BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0548.0031.p (362 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1D5L1.1 B1D5L1_9BACL Oxidoreductase domain protein 536 e-150 B3KFY9.1 B3KFY9_9BACI Oxidoreductase domain protein 536 e-150 Q5WCU9.1 Q5WCU9_BACSK Dehydrogenase 533 e-150 C4C723.1 C4C723_9FUSO Predicted dehydrogenase 523 e-147 B1ZYN5.1 B1ZYN5_OPITP Oxidoreductase domain protein 509 e-142 >B1D5L1.1 B1D5L1_9BACL Oxidoreductase domain protein Length = 368 Score = 536 bits (1380), Expect = e-150, Method: Compositional matrix adjust. Identities = 250/354 (70%), Positives = 293/354 (82%) Query: 1 MSRKDGMNYAPKHEAKPVVGPDEFVIAAIALDHGHIYGMCNGLVEAGAQLKWVYDPDPEK 60 MS+ +GMNYAP+ + +PVV EF+ AA++LDHGHIYGMCNGL EAG LKWV+DPDP K Sbjct: 1 MSKANGMNYAPQGKPRPVVSQGEFIFAAMSLDHGHIYGMCNGLREAGGTLKWVFDPDPAK 60 Query: 61 VKAFIAAYPGVRVARTAEEVLEDPEVRLVAAAAVPSERGPLGLRVMAHGKDYFTDKAPFT 120 V+ F AYP V+VA + E++L+DPEV+LVAAAAV +ERGPLG++VM HGK YFTDKAPFT Sbjct: 61 VEVFCRAYPEVQVALSQEQILQDPEVQLVAAAAVTNERGPLGVKVMKHGKHYFTDKAPFT 120 Query: 121 AMEQLEAARLQAEKTKRKYMCYFSERLHVESAVYAGQLIRQGAIGRVLQLIGMGPHRLNA 180 ++QL A+ A +T +KY Y+SERLHVESA +AGQLI+ GAIGRVLQ+IG+GPHRLNA Sbjct: 121 TLDQLADAKKTASETGKKYSVYYSERLHVESAEFAGQLIKDGAIGRVLQVIGLGPHRLNA 180 Query: 181 PSRPEWFFQREKYGGILCDIGSHQIEQFLYYGNCRDAKVLHSKVANYNNAAYPELEDFGD 240 RPEWFFQ+EKYGGILCDIGSHQIEQFLYY +DAKV SKVANYNN YPELED+GD Sbjct: 181 HMRPEWFFQKEKYGGILCDIGSHQIEQFLYYAGVKDAKVQGSKVANYNNKQYPELEDYGD 240 Query: 241 ATLVGDNGTTQYFRVDWFTPAGLGTWGDGRTIIMGTEGYMELRKYSDIARSNTPDHVFWV 300 ATL+GDNG T YFRVDWFTP GL TWGDGRT+I+GT GY+ELRKY DIARSNT DH+F V Sbjct: 241 ATLIGDNGATNYFRVDWFTPDGLSTWGDGRTMILGTNGYIELRKYVDIARSNTGDHLFLV 300 Query: 301 DGEGEHYEQVAGKVGFPFFGELILDCLERTELAMTQAHVFKAAELCLTAQSQAL 354 +GEGEH +V +VGFP+FG LILD L T+LAM Q H FKAAELCL AQ QA+ Sbjct: 301 NGEGEHKIEVKDQVGFPYFGALILDILNGTDLAMPQEHTFKAAELCLEAQRQAV 354 >B3KFY9.1 B3KFY9_9BACI Oxidoreductase domain protein Length = 357 Score = 536 bits (1380), Expect = e-150, Method: Compositional matrix adjust. Identities = 249/356 (69%), Positives = 294/356 (82%) Query: 1 MSRKDGMNYAPKHEAKPVVGPDEFVIAAIALDHGHIYGMCNGLVEAGAQLKWVYDPDPEK 60 M R +GM YAP +A+PV P EF+ AA++L+HGHIYGMCNGL+EAGA LKWVYDPDP K Sbjct: 1 MDRSNGMLYAPVGKAQPVCKPGEFIFAAMSLEHGHIYGMCNGLIEAGATLKWVYDPDPAK 60 Query: 61 VKAFIAAYPGVRVARTAEEVLEDPEVRLVAAAAVPSERGPLGLRVMAHGKDYFTDKAPFT 120 V AF AYP VA + +VLED EV LVAAAA+PSER LGL+VM+HGKDYFTDK PFT Sbjct: 61 VAAFCKAYPEAAVASSEAQVLEDAEVHLVAAAAIPSERCELGLKVMSHGKDYFTDKTPFT 120 Query: 121 AMEQLEAARLQAEKTKRKYMCYFSERLHVESAVYAGQLIRQGAIGRVLQLIGMGPHRLNA 180 +EQ+ AAR +A +T +KYM Y+SERLHVESAV+AG+LI+ GAIGRV+Q++G GPHRLNA Sbjct: 121 TLEQVNAARAKAAETGKKYMVYYSERLHVESAVHAGKLIQDGAIGRVVQVMGTGPHRLNA 180 Query: 181 PSRPEWFFQREKYGGILCDIGSHQIEQFLYYGNCRDAKVLHSKVANYNNAAYPELEDFGD 240 SRP+WFF+ ++YGGILCDIGSHQIEQFL+Y CRDAKVL SKV NY N YPELEDFGD Sbjct: 181 SSRPDWFFRHQQYGGILCDIGSHQIEQFLFYAGCRDAKVLSSKVGNYANPEYPELEDFGD 240 Query: 241 ATLVGDNGTTQYFRVDWFTPAGLGTWGDGRTIIMGTEGYMELRKYSDIARSNTPDHVFWV 300 ATLVGDNG T YFRVDW TP GLGTWGDGRT I+GTEGY+ELRKY DIAR DH++ V Sbjct: 241 ATLVGDNGATNYFRVDWLTPGGLGTWGDGRTFILGTEGYIELRKYIDIARHPQGDHLYLV 300 Query: 301 DGEGEHYEQVAGKVGFPFFGELILDCLERTELAMTQAHVFKAAELCLTAQSQALNL 356 +GEGE + ++ GKVG+PFFGELILDCLERTE AM+Q H FKA ELC+ AQ++A+ + Sbjct: 301 NGEGEFHMELQGKVGYPFFGELILDCLERTEHAMSQEHAFKAGELCVIAQNEAVRI 356 >Q5WCU9.1 Q5WCU9_BACSK Dehydrogenase Length = 363 Score = 533 bits (1374), Expect = e-150, Method: Compositional matrix adjust. Identities = 248/358 (69%), Positives = 291/358 (81%) Query: 1 MSRKDGMNYAPKHEAKPVVGPDEFVIAAIALDHGHIYGMCNGLVEAGAQLKWVYDPDPEK 60 M++++GMNYAPK + VV DEF +AI LDHGHIYGMCNGL+EAGA L VYDPDP+K Sbjct: 1 MNKQNGMNYAPKGKPNKVVNKDEFRFSAIGLDHGHIYGMCNGLLEAGATLVSVYDPDPKK 60 Query: 61 VKAFIAAYPGVRVARTAEEVLEDPEVRLVAAAAVPSERGPLGLRVMAHGKDYFTDKAPFT 120 V AF+ YP V+VA + ++L D + LVAAAA+PSER LG++VM GKDYFTDK PFT Sbjct: 61 VAAFVDTYPNVQVAGSVADILSDETIALVAAAAIPSERAALGIQVMESGKDYFTDKTPFT 120 Query: 121 AMEQLEAARLQAEKTKRKYMCYFSERLHVESAVYAGQLIRQGAIGRVLQLIGMGPHRLNA 180 ++EQLE A+ E+TK+KYM Y+SERLHVESAV+AG LI+QGAIGRVLQ+ G GPHRLNA Sbjct: 121 SLEQLEQAKATVERTKQKYMVYYSERLHVESAVFAGDLIQQGAIGRVLQVTGFGPHRLNA 