BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0547.0060.p (359 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C2WZC4.1 C2WZC4_BACCE Peptidase M50 128 7e-28 C2USW6.1 C2USW6_BACCE Peptidase M50 128 7e-28 C3EIC7.1 C3EIC7_BACTK Peptidase M50 127 2e-27 C2WJZ7.1 C2WJZ7_BACCE Peptidase M50 127 2e-27 C2MYD9.1 C2MYD9_BACCE Peptidase M50 127 2e-27 >C2WZC4.1 C2WZC4_BACCE Peptidase M50 Length = 365 Score = 128 bits (322), Expect = 7e-28, Method: Compositional matrix adjust. Identities = 97/342 (28%), Positives = 155/342 (45%), Gaps = 31/342 (9%) Query: 32 IISLLKLGKIAGPLISMMFSIWAYALIYPWQFAIGFVLLLFVHELGHVIAAKRIGLPVSA 91 + ++ KL K + + SM S+ AYA+IY W+F + V LLFVHE+GH+ AA+R G+P S Sbjct: 26 VFAIFKLAKFS-TVFSMFLSLGAYAVIYGWKFGVALVYLLFVHEMGHLWAARRKGIPTSP 84 Query: 92 PLFIPFLGALITMKKQPLDAKMEAYVAFGGPVLGSAGXXXXXXXXXXXHSPLLYSLAYVG 151 +FIPF+GALI MK+ P +AK EAY+A+ GP+ G P + +G Sbjct: 85 AIFIPFMGALIGMKEMPKNAKDEAYIAYMGPLFGLLSFLPAIPLYMITKEPFWALVILLG 144 Query: 152 FXXXXXXXXXXXXXDGGRIATAVTRXXXXXXXXXXXXXXXYLKSILFLIIWVLFAYDLY- 210 DGGRI + V+ Y KSIL I+++ +LY Sbjct: 145 SMINFFNLIPVSPLDGGRIISVVSTKIWGAGLVLLLGYSIYFKSILGGFIFIIGCMELYR 204 Query: 211 ------------------KKYISRKKNSQMRTLLLRFLIPVEHLREQGYLIPGPEHTREL 252 K+Y+++ + T + I + H + ++ EH +EL Sbjct: 205 VIKRDEPIKELGYRIDGMKEYVAKLEEELKETGAVHRTIYMMH--HEMNVLRQREHEKEL 262 Query: 253 PFTTYSDLDRQQYLGIRYESLDYYGTARLPVQSIIDKVKLTRLEHITEETGLHLNALCEV 312 ++ ++L ++E LDY +P + D+ + HI E + L E Sbjct: 263 KTGELQRMEVLEHLLPKFEPLDY-----VPYEDEKDEYTI----HIREAFEVAERQLNEW 313 Query: 313 QYTVFENDKYYDVPASSRWKYGAAYAVLAGGIGYLMYLVHVV 354 + + + YY V A ++W A Y L +GY Y ++V Sbjct: 314 EKEKVQKENYYKVDAKTKWTVFACYIGLIAILGYTAYEGYIV 355 >C2USW6.1 C2USW6_BACCE Peptidase M50 Length = 365 Score = 128 bits (322), Expect = 7e-28, Method: Compositional matrix adjust. Identities = 97/342 (28%), Positives = 155/342 (45%), Gaps = 31/342 (9%) Query: 32 IISLLKLGKIAGPLISMMFSIWAYALIYPWQFAIGFVLLLFVHELGHVIAAKRIGLPVSA 91 + ++ KL K + + SM S+ AYA+IY W+F + V LLFVHE+GH+ AA+R G+P S Sbjct: 26 VFAIFKLAKFS-TVFSMFLSLGAYAVIYGWKFGVALVYLLFVHEMGHLWAARRKGIPTSP 84 Query: 92 PLFIPFLGALITMKKQPLDAKMEAYVAFGGPVLGSAGXXXXXXXXXXXHSPLLYSLAYVG 151 +FIPF+GALI MK+ P +AK EAY+A+ GP+ G P + +G Sbjct: 85 AIFIPFMGALIGMKEMPKNAKDEAYIAYMGPLFGLLSFLPAIPLYMITKEPFWALVILLG 144 Query: 152 FXXXXXXXXXXXXXDGGRIATAVTRXXXXXXXXXXXXXXXYLKSILFLIIWVLFAYDLY- 210 DGGRI + V+ Y KSIL I+++ +LY Sbjct: 145 SMINFFNLIPVSPLDGGRIISVVSTKIWGAGLVLLLGYSIYFKSILGGFIFIIGCMELYR 204 Query: 211 ------------------KKYISRKKNSQMRTLLLRFLIPVEHLREQGYLIPGPEHTREL 