BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0548.0028.p (391 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KFY8.1 B3KFY8_9BACI Oxidoreductase domain protein 559 e-157 B1D5L2.1 B1D5L2_9BACL Oxidoreductase domain protein 543 e-152 Q5WCV0.1 Q5WCV0_BACSK Dehydrogenase 487 e-136 B6F7Z5.1 B6F7Z5_9ENTR Putative uncharacterized protein 463 e-128 C4C725.1 C4C725_9FUSO Predicted dehydrogenase 426 e-117 >B3KFY8.1 B3KFY8_9BACI Oxidoreductase domain protein Length = 389 Score = 559 bits (1441), Expect = e-157, Method: Compositional matrix adjust. Identities = 262/374 (70%), Positives = 306/374 (81%), Gaps = 3/374 (0%) Query: 5 MAKVRYGIIGIGNMGRAHALSLL-DDVKGAELAAVCDISPERLEWASEQLPEKVKHFSTA 63 M +VR G+IG+G MG +HA SL + GA L+A+CD++P + E A LP VK +S Sbjct: 1 MEQVRVGVIGLG-MGFSHAKSLAAGRIAGARLSAICDLNPAKQEEARATLPSDVKIWSDT 59 Query: 64 AEMFQSGTIDAVLISTPHYDHPPLAIEALGYGLHVLIEKPAGVHTKAVQEMNEAAAKSDR 123 M SG IDAV+I+TPHY HP +AI L GLHVLIEKPAGV+TK V+EMN AA S + Sbjct: 60 DAMISSGEIDAVIIATPHYAHPQVAIACLNQGLHVLIEKPAGVYTKQVREMNRAAEASGK 119 Query: 124 KFGIMYNQRTNPLYQKLRELIQAGELGEIRRTNWIITNWYRSQNYYDSGGWRATWAGEGG 183 F IMYNQR NPLY+KLRELI AGELGE+RR NWI+TNWYRSQ+YYDSGGWRATWAGEGG Sbjct: 120 VFSIMYNQRCNPLYKKLRELIAAGELGEVRRMNWIVTNWYRSQSYYDSGGWRATWAGEGG 179 Query: 184 GVLLNQDPHQLDLWQWTTGMMPKRIRAFCYFGKYRNIEVEDDVTAYVEYPNGATGLFITT 243 GVL+NQ PHQLDLWQWTTGMMPKRIRAFC FGKYRNIEVEDDVTAY EY NGAT +FITT Sbjct: 180 GVLINQSPHQLDLWQWTTGMMPKRIRAFCSFGKYRNIEVEDDVTAYAEYENGATAVFITT 239 Query: 244 TGEAPGTNRFEITGDNGKILVEDGALTFWRLRTPEPVFNAEYKGGFGAPECWKCEIPVTK 303 TGEAPGTNR+E+TGD GKI++EDG LTFWRLR PEP FN +Y GGFG PECWKCE+P++ Sbjct: 240 TGEAPGTNRYEVTGDRGKIVIEDGKLTFWRLRVPEPEFNQQYTGGFGEPECWKCEVPIS- 298 Query: 304 GDGEQHRGILRNFTNAILYDEPLLAPGEEGIHGLTLSNAMYLSAWTDNWVDLPIDSDLFH 363 GD QH GI++NF +AIL+ PL+APGEEGIHGLTLSNAM+LS WTDNWVDLP+D DL++ Sbjct: 299 GDNPQHNGIIQNFADAILHGTPLIAPGEEGIHGLTLSNAMHLSTWTDNWVDLPLDEDLYY 358 Query: 364 EKLMEKVQNSTFQK 377 E L E++ NS+ +K Sbjct: 359 EMLQERIANSSTRK 372 >B1D5L2.1 B1D5L2_9BACL Oxidoreductase domain protein Length = 385 Score = 543 bits (1398), Expect = e-152, Method: Compositional matrix adjust. Identities = 260/385 (67%), Positives = 308/385 (80%), Gaps = 2/385 (0%) Query: 8 