180 Query: 181 PSRPEWFFQREKYGGILCDIGSHQIEQFLYYGNCRDAKVLHSKVANYNNAAYPELEDFGD 240 PSRP+WFFQ+EKYGGILCDIGSHQIEQFLY+ RDA VLHSKVANY N YPELED+GD Sbjct: 181 PSRPKWFFQKEKYGGILCDIGSHQIEQFLYFSGARDATVLHSKVANYANPDYPELEDYGD 240 Query: 241 ATLVGDNGTTQYFRVDWFTPAGLGTWGDGRTIIMGTEGYMELRKYSDIARSNTPDHVFWV 300 ATLVGDNG T +F+VDW TP GL TWGDGRT I GTEG +ELRKY D+AR N DH++ V Sbjct: 241 ATLVGDNGATHFFKVDWHTPDGLSTWGDGRTFITGTEGTIELRKYVDVARDNGGDHLYLV 300 Query: 301 DGEGEHYEQVAGKVGFPFFGELILDCLERTELAMTQAHVFKAAELCLTAQSQALNLTP 358 + +GE + ++GKVGFPFFGELILDCL RTE AMTQAH FKAAELCL AQ+QA + P Sbjct: 301 NKDGEQHFALSGKVGFPFFGELILDCLNRTEHAMTQAHAFKAAELCLRAQAQAERMMP 358 >C4C723.1 C4C723_9FUSO Predicted dehydrogenase Length = 357 Score = 523 bits (1348), Expect = e-147, Method: Compositional matrix adjust. Identities = 241/356 (67%), Positives = 285/356 (80%) Query: 1 MSRKDGMNYAPKHEAKPVVGPDEFVIAAIALDHGHIYGMCNGLVEAGAQLKWVYDPDPEK 60 M + DGMNYAPK + KPV EFV AAIALDHGHIYGMCNGL EAGA LK+VYDPDP K Sbjct: 1 MQKTDGMNYAPKGKPKPVCKEGEFVFAAIALDHGHIYGMCNGLTEAGATLKYVYDPDPAK 60 Query: 61 VKAFIAAYPGVRVARTAEEVLEDPEVRLVAAAAVPSERGPLGLRVMAHGKDYFTDKAPFT 120 V+ F+ YP + A + EE+ DPEV+L+A AAVPSER LGLR M HGKDYFTDK+PFT Sbjct: 61 VENFLKVYPNAKAAASEEEIFNDPEVKLIAGAAVPSERCALGLRAMDHGKDYFTDKSPFT 120 Query: 121 AMEQLEAARLQAEKTKRKYMCYFSERLHVESAVYAGQLIRQGAIGRVLQLIGMGPHRLNA 180 MEQLEAA+ +A +T KY Y+SERLHVE AV+AG+LI QGAIG+VLQ+IG+GPHRLN Sbjct: 121 TMEQLEAAKKKAAETGLKYYVYYSERLHVEGAVFAGELINQGAIGKVLQVIGLGPHRLNK 180 Query: 181 PSRPEWFFQREKYGGILCDIGSHQIEQFLYYGNCRDAKVLHSKVANYNNAAYPELEDFGD 240 RPEWFFQ++KYGGILCDIGSHQIEQ+LY+ +DA V+ S+VANY N PELEDFG Sbjct: 181 KDRPEWFFQKDKYGGILCDIGSHQIEQYLYFSGAKDATVVSSEVANYGNPDTPELEDFGH 240 Query: 241 ATLVGDNGTTQYFRVDWFTPAGLGTWGDGRTIIMGTEGYMELRKYSDIARSNTPDHVFWV 300 A L+GDNGTT YFR+DWFTP GLGTWGDGRT+I+GTEGY+E+RKY DIAR N D+V+ V Sbjct: 241 ANLLGDNGTTNYFRIDWFTPDGLGTWGDGRTMILGTEGYIEIRKYIDIARDNKGDNVYLV 300 Query: 301 DGEGEHYEQVAGKVGFPFFGELILDCLERTELAMTQAHVFKAAELCLTAQSQALNL 356 + +GE V G++GFPFFGE ILDC+ RTE +MTQ H FKAAELCL AQ +A+ + Sbjct: 301 