BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0547.0061.p (160 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DBT4.1 B1DBT4_9BACL NUDIX hydrolase 235 9e-61 B3KH57.1 B3KH57_9BACI NUDIX hydrolase 229 5e-59 Q04EP7.1 Q04EP7_OENOB ADP-ribose pyrophosphatase 172 1e-41 A0NKP6.1 A0NKP6_OENOE Putative uncharacterized protein 172 1e-41 Q39QF2.1 Q39QF2_GEOMG NUDIX hydrolase 107 5e-22 >B1DBT4.1 B1DBT4_9BACL NUDIX hydrolase Length = 160 Score = 235 bits (599), Expect = 9e-61, Method: Compositional matrix adjust. Identities = 109/160 (68%), Positives = 132/160 (82%) Query: 1 MAMPTHIVAVGGIVENEQGEVLLVKTYHGGWVFPGGQVEAGENLIEALIREIKEESGIDT 60 M MPTHI+AVGGIVEN++G+VLLVKT H GWVFPGGQVE GENL +ALIREI+EESGI Sbjct: 1 MTMPTHILAVGGIVENDRGDVLLVKTQHDGWVFPGGQVEVGENLNDALIREIEEESGIAC 60 Query: 61 EVSRLIGVYSNTATYKWHDGVTDVPTKVMLDYICKPVGGQLSTSDETSDSRWVAKDTALE 120 VS+LIGVYSNT YKWHDGVTDVPTK+MLD++C+PVGG L TS+ETS+ WV K+ L+ Sbjct: 61 VVSQLIGVYSNTCMYKWHDGVTDVPTKLMLDFVCRPVGGSLQTSEETSEVCWVRKERVLD 120 Query: 121 MITSPAIRTRFQAYLEFAGNVAYIVYETKPEFKVAMSREI 160 +I S AIRTR+QAYL+F G V Y+ Y TKP+F + + R + Sbjct: 121 LIHSLAIRTRYQAYLDFNGTVTYMDYVTKPDFVIKLERNV 160 >B3KH57.1 B3KH57_9BACI NUDIX hydrolase Length = 159 Score = 229 bits (585), Expect = 5e-59, Method: Compositional matrix adjust. Identities = 108/160 (67%), Positives = 131/160 (81%), Gaps = 1/160 (0%) Query: 1 MAMPTHIVAVGGIVENEQGEVLLVKTYHGGWVFPGGQVEAGENLIEALIREIKEESGIDT 60 M MPTHIVAVGG+VENE GE+LLVK GWVFPGGQVEAGENL++ALIREIKEESGI+T Sbjct: 1 MMMPTHIVAVGGVVENEHGEILLVKD-RNGWVFPGGQVEAGENLMDALIREIKEESGIET 59 Query: 61 EVSRLIGVYSNTATYKWHDGVTDVPTKVMLDYICKPVGGQLSTSDETSDSRWVAKDTALE 120 +S LIGVYSNT+TY H GV VPTKVM+D++CKP GG+L TS+ET+D RW+ KD L+ Sbjct: 60 IISHLIGVYSNTSTYPGHSGVAVVPTKVMMDFVCKPTGGELRTSEETTDCRWIHKDEVLQ 119 Query: 121 MITSPAIRTRFQAYLEFAGNVAYIVYETKPEFKVAMSREI 160 IT+PAIRTRFQ+YL+F + Y+ Y TKPEF++ + R I Sbjct: 120 YITAPAIRTRFQSYLDFNRALVYMEYITKPEFEIKLRRNI 159 >Q04EP7.1 Q04EP7_OENOB ADP-ribose pyrophosphatase Length = 181 Score = 172 bits (435), Expect = 1e-41, Method: Compositional matrix adjust. Identities = 83/160 (51%), Positives = 107/160 (66%) Query: 1 MAMPTHIVAVGGIVENEQGEVLLVKTYHGGWVFPGGQVEAGENLIEALIREIKEESGIDT 60 M PTHIVAVG +V NE E+LLVKT+ GW PGGQVE GENLI+AL RE++EESGI+ Sbjct: 22 MKDPTHIVAVGAVVLNEDQEILLVKTFFRGWEIPGGQVENGENLIDALKREVREESGIEI 81 Query: 61 EVSRLIGVYSNTATYKWHDGVTDVPTKVMLDYICKPVGGQLSTSDETSDSRWVAKDTALE 