VRYGIIGIGNMGRAHALSLLDD-VKGAELAAVCDISPERLEWASEQLPEKVKHFSTAAEM 66 VR GI+G+GNMG HA L+ VKGAEL AV D+ PERL + E ++ F + EM Sbjct: 2 VRIGIVGLGNMGTGHARYLITGGVKGAELTAVSDVRPERLAEVKAEWGEHIQGFLSPQEM 61 Query: 67 FQSGTIDAVLISTPHYDHPPLAIEALGYGLHVLIEKPAGVHTKAVQEMNEAAAKSDRKFG 126 F+SGT+D V+I TPHYDHP AI AL GLHVLIEKPAGV+T+ V+EMN AAAKSD+ FG Sbjct: 62 FESGTVDGVIICTPHYDHPEQAIAALNAGLHVLIEKPAGVYTRTVREMNNAAAKSDKVFG 121 Query: 127 IMYNQRTNPLYQKLRELIQAGELGEIRRTNWIITNWYRSQNYYDSGGWRATWAGEGGGVL 186 IMYNQRTNPLY KLRELI +GELGEIRRTNWIIT+WYRSQ+YY+SGGWRATWAGEGGGVL Sbjct: 122 IMYNQRTNPLYAKLRELIASGELGEIRRTNWIITDWYRSQSYYNSGGWRATWAGEGGGVL 181 Query: 187 LNQDPHQLDLWQWTTGMMPKRIRAFCYFGKYRNIEVEDDVTAYVEYPNGATGLFITTTGE 246 +NQDPHQLDLWQWTTG+MPKR+RAFC FGK+R+IEVE+DVTAYVEY NGATG+F+T+T E Sbjct: 182 INQDPHQLDLWQWTTGLMPKRVRAFCQFGKHRDIEVENDVTAYVEYENGATGVFVTSTFE 241 Query: 247 APGTNRFEITGDNGKILVEDGALTFWRLRTPEPVFNAEYKGGFGAPECWKCEIPVTKGDG 306 +PGTNRFEI+GD GKI++ED LTF RLR E FN + GGFG PECWK EIP+T G G Sbjct: 242 SPGTNRFEISGDRGKIVIEDNKLTFHRLRVLESEFNETFTGGFGQPECWKFEIPIT-GSG 300 Query: 307 EQHRGILRNFTNAILYDEPLLAPGEEGIHGLTLSNAMYLSAWTDNWVDLPIDSDLFHEKL 366 H+ I +N+ +AI PL+APGEEGI GL +SNAM LS WTDNWVDLPID DLF++KL Sbjct: 301 GSHQEITQNWADAINKGTPLVAPGEEGIKGLMISNAMLLSTWTDNWVDLPIDEDLFYDKL 360 Query: 367 MEKVQNSTFQKDTTVQKTLNVTGTH 391 E++ S +K+ TL+V+GTH Sbjct: 361 QEQIAKSDSKKNANEYHTLDVSGTH 385 >Q5WCV0.1 Q5WCV0_BACSK Dehydrogenase Length = 386 Score = 487 bits (1253), Expect = e-136, Method: Compositional matrix adjust. Identities = 234/388 (60%), Positives = 292/388 (75%), Gaps = 3/388 (0%) Query: 5 MAKVRYGIIGIGNMGRAHALSLLD-DVKGAELAAVCDISPERLEWASEQLPEKVKHFSTA 63 M +VR G+IG+GNMG AHA L++ +KGA L A+ + ER + + V F+ Sbjct: 1 MDQVRIGVIGVGNMGSAHAAYLVEGSIKGACLGALLEEDGERASAVQQHFGDNVPVFTNE 60 Query: 64 AEMFQSGTIDAVLISTPHYDHPPLAIEALGYGLHVLIEKPAGVHTKAVQEMNEAAAKSDR 123 ++ F + +DAVLI+TPHY H LA +A GLHVL EKPAGV+T+ V+EMNEAA KS R Sbjct: 61 SDFFHANVVDAVLIATPHYSHSRLAEQAFAAGLHVLCEKPAGVYTRQVREMNEAAQKSGR 120 Query: 124 KFGIMYNQRTNPLYQKLRELIQAGELGEIRRTNWIITNWYRSQNYYDSGGWRATWAGEGG 183 + +MYNQRT P+Y KL++LIQ+GELG IRR NWIIT+WYRSQ+YYDS WRATW GEGG Sbjct: 121 VYSLMYNQRTKPIYLKLKDLIQSGELGTIRRMNWIITDWYRSQSYYDSSSWRATWPGEGG 180 Query: 184 