BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0548.0026.p (287 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1D9F4.1 B1D9F4_9BACL Short-chain dehydrogenase/reductase SDR 421 e-116 B3K848.1 B3K848_9BACI Short-chain dehydrogenase/reductase SDR 399 e-109 Q5WKD4.1 Q5WKD4_BACSK Mannonate oxidoreductase 387 e-106 B4AIV3.1 B4AIV3_BACPU D-mannonate oxidoreductase 379 e-103 Q65L03.1 Q65L03_BACLD YjmF 372 e-101 >B1D9F4.1 B1D9F4_9BACL Short-chain dehydrogenase/reductase SDR Length = 282 Score = 421 bits (1081), Expect = e-116, Method: Compositional matrix adjust. Identities = 203/275 (73%), Positives = 234/275 (85%) Query: 13 LAGKVAVITGGAGVLGAVMAHELGRQGAKVAILNRTAEKGESLAAGINAAGGQAVSVACD 72 LAGKVAVITGG+GVL A MA ELGRQG KVAILNRTAEKGE++A I AGG A++VAC+ Sbjct: 8 LAGKVAVITGGSGVLCAEMARELGRQGVKVAILNRTAEKGEAVAREIREAGGVAIAVACN 67 Query: 73 VTEDSSVHRAAAEVLNHWGPCDLLINGAGGNQPSAVTTKEIFTEGDLENPEITSFFELSA 132 V + SV A+A VL G CD+LINGAGGN P +TTKE F DLENP++TSFF+L Sbjct: 68 VVDAESVKEASAVVLEQLGSCDILINGAGGNHPDGITTKETFHPDDLENPDVTSFFDLKK 127 Query: 133 AGFLQVMDLNLIGTLIPTQIFARQMLGRPGASVINISSMSAAAPMTKVPAYSAAKAGVNN 192 GF V DLNL+GTLIPTQ+FA+QM+ +PGA+VIN+SSMSA +PMTKVPAYSAAKA +NN Sbjct: 128 EGFGFVFDLNLLGTLIPTQVFAKQMINKPGATVINMSSMSAPSPMTKVPAYSAAKAAINN 187 Query: 193 FTQWLAVHLAEAGIRVNAIAPGFFLTKQNEKLLLREDGELTERSHKIISQTPMRRFGKPE 252 FTQWLAVH+A+ GIRVNAIAPGFFLT+QN KLL EDG LTERS+KII+ TPMRRFGKPE Sbjct: 188 FTQWLAVHMADVGIRVNAIAPGFFLTEQNRKLLTNEDGSLTERSNKIITHTPMRRFGKPE 247 Query: 253 ELLGTLLWLADESMSGFVTGITVPVDGGFMAYSGV 287 +LLGTL+WLAD+S+SGFVTGITVPVDGGFMAYSGV Sbjct: 248 DLLGTLVWLADDSVSGFVTGITVPVDGGFMAYSGV 282 >B3K848.1 B3K848_9BACI Short-chain dehydrogenase/reductase SDR Length = 311 Score = 399 bits (1024), Expect = e-109, Method: Compositional matrix adjust. Identities = 194/282 (68%), Positives = 221/282 (78%) Query: 6 KLALQPALAGKVAVITGGAGVLGAVMAHELGRQGAKVAILNRTAEKGESLAAGINAAGGQ 65 L + P+L GK VITGG+GVL MA ELGRQ AKVA+LNRTAEKG+ +A I AAGG Sbjct: 30 SLPIHPSLNGKTVVITGGSGVLCRAMALELGRQSAKVAVLNRTAEKGQQVADEITAAGGT 89 Query: 66 AVSVACDVTEDSSVHRAAAEVLNHWGPCDLLINGAGGNQPSAVTTKEIFTEGDLENPEIT 125 A++V CDVT+ SV +A V +GPCD+LINGAGGN+ A TT EIF D+ ++T Sbjct: 90 AIAVPCDVTDVESVRQAEKAVRESFGPCDILINGAGGNRKDANTTNEIFRPEDVGQSDVT 149 Query: 126 SFFELSAAGFLQVMDLNLIGTLIPTQIFARQMLGRPGASVINISSMSAAAPMTKVPAYSA 185 +FF LS GF V DLN IGTLIPTQ+FA MLGRPGA V+NISSMSA +PMTKVPAYSA Sbjct: 150 TFFGLSVPGFRSVFDLNFIGTLIPTQVFAEGMLGRPGAVVLNISSMSAPSPMTKVPAYSA 209 Query: 186 AKAGVNNFTQWLAVHLAEAGIRVNAIAPGFFLTKQNEKLLLREDGELTERSHKIISQTPM 245 AKA +NNFTQWL+VHLAE+GIRVNAIAPGFFLT+QN LL DG LTERSHKIIS TPM Sbjct: 210 AKAAINNFTQWLSVHLAESGIRVNAIAPGFFLTEQNRNLLTHSDGSLTERSHKIISHTPM 269 Query: 246 RRFGKPEELLGTLLWLADESMSGFVTGITVPVDGGFMAYSGV 287 RRFG PE+LLGTLLWL D+ SGFVTG +PVDGGFMAYSGV Sbjct: 270 RRFGVPEDLLGTLLWLVDDQTSGFVTGTVIPVDGGFMAYSGV 311 >Q5WKD4.1 Q5WKD4_BACSK Mannonate oxidoreductase Length = 281 Score = 387 bits (994), Expect = e-106, Method: Compositional matrix adjust. Identities = 189/276 (68%), Positives = 219/276 (79%), Gaps = 1/276 (0%) Query: 12 ALAGKVAVITGGAGVLGAVMAHELGRQGAKVAILNRTAEKGESLAAGINAAGGQAVSVAC 71 +L GKVAV+TGG+G+L MA EL G KVAILNRT EKGE++A I GG+A+++AC Sbjct: 7 SLKGKVAVVTGGSGILCREMAKELAAHGMKVAILNRTKEKGEAVAEEIKEQGGKAIALAC 66 Query: 72 DVTEDSSVHRAAAEVLNHWGPCDLLINGAGGNQPSAVTTKEIFTEGDLENPEITSFFELS 131 DV ++ V +AA+ V +GPCDLLINGAGGN P A+T KE + EGDLEN SFF+LS Sbjct: 67 DVLKEEQVQQAASTVKEEFGPCDLLINGAGGNHPDAITDKETYEEGDLENESAKSFFDLS 126 Query: 132 AAGFLQVMDLNLIGTLIPTQIFARQMLGRPGASVINISSMSAAAPMTKVPAYSAAKAGVN 191 GF V LNL GT+IPTQ FA+QMLGR G SVINISSMSA +PMTKVPAYSAAKAG+N Sbjct: 127 PNGFDSVFRLNLQGTVIPTQAFAKQMLGR-GGSVINISSMSAPSPMTKVPAYSAAKAGIN 185 Query: 192 NFTQWLAVHLAEAGIRVNAIAPGFFLTKQNEKLLLREDGELTERSHKIISQTPMRRFGKP 251 NFTQWLAVH A++GIR NAIAPGFFLT+QN LL DG LTER+ KII+ TP RRFGKP Sbjct: 186 NFTQWLAVHFADSGIRANAIAPGFFLTEQNRALLTNPDGSLTERAEKIIAHTPQRRFGKP 245 Query: 252 EELLGTLLWLADESMSGFVTGITVPVDGGFMAYSGV 287 E+LLGTLLWLADE S FVTG+T+PVDGGFMAYSGV Sbjct: 246 EDLLGTLLWLADEDASRFVTGVTIPVDGGFMAYSGV 281 >B4AIV3.1 B4AIV3_BACPU D-mannonate oxidoreductase Length = 284 Score = 379 bits (973), Expect = e-103, Method: Compositional matrix adjust. Identities = 183/284 (64%), Positives = 212/284 (74%) Query: 4 MAKLALQPALAGKVAVITGGAGVLGAVMAHELGRQGAKVAILNRTAEKGESLAAGINAAG 63 M + LAGKVA ITGG+GVL + MA EL R G VAILNRT EKGE +A IN G Sbjct: 1 MVNIQENKNLAGKVAAITGGSGVLCSAMAKELARHGVSVAILNRTVEKGEKVAEEINLMG 60 Query: 64 GQAVSVACDVTEDSSVHRAAAEVLNHWGPCDLLINGAGGNQPSAVTTKEIFTEGDLENPE 123 G+A+++ CDV SV +A V G C +LINGAGGN SA TT EIF DL NP+ Sbjct: 61 GKAIAIKCDVLNKESVEQAEKIVTEELGMCTILINGAGGNHSSANTTNEIFQTEDLTNPK 120 Query: 124 