120 V +LIGVYSN +V TKV+LD++C+ G+LS S+ETS SRW+ K+ L+ Sbjct: 82 RVDKLIGVYSNIKKSDPLGSKKNVTTKVILDFVCQKKSGKLSISNETSVSRWIPKNKVLD 141 Query: 121 MITSPAIRTRFQAYLEFAGNVAYIVYETKPEFKVAMSREI 160 +I+ P + RFQ YL F G V Y+ Y +P F V + I Sbjct: 142 LISYPIYQERFQNYLNFNGEVNYLAYFNEPNFAVQFRQMI 181 >A0NKP6.1 A0NKP6_OENOE Putative uncharacterized protein Length = 181 Score = 172 bits (435), Expect = 1e-41, Method: Compositional matrix adjust. Identities = 83/160 (51%), Positives = 107/160 (66%) Query: 1 MAMPTHIVAVGGIVENEQGEVLLVKTYHGGWVFPGGQVEAGENLIEALIREIKEESGIDT 60 M PTHIVAVG +V NE E+LLVKT+ GW PGGQVE GENLI+AL RE++EESGI+ Sbjct: 22 MKDPTHIVAVGAVVLNEDQEILLVKTFFRGWEIPGGQVENGENLIDALKREVREESGIEI 81 Query: 61 EVSRLIGVYSNTATYKWHDGVTDVPTKVMLDYICKPVGGQLSTSDETSDSRWVAKDTALE 120 V +LIGVYSN +V TKV+LD++C+ G+LS S+ETS SRW+ K+ L+ Sbjct: 82 RVDKLIGVYSNIKKSDPLGSKKNVTTKVILDFVCQKKSGKLSISNETSVSRWIPKNKVLD 141 Query: 121 MITSPAIRTRFQAYLEFAGNVAYIVYETKPEFKVAMSREI 160 +I+ P + RFQ YL F G V Y+ Y +P F V + I Sbjct: 142 LISYPIYQERFQNYLNFNGEVNYLAYFNEPNFAVQFRQMI 181 >Q39QF2.1 Q39QF2_GEOMG NUDIX hydrolase Length = 153 Score = 107 bits (266), Expect = 5e-22, Method: Compositional matrix adjust. Identities = 51/147 (34%), Positives = 85/147 (57%), Gaps = 10/147 (6%) Query: 4 PTHIVAVGGIVENEQGEVLLVKTYHGGWVFPGGQVEAGENLIEALIREIKEESGIDTEVS 63 P HIV VG ++ N GE+LL++ + GW P G+VEAGE +++AL RE++EE+G++ + Sbjct: 7 PVHIVVVGCLIRNGLGEILLIRHHKRGWEIPQGRVEAGEGIVDALRREVREETGVEIKPG 66 Query: 64 RLIGVYSNTATYKWHDGVTDVPTKVMLDYICKPVGGQLSTSDETSDSRWVAKDTALEMIT 123 L V+S + P ++L ++ G+L+ SDET + W ++ +E++ Sbjct: 67 PLTAVWSKVSP----------PASLILTFLADYAEGELAPSDETPELGWFSEREGVELVA 116 Query: 124 SPAIRTRFQAYLEFAGNVAYIVYETKP 150 P R R +A L+++G VA Y T P Sbjct: 117 HPVTRDRLRALLDYSGTVACFSYATNP 143 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.133 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 964,014,494 Number of extensions: 38173932 Number of successful extensions: 114991 Number of sequences better than 1.0e-05: 886 Number of HSP's gapped: 115720 Number of HSP's successfully gapped: 886 Length of query: 160 Length of database: 3,060,696,203 Length adjustment: 121 Effective length of query: 39 Effective length of database: 1,920,753,388 Effective search space: 74909382132 Effective search space used: 74909382132 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 125 (52.8 bits)