BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0548.0030.p (194 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A4XH41.1 A4XH41_CALS8 Xylose isomerase domain protein TIM barrel 187 3e-46 B9MMV4.1 B9MMV4_ANATD Xylose isomerase domain protein TIM barrel 181 3e-44 A6PUU2.1 A6PUU2_9BACT Xylose isomerase domain protein TIM barrel 163 7e-39 B1DEK8.1 B1DEK8_9BACL Xylose isomerase domain protein TIM barrel 155 1e-36 A7NGE0.1 A7NGE0_ROSCS Xylose isomerase domain protein TIM barrel 154 4e-36 >A4XH41.1 A4XH41_CALS8 Xylose isomerase domain protein TIM barrel Length = 255 Score = 187 bits (476), Expect = 3e-46, Method: Compositional matrix adjust. Identities = 86/191 (45%), Positives = 132/191 (69%), Gaps = 3/191 (1%) Query: 1 VICATHIPWDRLVNDLDALAAEHKLWNCKYVGLGALPGEYQTSQEGYRTFAKKVSETALV 60 +CATH P++RL N+++++ EHK+ C ++ + + P +++ S EG FAK+ +E Sbjct: 61 TVCATHTPFERLKNEIESVIEEHKILGCYHIVIPSAPNKFR-SFEGALEFAKECNEIGKK 119 Query: 61 LKQKHGLQFVYHNHDFEFERLDGVTGLEVLLQEADPAAFDFELDLYWVQAGGGSPVDWIH 120 LK+K+ L YHNH FE +R D T LE+L++ +DP FE+D YWVQ G SP WI Sbjct: 120 LKEKNIL-LSYHNHSFELKRYDSKTWLEILIENSDPEVLMFEIDTYWVQFAGASPEKWIR 178 Query: 121 RVKDRMRVVHLKDMAVVAR-KAEFAEIGQGNMNYPAIIEACRETGVEWFVVEQDVCRRDP 179 ++K R+ +VHLKDM ++ K E+G GN+++ IIEAC E+GV+W+++EQD+C++ P Sbjct: 179 KLKGRIPLVHLKDMGMLEDFKQGMFEVGFGNLDWDGIIEACNESGVQWYIIEQDICQKSP 238 Query: 180 FESLKISLDYL 190 FESLK+SLD+L Sbjct: 239 FESLKLSLDFL 249 >B9MMV4.1 B9MMV4_ANATD Xylose isomerase domain protein TIM barrel Length = 255 Score = 181 bits (458), Expect = 3e-44, Method: Compositional matrix adjust. Identities = 82/191 (42%), Positives = 130/191 (68%), Gaps = 5/191 (2%) Query: 2 ICATHIPWDRLVNDLDALAAEHKLWNCKYVGLGALPGEYQTSQEGYRTFAKKVSETALVL 61 ICATHIP++RL +++++ +EHK++ C ++ + + P EY+ SQEG FA + +E L Sbjct: 62 ICATHIPFERLKEEIESVISEHKIYECLHIAIPSAPSEYR-SQEGAIAFAAECNEIGKKL 120 Query: 62 KQKHGLQFVYHNHDFEFERLDGVTGLEVLLQEADPAAFDFELDLYWVQAGGGSPVDWIHR 121 K + G+ YHNH FEF++ DG T E+ ++ ++P E+D YW+Q G +P WI Sbjct: 121 KDE-GITLSYHNHSFEFKKYDGKTWFELFIESSNPEYLMIEIDTYWIQFAGANPEKWIRS 179 Query: 122 VKDRMRVVHLKDMAVVA--RKAEFAEIGQGNMNYPAIIEACRETGVEWFVVEQDVCRRDP 179 ++ R+ ++HLKDM ++ R+ F E+G GN+++ II +C E GVEW+++EQDVC+R P Sbjct: 180 LEGRIPLIHLKDMGMIEDFRQGMF-EVGFGNLDWDGIIASCNEAGVEWYIIEQDVCQRLP 238 Query: 180 FESLKISLDYL 190 FESLK+S D+L Sbjct: 239 FESLKMSFDFL 249 >A6PUU2.1 A6PUU2_9BACT Xylose isomerase domain protein TIM barrel Length = 261 Score = 163 bits (413), Expect = 7e-39, Method: Compositional matrix adjust. Identities = 87/196 (44%), Positives = 114/196 (58%), Gaps = 6/196 (3%) Query: 3 CATHIPWDRLVNDLDALAAEHKLWNCKYVGLGALPGEYQTSQEGYRTFAKKVSETALVLK 62 CATH + D+ L + C + LGA P EY+T+ EG K +E L Sbjct: 64 CATHENLQTISGDVAPLIDRLQTLGCDFTALGAPPEEYRTTVEGVARLVKIFNEQGAKLM 123 Query: 63 QKHGLQFVYHNHDFEFERLDGV--TGLEVLLQEADPAAFDFELDLYWVQAGGGSPVDWIH 120 +K G++ YHNH FEF