BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0548.0038.p (225 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1D599.1 B1D599_9BACL S-layer domain protein 68 8e-10 B7GKY4.1 B7GKY4_ANOFW Alpha-amylase/pullulanase 66 3e-09 B3K3B9.1 B3K3B9_9BACI S-layer domain protein 65 4e-09 B1DD74.1 B1DD74_9BACL S-layer domain protein 65 4e-09 B1D969.1 B1D969_9BACL Cellulosome anchoring protein cohesin region 65 5e-09 >B1D599.1 B1D599_9BACL S-layer domain protein Length = 1682 Score = 67.8 bits (164), Expect = 8e-10, Method: Compositional matrix adjust. Identities = 55/180 (30%), Positives = 82/180 (45%), Gaps = 9/180 (5%) Query: 42 TFTDAKQVAVWARSDMAAALSNGYIQGVSASGNVLAPKQKXXXXXXXXXXXXXXXXXPED 101 +F D VA WA + A + G IQG A G V P+ P+ Sbjct: 1504 SFNDLSGVA-WASEAIDALAAKGVIQG-KAEG-VFDPRSSITRAEFITLLMRALEL-PQA 1559 Query: 102 KAVSGFPDVKPGKWYSGTVAKAVELGIVSRS-SGKFEPDRGITREEAAVMVAKAA----R 156 A +GF DV +Y +A A GIV+ + +FEP++ ITRE+ VM A+A + Sbjct: 1560 AAAAGFGDVSESAYYYNALASAKAYGIVTGTGDNRFEPNKQITREDTFVMTARALDALHQ 1619 Query: 157 LSTYGSAGRVKFGDTSTLPQDSRQAIQAVNEHEIMTGDGGSFHPKQVLVREQAAAILVRV 216 L + F D + ++ +I A++ I+ GD HPK L R +AA ++ RV Sbjct: 1620 LDASSNGDLSSFKDADAISSYAQASISALSAAGIVQGDATGIHPKSNLTRAEAAVMIYRV 1679 >B7GKY4.1 B7GKY4_ANOFW Alpha-amylase/pullulanase Length = 1990 Score = 65.9 bits (159), Expect = 3e-09, Method: Compositional matrix adjust. Identities = 43/119 (36%), Positives = 65/119 (54%), Gaps = 12/119 (10%) Query: 107 FPDVKPGKWYS--GTVAKAVELGIVS-RSSGKFEPDRGITREEAAVMVAKAARLSTYGSA 163 F DVK +W++ G +A AV+ GI+ +++ F PD ITR +AAVM+ +A +LS G Sbjct: 1862 FADVKGTEWFNKNGELASAVKFGIIQGKTAHMFAPDEPITRAQAAVMIERALKLSFVGYD 1921 Query: 164 GRVK--------FGDTSTLPQDSRQAIQAVNEHEIMTG-DGGSFHPKQVLVREQAAAIL 213 K F D LP ++Q I++V + IM G D G+F P + R + A +L Sbjct: 1922 ETAKDKTKKVTDFRDAKQLPTWAKQEIESVYQAGIMQGRDSGNFDPISHMTRAEMAKVL 1980 >B3K3B9.1 B3K3B9_9BACI S-layer domain protein Length = 900 Score = 65.1 bits (157), Expect = 4e-09, Method: Compositional matrix adjust. Identities = 37/109 (33%), Positives = 60/109 (55%), Gaps = 2/109 (1%) Query: 107 FPDVKPGKWYSGTVAKAVELGIVS-RSSGKFEPDRGITREEAAVMVAKAARLSTYGSAGR 165 F D++ W +A+AV+ G + + PD +TREEAAVMVAK L + + Sbjct: 91 FKDLETSHWAYDQIARAVKAGYIDGYNDNTVRPDHTVTREEAAVMVAKLTELVSAETVSA 150 Query: 166 VKFGDTSTLPQDSRQAIQAVNEHEIMTGDG-GSFHPKQVLVREQAAAIL 213 F D S++ + S+ A+ A+ E ++TG G+F+P+ L R +A +L Sbjct: 151 TDFKDDSSISKWSKPAVSALAEKAVITGSAKGNFNPQGKLTRAEAVTLL 199 >B1DD74.1 B1DD74_9BACL S-layer domain protein Length = 536 Score = 65.1 bits (157), Expect = 4e-09, Method: Compositional matrix adjust. Identities = 40/121 (33%), Positives = 60/121 (49%), Gaps = 5/121 (4%) Query: 103 AVSGFPDVKPGKWYSGTVAKAVELGIVSRSSGKFEPDRGITREEAAVMVAKAARLSTYGS 162 A + F DV G++Y+ V +A LGI+S G F P+ ITREEA V + + + Sbjct: 419 AANPFKDVPAGQYYTDAVTRAASLGIMSGYGGSFRPNDPITREEAVVTLVRTVKYFEVKD 478 Query: 163 AGR--VKFGDTSTLPQDSRQAIQAVNEHEIMTGDGGSFHPKQVLVREQAAAILVRVQKLI 220 G+ F D + + A+ + ++ GDG FHPK L R A I V + +L+ Sbjct: 479 TGKGAPAFADKKQISAWASAAVDQAWKLGLIEGDGKQFHPKAALTR---AEIAVMINRLM 535 Query: 221 P 221 P Sbjct: 536 P 536 >B1D969.1 B1D969_9BACL Cellulosome anchoring protein cohesin region Length = 611 Score = 65.1 bits (157), Expect = 5e-09, Method: Compositional matrix adjust. Identities = 53/184 (28%), Positives = 82/184 (44%), Gaps = 13/184 (7%) Query: 42 TFTDAKQVAVWARSDMAAALSNGYIQGVSASGNVLAPKQKXXXXXXXXXXXXXX-XXXPE 100 TF D WA++D+ S IQGVSA + AP + P+ Sbjct: 423 TFADVTNH--WAKADINDLASRMVIQGVSA--DRFAPDRSVTRAEFTAILLRALGIHAPK 478 Query: 101 DKAVSGFPDVKPGKWYSGTVAKAVELGIVS-RSSGKFEPDRGITREEAAVMVAKAARL-- 157 D F DV WY V AV G+++ S G F P+ I+R+EA VM+ +++ + Sbjct: 479 DDTKVSFRDVGATAWYHDAVVNAVSYGLITGYSDGSFHPNASISRQEAIVMINRSSAITG 538 Query: 158 ----STYGSAGRVK-FGDTSTLPQDSRQAIQAVNEHEIMTGDGGSFHPKQVLVREQAAAI 212 S +AG + F D + + A+ + + I+ G G PKQ + R ++A I Sbjct: 539 LVQASASETAGLISPFNDKKNIDSWALAAVASGAKQGIVKGTNGKLLPKQPITRAESAVI 598 Query: 213 LVRV 216 + RV Sbjct: 599 MKRV 602 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.315 0.130 0.369 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,168,804,875 Number of extensions: 40490230 Number of successful extensions: 77328 Number of sequences better than 1.0e-05: 135 Number of HSP's gapped: 77789 Number of HSP's successfully gapped: 137 Length of query: 225 Length of database: 3,060,696,203 Length adjustment: 131 Effective length of query: 94 Effective length of database: 1,826,543,238 Effective search space: 171695064372 Effective search space used: 171695064372 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 129 (54.3 bits)