BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0548.0040.p (401 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C2NZL0.1 C2NZL0_BACCE Glycosyltransferase 557 e-157 B5UUR4.1 B5UUR4_BACCE Macrolide glycosyltransferase 555 e-156 Q81CG5.1 Q81CG5_BACCR Macrolide glycosyltransferase EC=2.4.1.- 555 e-156 C2T223.1 C2T223_BACCE Glycosyltransferase 555 e-156 C2RNR0.1 C2RNR0_BACCE Glycosyltransferase 555 e-156 >C2NZL0.1 C2NZL0_BACCE Glycosyltransferase Length = 400 Score = 557 bits (1435), Expect = e-157, Method: Compositional matrix adjust. Identities = 270/398 (67%), Positives = 313/398 (78%), Gaps = 1/398 (0%) Query: 4 MARVLFINGGSEGHINPTIGVVQELISRGEEVVYFCIEAYRERIEKTGASVRTIDDQKII 63 MARVLFIN GSEGHINPT+ VV+ELISRGEEVVYF IEA+RERIEKTGA+VRTIDDQK I Sbjct: 4 MARVLFINAGSEGHINPTLQVVEELISRGEEVVYFSIEAFRERIEKTGATVRTIDDQKFI 63 Query: 64 NAFIXXXXRNYLPERINGLLLTADIIIPSVLEQIAGEHFDYIIHDSMFGCGRMLAQIFEL 123 AF+ RNYL ERINGLL TAD++IPSVLEQI GEHFDYIIHDSMFGCG ++AQI +L Sbjct: 64 KAFLSGG-RNYLQERINGLLHTADVVIPSVLEQIEGEHFDYIIHDSMFGCGHLIAQILKL 122 Query: 124 PAINSSTSFAQTQASFDKMLEQPSMKIPAEIAKPIYDRYHSLTAMLKEKYKVEIHSPYEV 183 P+INS TSFAQ + SF++ML S IP EI I + + +LT + EKY VEI SPYEV Sbjct: 123 PSINSCTSFAQDEKSFEQMLGHLSKNIPVEIHDKIQNDFQNLTKGIAEKYGVEIKSPYEV 182 Query: 184 LCNPAPLTIVYTTREFQPDGDAFDQTYKFVGPSLSSRNTQAHFDFSAIQGKNPIYISLGT 243 CNPAPLTIVYT +EFQP GD FD+TYKFVGPS+S++ DF++IQ K+PIYISLGT Sbjct: 183 FCNPAPLTIVYTIKEFQPFGDTFDETYKFVGPSISAQVKNRDVDFTSIQEKSPIYISLGT 242 Query: 244 VFNQAMDFYKLCFEAFGNTDHTIVMSVGNKTPISDLGEIPKNFIVQNYVPQTELLKQISL 303 VFN+A+DFYKLC +AF N++HTIVMS+GNKT ISDLGEIPKNFIV+NYVPQTELL L Sbjct: 243 VFNEAIDFYKLCMKAFENSEHTIVMSIGNKTKISDLGEIPKNFIVKNYVPQTELLTYTKL 302 Query: 304 FITHGGMNSTHEALYYGVPLVVIPQSADQPXXXXXXXXXXXXXXLQMQSLTANGLREAAE 363 FITHGGMNS HE LY G+PLVVIPQSADQP L M LT LRE+ E Sbjct: 303 FITHGGMNSVHEGLYNGIPLVVIPQSADQPAIAKQVESLGAGVQLHMNELTVEKLRESVE 362 Query: 364 QVLNHPSYKKAVDNMKASLQRSGGYRQAADEIIAFLGR 401 VLN+PSYK+A N+K S + SGGY++A DEI F+G+ Sbjct: 363 LVLNNPSYKEAALNLKESFRTSGGYKEAVDEIFTFVGQ 400 >B5UUR4.1 B5UUR4_BACCE Macrolide glycosyltransferase Length = 397 Score = 555 bits (1429), Expect = e-156, Method: Compositional matrix adjust. Identities = 268/398 (67%), Positives = 313/398 (78%), Gaps = 1/398 (0%) Query: 4 