BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0551.0004.p (361 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1D4Z2.1 B1D4Z2_9BACL Glyoxalase/bleomycin resistance protein/di... 402 e-110 B1DCY2.1 B1DCY2_9BACL Glyoxalase/bleomycin resistance protein/di... 399 e-109 P96693.1 MHQO_BACSU Putative ring-cleaving dioxygenase mhqO EC=1... 397 e-109 C0ZIT7.1 C0ZIT7_BREBN Putative ring-cleaving dioxygenase MhqO 395 e-108 A7Z111.1 A7Z111_BACA2 YdfO 394 e-108 >B1D4Z2.1 B1D4Z2_9BACL Glyoxalase/bleomycin resistance protein/dioxygenase Length = 319 Score = 402 bits (1034), Expect = e-110, Method: Compositional matrix adjust. Identities = 192/312 (61%), Positives = 233/312 (74%) Query: 46 MTMQTQGIHHITAFVGDVQRNADFYAGILGLRLVKKTINFDAPEVYHLYFGNEQGSPGTI 105 +TM+T GIHHIT FV D QRN DFYAG+LGLRLVKKTINFDAPEVYHLYFGNE+GSPGTI Sbjct: 4 ITMKTAGIHHITCFVKDAQRNVDFYAGVLGLRLVKKTINFDAPEVYHLYFGNEKGSPGTI 63 Query: 106 ITFFPWSXXXXXXXXXXXXXXTTYAVPVGSMDFWKQRLAAYQIPVTEVSRIGESYLAFAD 165 ITFFP+ TTY VP+G+++FW+ RLAA+ I E +R E+Y+ F+D Sbjct: 64 ITFFPYEFSHKGRIGGGQVGTTTYVVPLGALEFWRARLAAFDISFKESTRFSENYIEFSD 123 Query: 166 FDGLRIELVEREEGALSTWSFGGVTPEHAIKGFGGAVLYSTNPEKTADTLSRTLGLERIA 225 DGLR+E+VERE+GAL+TWSFGG+ + AIKGFGGAVLYST T L LGLE++ Sbjct: 124 LDGLRLEIVEREDGALNTWSFGGIPTDKAIKGFGGAVLYSTASIDTMKVLEDVLGLEKVG 183 Query: 226 EGDGYIRYRSTGDLGNIIDLKAAPVPHGAGGSGTVHHIAWRAKDDAEQLEWGRRVQSHGY 285 G+IRY++ G+LGN+ID+ A P+ G G+G VHHIAWRAK+ E W V+S G+ Sbjct: 184 LEGGFIRYKAEGELGNLIDVNAEPMEAGIPGAGMVHHIAWRAKNFEEHDAWRSHVESSGF 243 Query: 286 QPTPVQDRQYFNAIYFREEGGILFEIATDPPGFARDEAPDALGQKLMLPAWFEPHRAVIE 345 PTPV DRQYF+AIYFRE GGILFEIATDPPGFA DE D LG+ LMLP WFE RA IE Sbjct: 244 HPTPVIDRQYFHAIYFREHGGILFEIATDPPGFANDEHEDHLGEALMLPLWFENSRAEIE 303 Query: 346 ENLTPFEIREIE 357 +NL P +++E+E Sbjct: 304 KNLPPIQVQELE 315 >B1DCY2.1 B1DCY2_9BACL Glyoxalase/bleomycin resistance protein/dioxygenase Length = 316 Score = 399 bits (1025), Expect = e-109, Method: Compositional matrix adjust. Identities = 201/316 (63%), Positives = 242/316 (76%), Gaps = 1/316 (0%) Query: 46 MTMQ-TQGIHHITAFVGDVQRNADFYAGILGLRLVKKTINFDAPEVYHLYFGNEQGSPGT 104 MT Q T GIHHITAFV Q DFYAG+LGLRLVKKTINFDAPEVYHLYFG++ GSPGT Sbjct: 1 MTNQRTAGIHHITAFVKHPQVTTDFYAGVLGLRLVKKTINFDAPEVYHLYFGDQAGSPGT 60 Query: 105 IITFFPWSXXXXXXXXXXXXXXTTYAVPVGSMDFWKQRLAAYQIPVTEVSRIGESYLAFA 164 IITFFPW TTY VP G++ FWK+RLA ++I E SR GE YL FA Sbjct: 61 IITFFPWENSRKGVVGGGQVGYTTYVVPEGTLGFWKERLAKFRIEFNETSRFGEQYLQFA 120 Query: 165 DFDGLRIELVEREEGALSTWSFGGVTPEHAIKGFGGAVLYSTNPEKTADTLSRTLGLERI 224 D DGL +ELV REEG STWSFGGV+ ++AIKGFGGA+LYSTNP