ITSFFELSAAGFLQVMDLNLIGTLIPTQIFARQMLGRPGASVINISSMSAAAPMTKVPAY 183 I++FF LS V DLN +GT IPTQIF + M+ + GA VIN+SSMSA +PMTKVPAY Sbjct: 121 ISTFFHLSTDSVQNVFDLNFMGTFIPTQIFTKNMVHQKGAVVINMSSMSAPSPMTKVPAY 180 Query: 184 SAAKAGVNNFTQWLAVHLAEAGIRVNAIAPGFFLTKQNEKLLLREDGELTERSHKIISQT 243 SAAKA + NFT+WLAVH+AEAGIRVNAIAPGFFLT QN+ LLL E+G+ TERSHKIIS T Sbjct: 181 SAAKAAIENFTKWLAVHMAEAGIRVNAIAPGFFLTSQNKNLLLNEEGDYTERSHKIISNT 240 Query: 244 PMRRFGKPEELLGTLLWLADESMSGFVTGITVPVDGGFMAYSGV 287 PMRRFG PE+L GTLLWL D+ MS FVTG+T+PVDGGFMAYSGV Sbjct: 241 PMRRFGTPEDLFGTLLWLVDDHMSAFVTGVTIPVDGGFMAYSGV 284 >Q65L03.1 Q65L03_BACLD YjmF Length = 282 Score = 372 bits (954), Expect = e-101, Method: Compositional matrix adjust. Identities = 181/281 (64%), Positives = 214/281 (76%) Query: 7 LALQPALAGKVAVITGGAGVLGAVMAHELGRQGAKVAILNRTAEKGESLAAGINAAGGQA 66 L + LAGK AVITGG+GVL MA ELGRQG VAILNRT EKGE +A + AGG A Sbjct: 2 LEIHENLAGKTAVITGGSGVLCGAMARELGRQGMNVAILNRTVEKGELVAEAVRKAGGAA 61 Query: 67 VSVACDVTEDSSVHRAAAEVLNHWGPCDLLINGAGGNQPSAVTTKEIFTEGDLENPEITS 126 ++V CDV SV +A V + +G CDLL+NGAGGN P+ T+ E F + I + Sbjct: 62 IAVECDVISQRSVEQAKRVVADRFGGCDLLVNGAGGNHPNGTTSDETFNSEHIFQDNIKT 121 Query: 127 FFELSAAGFLQVMDLNLIGTLIPTQIFARQMLGRPGASVINISSMSAAAPMTKVPAYSAA 186 F+L+ V++LN +G+ IPTQ FA+ ML + GASVINISSMSA PMTKVPAYSAA Sbjct: 122 LFDLNCDDVGSVLNLNFLGSFIPTQQFAKMMLNQKGASVINISSMSAQTPMTKVPAYSAA 181 Query: 187 KAGVNNFTQWLAVHLAEAGIRVNAIAPGFFLTKQNEKLLLREDGELTERSHKIISQTPMR 246 KAG++NFT+WLAV+LAE GIRVNAIAPGFFLT QNE+LLL ++GE T+R+ KIIS TPMR Sbjct: 182 KAGIDNFTKWLAVYLAETGIRVNAIAPGFFLTNQNERLLLDQNGEFTDRAKKIISHTPMR 241 Query: 247 RFGKPEELLGTLLWLADESMSGFVTGITVPVDGGFMAYSGV 287 RFGKPE+LLGTLLWLADE MSGFVTGITVPVDGGF AY+GV Sbjct: 242 RFGKPEDLLGTLLWLADEKMSGFVTGITVPVDGGFTAYAGV 282 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.133 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,661,926,793 Number of extensions: 66247995 Number of successful extensions: 331185 Number of sequences better than 1.0e-05: 26863 Number of HSP's gapped: 291268 Number of HSP's successfully gapped: 26962 Length of query: 287 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 153 Effective length of database: 1,798,280,193 Effective search space: 275136869529 Effective search space used: 275136869529 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)