BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0553.0005.p (280 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K7S1.1 B3K7S1_9BACI Uroporphyrinogen III synthase HEM4 293 1e-77 A8FE05.1 A8FE05_BACP2 Uroporphyrinogen-III synthetase EC=4.2.1.75 229 2e-58 B4AFZ3.1 B4AFZ3_BACPU Uroporphyrinogen-III synthase EC=4.2.1.75 228 3e-58 Q9KAX4.1 Q9KAX4_BACHD BH2162 protein 227 9e-58 A4IP29.1 A4IP29_GEOTN Uroporphyrinogen-III synthase 226 2e-57 >B3K7S1.1 B3K7S1_9BACI Uroporphyrinogen III synthase HEM4 Length = 269 Score = 293 bits (750), Expect = 1e-77, Method: Compositional matrix adjust. Identities = 149/267 (55%), Positives = 187/267 (70%) Query: 11 MAEQLQGITVALAGPRKAEEMAKLVQNMGGTALHRPAQGTVFLDNQALREGIAAWIAHPP 70 MA+ ++ VALAGPRKA +MAKLV+ MGGTA+ RPAQGTVFLD++ LR I AW+ P Sbjct: 1 MAQHMKDRVVALAGPRKAADMAKLVEKMGGTAVLRPAQGTVFLDDEVLRRSIEAWLEAPA 60 Query: 71 DLAILTTGMGXXXXXXXXXXXXXXXXXXXVLSASPIAARGYKTVNALKKRGLRPEVRDDD 130 AI TTG+G L + IAARGYKT NALKKRGL P VRDDD Sbjct: 61 PWAIFTTGIGLEAIYDTAEQMGLLDTLNETLERTSIAARGYKTANALKKRGLAPVVRDDD 120 Query: 131 GSTAGLLRGLEDWDLRGKSVVLQLHGDPAPELAAWLKEAGASVLQVEPYRHTPPEPGALA 190 GST+GL+R L DL G+ V+LQLHG+ AP+L WL++ GA V Q+ PY+H PP ++ Sbjct: 121 GSTSGLIRSLAPHDLSGQRVILQLHGESAPKLVDWLQQRGAEVQQILPYKHIPPLEEDIS 180 Query: 191 RLYEEIAEGRVDAVAFTSAPQFRFLAQYAREQGKLKELVEAFEHKVLAVSVGRITSESLK 250 L +EI G+V AVAFTSAPQFRFLA YAR GKL+ ++EAF V+A +VG++T++ L+ Sbjct: 181 LLVDEIVGGKVHAVAFTSAPQFRFLADYARAHGKLEAVLEAFRGPVVAAAVGKVTAQGLR 240 Query: 251 EEGITRIVMPEHERMGSMFVELGKYIA 277 EEGI R+V+PE ERMGSM V LG+Y A Sbjct: 241 EEGIERMVVPEEERMGSMMVTLGRYFA 267 >A8FE05.1 A8FE05_BACP2 Uroporphyrinogen-III synthetase EC=4.2.1.75 Length = 273 Score = 229 bits (584), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 121/266 (45%), Positives = 167/266 (62%) Query: 11 MAEQLQGITVALAGPRKAEEMAKLVQNMGGTALHRPAQGTVFLDNQALREGIAAWIAHPP 70 M++ L G T+A+ G RK EEM LV+ GG A+ R QGTVFL + L+ GI ++ Sbjct: 1 MSKGLSGKTIAICGTRKTEEMRTLVEKQGGQAVIRSLQGTVFLAKEELKPGIETFVKKGA 60 Query: 71 DLAILTTGMGXXXXXXXXXXXXXXXXXXXVLSASPIAARGYKTVNALKKRGLRPEVRDDD 130 D +LTTG+G +LS + IA+RGYKT ALKKRG++P+V D+D Sbjct: 61 DWVVLTTGIGTDTLIESAEELDLGEEFMHILSNAQIASRGYKTYAALKKRGIQPDVSDED 120 Query: 131 