BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0548.0022.p (221 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q24YR4.1 Q24YR4_DESHY Putative uncharacterized protein 147 6e-34 B8FS19.1 B8FS19_DESHD Ser/Thr and Tyr protein phosphatase (Dual ... 147 8e-34 Q892P9.1 Q892P9_CLOTE Ser/Thr and Tyr protein phosphatase (Dual ... 141 3e-32 B9E175.1 B9E175_CLOK1 Putative uncharacterized protein 125 4e-27 A5N7R4.1 A5N7R4_CLOK5 Predicted phosphatase 125 4e-27 >Q24YR4.1 Q24YR4_DESHY Putative uncharacterized protein Length = 247 Score = 147 bits (371), Expect = 6e-34, Method: Compositional matrix adjust. Identities = 79/171 (46%), Positives = 100/171 (58%) Query: 29 NIFYVTLNHGGGQVNSLVTDIDRQXXXXXXXXXXXXXWYVFITGMLLLHFFKDKKSFGQT 88 NIFY LN+ SLVTD+D+ WY FI G L+ KDKK + +T Sbjct: 28 NIFYALLNNADRGAYSLVTDLDQTIPFLKGFIVPYIIWYFFIFGTLVYFCLKDKKVYYRT 87 Query: 89 LVTLCTGLVACYMIYLQFQTTVPRPQLSSSGVLIHLVEWVYSHDQPFNCFPSIHVFTSYL 148 L+ GL+ACY IY FQT VPRP+L VL LV +YS DQP+N FPSIHV +++L Sbjct: 88 LLAFNLGLLACYGIYFVFQTHVPRPELVGHDVLTKLVALIYSKDQPYNAFPSIHVLSTFL 147 Query: 149 MIKSVQGHAAFSRTFKAAVYFCAWLIICSTLFVKQHVLLDAAGAIALAEIL 199 M+K + + + + VY A LII STLFVKQHV+LD AI L +L Sbjct: 148 MVKGINKCQSKNYLNTSLVYVNAVLIILSTLFVKQHVMLDVLAAIVLGSLL 198 >B8FS19.1 B8FS19_DESHD Ser/Thr and Tyr protein phosphatase (Dual specificity)# Length = 240 Score = 147 bits (370), Expect = 8e-34, Method: Compositional matrix adjust. Identities = 79/171 (46%), Positives = 100/171 (58%) Query: 29 NIFYVTLNHGGGQVNSLVTDIDRQXXXXXXXXXXXXXWYVFITGMLLLHFFKDKKSFGQT 88 NIFY LN+ SLVTD+D+ WY FI G L+ KDKK + +T Sbjct: 21 NIFYALLNNADRGAYSLVTDLDQTIPFLKGFIVPYIIWYFFIFGTLVYFCLKDKKVYYRT 80 Query: 89 LVTLCTGLVACYMIYLQFQTTVPRPQLSSSGVLIHLVEWVYSHDQPFNCFPSIHVFTSYL 148 L+ GL+ACY IY FQT VPRP+L VL LV +YS DQP+N FPSIHV +++L Sbjct: 81 LLAFNLGLLACYGIYFVFQTHVPRPELVGHDVLTKLVALIYSKDQPYNAFPSIHVLSTFL 140 Query: 149 MIKSVQGHAAFSRTFKAAVYFCAWLIICSTLFVKQHVLLDAAGAIALAEIL 199 M+K + + + + VY A LII STLFVKQHV+LD AI L +L Sbjct: 141 MVKGINKCQSKNYLNTSLVYVNAVLIILSTLFVKQHVMLDVLAAIVLGSLL 191 >Q892P9.1 Q892P9_CLOTE Ser/Thr and Tyr protein phosphatase (Dual specificity)# Length = 208 Score = 141 bits (356), Expect = 3e-32, Method: Compositional matrix adjust. Identities = 78/171 (45%), Positives = 99/171 (57%), Gaps = 1/171 (0%) Query: 29 NIFYVTLNHGGGQVNSLVTDIDRQXXXXXXXXXXXXXWYVFITGMLLLHFFKDKKSFGQT 88 NIFY LN+ V L+T ID WY F+ LL KD K + +T Sbjct: 21 NIFYTLLNNNTRGVFQLITTIDNNIPFMKEFIIPYILWYPFMILSLLFLCLKDLKVYYRT 80 Query: 89 LVTLCTGLVACYMIYLQFQTTVPRPQLSSSGVLIHLVEWVYSHDQPFNCFPSIHVFTSYL 