RL G T LE + D E+D++WV GGGSPV WI Sbjct: 124 EK-GIRLGYHNHHFEFIRLPGTKKTMLETFYDDTDSRCVSAEIDVHWVTRGGGSPVRWIR 182 Query: 121 RVKDRMRVVHLKDMAVVA-RKAEFAEIGQGNMNYPAIIEACRETGVEWFVVEQDVC--RR 177 +V RM V+H KD AV+ + F EIG+GN+++PAIIEACRETGV W+ +EQD R Sbjct: 183 KVAGRMPVIHFKDFAVIGNNEPAFCEIGEGNLDWPAIIEACRETGVRWYSIEQDALFQNR 242 Query: 178 DPFESLKISLDYLHTL 193 D F+S+KIS D L + Sbjct: 243 DIFDSIKISFDNLKAM 258 >B1DEK8.1 B1DEK8_9BACL Xylose isomerase domain protein TIM barrel Length = 254 Score = 155 bits (393), Expect = 1e-36, Method: Compositional matrix adjust. Identities = 79/195 (40%), Positives = 117/195 (60%), Gaps = 5/195 (2%) Query: 2 ICATHIPWDRLVNDLDALAAEHKLWNCKYVGLGALPGEYQTSQEGYRTFAKKVSETALVL 61 I TH DR++N+ + + EH + C Y+G+G +P +Y+ + Y FA+ E A+ Sbjct: 59 IALTHTNADRILNETEKVIEEHDILGCNYIGIGGMPDKYREP-DWYGYFAEDFRE-AVGK 116 Query: 62 KQKHGLQFVYHNHDFEFERL---DGVTGLEVLLQEADPAAFDFELDLYWVQAGGGSPVDW 118 K G F+YHNH FEFE+ +G +E L+++ P LD YWVQA G +W Sbjct: 117 IAKAGKLFMYHNHHFEFEKFKKWNGKRLMERLIEDFSPEEMGITLDTYWVQAAGADVCEW 176 Query: 119 IHRVKDRMRVVHLKDMAVVARKAEFAEIGQGNMNYPAIIEACRETGVEWFVVEQDVCRRD 178 I ++KDR+ VHLKDMAV + A +G+GNMN+PAI++A +T ++ +VEQDVC+ Sbjct: 177 IGKLKDRLHCVHLKDMAVRGTEQLMAPVGEGNMNFPAILKALEQTNAKYLLVEQDVCQTS 236 Query: 179 PFESLKISLDYLHTL 193 PFE L+ S + L L Sbjct: 237 PFECLQTSYNNLAAL 251 >A7NGE0.1 A7NGE0_ROSCS Xylose isomerase domain protein TIM barrel Length = 252 Score = 154 bits (389), Expect = 4e-36, Method: Compositional matrix adjust. Identities = 77/194 (39%), Positives = 112/194 (57%), Gaps = 3/194 (1%) Query: 1 VICATHIPWDRLVNDLDALAAEHKLWNCKYVGLGALPGEYQTSQEGYRTFAKKVSETALV 60 ++ H+P L + L+A+ A+ + Y+ +P E + S + YR A+ ++ Sbjct: 59 MVSGAHVPIQMLESQLNAIIADMQTLGAPYLICPWMPPERRRSADDYRALARILNGIGAA 118 Query: 61 LKQKHGLQFVYHNHDFEFERLDGVTGLEVLLQEADPAAFDFELDLYWVQAGGGSPVDWIH 120 + GL F YH+HDFE L TGL +LL E DP A FE+D+YW G PV I+ Sbjct: 119 VADA-GLTFCYHHHDFELHLLGDTTGLHLLLHECDPRAVAFEIDVYWSAYAGVDPVQLIN 177 Query: 121 RVKDRMRVVHLKDMAVVARKAEFAEIGQGNMNYPAIIEACRETGVEWFVVEQDVCRRDPF 180 R ++HLKDMA +R FAE+G G ++ PAI+ A R G +WFVVEQDVC+R P Sbjct: 178 EFAGRAPLIHLKDMAPGSRT--FAEVGHGTLDIPAILSAARTAGAQWFVVEQDVCQRPPL 235 Query: 181 ESLKISLDYLHTLL 194 ES+++S +YL L+ Sbjct: 236 ESVRMSYEYLKGLM 249 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.323 0.139 0.438 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,209,191,874 Number of extensions: 47131503 Number of successful extensions: 101024 Number of sequences better than 1.0e-05: 207 Number of HSP's gapped: 100777 Number of HSP's successfully gapped: 207 Length of query: 194 Length of database: 3,060,696,203 Length adjustment: 128 Effective length of query: 66 Effective length of database: 1,854,806,283 Effective search space: 122417214678 Effective search space used: 122417214678 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 127 (53.5 bits)