GVLLNQDPHQLDLWQWTTGMMPKRIRAFCYFGKYRNIEVEDDVTAYVEYPNGATGLFITT 243 GVL+NQ PHQLDLW W GM P RIRAFC FGKYR+IEVED+VTAY+EY +GATG+FITT Sbjct: 181 GVLINQCPHQLDLWGWLIGMEPNRIRAFCSFGKYRDIEVEDEVTAYMEYESGATGVFITT 240 Query: 244 TGEAPGTNRFEITGDNGKILVEDGALTFWRLRTPEPVFNAEYKGGFGAPECWKCEIPVTK 303 T EAPGTNR E+TG GK ++E L+FW+LRTPEPVFNA YKGGFGAPE WK +IPV Sbjct: 241 TAEAPGTNRLEVTGTKGKAVIEGETLSFWQLRTPEPVFNASYKGGFGAPEHWKIDIPVA- 299 Query: 304 GDGEQHRGILRNFTNAILYDEPLLAPGEEGIHGLTLSNAMYLSAWTDNWVDLPIDSDLFH 363 G H+GI +NF +AIL+ PL+APGEEGI GLTLSNAM+LS W DNWV+LP++ L+ Sbjct: 300 GAETGHQGITQNFVDAILHGVPLIAPGEEGIQGLTLSNAMHLSTWIDNWVELPLNEALYE 359 Query: 364 EKLMEKVQNSTFQKDTTVQKTLNVTGTH 391 + L E+++NS K T +TL+V ++ Sbjct: 360 QNLNERIRNSP-GKTVTTNQTLDVRKSY 386 >B6F7Z5.1 B6F7Z5_9ENTR Putative uncharacterized protein Length = 383 Score = 463 bits (1191), Expect = e-128, Method: Compositional matrix adjust. Identities = 238/387 (61%), Positives = 287/387 (74%), Gaps = 6/387 (1%) Query: 5 MAKVRYGIIGIGNMGRAHALSLL-DDVKGAELAAVCDISPERLEWASEQLPEKVKHFSTA 63 M KVR+GIIGIGN+G HA LL V A L AVCD +PE+ A QL + FS A Sbjct: 1 MEKVRFGIIGIGNIGTVHARYLLAGTVSDACLTAVCDNAPEKHP-AIRQLTGDIPLFSNA 59 Query: 64 AEMFQSGTIDAVLISTPHYDHPPLAIEALGYGLHVLIEKPAGVHTKAVQEMNEAAAKSDR 123 EM QSG IDAV+++TPHYDHP L++ A+ G+H L EKPAGV+T VQEMN A + D Sbjct: 60 QEMLQSGLIDAVIVATPHYDHPRLSMLAMRSGIHTLCEKPAGVYTAQVQEMNACARECDV 119 Query: 124 KFGIMYNQRTNPLYQKLRELIQAGELGEIRRTNWIITNWYRSQNYYDSGGWRATWAGEGG 183 FGIMYNQR NPLYQK+++LI +GELGEIRR+NW+ITNWYRSQ+YY+SGGWRATW GEGG Sbjct: 120 TFGIMYNQRPNPLYQKVKDLIDSGELGEIRRSNWVITNWYRSQSYYNSGGWRATWKGEGG 179 Query: 184 GVLLNQDPHQLDLWQWTTGMMPKRIRAFCYFGKYRNIEVEDDVTAYVEYPNGATGLFITT 243 GVLLNQDPHQLDLWQW G MP R+RAFC FGK+R+IEVED+VTAY EY NGATG+FITT Sbjct: 180 GVLLNQDPHQLDLWQWLVG-MPVRLRAFCQFGKHRDIEVEDEVTAYAEYANGATGVFITT 238 Query: 244 TGEAPGTNRFEITGDNGKILVEDGALTFWRLRTPEPVFNAEYKGGFGAPECWKCEIPVTK 303 E PGTNR EI GD GK++VE+G L FWRLR E FNA ++ GFG PECW+ IPV Sbjct: 239 VAETPGTNRLEIAGDRGKVVVEEGKLRFWRLRESETAFNARWQNGFGEPECWEVTIPVAP 298 Query: 304 GDGEQHRGILRNFTNAILYDEPLLAPGEEGIHGLTLSNAMYLSAWTDNWVDLPIDSDLFH 363 E H I NF AIL EPL+APG EGI GLTLSNAM+LS WTD+WV+LP++ + Sbjct: 299 EYSEHHV-ITANFCAAILRGEPLIAPGLEGIRGLTLSNAMHLSTWTDDWVELPLNEKQYL 357 Query: 364 EKLMEKVQNSTFQKDTTVQKTLNVTGT 