MARVLFINGGSEGHINPTIGVVQELISRGEEVVYFCIEAYRERIEKTGASVRTIDDQKII 63 MARVLFIN GSEGHINPT+ VV+ELISRGEEVVYF IEA+R+RIEKTGA+VRTIDDQK I Sbjct: 1 MARVLFINAGSEGHINPTLQVVEELISRGEEVVYFSIEAFRDRIEKTGATVRTIDDQKFI 60 Query: 64 NAFIXXXXRNYLPERINGLLLTADIIIPSVLEQIAGEHFDYIIHDSMFGCGRMLAQIFEL 123 AF+ RNYL ERINGLL TAD++IPSVLEQI GEHFDYIIHDSMFGCG ++AQI +L Sbjct: 61 KAFLSGG-RNYLQERINGLLHTADVVIPSVLEQIEGEHFDYIIHDSMFGCGHLIAQILKL 119 Query: 124 PAINSSTSFAQTQASFDKMLEQPSMKIPAEIAKPIYDRYHSLTAMLKEKYKVEIHSPYEV 183 P+INS TSFAQ + SF++ML S IP EI I + + +LT + EKY VEI SPYEV Sbjct: 120 PSINSCTSFAQDEKSFEQMLGHLSKNIPVEIHDKIQNDFQNLTKGIAEKYGVEIKSPYEV 179 Query: 184 LCNPAPLTIVYTTREFQPDGDAFDQTYKFVGPSLSSRNTQAHFDFSAIQGKNPIYISLGT 243 CNPAPLTIVYT +EFQP GD FD+TYKFVGPS+S++ DF++I+ K+PIYISLGT Sbjct: 180 FCNPAPLTIVYTIKEFQPFGDTFDETYKFVGPSISAQVKNRDVDFTSIEEKSPIYISLGT 239 Query: 244 VFNQAMDFYKLCFEAFGNTDHTIVMSVGNKTPISDLGEIPKNFIVQNYVPQTELLKQISL 303 VFN+A+DFYKLC +AF N++HTIVMS+GNKT ISDLGEIPKNFIV+NYVPQTELL L Sbjct: 240 VFNEAIDFYKLCMKAFENSEHTIVMSIGNKTKISDLGEIPKNFIVKNYVPQTELLTYTKL 299 Query: 304 FITHGGMNSTHEALYYGVPLVVIPQSADQPXXXXXXXXXXXXXXLQMQSLTANGLREAAE 363 FITHGGMNS HE LY G+PLVVIPQSADQP L M LT LRE+ E Sbjct: 300 FITHGGMNSVHEGLYNGIPLVVIPQSADQPAIAKQVESLGAGVQLHMNELTVEKLRESVE 359 Query: 364 QVLNHPSYKKAVDNMKASLQRSGGYRQAADEIIAFLGR 401 VLN+PSYK+A N+K S + SGGY++A DEI F+G+ Sbjct: 360 LVLNNPSYKEAALNLKESFRTSGGYKEAVDEIFTFVGQ 397 >Q81CG5.1 Q81CG5_BACCR Macrolide glycosyltransferase EC=2.4.1.- Length = 397 Score = 555 bits (1429), Expect = e-156, Method: Compositional matrix adjust. Identities = 270/398 (67%), Positives = 313/398 (78%), Gaps = 1/398 (0%) Query: 4 MARVLFINGGSEGHINPTIGVVQELISRGEEVVYFCIEAYRERIEKTGASVRTIDDQKII 63 MARVLFIN GSEGHINPT+ VV ELISRGEEVVYF IEA+RERIEKTGA+VRTIDDQK I Sbjct: 1 MARVLFINAGSEGHINPTLQVVDELISRGEEVVYFSIEAFRERIEKTGATVRTIDDQKFI 60 Query: 64 NAFIXXXXRNYLPERINGLLLTADIIIPSVLEQIAGEHFDYIIHDSMFGCGRMLAQIFEL 123 AF+ RNYL ERINGLL TADI+IPSVLEQI GEHFDYIIHDSMFGCG ++AQI +L Sbjct: 61 KAFLSGG-RNYLQERINGLLHTADIVIPSVLEQIEGEHFDYIIHDSMFGCGHLIAQILKL 119 Query: 124 PAINSSTSFAQTQASFDKMLEQPSMKIPAEIAKPIYDRYHSLTAMLKEKYKVEIHSPYEV 183 PAINS TSFAQ + SF++ML S IP EI I + + +LT + EKY VEI S YEV Sbjct: 120 PAINSCTSFAQDEKSFEQMLGHLSKNIPVEIYDKIQNDFQNLTKGIAEKYGVEIKSSYEV 179 Query: 184 