252 K+Y+++ + T + I + H + ++ EH +EL Sbjct: 205 VIKRDEPIKELGYRIDGMKEYVAKLEEELKETGAVHRTIYMMH--HEMNVLRQREHEKEL 262 Query: 253 PFTTYSDLDRQQYLGIRYESLDYYGTARLPVQSIIDKVKLTRLEHITEETGLHLNALCEV 312 ++ ++L ++E LDY +P + D+ + HI E + L E Sbjct: 263 KTGELQRMEVLEHLLPKFEPLDY-----VPYEDEKDEYTI----HIREAFEVAERQLNEW 313 Query: 313 QYTVFENDKYYDVPASSRWKYGAAYAVLAGGIGYLMYLVHVV 354 + + + YY V A ++W A Y L +GY Y ++V Sbjct: 314 EKEKVQKENYYKVDAKTKWTVFACYIGLIAILGYTAYEGYIV 355 >C3EIC7.1 C3EIC7_BACTK Peptidase M50 Length = 365 Score = 127 bits (318), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 100/347 (28%), Positives = 153/347 (44%), Gaps = 41/347 (11%) Query: 32 IISLLKLGKIAGPLISMMFSIWAYALIYPWQFAIGFVLLLFVHELGHVIAAKRIGLPVSA 91 + ++ KL K + + SM S+ AYA++Y W+F + + LLFVHE+GH+ AAKR G+P S Sbjct: 26 VFAIFKLAKFS-TVFSMFLSLGAYAVLYGWKFGVALIYLLFVHEMGHLWAAKRKGIPTSP 84 Query: 92 PLFIPFLGALITMKKQPLDAKMEAYVAFGGPVLGSAGXXXXXXXXXXXHSPLLYSLAYVG 151 +FIPF+GALI MK+ P +AK EAY+A+ GP+ G P + +G Sbjct: 85 AIFIPFMGALIGMKEMPKNAKDEAYIAYMGPLFGLLSFLPAIPLYIMTKEPFWALVILLG 144 Query: 152 FXXXXXXXXXXXXXDGGRIATAVTRXXXXXXXXXXXXXXXYLKSILFLIIWVLFAYDLY- 210 DGGRI + V+ Y KSIL I ++ +LY Sbjct: 145 SMINFFNLIPVSPLDGGRIISVVSTKIWGAGLILLLGYSIYFKSILGGFIVIIGCMELYR 204 Query: 211 ------------------KKYISRKKNSQMRTLLLRFLIPVEH-----LREQGYLIPGPE 247 K+Y++R + T + I V H LR++ E Sbjct: 205 VIKRDEPIKELGHKIDGMKEYVTRLEEELKETGAVHRTIYVMHHEMNALRQR-------E 257 Query: 248 HTRELPFTTYSDLDRQQYLGIRYESLDYYGTARLPVQSIIDKVKLTRLEHITEETGLHLN 307 +EL ++ +YL ++E LDY +P + D+ + HI E Sbjct: 258 REKELQIGELQKMEVLEYLLPKFEPLDY-----VPYEDEKDEYTI----HIREAFEASER 308 Query: 308 ALCEVQYTVFENDKYYDVPASSRWKYGAAYAVLAGGIGYLMYLVHVV 354 L E + + + YY V A ++W A Y L +GY Y ++V Sbjct: 309 KLNEWKTEKEQQENYYKVDAKTKWTVFACYIGLMAILGYTAYEGYIV 355 >C2WJZ7.1 C2WJZ7_BACCE Peptidase M50 Length = 365 Score = 127 bits (318), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 100/347 (28%), Positives = 153/347 (44%), Gaps = 41/347 (11%) Query: 32 IISLLKLGKIAGPLISMMFSIWAYALIYPWQFAIGFVLLLFVHELGHVIAAKRIGLPVSA 91 + ++ KL K + + SM S+ AYA++Y W+F + + LLFVHE+GH+ AAKR G+P S Sbjct: 26 VFAIFKLAKFS-TVFSMFLSLGAYAVLYGWKFGVALIYLLFVHEMGHLWAAKRKGIPTSP 84 Query: 92 PLFIPFLGALITMKKQPLDAKMEAYVAFGGPVLGSAGXXXXXXXXXXXHSPLLYSLAYVG 151 +FIPF+GALI MK+ P +AK EAY+A+ GP+ G P + +G Sbjct: 85 AIFIPFMGALIGMKEMPKNAKDEAYIAYMGPLFGLLSFLPAIPLYIMTKEPFWALVILLG 144 Query: 152 FXXXXXXXXXXXXXDGGRIATAVTRXXXXXXXXXXXXXXXYLKSILFLIIWVLFAYDLY- 210 DGGRI + V+ Y KSIL I ++ +LY Sbjct: 145 