TA L +GL ++ Sbjct: 121 DPDGLLVELVAREEGPRSTWSFGGVSADNAIKGFGGAILYSTNPAATAQLLEHVMGLSKV 180 Query: 225 AEGDGYIRYRSTGDLGNIIDLKAAPVPHGAGGSGTVHHIAWRAKDDAEQLEWGRRVQSHG 284 E +G RY+S G+LGNIID+ P+ G GG GTVHHIAWRAKDDAE L W + + ++G Sbjct: 181 DEENGLARYQSFGELGNIIDVNTEPMAAGHGGHGTVHHIAWRAKDDAEHLTWQQHIAANG 240 Query: 285 YQPTPVQDRQYFNAIYFREEGGILFEIATDPPGFARDEAPDALGQKLMLPAWFEPHRAVI 344 +QPTP+ DRQYFNA+YFRE+GGILFEIATDPPGFARDE P+ LG++LMLPAW+E HRA + Sbjct: 241 FQPTPIVDRQYFNALYFREQGGILFEIATDPPGFARDEDPEHLGEQLMLPAWYEQHRATL 300 Query: 345 EENLTPFEIREIEVNQ 360 E+ LTPF++R +E+++ Sbjct: 301 EQLLTPFDVRVMELDR 316 >P96693.1 MHQO_BACSU Putative ring-cleaving dioxygenase mhqO EC=1.13.11.- Length = 312 Score = 397 bits (1021), Expect = e-109, Method: Compositional matrix adjust. Identities = 186/312 (59%), Positives = 227/312 (72%) Query: 46 MTMQTQGIHHITAFVGDVQRNADFYAGILGLRLVKKTINFDAPEVYHLYFGNEQGSPGTI 105 M +T GIHHITA VG Q N DFYAG+LGLRLVK+T+NFD P YHLYFGNE G PGTI Sbjct: 1 MAKKTMGIHHITAIVGHPQENTDFYAGVLGLRLVKQTVNFDDPGTYHLYFGNEGGKPGTI 60 Query: 106 ITFFPWSXXXXXXXXXXXXXXTTYAVPVGSMDFWKQRLAAYQIPVTEVSRIGESYLAFAD 165 ITFFPW+ T+Y VP G+M FW++RL + +P T++ R GE Y+ F D Sbjct: 61 ITFFPWAGARQGVIGDGQVGVTSYVVPKGAMAFWEKRLEKFNVPYTKIERFGEQYVEFDD 120 Query: 166 FDGLRIELVEREEGALSTWSFGGVTPEHAIKGFGGAVLYSTNPEKTADTLSRTLGLERIA 225 GL +E+VEREEG +TW+FG VTP+ AIKGFGGA L S P+KTAD L +GLER+ Sbjct: 121 PHGLHLEIVEREEGEANTWTFGEVTPDVAIKGFGGATLLSEQPDKTADLLENIMGLERVG 180 Query: 226 EGDGYIRYRSTGDLGNIIDLKAAPVPHGAGGSGTVHHIAWRAKDDAEQLEWGRRVQSHGY 285 + ++RYRS GD+GN+IDLK P+ G G+GTVHHIAWRA DD +QL+W R + SHGY Sbjct: 181 KEGDFVRYRSAGDIGNVIDLKLTPIGRGQMGAGTVHHIAWRANDDEDQLDWQRYIASHGY 240 Query: 286 QPTPVQDRQYFNAIYFREEGGILFEIATDPPGFARDEAPDALGQKLMLPAWFEPHRAVIE 345 TPV+DR YFNAIYFRE G ILFEIATDPPGFA DE + +G+KLMLP +EPHR IE Sbjct: 241 GVTPVRDRNYFNAIYFREHGEILFEIATDPPGFAHDETQETMGEKLMLPVQYEPHRTQIE 300 Query: 346 ENLTPFEIREIE 357 + L PFE+RE++ Sbjct: 301 QGLLPFEVRELD 312 >C0ZIT7.1 C0ZIT7_BREBN Putative ring-cleaving dioxygenase MhqO Length = 315 Score = 395 bits (1014), Expect = e-108, Method: Compositional matrix adjust. Identities = 189/315 (60%), Positives = 224/315 (71%) Query: 46 MTMQTQGIHHITAFVGDVQRNADFYAGILGLRLVKKTINFDAPEVYHLYFGNEQGSPGTI 105 MT QT GIHHITAFV Q N DFYAG LGLRLVKKTINFDAP+VYHLYFGNE GSPGT Sbjct: 1 MTQQTAGIHHITAFVTSAQNNVDFYAGFLGLRLVKKTINFDAPDVYHLYFGNEVGSPGTA 60 Query: 106 ITFFPWSXXXXXXXXXXXXXXTTYAVPVGSMDFWKQRLAAYQIPVTEVSRIGESYLAFAD 165 ITFFPW TT+ +PVG+ FW+ RL + I + V R E+YL F D Sbjct: 61 ITFFPWETGRRGRVGGGQVGYTTFLIPVGAFSFWEDRLKKFGIEYSRVERFNETYLQFTD 120 Query: 166 