148 L+TL L++CY+IY FQTTVPRP + + + +L +Y D+PFNCFPSIHV TSYL Sbjct: 81 LITLNLCLLSCYLIYYFFQTTVPRPDIYGNDIFSYLTLLIYKFDKPFNCFPSIHVVTSYL 140 Query: 149 MIKSVQGHAAFSRTFKAAVYFCAWLIICSTLFVKQHVLLDAAGAIALAEIL 199 MIK V A S+ K V F + II STLFVKQHV++D AI L +I+ Sbjct: 141 MIKGVNSVAC-SKKIKHLVAFMSISIIISTLFVKQHVIMDVISAILLGDIM 190 >B9E175.1 B9E175_CLOK1 Putative uncharacterized protein Length = 217 Score = 125 bits (313), Expect = 4e-27, Method: Compositional matrix adjust. Identities = 67/172 (38%), Positives = 96/172 (55%), Gaps = 1/172 (0%) Query: 29 NIFYVTLNHGGGQVNSLVTDIDRQXXXXXXXXXXXXXWYVFITGMLLLHFFKDKKSFGQT 88 N+ Y LN+ +LVT +D+Q WY F+ +L+ F + + +T Sbjct: 21 NVIYRKLNNPLNGCYNLVTFLDKQVPFIKEFIVVYWAWYPFLILVLIYFCFNYRNIYYKT 80 Query: 89 LVTLCTGLVACYMIYLQFQTTVPRPQLSSSGVLIHLVEWVYSHDQPFNCFPSIHVFTSYL 148 ++T+ G++ CYM Y FQTTVPRP L + I +++ Y D+PFNCFPSIHV T+Y Sbjct: 81 MITIILGMLLCYMTYFFFQTTVPRPALYGRDIFIGIIKLTYRWDKPFNCFPSIHVLTTYA 140 Query: 149 MIKSVQGHAAFSRTFKAAVYFCAWLIICSTLFVKQHVLLDAAGAIALAEILH 200 I+ ++ K ++ F II ST FVKQHV+LD AI LAEIL+ Sbjct: 141 AIRGCM-EGVKNKFNKMSINFTGIFIILSTQFVKQHVILDLISAIILAEILY 191 >A5N7R4.1 A5N7R4_CLOK5 Predicted phosphatase Length = 217 Score = 125 bits (313), Expect = 4e-27, Method: Compositional matrix adjust. Identities = 67/172 (38%), Positives = 96/172 (55%), Gaps = 1/172 (0%) Query: 29 NIFYVTLNHGGGQVNSLVTDIDRQXXXXXXXXXXXXXWYVFITGMLLLHFFKDKKSFGQT 88 N+ Y LN+ +LVT +D+Q WY F+ +L+ F + + +T Sbjct: 21 NVIYRKLNNPLNGCYNLVTFLDKQVPFIKEFIVVYWAWYPFLILVLIYFCFNYRNIYYKT 80 Query: 89 LVTLCTGLVACYMIYLQFQTTVPRPQLSSSGVLIHLVEWVYSHDQPFNCFPSIHVFTSYL 148 ++T+ G++ CYM Y FQTTVPRP L + I +++ Y D+PFNCFPSIHV T+Y Sbjct: 81 MITIILGMLLCYMTYFFFQTTVPRPALYGRDIFIGIIKLTYRWDKPFNCFPSIHVLTTYA 140 Query: 149 MIKSVQGHAAFSRTFKAAVYFCAWLIICSTLFVKQHVLLDAAGAIALAEILH 200 I+ ++ K ++ F II ST FVKQHV+LD AI LAEIL+ Sbjct: 141 AIRGCM-EGVKNKFNKMSINFTGIFIILSTQFVKQHVILDLISAIILAEILY 191 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.330 0.139 0.439 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,091,350,182 Number of extensions: 37059944 Number of successful extensions: 120815 Number of sequences better than 1.0e-05: 141 Number of HSP's gapped: 120697 Number of HSP's successfully gapped: 141 Length of query: 221 Length of database: 3,060,696,203 Length adjustment: 131 Effective length of query: 90 Effective length of database: 1,826,543,238 Effective search space: 164388891420 Effective search space used: 164388891420 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 128 (53.9 bits)