390 L +++ S ++ T++ TL+ +GT Sbjct: 358 RLLQQRIATSADKEATSI--TLDASGT 382 >C4C725.1 C4C725_9FUSO Predicted dehydrogenase Length = 383 Score = 426 bits (1096), Expect = e-117, Method: Compositional matrix adjust. Identities = 201/377 (53%), Positives = 269/377 (71%), Gaps = 9/377 (2%) Query: 8 VRYGIIGIGNMGRAHALSLLDDV-KGAELAAVCDISPERLEWASEQLPEKVKHFSTAAEM 66 V+ G+IG GN+G H + V +L AV DI E+++ A K+K+F+ + Sbjct: 2 VKIGVIGYGNIGSMHVRNFESGVFSNVQLTAVFDIDSEKIKKAENLYGNKLKYFTKEEDF 61 Query: 67 FQSGTIDAVLISTPHYDHPPLAIEALGYGLHVLIEKPAGVHTKAVQEMNEAAAKSDRKFG 126 F S DAVL++TPHY HP LA +AL G HVL+EKP GV+TKAV+ + + AA+SD+ FG Sbjct: 62 FGSHEFDAVLVATPHYAHPVLAEKALDNGYHVLVEKPIGVYTKAVRRLYKKAAESDKIFG 121 Query: 127 IMYNQRTNPLYQKLRELIQAGELGEIRRTNWIITNWYRSQNYYDSGGWRATWAGEGGGVL 186 +++NQRTNPLYQK+++L+ +GELG ++R NWIIT+WYR+Q YY+SG WRATW GEGGGVL Sbjct: 122 VVFNQRTNPLYQKVKDLVSSGELGTLKRINWIITDWYRTQAYYNSGTWRATWGGEGGGVL 181 Query: 187 LNQDPHQLDLWQWTTGMMPKRIRAFCYFGKYRNIEVEDDVTAYVEYPNGATGLFITTTGE 246 +NQ PHQLDL+QW G MPKR+R F YFGK RNIEVED+VTAY EY NGATG+FI++ E Sbjct: 182 INQCPHQLDLFQWIFG-MPKRVRGFAYFGKNRNIEVEDEVTAYFEYENGATGVFISSVSE 240 Query: 247 APGTNRFEITGDNGKILVEDGALTFWRLRTPEPVFNAEYK-------GGFGAPECWKCEI 299 PG+NR EITGD GK+++E+ + F+RLR E +N K G F PE WKCE+ Sbjct: 241 VPGSNRLEITGDKGKVIIENSQIEFYRLRESEIEYNKNLKVQGFDKTGNFIMPEYWKCEV 300 Query: 300 PVTKGDGEQHRGILRNFTNAILYDEPLLAPGEEGIHGLTLSNAMYLSAWTDNWVDLPIDS 359 PV K D QH +++++ + I+ PLLAPG + I+ L +SNA+YLS + DNWV+ PID Sbjct: 301 PVEKSDESQHVLVIKDWIDTIINGTPLLAPGTDAINELMISNAVYLSTFIDNWVEFPIDE 360 Query: 360 DLFHEKLMEKVQNSTFQ 376 DLF EKL E+V+ S ++ Sbjct: 361 DLFLEKLNERVRKSKYK 377 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.136 0.425 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,634,503,267 Number of extensions: 121799198 Number of successful extensions: 285717 Number of sequences better than 1.0e-05: 3730 Number of HSP's gapped: 282740 Number of HSP's successfully gapped: 3730 Length of query: 391 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 253 Effective length of database: 1,760,596,133 Effective search space: 445430821649 Effective search space used: 445430821649 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)