 63 DIAVREDAERAVVSLQKDLGAFDALINNAGIAKFGTFLEMDPADWEHHMQINLFGTYYVT 122 DIA R E A+ L++ LG+ D L+NNAG A FGT ++M +WE +++NL GTYY T Sbjct: 65 DIADRTSIESAIQRLKEQLGSVDVLVNNAGTASFGTVIDMPVEEWERIVRVNLLGTYYAT 124 Query: 123 RAALPAMIERSSGNIINISSTAGERGFATGSAYCASKFALMGMTEALAQEVRKHNIRVVA 182 RA LP MIER+ G+IINISSTAGE+G AT SAY ASKF L+G TE+L EVRK+NIRV+A Sbjct: 125 RAILPHMIERNRGHIINISSTAGEKGSATTSAYSASKFGLLGFTESLMYEVRKYNIRVMA 184 Query: 183 LTPSTVNTGLAENAGLKIGDEDRMMQPEDVAELALAALKLPDRVFLKTAGIWTTNPQ 239 L PSTVNT LA N GLK+GDE+ MQPEDVAEL + AL+LP+RV LK+A + TNPQ Sbjct: 185 LLPSTVNTDLARNVGLKLGDEEHQMQPEDVAELVVDALRLPNRVLLKSATLIMTNPQ 241 >B3ZFI2.1 B3ZFI2_BACCE Oxidoreductase, short-chain dehydrogenase/reductase family Length = 239 Score = 281 bits (720), Expect = 2e-74, Method: Compositional matrix adjust. Identities = 136/236 (57%), Positives = 184/236 (77%), Gaps = 1/236 (0%) Query: 3 LRGRSIVITGAGKGIGKALAMALAKEGANLGLISRTAADLEALKSALTEVYDIKASIAVA 62 L+G++ +ITGAG+GIG+A+A+ALAKEG N+GL++R+ +L+A+ + EV +KA IA A Sbjct: 5 LQGKNALITGAGRGIGRAVAIALAKEGVNVGLLARSEENLKAVAKEV-EVEGVKAVIATA 63 Query: 63 DIAVREDAERAVVSLQKDLGAFDALINNAGIAKFGTFLEMDPADWEHHMQINLFGTYYVT 122 D++ E+ A+ +L+ LG+ D LINNAGI+KFG FLE+D ADWE +Q+NL G YY T Sbjct: 64 DVSSYEEVTTAIETLKNGLGSIDILINNAGISKFGKFLELDVADWEKIIQVNLMGVYYAT 123 Query: 123 RAALPAMIERSSGNIINISSTAGERGFATGSAYCASKFALMGMTEALAQEVRKHNIRVVA 182 RAALP+MIE+ SG+IINISSTAG++G SAY ASKF ++G+TE+LA EVRKHNIRV A Sbjct: 124 RAALPSMIEQQSGDIINISSTAGQKGAPVTSAYSASKFGVLGLTESLAMEVRKHNIRVTA 183 Query: 183 LTPSTVNTGLAENAGLKIGDEDRMMQPEDVAELALAALKLPDRVFLKTAGIWTTNP 238 LTPSTV T +A + GL G+ D++MQ ED+AE +A LKL R F+K+AG+W+TNP Sbjct: 184 LTPSTVATDMAVDLGLTDGNPDKVMQAEDIAEFIVAQLKLNKRTFIKSAGLWSTNP 239 >A7GNV2.1 A7GNV2_BACCN Short-chain dehydrogenase/reductase SDR Length = 239 Score = 281 bits (719), Expect = 3e-74, Method: Compositional matrix adjust. Identities = 136/236 (57%), Positives = 183/236 (77%), Gaps = 1/236 (0%) Query: 3 LRGRSIVITGAGKGIGKALAMALAKEGANLGLISRTAADLEALKSALTEVYDIKASIAVA 62 L+G++ +ITGAG+GIG+A+A+ALAKEG N+GL++R+ +L+A+ + E +KA IA A Sbjct: 5 LQGKNALITGAGRGIGRAVAIALAKEGVNVGLLARSEENLKAVAKEV-EAEGVKAVIATA 63 Query: 63 DIAVREDAERAVVSLQKDLGAFDALINNAGIAKFGTFLEMDPADWEHHMQINLFGTYYVT 122 D++ E+ A+ +L+ LG+ D LINNAGI+KFG FLE+D ADWE +Q+NL G YYVT Sbjct: 64 DVSSYEEVTTAIATLKDGLGSIDILINNAGISKFGKFLELDVADWEKIIQVNLMGVYYVT 123 Query: 123 RAALPAMIERSSGNIINISSTAGERGFATGSAYCASKFALMGMTEALAQEVRKHNIRVVA 182 RAALP+MIE+ SG+IINISSTAG++G SAY ASKF ++G+TE+LA EVRKHNIRV A Sbjct: 124 RAALPSMIEQQSGDIINISSTAGQKGAPVTSAYSASKFGVLGLTESLAMEVRKHNIRVTA 183 Query: 183 LTPSTVNTGLAENAGLKIGDEDRMMQPEDVAELALAALKLPDRVFLKTAGIWTTNP 238 LTPSTV T +A GL G+ D++MQ ED+AE +A LKL R F+K+AG+W+TNP Sbjct: 184 LTPSTVATDMAVELGLTDGNPDKVMQAEDIAEFIVAQLKLNKRTFIKSAGLWSTNP 239 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.132 0.365 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,242,477,136 Number of extensions: 46014196 Number of successful extensions: 263732 Number of sequences better than 1.0e-05: 32926 Number of HSP's gapped: 224133 Number of HSP's successfully gapped: 33019 Length of query: 239 Length of database: 3,060,696,203 Length adjustment: 132 Effective length of query: 107 Effective length of database: 1,817,122,223 Effective search space: 194432077861 Effective search space used: 194432077861 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)