GSTAGLLRGLEDWDLRGKSVVLQLHGDPAPELAAWLKEAGASVLQVEPYRHTPPEPGALA 190 G+ L+ LE + +GK V++QLHG+ AP L +L E GA VL + PY+HTPPEP A Sbjct: 121 GTVRDLIAKLEGKEFQGKRVMVQLHGENAPALIQFLHEKGADVLPLLPYQHTPPEPKAAE 180 Query: 191 RLYEEIAEGRVDAVAFTSAPQFRFLAQYAREQGKLKELVEAFEHKVLAVSVGRITSESLK 250 L +E+ +G V AV FT+A Q A + G+ +EL FE VLAV+VG++T+E+LK Sbjct: 181 TLLQEMKDGNVHAVCFTTAVQVHAFFTLAAKWGRKEELQALFEQHVLAVAVGKVTAEALK 240 Query: 251 EEGITRIVMPEHERMGSMFVELGKYI 276 EEGI RI+ P ERMG+M +EL +Y+ Sbjct: 241 EEGIDRILTPSLERMGAMIMELSQYM 266 >B4AFZ3.1 B4AFZ3_BACPU Uroporphyrinogen-III synthase EC=4.2.1.75 Length = 273 Score = 228 bits (582), Expect = 3e-58, Method: Compositional matrix adjust. Identities = 120/266 (45%), Positives = 168/266 (63%) Query: 11 MAEQLQGITVALAGPRKAEEMAKLVQNMGGTALHRPAQGTVFLDNQALREGIAAWIAHPP 70 M++ L G T+A+ G RK EEM LV+ GG A+ R QGTVFL + L+ GI ++ Sbjct: 1 MSKGLAGKTIAICGTRKTEEMRTLVEKQGGQAVIRSLQGTVFLAKEELKPGIETFVKKGA 60 Query: 71 DLAILTTGMGXXXXXXXXXXXXXXXXXXXVLSASPIAARGYKTVNALKKRGLRPEVRDDD 130 D ILTTG+G +LS + IA+RGYKT ALKKRG++P+V D+D Sbjct: 61 DWVILTTGIGTDTLIESAEELHLGEAFMHILSNAQIASRGYKTYAALKKRGIQPDVSDED 120 Query: 131 GSTAGLLRGLEDWDLRGKSVVLQLHGDPAPELAAWLKEAGASVLQVEPYRHTPPEPGALA 190 G+ L+ LE + + K V++QLHG+ AP L +L + GA VLQ+ PY+HTPPEP A Sbjct: 121 GTVRDLISKLEGKEFQDKRVMVQLHGENAPALMKFLHDKGADVLQLLPYQHTPPEPEAAE 180 Query: 191 RLYEEIAEGRVDAVAFTSAPQFRFLAQYAREQGKLKELVEAFEHKVLAVSVGRITSESLK 250 L +E+ +G+V AV FT+A Q A + + +EL FEH VLAV+VG++T+E+LK Sbjct: 181 TLLQEMKDGKVHAVCFTTAVQVHAFFTLAAKWERKEELQALFEHHVLAVAVGKVTAEALK 240 Query: 251 EEGITRIVMPEHERMGSMFVELGKYI 276 EEGI R++ P ERMG+M +EL +Y+ Sbjct: 241 EEGIDRLLAPSLERMGAMIMELSQYM 266 >Q9KAX4.1 Q9KAX4_BACHD BH2162 protein Length = 272 Score = 227 bits (578), Expect = 9e-58, Method: Compositional matrix adjust. Identities = 120/267 (44%), Positives = 170/267 (63%), Gaps = 2/267 (0%) Query: 11 MAEQLQGITVALAGPRKAEEMAKLVQNMGGTALHRPAQGTVF-LDNQALREGIAAWIAHP 69 M L G V LAG RK +EM+ L++ GGT L R QGTVF +D++ L++ + +I+ Sbjct: 1 MGNALSGYKVVLAGTRKTDEMSTLIEKQGGTPLVRSLQGTVFRVDDEVLKD-LETFISKG 59 Query: 70 PDLAILTTGMGXXXXXXXXXXXXXXXXXXXVLSASPIAARGYKTVNALKKRGLRPEVRDD 129 D AILTTG+G L + IAARGYKT N +K+ GL+ EV D+ Sbjct: 60 ADWAILTTGIGTNTLVELAERIGKKDEFLARLKEANIAARGYKTKNVIKQLGLQAEVVDN 