BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0548.0004.p (809 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B5JPX8.1 B5JPX8_9BACT Glycosyl hydrolase family 26 171 4e-40 B1ZXJ4.1 B1ZXJ4_OPITP Mannan endo-1,4-beta-mannosidase EC=3.2.1.78 159 2e-36 C1SA60.1 C1SA60_9SPHI Beta-mannanase 155 2e-35 A8Y8G5.1 A8Y8G5_STRAM Putative glycosidase 139 9e-31 B5JDP2.1 B5JDP2_9BACT Glycosyl hydrolase family 26 122 1e-25 >B5JPX8.1 B5JPX8_9BACT Glycosyl hydrolase family 26 Length = 323 Score = 171 bits (432), Expect = 4e-40, Method: Compositional matrix adjust. Identities = 83/203 (40%), Positives = 131/203 (64%), Gaps = 5/203 (2%) Query: 539 NPGATVEAAALLDRLYAATGNTIYTGQHNYYEEPAKWYDKAAELTGYYPALWGSDFAYYT 598 NP A+ EA LL+ LY+ +G++ +GQH + + YPAL+G DF + Sbjct: 3 NPNASPEARELLEFLYSISGDSTLSGQHCVPLVGSTRLQGIEKSVDRYPALFGQDFGFSE 62 Query: 599 GEDMGGL--RQSMIDEAILQGKAGTMVTLTYHQTRPMDPATSGW-ESVTGDVTEAQMTDI 655 G+ RQ+++DEAI + + G +++L +H RP++ + ES+ G++T+ + D+ Sbjct: 63 PGTWDGINYRQNIVDEAIRRHEEGFVISLMWHAVRPIEEEPVRFRESIQGELTDQEWKDL 122 Query: 656 VTPGTELYNQWAAQVDEVAGYLEQLQDEGIPVLWRPYHEMNAEFFWWGAR--PELFKQLW 713 +TPGTE+ +W +QVD +A +L+QL+D +PVLWRPYHEMN +FWWG + + +++LW Sbjct: 123 MTPGTEVNERWKSQVDVIAFFLKQLRDADVPVLWRPYHEMNGAWFWWGKKGGEDGYEKLW 182 Query: 714 HNMYDRLTHLHGLNNLIWVWNPN 736 +YDRLTH H L+NL+WVWN N Sbjct: 183 RMLYDRLTHFHKLDNLVWVWNAN 205 >B1ZXJ4.1 B1ZXJ4_OPITP Mannan endo-1,4-beta-mannosidase EC=3.2.1.78 Length = 506 Score = 159 bits (401), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 86/228 (37%), Positives = 127/228 (55%), Gaps = 15/228 (6%) Query: 522 ISAASALQVTTGKMAPVNPGATVEAAALLDRLYAATGNTIYTGQHNYYEEPAKWYDKAAE 581 ++A A + PV P A+ EA ALL+ LY +G TGQHNY + AA+ Sbjct: 12 LAALCATPTFAARALPVTPDASPEARALLELLYDLSGRHTLTGQHNYPNIKDRNSRFAAQ 71 Query: 582 LTGYYPALWGSDFAYYTG--EDMGGLRQSMIDEAILQGKAGTMVTLTYHQTRPM--DPAT 637 G PA++ +DF + D R ++ E I Q + G +VT+ +H P +P T Sbjct: 72 HIGKMPAVFSTDFGHAVAGNSDSYLARPDIVQECIRQHQLGALVTICWHAVPPTADEPVT 131 Query: 638 ---------SGWESVTGDVTEAQMTDIVTPGTELYNQWAAQVDEVAGYLEQLQDEGIPVL 688 +SV G + + Q D++TPGT L+ +WAAQVD +A +L+QL+ +PVL Sbjct: 132 FRPLPGSDPKALKSVQGQLLDEQFRDVLTPGTALHTKWAAQVDAIAVFLKQLEAAHVPVL 191 Query: 689 WRPYHEMNAEFFWWGARPELF--KQLWHNMYDRLTHLHGLNNLIWVWN 734 WRPYHEMN +FWWG R + ++L+ ++DRL + H L NL+WVW+ Sbjct: 192 WRPYHEMNGGWFWWGGRTGEYSTERLYRQIFDRLVNHHQLKNLVWVWS 239 >C1SA60.1 C1SA60_9SPHI Beta-mannanase Length = 342 Score = 155 bits (392), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 81/189 (42%), Positives = 111/189 (58%), Gaps = 9/189 (4%) Query: 553 LYAATGNTIYTGQHNY--YEEPAKWYDKAAELTGYYPALWGSDFAYYTGEDMGGLRQSMI 610 LY+ G +G HN PAKW ++ TG YPALW DF + +D RQ MI Sbjct: 47 LYSLAGKKTLSGIHNREPNSTPAKWTNEIFTTTGRYPALWSGDFLFQ--QDNIDNRQKMI 104 Query: 611 DEAILQGKAGTMVTLTYHQTRPMDPATSGWES---VTGDVTEAQMTDIVTPGTELYNQWA 667 DEA+ Q + G ++ L +H P + W+ V +T+AQ ++ T GT L +W Sbjct: 105 