FDGLRIELVEREEGALSTWSFGGVTPEHAIKGFGGAVLYSTNPEKTADTLSRTLGLERIA 225 DGL++E+VERE G S WSFGGV + AIKGFGGA+LYS P T D L R +GL R+ Sbjct: 121 RDGLQLEIVEREGGPASKWSFGGVPADKAIKGFGGAILYSMAPAHTMDVLERLMGLTRVG 180 Query: 226 EGDGYIRYRSTGDLGNIIDLKAAPVPHGAGGSGTVHHIAWRAKDDAEQLEWGRRVQSHGY 285 E +G +R+++ GDLGN+ID+ A P+P GAGG+GT+HHIAWRA DD + W +R G Sbjct: 181 EENGLVRFKAHGDLGNVIDVNAEPIPRGAGGAGTIHHIAWRAVDDQDHQLWQQRAAQSGL 240 Query: 286 QPTPVQDRQYFNAIYFREEGGILFEIATDPPGFARDEAPDALGQKLMLPAWFEPHRAVIE 345 PT + DRQYFNAIYFRE G ILFEIATDPPGF RDE +ALGQKLMLP W+EP RA IE Sbjct: 241 HPTDIIDRQYFNAIYFREPGEILFEIATDPPGFERDEPFEALGQKLMLPEWYEPRRAQIE 300 Query: 346 ENLTPFEIREIEVNQ 360 + LTP +R +E ++ Sbjct: 301 QGLTPITVRVLEEDK 315 >A7Z111.1 A7Z111_BACA2 YdfO Length = 312 Score = 394 bits (1013), Expect = e-108, Method: Compositional matrix adjust. Identities = 186/312 (59%), Positives = 227/312 (72%) Query: 46 MTMQTQGIHHITAFVGDVQRNADFYAGILGLRLVKKTINFDAPEVYHLYFGNEQGSPGTI 105 M +T GIHHITA VG Q NADFYAG+LGLRLVK+T+NFD P YHLYFG+E G PGTI Sbjct: 1 MAKKTMGIHHITAIVGHPQENADFYAGVLGLRLVKQTVNFDDPGTYHLYFGDEGGKPGTI 60 Query: 106 ITFFPWSXXXXXXXXXXXXXXTTYAVPVGSMDFWKQRLAAYQIPVTEVSRIGESYLAFAD 165 ITFFPW+ T+YAVP G+M FW++RL + IP T++ R GE Y+ F D Sbjct: 61 ITFFPWAGARQGIIGDGQVGVTSYAVPKGAMAFWEKRLEKFNIPYTKIERFGEQYIEFDD 120 Query: 166 FDGLRIELVEREEGALSTWSFGGVTPEHAIKGFGGAVLYSTNPEKTADTLSRTLGLERIA 225 GL +E+VERE G +TW+FG VTP+ AIKGFGGA L S PEKT L +GLER+ Sbjct: 121 PHGLHLEIVEREAGEANTWTFGDVTPDVAIKGFGGATLLSAQPEKTGKLLENIMGLERVG 180 Query: 226 EGDGYIRYRSTGDLGNIIDLKAAPVPHGAGGSGTVHHIAWRAKDDAEQLEWGRRVQSHGY 285 + ++RYRS GD+GN+IDLK P+ G G GTVHHIAWRA+DD +QL+W R + +HGY Sbjct: 181 KEGDFVRYRSAGDIGNVIDLKLTPIGRGQMGVGTVHHIAWRAQDDEDQLDWQRFIAAHGY 240 Query: 286 QPTPVQDRQYFNAIYFREEGGILFEIATDPPGFARDEAPDALGQKLMLPAWFEPHRAVIE 345 TPVQDR YFNA+YFRE G ILFEIATDPPGFA DE+ + +G KLMLP +EPHRA IE Sbjct: 241 GVTPVQDRNYFNAVYFREHGEILFEIATDPPGFAHDESQETMGGKLMLPEQYEPHRAQIE 300 Query: 346 ENLTPFEIREIE 357 + L PFE+RE++ Sbjct: 301 QGLLPFEVRELD 312 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.139 0.429 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,313,527,793 Number of extensions: 102342005 Number of successful extensions: 185782 Number of sequences better than 1.0e-05: 544 Number of HSP's gapped: 184647 Number of HSP's successfully gapped: 554 Length of query: 361 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 224 Effective length of database: 1,770,017,148 Effective search space: 396483841152 Effective search space used: 396483841152 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)