119 Query: 130 DGSTAGLLRGLEDWDLRGKSVVLQLHGDPAPELAAWLKEAGASVLQVEPYRHTPPEPGAL 189 DG+ +GL+ +E D+ GK V++QLHG+ AP+L A+L+E GA + + PYRHTP + + Sbjct: 120 DGTVSGLVAAMEGADVTGKRVMVQLHGENAPKLLAFLEEKGADIQTLLPYRHTPAKEETM 179 Query: 190 ARLYEEIAEGRVDAVAFTSAPQFRFLAQYAREQGKLKELVEAFEHKVLAVSVGRITSESL 249 A+ ++EI E DAV FT+A Q R L YA++ LVEAF+ V+AVSVG++T+E+L Sbjct: 180 AQFFQEIEERAFDAVCFTAAIQVRELFTYAKKNNMKDLLVEAFQRHVIAVSVGKVTTEAL 239 Query: 250 KEEGITRIVMPEHERMGSMFVELGKYI 276 ++EGI RIV P+ ERMG+M V L Y+ Sbjct: 240 QDEGIKRIVAPKLERMGAMIVALASYV 266 >A4IP29.1 A4IP29_GEOTN Uroporphyrinogen-III synthase Length = 264 Score = 226 bits (575), Expect = 2e-57, Method: Compositional matrix adjust. Identities = 117/262 (44%), Positives = 156/262 (59%) Query: 15 LQGITVALAGPRKAEEMAKLVQNMGGTALHRPAQGTVFLDNQALREGIAAWIAHPPDLAI 74 ++G VAL RK +EM L++ GGTA+ RPAQGTVFL L E + A I PD + Sbjct: 1 MRGKRVALCASRKLDEMTTLIEKQGGTAVIRPAQGTVFLKGSELSEEMRAIIMMRPDWIV 60 Query: 75 LTTGMGXXXXXXXXXXXXXXXXXXXVLSASPIAARGYKTVNALKKRGLRPEVRDDDGSTA 134 TTG+G V+ + IAARGYKTV ALK G+ P +DG+T Sbjct: 61 FTTGIGVEALWKTAEREGIAVEWKRVIENADIAARGYKTVAALKNIGVIPTAVSEDGTTK 120 Query: 135 GLLRGLEDWDLRGKSVVLQLHGDPAPELAAWLKEAGASVLQVEPYRHTPPEPGALARLYE 194 GL+ L +D GKSVV QL+GD AP L + GAS ++ PYRH P+ L LY Sbjct: 121 GLISALNAYDFAGKSVVAQLYGDTAPVLKQFFLTRGASYTELFPYRHVAPDHEMLEMLYN 180 Query: 195 EIAEGRVDAVAFTSAPQFRFLAQYAREQGKLKELVEAFEHKVLAVSVGRITSESLKEEGI 254 E+ +DAV FTSA Q RF +ARE+ + L+EAF V+A +VG++T E+L+EEG+ Sbjct: 181 EVVGREIDAVCFTSAIQVRFFFSFAREKQDITPLLEAFCTDVVACAVGKVTKEALEEEGV 240 Query: 255 TRIVMPEHERMGSMFVELGKYI 276 +R++ PEHERMG+M V L +Y Sbjct: 241 SRVITPEHERMGAMIVTLARYF 262 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.133 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,575,189,036 Number of extensions: 61264201 Number of successful extensions: 171436 Number of sequences better than 1.0e-05: 127 Number of HSP's gapped: 171269 Number of HSP's successfully gapped: 127 Length of query: 280 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 146 Effective length of database: 1,798,280,193 Effective search space: 262548908178 Effective search space used: 262548908178 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 130 (54.7 bits)