DEAVRQWEKGAVINLMWHACNPANEEPCAWDDGKGVLSKLTDAQWKELCTDGTVLNLKWK 164 Query: 668 AQVDEVAGYLEQLQDEGIPVLWRPYHEMNAEFFWWGARP--ELFKQLWHNMYDRLTHLHG 725 A+VDE+ YL+QLQD+G+ VLWRP HEMN FWWG RP E +L+ M+D ++ + G Sbjct: 165 ARVDEICIYLKQLQDKGVEVLWRPMHEMNQGKFWWGGRPGAEGTVKLYRMMHDYMSRIKG 224 Query: 726 LNNLIWVWN 734 LNNLIWVW+ Sbjct: 225 LNNLIWVWD 233 >A8Y8G5.1 A8Y8G5_STRAM Putative glycosidase Length = 327 Score = 139 bits (351), Expect = 9e-31, Method: Compositional matrix adjust. Identities = 74/191 (38%), Positives = 112/191 (58%), Gaps = 7/191 (3%) Query: 549 LLDRLYAATGNTIYTGQHNY--YEEPAKWYDKAAELTGYYPALWGSDFAYYTGEDMGGLR 606 +L+ L A +GN I +GQHN P ++ + ++TG YP LWG D + + G R Sbjct: 33 VLNHLRAISGNHIVSGQHNKEPASAPGQYTQQVKDVTGQYPGLWGGDLMFAAADVAG--R 90 Query: 607 QSMIDEAILQGKAGTMVTLTYHQTRPMDPATSGWES-VTGDVTEAQMTDIVTPGTELYNQ 665 Q ++D+A + G++V+LT+H P +T +E V +T AQ + ++T G+ L + Sbjct: 91 QRVVDQARTEWANGSLVSLTWHVCPPTGGSTCAFEGGVKSTLTNAQFSQVLTEGSALNSA 150 Query: 666 WAAQVDEVAGYLEQLQDEGIPVLWRPYHEMNAEFFWWGARP--ELFKQLWHNMYDRLTHL 723 W ++DEV YL+QL++ G+PVL+RP HEMN + WWG RP +L+ D L Sbjct: 151 WKRRLDEVVPYLQQLENAGVPVLFRPLHEMNESWNWWGNRPGANGSARLYQITRDHLAGT 210 Query: 724 HGLNNLIWVWN 734 GL+NLIWVWN Sbjct: 211 KGLDNLIWVWN 221 >B5JDP2.1 B5JDP2_9BACT Glycosyl hydrolase family 26 Length = 405 Score = 122 bits (307), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 88/258 (34%), Positives = 122/258 (47%), Gaps = 56/258 (21%) Query: 535 MAPVNPGATVEAAALLDRLYAATGNTIYTGQHNYY---------------EEPAKWY--- 576 +AP+NP A+ EA L + G I +GQHN+ + P W Sbjct: 28 IAPINPKASEEAINLYRFIQDVQGEYILSGQHNFVGKGSEYTEQLEALTGKSPVVWGADF 87 Query: 577 ------DKAAELTGYYPA------LWGSDFAYYTGEDMG--------------------- 603 DKA E PA + G E++G Sbjct: 88 SFTAKGDKAMEFQHAGPANLPAIDVEGVREVVEKLEELGLEWPPPPEMFPELEFLDITIE 147 Query: 604 GLRQSMIDEAILQGKAGTMVTLTYHQTRPMDPATSGWESV--TGDV-TEAQMTDIVTPGT 660 R+S I+EA + G ++TL +H P D SV G++ ++ + ++VT GT Sbjct: 148 DARESTIEEAKRRHAMGHIITLMWHGCYPTDAYPCDGLSVWAEGNLPSDEEWNELVTDGT 207 Query: 661 ELYNQWAAQVDEVAGYLEQLQDEGIPVLWRPYHEMNAEFFWWGARP--ELFKQLWHNMYD 718 EL W +D +A YL++L+D+ IPVLWRPYHEMN E+FWWG + + FK+LW YD Sbjct: 208 ELNEAWKVGMDVIAVYLKELRDDNIPVLWRPYHEMNGEWFWWGHKKGEDGFKRLWLMTYD 267 Query: 719 RLTHLHGLNNLIWVWNPN 736 LT+ H LNNLIWVWN N Sbjct: 268 YLTNHHELNNLIWVWNAN 285 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.132 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 5,219,516,096 Number of extensions: 230424630 Number of successful extensions: 538784 Number of sequences better than 1.0e-05: 162 Number of HSP's gapped: 538949 Number of HSP's successfully gapped: 164 Length of query: 809 Length of database: 3,060,696,203 Length adjustment: 144 Effective length of query: 665 Effective length of database: 1,704,070,043 Effective search space: 1133206578595 Effective search space used: 1133206578595 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 136 (57.0 bits)