SMINFFNLIPVSPLDGGRIISVVSTKIWGAGLILLLGYSIYFKSILGGFIVIIGCMELYR 204 Query: 211 ------------------KKYISRKKNSQMRTLLLRFLIPVEH-----LREQGYLIPGPE 247 K+Y++R + T + I V H LR++ E Sbjct: 205 VIKRDEPIKELGHKIDGMKEYVTRLEEELKETGAVHRTIYVMHHEMNALRQR-------E 257 Query: 248 HTRELPFTTYSDLDRQQYLGIRYESLDYYGTARLPVQSIIDKVKLTRLEHITEETGLHLN 307 +EL ++ +YL ++E LDY +P + D+ + HI E Sbjct: 258 REKELQIGELQKMEVLEYLLPKFEPLDY-----VPYEDEKDEYTI----HIREAFEASER 308 Query: 308 ALCEVQYTVFENDKYYDVPASSRWKYGAAYAVLAGGIGYLMYLVHVV 354 L E + + + YY V A ++W A Y L +GY Y ++V Sbjct: 309 KLNEWKTEKEQQENYYKVDAKTKWTVFACYIGLMAILGYTAYEGYIV 355 >C2MYD9.1 C2MYD9_BACCE Peptidase M50 Length = 365 Score = 127 bits (318), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 100/347 (28%), Positives = 153/347 (44%), Gaps = 41/347 (11%) Query: 32 IISLLKLGKIAGPLISMMFSIWAYALIYPWQFAIGFVLLLFVHELGHVIAAKRIGLPVSA 91 + ++ KL K + + SM S+ AYA++Y W+F + + LLFVHE+GH+ AAKR G+P S Sbjct: 26 VFAIFKLAKFS-TVFSMFLSLGAYAVLYGWKFGVALIYLLFVHEMGHLWAAKRKGIPTSP 84 Query: 92 PLFIPFLGALITMKKQPLDAKMEAYVAFGGPVLGSAGXXXXXXXXXXXHSPLLYSLAYVG 151 +FIPF+GALI MK+ P +AK EAY+A+ GP+ G P + +G Sbjct: 85 AIFIPFMGALIGMKEMPKNAKDEAYIAYMGPLFGLLSFLPAIPLYIMTKEPFWALVILLG 144 Query: 152 FXXXXXXXXXXXXXDGGRIATAVTRXXXXXXXXXXXXXXXYLKSILFLIIWVLFAYDLY- 210 DGGRI + V+ Y KSIL I ++ +LY Sbjct: 145 SMINFFNLIPVSPLDGGRIISVVSTKIWGAGLILLLGYSIYFKSILGGFIVIIGCMELYR 204 Query: 211 ------------------KKYISRKKNSQMRTLLLRFLIPVEH-----LREQGYLIPGPE 247 K+Y++R + T + I V H LR++ E Sbjct: 205 VIKRDEPIKELGHKIDGMKEYVTRLEEELKETGAVHRTIYVMHHEMNALRQR-------E 257 Query: 248 HTRELPFTTYSDLDRQQYLGIRYESLDYYGTARLPVQSIIDKVKLTRLEHITEETGLHLN 307 +EL ++ +YL ++E LDY +P + D+ + HI E Sbjct: 258 REKELQIGELQKMEVLEYLLPKFEPLDY-----VPYEDEKDEYTI----HIREAFEASER 308 Query: 308 ALCEVQYTVFENDKYYDVPASSRWKYGAAYAVLAGGIGYLMYLVHVV 354 L E + + + YY V A ++W A Y L +GY Y ++V Sbjct: 309 KLNEWKTEKEQQENYYKVDAKTKWTVFACYIGLMAILGYTAYEGYIV 355 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.325 0.142 0.429 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,814,614,266 Number of extensions: 69699395 Number of successful extensions: 259556 Number of sequences better than 1.0e-05: 114 Number of HSP's gapped: 259476 Number of HSP's successfully gapped: 114 Length of query: 359 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 222 Effective length of database: 1,770,017,148 Effective search space: 392943806856 Effective search space used: 392943806856 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.5 bits) S2: 132 (55.5 bits)