NKDGEKRYSVTGEIGFPFFGEFILDCINRTENSMTQEHAFKAAELCLIAQEKAVKI 356 >B1ZYN5.1 B1ZYN5_OPITP Oxidoreductase domain protein Length = 391 Score = 509 bits (1311), Expect = e-142, Method: Compositional matrix adjust. Identities = 236/353 (66%), Positives = 284/353 (80%) Query: 5 DGMNYAPKHEAKPVVGPDEFVIAAIALDHGHIYGMCNGLVEAGAQLKWVYDPDPEKVKAF 64 DGMNYAP+ + +PVV P EF+ AA LDHGHI+G CNGL EAGAQLKW+YDPD +KV A Sbjct: 39 DGMNYAPQGKPRPVVKPGEFIFAAAHLDHGHIHGQCNGLTEAGAQLKWIYDPDAKKVAAL 98 Query: 65 IAAYPGVRVARTAEEVLEDPEVRLVAAAAVPSERGPLGLRVMAHGKDYFTDKAPFTAMEQ 124 +P +VAR+ +E+L DP V+LVA+AAV SERGPLG RVM GKDYFTDKAPFT ++Q Sbjct: 99 HERFPDAKVARSLDEILADPAVQLVASAAVTSERGPLGCRVMEAGKDYFTDKAPFTTLDQ 158 Query: 125 LEAARLQAEKTKRKYMCYFSERLHVESAVYAGQLIRQGAIGRVLQLIGMGPHRLNAPSRP 184 LE A+ +T RKYM Y+SER+H ESA++A L++QGAIGRVLQ+IG+GPHRLNAPSRP Sbjct: 159 LEQAKAVVARTGRKYMVYYSERIHNESAMFATDLVQQGAIGRVLQVIGLGPHRLNAPSRP 218 Query: 185 EWFFQREKYGGILCDIGSHQIEQFLYYGNCRDAKVLHSKVANYNNAAYPELEDFGDATLV 244 WFF+REK+GGILCDIGSHQ EQFL Y +DA V+H+ +ANY + YP EDFG+A+L+ Sbjct: 219 AWFFEREKFGGILCDIGSHQFEQFLTYTGAKDANVVHAAIANYAHKEYPTFEDFGEASLI 278 Query: 245 GDNGTTQYFRVDWFTPAGLGTWGDGRTIIMGTEGYMELRKYSDIARSNTPDHVFWVDGEG 304 GDN TT + RVDWFTPAGLG WGDGRTII+GT+GY+ELRKY ++ R T DHV+ VD +G Sbjct: 279 GDNATTNFIRVDWFTPAGLGVWGDGRTIILGTKGYIELRKYIEVGREKTGDHVYVVDEKG 338 Query: 305 EHYEQVAGKVGFPFFGELILDCLERTELAMTQAHVFKAAELCLTAQSQALNLT 357 E + VAG+VGF FFGELILDCL RTE AMTQAH FKAAELCL AQ++A LT Sbjct: 339 EQHLHVAGQVGFRFFGELILDCLNRTEKAMTQAHAFKAAELCLRAQAEARWLT 391 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.138 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,299,663,692 Number of extensions: 99652306 Number of successful extensions: 206843 Number of sequences better than 1.0e-05: 89 Number of HSP's gapped: 207063 Number of HSP's successfully gapped: 89 Length of query: 362 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 225 Effective length of database: 1,770,017,148 Effective search space: 398253858300 Effective search space used: 398253858300 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)