LCNPAPLTIVYTTREFQPDGDAFDQTYKFVGPSLSSRNTQAHFDFSAIQGKNPIYISLGT 243 CNPAPLTIVYT +EFQP GD FD+ YKFVGPS+S++ DF++I+ K+PIYISLGT Sbjct: 180 FCNPAPLTIVYTIKEFQPFGDTFDEIYKFVGPSISAQMKNRDVDFTSIEEKSPIYISLGT 239 Query: 244 VFNQAMDFYKLCFEAFGNTDHTIVMSVGNKTPISDLGEIPKNFIVQNYVPQTELLKQISL 303 VFN+A+DFYKLC +AF N++HTIVMS+G+KT ISDLGEIPKNFIV+NYVPQTELL L Sbjct: 240 VFNEAIDFYKLCMKAFENSEHTIVMSIGSKTKISDLGEIPKNFIVKNYVPQTELLTYTKL 299 Query: 304 FITHGGMNSTHEALYYGVPLVVIPQSADQPXXXXXXXXXXXXXXLQMQSLTANGLREAAE 363 FITHGGMNS HE LY GVPLVVIPQSADQP LQMQ LTA+ L E+ E Sbjct: 300 FITHGGMNSAHEGLYNGVPLVVIPQSADQPVVAKQVESLGAGIKLQMQGLTADQLSESVE 359 Query: 364 QVLNHPSYKKAVDNMKASLQRSGGYRQAADEIIAFLGR 401 VLN+PS+K+ N+K S Q+SGGY++A DEI F+G+ Sbjct: 360 MVLNNPSFKEVALNLKKSFQKSGGYKEAVDEIFIFVGQ 397 >C2T223.1 C2T223_BACCE Glycosyltransferase Length = 397 Score = 555 bits (1429), Expect = e-156, Method: Compositional matrix adjust. Identities = 270/398 (67%), Positives = 313/398 (78%), Gaps = 1/398 (0%) Query: 4 MARVLFINGGSEGHINPTIGVVQELISRGEEVVYFCIEAYRERIEKTGASVRTIDDQKII 63 MARVLFIN GSEGHINPT+ VV ELISRGEEVVYF IEA+RERIEKTGA+VRTIDDQK I Sbjct: 1 MARVLFINAGSEGHINPTLQVVDELISRGEEVVYFSIEAFRERIEKTGATVRTIDDQKFI 60 Query: 64 NAFIXXXXRNYLPERINGLLLTADIIIPSVLEQIAGEHFDYIIHDSMFGCGRMLAQIFEL 123 AF+ RNYL ERINGLL TADI+IPSVLEQI GEHFDYIIHDSMFGCG ++AQI +L Sbjct: 61 KAFLSGG-RNYLQERINGLLHTADIVIPSVLEQIEGEHFDYIIHDSMFGCGHLIAQILKL 119 Query: 124 PAINSSTSFAQTQASFDKMLEQPSMKIPAEIAKPIYDRYHSLTAMLKEKYKVEIHSPYEV 183 PAINS TSFAQ + SF++ML S IP EI I + + +LT + EKY VEI S YEV Sbjct: 120 PAINSCTSFAQDEKSFEQMLGHLSKNIPVEIYDKIQNDFQNLTKGIAEKYGVEIKSSYEV 179 Query: 184 LCNPAPLTIVYTTREFQPDGDAFDQTYKFVGPSLSSRNTQAHFDFSAIQGKNPIYISLGT 243 CNPAPLTIVYT +EFQP GD FD+ YKFVGPS+S++ DF++I+ K+PIYISLGT Sbjct: 180 FCNPAPLTIVYTIKEFQPFGDTFDEIYKFVGPSISAQMKNRDVDFTSIEEKSPIYISLGT 239 Query: 244 VFNQAMDFYKLCFEAFGNTDHTIVMSVGNKTPISDLGEIPKNFIVQNYVPQTELLKQISL 303 VFN+A+DFYKLC +AF N++HTIVMS+G+KT ISDLGEIPKNFIV+NYVPQTELL L Sbjct: 240 VFNEAIDFYKLCMKAFENSEHTIVMSIGSKTKISDLGEIPKNFIVKNYVPQTELLTYTKL 299 Query: 304 FITHGGMNSTHEALYYGVPLVVIPQSADQPXXXXXXXXXXXXXXLQMQSLTANGLREAAE 363 FITHGGMNS HE LY GVPLVVIPQSADQP LQMQ LTA+ L E+ E Sbjct: 300 FITHGGMNSAHEGLYNGVPLVVIPQSADQPVVAKQVESLGAGIKLQMQGLTADQLSESVE 359 Query: 364 QVLNHPSYKKAVDNMKASLQRSGGYRQAADEIIAFLGR 401 VLN+PS+K+ N+K S Q+SGGY++A DEI F+G+ Sbjct: 360 MVLNNPSFKEVALNLKKSFQKSGGYKEAVDEIFIFVGQ 397 >C2RNR0.1 C2RNR0_BACCE Glycosyltransferase Length = 397 Score = 555 bits (1429), Expect = e-156, Method: Compositional matrix adjust. Identities = 270/398 (67%), Positives = 313/398 (78%), Gaps = 1/398 (0%) Query: 4 MARVLFINGGSEGHINPTIGVVQELISRGEEVVYFCIEAYRERIEKTGASVRTIDDQKII 63 MARVLFIN GSEGHINPT+ VV ELISRGEEVVYF IEA+RERIEKTGA+VRTIDDQK I Sbjct: 1 MARVLFINAGSEGHINPTLQVVDELISRGEEVVYFSIEAFRERIEKTGATVRTIDDQKFI 60 Query: 64 NAFIXXXXRNYLPERINGLLLTADIIIPSVLEQIAGEHFDYIIHDSMFGCGRMLAQIFEL 123 AF+ RNYL ERINGLL TADI+IPSVLEQI GEHFDYIIHDSMFGCG ++AQI +L Sbjct: 61 KAFLSGG-RNYLQERINGLLHTADIVIPSVLEQIEGEHFDYIIHDSMFGCGHLIAQILKL 119 Query: 124 PAINSSTSFAQTQASFDKMLEQPSMKIPAEIAKPIYDRYHSLTAMLKEKYKVEIHSPYEV 183 PAINS TSFAQ + SF++ML S IP EI I + + +LT + EKY VEI S YEV Sbjct: 120 PAINSCTSFAQDEKSFEQMLGHLSKNIPVEIYDKIQNDFQNLTKGIAEKYGVEIKSSYEV 179 Query: 184 LCNPAPLTIVYTTREFQPDGDAFDQTYKFVGPSLSSRNTQAHFDFSAIQGKNPIYISLGT 243 CNPAPLTIVYT +EFQP GD FD+ YKFVGPS+S++ DF++I+ K+PIYISLGT Sbjct: 180 FCNPAPLTIVYTIKEFQPFGDTFDEIYKFVGPSISAQMKNRDVDFTSIEEKSPIYISLGT 239 Query: 244 VFNQAMDFYKLCFEAFGNTDHTIVMSVGNKTPISDLGEIPKNFIVQNYVPQTELLKQISL 303 VFN+A+DFYKLC +AF N++HTIVMS+G+KT ISDLGEIPKNFIV+NYVPQTELL L Sbjct: 240 VFNEAIDFYKLCMKAFENSEHTIVMSIGSKTKISDLGEIPKNFIVKNYVPQTELLTYTKL 299 Query: 304 FITHGGMNSTHEALYYGVPLVVIPQSADQPXXXXXXXXXXXXXXLQMQSLTANGLREAAE 363 FITHGGMNS HE LY GVPLVVIPQSADQP LQMQ LTA+ L E+ E Sbjct: 300 FITHGGMNSAHEGLYNGVPLVVIPQSADQPVVAKQVESLGAGIKLQMQGLTADQLSESVE 359 Query: 364 QVLNHPSYKKAVDNMKASLQRSGGYRQAADEIIAFLGR 401 VLN+PS+K+ N+K S Q+SGGY++A DEI F+G+ Sbjct: 360 MVLNNPSFKEVALNLKKSFQKSGGYKEAVDEIFIFVGQ 397 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.136 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,187,967,854 Number of extensions: 86440116 Number of successful extensions: 211930 Number of sequences better than 1.0e-05: 947 Number of HSP's gapped: 211853 Number of HSP's successfully gapped: 951 Length of query: 401 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 263 Effective length of database: 1,760,596,133 Effective search space: 463036782979 Effective search space used: 463036782979 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)